Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
- Autores
- Krüger, Alejandra; Lucchesi, Paula Maria Alejandra; Sanso, Andrea Mariel; Etcheverría, Analía Inés; Bustamante, Ana Victoria; Burgán, Julia; Fernández, Luciana; Fernández, Daniel; Leotta, Gerardo Aníbal; Friedrich, Alexander W.; Padola, Nora Lía; Rossen, John W. A.
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a.
Instituto de Genética Veterinaria - Materia
-
Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
O26:H11
Genetic characterization
Microarray
MLVA
Hemolytic uremic syndrome
Cattle - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/100009
Ver los metadatos del registro completo
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Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samplesKrüger, AlejandraLucchesi, Paula Maria AlejandraSanso, Andrea MarielEtcheverría, Analía InésBustamante, Ana VictoriaBurgán, JuliaFernández, LucianaFernández, DanielLeotta, Gerardo AníbalFriedrich, Alexander W.Padola, Nora LíaRossen, John W. A.Ciencias VeterinariasShiga toxin-producing Escherichia coliO26:H11Genetic characterizationMicroarrayMLVAHemolytic uremic syndromeCattleThe Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a.Instituto de Genética Veterinaria2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/100009enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/11804info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fcimb.2015.00074/fullinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612136/info:eu-repo/semantics/altIdentifier/issn/2235-2988info:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2015.00074info:eu-repo/semantics/altIdentifier/hdl/11336/11804info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:19:51Zoai:sedici.unlp.edu.ar:10915/100009Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:19:52.215SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title |
Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
spellingShingle |
Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples Krüger, Alejandra Ciencias Veterinarias Shiga toxin-producing Escherichia coli O26:H11 Genetic characterization Microarray MLVA Hemolytic uremic syndrome Cattle |
title_short |
Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title_full |
Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title_fullStr |
Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title_full_unstemmed |
Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title_sort |
Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
dc.creator.none.fl_str_mv |
Krüger, Alejandra Lucchesi, Paula Maria Alejandra Sanso, Andrea Mariel Etcheverría, Analía Inés Bustamante, Ana Victoria Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Aníbal Friedrich, Alexander W. Padola, Nora Lía Rossen, John W. A. |
author |
Krüger, Alejandra |
author_facet |
Krüger, Alejandra Lucchesi, Paula Maria Alejandra Sanso, Andrea Mariel Etcheverría, Analía Inés Bustamante, Ana Victoria Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Aníbal Friedrich, Alexander W. Padola, Nora Lía Rossen, John W. A. |
author_role |
author |
author2 |
Lucchesi, Paula Maria Alejandra Sanso, Andrea Mariel Etcheverría, Analía Inés Bustamante, Ana Victoria Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Aníbal Friedrich, Alexander W. Padola, Nora Lía Rossen, John W. A. |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Veterinarias Shiga toxin-producing Escherichia coli O26:H11 Genetic characterization Microarray MLVA Hemolytic uremic syndrome Cattle |
topic |
Ciencias Veterinarias Shiga toxin-producing Escherichia coli O26:H11 Genetic characterization Microarray MLVA Hemolytic uremic syndrome Cattle |
dc.description.none.fl_txt_mv |
The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a. Instituto de Genética Veterinaria |
description |
The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/100009 |
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http://sedici.unlp.edu.ar/handle/10915/100009 |
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eng |
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eng |
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