Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars

Autores
Basile, Silvana Marisol Lujan; Burrell, Mike M.; Walker, Heather J.; Cardozo, Jorge Alejos; Steels, Chloe; Kallenberg, Felix; Tognetti, Jorge Alberto; Dalla Valle, Horacio R.; Rogers, William John
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In a proof of concept study aimed at showing that metabolites in bread wheat (Triticum aestivum L. ssp. aestivum), phloem exudates have potential as biochemical markers for cultivar discrimination, Argentinean cultivars from three quality groups (groups 1, 2, and 3 of high, intermediate, and low quality, respectively) were grown under two nitrogen (N) availabilities and analysed for metabolic profile by electrospray ionisation mass spectrometry. Data as signal strengths of mass/charge (m/z) values binned to a resolution of 0.2 Daltons were subjected to principal component analysis and orthogonal projections to latent structures discriminant analysis. Certain bins were influential in discriminating groups taken in pairs and some were involved in separating all three groups. In high N availability, group 3 cultivars clustered away from the other cultivars, while group 1 cultivars clustered tightly together; group 2 cultivars were more scattered between group 1 and group 3 cultivars. In low N availability, the cultivars were not clustered as tightly; nonetheless, group 1 cultivars tended to cluster together and mainly separated from those of group 2. m/z values also showed potential for discrimination between N availability. In conclusion, phloem exudate metabolic profiles could provide biochemical markers for selection during breeding and for discerning the effects of N fertiliser application.
Fil: Basile, Silvana Marisol Lujan. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Agronomia. Laboratorio de Biología Funcional y Biotecnología.; Argentina
Fil: Burrell, Mike M.. University of Sheffield; Reino Unido
Fil: Walker, Heather J.. University of Sheffield; Reino Unido
Fil: Cardozo, Jorge Alejos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Agronomia. Laboratorio de Biología Funcional y Biotecnología.; Argentina
Fil: Steels, Chloe. University of Sheffield; Reino Unido
Fil: Kallenberg, Felix. University of Sheffield; Reino Unido
Fil: Tognetti, Jorge Alberto. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Dalla Valle, Horacio R.. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Rogers, William John. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Materia
CULTIVAR
ELECTROSPRAY IONISATION
MASS SPECTROMETRY
NITROGEN
PHLOEM METABOLITES
QUALITY GROUPS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102688
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102688
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat CultivarsBasile, Silvana Marisol LujanBurrell, Mike M.Walker, Heather J.Cardozo, Jorge AlejosSteels, ChloeKallenberg, FelixTognetti, Jorge AlbertoDalla Valle, Horacio R.Rogers, William JohnCULTIVARELECTROSPRAY IONISATIONMASS SPECTROMETRYNITROGENPHLOEM METABOLITESQUALITY GROUPShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4In a proof of concept study aimed at showing that metabolites in bread wheat (Triticum aestivum L. ssp. aestivum), phloem exudates have potential as biochemical markers for cultivar discrimination, Argentinean cultivars from three quality groups (groups 1, 2, and 3 of high, intermediate, and low quality, respectively) were grown under two nitrogen (N) availabilities and analysed for metabolic profile by electrospray ionisation mass spectrometry. Data as signal strengths of mass/charge (m/z) values binned to a resolution of 0.2 Daltons were subjected to principal component analysis and orthogonal projections to latent structures discriminant analysis. Certain bins were influential in discriminating groups taken in pairs and some were involved in separating all three groups. In high N availability, group 3 cultivars clustered away from the other cultivars, while group 1 cultivars clustered tightly together; group 2 cultivars were more scattered between group 1 and group 3 cultivars. In low N availability, the cultivars were not clustered as tightly; nonetheless, group 1 cultivars tended to cluster together and mainly separated from those of group 2. m/z values also showed potential for discrimination between N availability. In conclusion, phloem exudate metabolic profiles could provide biochemical markers for selection during breeding and for discerning the effects of N fertiliser application.Fil: Basile, Silvana Marisol Lujan. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Agronomia. Laboratorio de Biología Funcional y Biotecnología.; ArgentinaFil: Burrell, Mike M.. University of Sheffield; Reino UnidoFil: Walker, Heather J.. University of Sheffield; Reino UnidoFil: Cardozo, Jorge Alejos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Agronomia. Laboratorio de Biología Funcional y Biotecnología.; ArgentinaFil: Steels, Chloe. University of Sheffield; Reino UnidoFil: Kallenberg, Felix. University of Sheffield; Reino UnidoFil: Tognetti, Jorge Alberto. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; ArgentinaFil: Dalla Valle, Horacio R.. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Rogers, William John. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaMDPI2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102688Basile, Silvana Marisol Lujan; Burrell, Mike M.; Walker, Heather J.; Cardozo, Jorge Alejos; Steels, Chloe; et al.; Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars; MDPI; Agronomy; 8; 4; 4-2018; 45-552073-4395CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2073-4395/8/4/45info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy8040045info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:36Zoai:ri.conicet.gov.ar:11336/102688instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:36.315CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars
title Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars
spellingShingle Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars
Basile, Silvana Marisol Lujan
CULTIVAR
ELECTROSPRAY IONISATION
MASS SPECTROMETRY
NITROGEN
PHLOEM METABOLITES
QUALITY GROUPS
title_short Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars
title_full Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars
title_fullStr Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars
title_full_unstemmed Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars
title_sort Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars
dc.creator.none.fl_str_mv Basile, Silvana Marisol Lujan
Burrell, Mike M.
Walker, Heather J.
Cardozo, Jorge Alejos
Steels, Chloe
Kallenberg, Felix
Tognetti, Jorge Alberto
Dalla Valle, Horacio R.
Rogers, William John
author Basile, Silvana Marisol Lujan
author_facet Basile, Silvana Marisol Lujan
Burrell, Mike M.
Walker, Heather J.
Cardozo, Jorge Alejos
Steels, Chloe
Kallenberg, Felix
Tognetti, Jorge Alberto
Dalla Valle, Horacio R.
Rogers, William John
author_role author
author2 Burrell, Mike M.
Walker, Heather J.
Cardozo, Jorge Alejos
Steels, Chloe
Kallenberg, Felix
Tognetti, Jorge Alberto
Dalla Valle, Horacio R.
Rogers, William John
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CULTIVAR
ELECTROSPRAY IONISATION
MASS SPECTROMETRY
NITROGEN
PHLOEM METABOLITES
QUALITY GROUPS
topic CULTIVAR
ELECTROSPRAY IONISATION
MASS SPECTROMETRY
NITROGEN
PHLOEM METABOLITES
QUALITY GROUPS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In a proof of concept study aimed at showing that metabolites in bread wheat (Triticum aestivum L. ssp. aestivum), phloem exudates have potential as biochemical markers for cultivar discrimination, Argentinean cultivars from three quality groups (groups 1, 2, and 3 of high, intermediate, and low quality, respectively) were grown under two nitrogen (N) availabilities and analysed for metabolic profile by electrospray ionisation mass spectrometry. Data as signal strengths of mass/charge (m/z) values binned to a resolution of 0.2 Daltons were subjected to principal component analysis and orthogonal projections to latent structures discriminant analysis. Certain bins were influential in discriminating groups taken in pairs and some were involved in separating all three groups. In high N availability, group 3 cultivars clustered away from the other cultivars, while group 1 cultivars clustered tightly together; group 2 cultivars were more scattered between group 1 and group 3 cultivars. In low N availability, the cultivars were not clustered as tightly; nonetheless, group 1 cultivars tended to cluster together and mainly separated from those of group 2. m/z values also showed potential for discrimination between N availability. In conclusion, phloem exudate metabolic profiles could provide biochemical markers for selection during breeding and for discerning the effects of N fertiliser application.
Fil: Basile, Silvana Marisol Lujan. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Agronomia. Laboratorio de Biología Funcional y Biotecnología.; Argentina
Fil: Burrell, Mike M.. University of Sheffield; Reino Unido
Fil: Walker, Heather J.. University of Sheffield; Reino Unido
Fil: Cardozo, Jorge Alejos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Agronomia. Laboratorio de Biología Funcional y Biotecnología.; Argentina
Fil: Steels, Chloe. University of Sheffield; Reino Unido
Fil: Kallenberg, Felix. University of Sheffield; Reino Unido
Fil: Tognetti, Jorge Alberto. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Dalla Valle, Horacio R.. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Rogers, William John. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
description In a proof of concept study aimed at showing that metabolites in bread wheat (Triticum aestivum L. ssp. aestivum), phloem exudates have potential as biochemical markers for cultivar discrimination, Argentinean cultivars from three quality groups (groups 1, 2, and 3 of high, intermediate, and low quality, respectively) were grown under two nitrogen (N) availabilities and analysed for metabolic profile by electrospray ionisation mass spectrometry. Data as signal strengths of mass/charge (m/z) values binned to a resolution of 0.2 Daltons were subjected to principal component analysis and orthogonal projections to latent structures discriminant analysis. Certain bins were influential in discriminating groups taken in pairs and some were involved in separating all three groups. In high N availability, group 3 cultivars clustered away from the other cultivars, while group 1 cultivars clustered tightly together; group 2 cultivars were more scattered between group 1 and group 3 cultivars. In low N availability, the cultivars were not clustered as tightly; nonetheless, group 1 cultivars tended to cluster together and mainly separated from those of group 2. m/z values also showed potential for discrimination between N availability. In conclusion, phloem exudate metabolic profiles could provide biochemical markers for selection during breeding and for discerning the effects of N fertiliser application.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102688
Basile, Silvana Marisol Lujan; Burrell, Mike M.; Walker, Heather J.; Cardozo, Jorge Alejos; Steels, Chloe; et al.; Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars; MDPI; Agronomy; 8; 4; 4-2018; 45-55
2073-4395
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102688
identifier_str_mv Basile, Silvana Marisol Lujan; Burrell, Mike M.; Walker, Heather J.; Cardozo, Jorge Alejos; Steels, Chloe; et al.; Metabolic Profiling of Phloem Exudates as a Tool to Improve Bread-Wheat Cultivars; MDPI; Agronomy; 8; 4; 4-2018; 45-55
2073-4395
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2073-4395/8/4/45
info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy8040045
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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