Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces

Autores
Oot, Rebecca; Raya, Raul Ricardo; Callaway, Todd R.; Edrington, Tom S.; Kutter, Elizabeth M.; Brabban, Andrew D.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim: To estimate the distribution and prevalence of both Escherichia coli O157 and O157:H7-infecting bacteriophages within a 50 000 head commercial beef feedlot. Methods and Results: Escherichia coli O157 was detected in ∼27% of the individual samples, distributed across seven of the 10 pens screened. In a simple initial screen to detect O157:H7-infecting phages, none were detected in any pen or individual sample. In contrast, after a series of enrichment procedures O157:H7-infecting phages were detected in every pen and in the majority of the samples from most pens; virulent bacteriophages active against E. coli O157:H7 were detected post-enrichment from 39/60 (65%) of the feedlot samples, and 58/60 (∼97%) contained phage that infected E. coli B or O157:H7. Conclusions: The data we present here indicates that we may be grossly underestimating the prevalence of O157:H7-infecting phages in livestock if we simply screen samples and that enrichment screening is required to truly determine the presence of phages in these ecosystems. Significance and Impact of the Study: Our data suggest that O157:H7-infecting phages may play a role in the ecology and transient colonization of cattle by E. coli O157:H7. Further, this and previous data suggest that before starting in vivo pathogen eradication studies using phage or any other regime, test animals should be enrichment screened for phage to avoid erroneous results.
Fil: Oot, Rebecca. The Evergreen State College; Estados Unidos
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Callaway, Todd R.. College Station. Food and Feed Safety Research Unit. Agricultural Research Service; Estados Unidos
Fil: Edrington, Tom S.. College Station. Food and Feed Safety Research Unit. Agricultural Research Service; Estados Unidos
Fil: Kutter, Elizabeth M.. The Evergreen State College; Estados Unidos
Fil: Brabban, Andrew D.. The Evergreen State College; Estados Unidos
Materia
Bacteriophage
Cattle
Escherichia Coli O157:H7
Fecal
Feedlot
Prevalence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56327
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/56327
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle fecesOot, RebeccaRaya, Raul RicardoCallaway, Todd R.Edrington, Tom S.Kutter, Elizabeth M.Brabban, Andrew D.BacteriophageCattleEscherichia Coli O157:H7FecalFeedlotPrevalencehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aim: To estimate the distribution and prevalence of both Escherichia coli O157 and O157:H7-infecting bacteriophages within a 50 000 head commercial beef feedlot. Methods and Results: Escherichia coli O157 was detected in ∼27% of the individual samples, distributed across seven of the 10 pens screened. In a simple initial screen to detect O157:H7-infecting phages, none were detected in any pen or individual sample. In contrast, after a series of enrichment procedures O157:H7-infecting phages were detected in every pen and in the majority of the samples from most pens; virulent bacteriophages active against E. coli O157:H7 were detected post-enrichment from 39/60 (65%) of the feedlot samples, and 58/60 (∼97%) contained phage that infected E. coli B or O157:H7. Conclusions: The data we present here indicates that we may be grossly underestimating the prevalence of O157:H7-infecting phages in livestock if we simply screen samples and that enrichment screening is required to truly determine the presence of phages in these ecosystems. Significance and Impact of the Study: Our data suggest that O157:H7-infecting phages may play a role in the ecology and transient colonization of cattle by E. coli O157:H7. Further, this and previous data suggest that before starting in vivo pathogen eradication studies using phage or any other regime, test animals should be enrichment screened for phage to avoid erroneous results.Fil: Oot, Rebecca. The Evergreen State College; Estados UnidosFil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Callaway, Todd R.. College Station. Food and Feed Safety Research Unit. Agricultural Research Service; Estados UnidosFil: Edrington, Tom S.. College Station. Food and Feed Safety Research Unit. Agricultural Research Service; Estados UnidosFil: Kutter, Elizabeth M.. The Evergreen State College; Estados UnidosFil: Brabban, Andrew D.. The Evergreen State College; Estados UnidosWiley Blackwell Publishing, Inc2007-10-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56327Oot, Rebecca; Raya, Raul Ricardo; Callaway, Todd R.; Edrington, Tom S.; Kutter, Elizabeth M.; et al.; Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 45; 4; 14-10-2007; 445-4530266-82541472-765XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2007.02211.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1472-765X.2007.02211.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:50:58Zoai:ri.conicet.gov.ar:11336/56327instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:50:59.02CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces
title Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces
spellingShingle Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces
Oot, Rebecca
Bacteriophage
Cattle
Escherichia Coli O157:H7
Fecal
Feedlot
Prevalence
title_short Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces
title_full Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces
title_fullStr Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces
title_full_unstemmed Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces
title_sort Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces
dc.creator.none.fl_str_mv Oot, Rebecca
Raya, Raul Ricardo
Callaway, Todd R.
Edrington, Tom S.
Kutter, Elizabeth M.
Brabban, Andrew D.
author Oot, Rebecca
author_facet Oot, Rebecca
Raya, Raul Ricardo
Callaway, Todd R.
Edrington, Tom S.
Kutter, Elizabeth M.
Brabban, Andrew D.
author_role author
author2 Raya, Raul Ricardo
Callaway, Todd R.
Edrington, Tom S.
Kutter, Elizabeth M.
Brabban, Andrew D.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacteriophage
Cattle
Escherichia Coli O157:H7
Fecal
Feedlot
Prevalence
topic Bacteriophage
Cattle
Escherichia Coli O157:H7
Fecal
Feedlot
Prevalence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aim: To estimate the distribution and prevalence of both Escherichia coli O157 and O157:H7-infecting bacteriophages within a 50 000 head commercial beef feedlot. Methods and Results: Escherichia coli O157 was detected in ∼27% of the individual samples, distributed across seven of the 10 pens screened. In a simple initial screen to detect O157:H7-infecting phages, none were detected in any pen or individual sample. In contrast, after a series of enrichment procedures O157:H7-infecting phages were detected in every pen and in the majority of the samples from most pens; virulent bacteriophages active against E. coli O157:H7 were detected post-enrichment from 39/60 (65%) of the feedlot samples, and 58/60 (∼97%) contained phage that infected E. coli B or O157:H7. Conclusions: The data we present here indicates that we may be grossly underestimating the prevalence of O157:H7-infecting phages in livestock if we simply screen samples and that enrichment screening is required to truly determine the presence of phages in these ecosystems. Significance and Impact of the Study: Our data suggest that O157:H7-infecting phages may play a role in the ecology and transient colonization of cattle by E. coli O157:H7. Further, this and previous data suggest that before starting in vivo pathogen eradication studies using phage or any other regime, test animals should be enrichment screened for phage to avoid erroneous results.
Fil: Oot, Rebecca. The Evergreen State College; Estados Unidos
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Callaway, Todd R.. College Station. Food and Feed Safety Research Unit. Agricultural Research Service; Estados Unidos
Fil: Edrington, Tom S.. College Station. Food and Feed Safety Research Unit. Agricultural Research Service; Estados Unidos
Fil: Kutter, Elizabeth M.. The Evergreen State College; Estados Unidos
Fil: Brabban, Andrew D.. The Evergreen State College; Estados Unidos
description Aim: To estimate the distribution and prevalence of both Escherichia coli O157 and O157:H7-infecting bacteriophages within a 50 000 head commercial beef feedlot. Methods and Results: Escherichia coli O157 was detected in ∼27% of the individual samples, distributed across seven of the 10 pens screened. In a simple initial screen to detect O157:H7-infecting phages, none were detected in any pen or individual sample. In contrast, after a series of enrichment procedures O157:H7-infecting phages were detected in every pen and in the majority of the samples from most pens; virulent bacteriophages active against E. coli O157:H7 were detected post-enrichment from 39/60 (65%) of the feedlot samples, and 58/60 (∼97%) contained phage that infected E. coli B or O157:H7. Conclusions: The data we present here indicates that we may be grossly underestimating the prevalence of O157:H7-infecting phages in livestock if we simply screen samples and that enrichment screening is required to truly determine the presence of phages in these ecosystems. Significance and Impact of the Study: Our data suggest that O157:H7-infecting phages may play a role in the ecology and transient colonization of cattle by E. coli O157:H7. Further, this and previous data suggest that before starting in vivo pathogen eradication studies using phage or any other regime, test animals should be enrichment screened for phage to avoid erroneous results.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56327
Oot, Rebecca; Raya, Raul Ricardo; Callaway, Todd R.; Edrington, Tom S.; Kutter, Elizabeth M.; et al.; Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 45; 4; 14-10-2007; 445-453
0266-8254
1472-765X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56327
identifier_str_mv Oot, Rebecca; Raya, Raul Ricardo; Callaway, Todd R.; Edrington, Tom S.; Kutter, Elizabeth M.; et al.; Prevalence of Escherichia coli O157 and O157:H7-infecting bacteriophages in feedlot cattle feces; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 45; 4; 14-10-2007; 445-453
0266-8254
1472-765X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2007.02211.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1472-765X.2007.02211.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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