Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples

Autores
Krüger, Alejandra; Lucchesi, Paula Maria Alejandra; Sanso, Andrea Mariel; Etcheverría, Analía Inés; Bustamante, Ana Victoria; Burgán, Julia; Fernández, Luciana; Fernández, Daniel; Leotta, Gerardo Aníbal; Friedrich, Alexander W.; Padola, Nora Lía; Rossen, John W. A.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a.
Instituto de Genética Veterinaria
Materia
Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
O26:H11
Genetic characterization
Microarray
MLVA
Hemolytic uremic syndrome
Cattle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/100009
Acceder
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samplesKrüger, AlejandraLucchesi, Paula Maria AlejandraSanso, Andrea MarielEtcheverría, Analía InésBustamante, Ana VictoriaBurgán, JuliaFernández, LucianaFernández, DanielLeotta, Gerardo AníbalFriedrich, Alexander W.Padola, Nora LíaRossen, John W. A.Ciencias VeterinariasShiga toxin-producing Escherichia coliO26:H11Genetic characterizationMicroarrayMLVAHemolytic uremic syndromeCattleThe Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a.Instituto de Genética Veterinaria2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/100009enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/11804info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fcimb.2015.00074/fullinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612136/info:eu-repo/semantics/altIdentifier/issn/2235-2988info:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2015.00074info:eu-repo/semantics/altIdentifier/hdl/11336/11804info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:11:45Zoai:sedici.unlp.edu.ar:10915/100009Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:11:46.248SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
spellingShingle Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
Krüger, Alejandra
Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
O26:H11
Genetic characterization
Microarray
MLVA
Hemolytic uremic syndrome
Cattle
title_short Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_full Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_fullStr Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_full_unstemmed Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_sort Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
dc.creator.none.fl_str_mv Krüger, Alejandra
Lucchesi, Paula Maria Alejandra
Sanso, Andrea Mariel
Etcheverría, Analía Inés
Bustamante, Ana Victoria
Burgán, Julia
Fernández, Luciana
Fernández, Daniel
Leotta, Gerardo Aníbal
Friedrich, Alexander W.
Padola, Nora Lía
Rossen, John W. A.
author Krüger, Alejandra
author_facet Krüger, Alejandra
Lucchesi, Paula Maria Alejandra
Sanso, Andrea Mariel
Etcheverría, Analía Inés
Bustamante, Ana Victoria
Burgán, Julia
Fernández, Luciana
Fernández, Daniel
Leotta, Gerardo Aníbal
Friedrich, Alexander W.
Padola, Nora Lía
Rossen, John W. A.
author_role author
author2 Lucchesi, Paula Maria Alejandra
Sanso, Andrea Mariel
Etcheverría, Analía Inés
Bustamante, Ana Victoria
Burgán, Julia
Fernández, Luciana
Fernández, Daniel
Leotta, Gerardo Aníbal
Friedrich, Alexander W.
Padola, Nora Lía
Rossen, John W. A.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
O26:H11
Genetic characterization
Microarray
MLVA
Hemolytic uremic syndrome
Cattle
topic Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
O26:H11
Genetic characterization
Microarray
MLVA
Hemolytic uremic syndrome
Cattle
dc.description.none.fl_txt_mv The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a.
Instituto de Genética Veterinaria
description The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/100009
url http://sedici.unlp.edu.ar/handle/10915/100009
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/11804
info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fcimb.2015.00074/full
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612136/
info:eu-repo/semantics/altIdentifier/issn/2235-2988
info:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2015.00074
info:eu-repo/semantics/altIdentifier/hdl/11336/11804
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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