First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina

Autores
Lori, Gladys; Malbrán, Ismael; Mourelos, Cecilia Alejandra
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
ArgentinaAnnually, ~20 ha of sweet basil (Ocimum basilicumL.) are cultivated in greenhouses in the green belt area surrounding LaPlata, Argentina, mainly for fresh consumption. In 2004 to 2007, basil plants of cv. Genovese showed wilt symptoms,necrosis of leaves and stems, asymmetrical growth, and discolored vascular tissue in greenhouses in La Plata. In 2007, thesame symptoms were observed on plants of cv. Morada grown from seeds that were produced in Italy. Isolations werecompleted from root, crown, and stem sections of diseased plants of cv. Genovese from three greenhouses in 2004 to 2007,and from commercial seeds, stem sections, flowers, and seeds of diseased plants of cv. Morada in 2007. Seeds andportions of symptomatic tissues were surface­disinfested with 0.5% NaOCl for 1 min, rinsed in sterilized distilled water, airdried, and plated on 2% potato dextrose agar (PDA). Twenty­seven isolates were identified asFusarium oxysporumSchltdl.based on morphological characteristics (4), and the species identification confirmed by PCR assay using aF. oxysporumf.sp.basilici­specific primer pair, Bik 1 and Bik 2 (1). Vegetative compatibility groups (VCGs) were determined for the 27isolates through complementation of nitrate­nonutilizing mutants generated from these isolates (2) and paired with twoItalian tester strains from an international collection (PVS­Fu 220 and PVS­Fu 125, provided by V. Balmas, Univeristà degliStudi di Sassari, Italy). All 27 isolates from Argentina belonged to VCG 0200. This is a unique VCG forF. oxysporumf.sp.basiliciand has been identified in Israeli, American, and Italian isolates of the fungus (3). To fulfill Koch's postulates,pathogenicity tests were conducted with 12 isolates selected to reflect the multiple sources of fungal recovery, includingroot, crown, and stem sections, and leaves of diseased plants of cv. Genovese and commercial seeds, stem sections,flowers, and seeds of cv. Morada. Isolates were each grown on moistened (40% w/w), autoclaved, polished rice for 10days, dried, and ground in a grinder. The number of CFU/g rice was determined by serial dilution plating onto PDA plates.The inoculum was added to autoclaved soil at 104CFU/g dry soil. For each isolate, 8 healthy basil seedlings of each of cvs.Genovese and Morada were planted in pots, each containing 1 liter of inoculated soil. The control treatment consisted of 8basil seedlings of each of the same cultivars planted in autoclaved soil mixed with sterilized, ground, polished rice. Plantswere grown in a greenhouse with natural daylight for 45 to 50 days after inoculation. All inoculated plants showed the samesymptoms described for the original basil plants. No symptoms were observed on the control plants.F. oxysporumf.sp.basiliciwas re­isolated from the vascular tissue of stems of symptomatic plants but not from control plants, and speciesidentification confirmed by PCR assay as previously described. The presence of the pathogen was verified in the seed lotproduced in Italy, suggesting that this could have been a source of inoculum that introduced the pathogen into La Plata,Argentina, as supported by the hypothesis that infested seed resulted in spread of a clonal population ofF. oxysporumf.sp.basiliciinternationally (1). To our knowledge, this is the first report ofF. oxysporumf. sp.basiliciinfecting sweet basilin Argentina.
Fil: Lori, Gladys. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Malbrán, Ismael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Mourelos, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Materia
FUNGI AND OOMYCETES
HERBACEOUS/FLOWERING PLANTS
EPIDEMIOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33049

id CONICETDig_eb08f9717c8e2cc60b2fb70ba00dfc55
oai_identifier_str oai:ri.conicet.gov.ar:11336/33049
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in ArgentinaLori, GladysMalbrán, IsmaelMourelos, Cecilia AlejandraFUNGI AND OOMYCETESHERBACEOUS/FLOWERING PLANTSEPIDEMIOLOGYhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4ArgentinaAnnually, ~20 ha of sweet basil (Ocimum basilicumL.) are cultivated in greenhouses in the green belt area surrounding LaPlata, Argentina, mainly for fresh consumption. In 2004 to 2007, basil plants of cv. Genovese showed wilt symptoms,necrosis of leaves and stems, asymmetrical growth, and discolored vascular tissue in greenhouses in La Plata. In 2007, thesame symptoms were observed on plants of cv. Morada grown from seeds that were produced in Italy. Isolations werecompleted from root, crown, and stem sections of diseased plants of cv. Genovese from three greenhouses in 2004 to 2007,and from commercial seeds, stem sections, flowers, and seeds of diseased plants of cv. Morada in 2007. Seeds andportions of symptomatic tissues were surface­disinfested with 0.5% NaOCl for 1 min, rinsed in sterilized distilled water, airdried, and plated on 2% potato dextrose agar (PDA). Twenty­seven isolates were identified asFusarium oxysporumSchltdl.based on morphological characteristics (4), and the species identification confirmed by PCR assay using aF. oxysporumf.sp.basilici­specific primer pair, Bik 1 and Bik 2 (1). Vegetative compatibility groups (VCGs) were determined for the 27isolates through complementation of nitrate­nonutilizing mutants generated from these isolates (2) and paired with twoItalian tester strains from an international collection (PVS­Fu 220 and PVS­Fu 125, provided by V. Balmas, Univeristà degliStudi di Sassari, Italy). All 27 isolates from Argentina belonged to VCG 0200. This is a unique VCG forF. oxysporumf.sp.basiliciand has been identified in Israeli, American, and Italian isolates of the fungus (3). To fulfill Koch's postulates,pathogenicity tests were conducted with 12 isolates selected to reflect the multiple sources of fungal recovery, includingroot, crown, and stem sections, and leaves of diseased plants of cv. Genovese and commercial seeds, stem sections,flowers, and seeds of cv. Morada. Isolates were each grown on moistened (40% w/w), autoclaved, polished rice for 10days, dried, and ground in a grinder. The number of CFU/g rice was determined by serial dilution plating onto PDA plates.The inoculum was added to autoclaved soil at 104CFU/g dry soil. For each isolate, 8 healthy basil seedlings of each of cvs.Genovese and Morada were planted in pots, each containing 1 liter of inoculated soil. The control treatment consisted of 8basil seedlings of each of the same cultivars planted in autoclaved soil mixed with sterilized, ground, polished rice. Plantswere grown in a greenhouse with natural daylight for 45 to 50 days after inoculation. All inoculated plants showed the samesymptoms described for the original basil plants. No symptoms were observed on the control plants.F. oxysporumf.sp.basiliciwas re­isolated from the vascular tissue of stems of symptomatic plants but not from control plants, and speciesidentification confirmed by PCR assay as previously described. The presence of the pathogen was verified in the seed lotproduced in Italy, suggesting that this could have been a source of inoculum that introduced the pathogen into La Plata,Argentina, as supported by the hypothesis that infested seed resulted in spread of a clonal population ofF. oxysporumf.sp.basiliciinternationally (1). To our knowledge, this is the first report ofF. oxysporumf. sp.basiliciinfecting sweet basilin Argentina.Fil: Lori, Gladys. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaFil: Malbrán, Ismael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaFil: Mourelos, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaAmerican Phytopathological Society2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33049Lori, Gladys; Mourelos, Cecilia Alejandra; Malbrán, Ismael; First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina; American Phytopathological Society; Plant Disease; 98; 10; 10-2014; 1432-14320191-2917CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-03-14-0243-PDNinfo:eu-repo/semantics/altIdentifier/doi/10.1094/PDIS-03-14-0243-PDNinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:30Zoai:ri.conicet.gov.ar:11336/33049instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:30.458CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina
title First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina
spellingShingle First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina
Lori, Gladys
FUNGI AND OOMYCETES
HERBACEOUS/FLOWERING PLANTS
EPIDEMIOLOGY
title_short First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina
title_full First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina
title_fullStr First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina
title_full_unstemmed First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina
title_sort First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina
dc.creator.none.fl_str_mv Lori, Gladys
Malbrán, Ismael
Mourelos, Cecilia Alejandra
author Lori, Gladys
author_facet Lori, Gladys
Malbrán, Ismael
Mourelos, Cecilia Alejandra
author_role author
author2 Malbrán, Ismael
Mourelos, Cecilia Alejandra
author2_role author
author
dc.subject.none.fl_str_mv FUNGI AND OOMYCETES
HERBACEOUS/FLOWERING PLANTS
EPIDEMIOLOGY
topic FUNGI AND OOMYCETES
HERBACEOUS/FLOWERING PLANTS
EPIDEMIOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv ArgentinaAnnually, ~20 ha of sweet basil (Ocimum basilicumL.) are cultivated in greenhouses in the green belt area surrounding LaPlata, Argentina, mainly for fresh consumption. In 2004 to 2007, basil plants of cv. Genovese showed wilt symptoms,necrosis of leaves and stems, asymmetrical growth, and discolored vascular tissue in greenhouses in La Plata. In 2007, thesame symptoms were observed on plants of cv. Morada grown from seeds that were produced in Italy. Isolations werecompleted from root, crown, and stem sections of diseased plants of cv. Genovese from three greenhouses in 2004 to 2007,and from commercial seeds, stem sections, flowers, and seeds of diseased plants of cv. Morada in 2007. Seeds andportions of symptomatic tissues were surface­disinfested with 0.5% NaOCl for 1 min, rinsed in sterilized distilled water, airdried, and plated on 2% potato dextrose agar (PDA). Twenty­seven isolates were identified asFusarium oxysporumSchltdl.based on morphological characteristics (4), and the species identification confirmed by PCR assay using aF. oxysporumf.sp.basilici­specific primer pair, Bik 1 and Bik 2 (1). Vegetative compatibility groups (VCGs) were determined for the 27isolates through complementation of nitrate­nonutilizing mutants generated from these isolates (2) and paired with twoItalian tester strains from an international collection (PVS­Fu 220 and PVS­Fu 125, provided by V. Balmas, Univeristà degliStudi di Sassari, Italy). All 27 isolates from Argentina belonged to VCG 0200. This is a unique VCG forF. oxysporumf.sp.basiliciand has been identified in Israeli, American, and Italian isolates of the fungus (3). To fulfill Koch's postulates,pathogenicity tests were conducted with 12 isolates selected to reflect the multiple sources of fungal recovery, includingroot, crown, and stem sections, and leaves of diseased plants of cv. Genovese and commercial seeds, stem sections,flowers, and seeds of cv. Morada. Isolates were each grown on moistened (40% w/w), autoclaved, polished rice for 10days, dried, and ground in a grinder. The number of CFU/g rice was determined by serial dilution plating onto PDA plates.The inoculum was added to autoclaved soil at 104CFU/g dry soil. For each isolate, 8 healthy basil seedlings of each of cvs.Genovese and Morada were planted in pots, each containing 1 liter of inoculated soil. The control treatment consisted of 8basil seedlings of each of the same cultivars planted in autoclaved soil mixed with sterilized, ground, polished rice. Plantswere grown in a greenhouse with natural daylight for 45 to 50 days after inoculation. All inoculated plants showed the samesymptoms described for the original basil plants. No symptoms were observed on the control plants.F. oxysporumf.sp.basiliciwas re­isolated from the vascular tissue of stems of symptomatic plants but not from control plants, and speciesidentification confirmed by PCR assay as previously described. The presence of the pathogen was verified in the seed lotproduced in Italy, suggesting that this could have been a source of inoculum that introduced the pathogen into La Plata,Argentina, as supported by the hypothesis that infested seed resulted in spread of a clonal population ofF. oxysporumf.sp.basiliciinternationally (1). To our knowledge, this is the first report ofF. oxysporumf. sp.basiliciinfecting sweet basilin Argentina.
Fil: Lori, Gladys. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Malbrán, Ismael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Mourelos, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
description ArgentinaAnnually, ~20 ha of sweet basil (Ocimum basilicumL.) are cultivated in greenhouses in the green belt area surrounding LaPlata, Argentina, mainly for fresh consumption. In 2004 to 2007, basil plants of cv. Genovese showed wilt symptoms,necrosis of leaves and stems, asymmetrical growth, and discolored vascular tissue in greenhouses in La Plata. In 2007, thesame symptoms were observed on plants of cv. Morada grown from seeds that were produced in Italy. Isolations werecompleted from root, crown, and stem sections of diseased plants of cv. Genovese from three greenhouses in 2004 to 2007,and from commercial seeds, stem sections, flowers, and seeds of diseased plants of cv. Morada in 2007. Seeds andportions of symptomatic tissues were surface­disinfested with 0.5% NaOCl for 1 min, rinsed in sterilized distilled water, airdried, and plated on 2% potato dextrose agar (PDA). Twenty­seven isolates were identified asFusarium oxysporumSchltdl.based on morphological characteristics (4), and the species identification confirmed by PCR assay using aF. oxysporumf.sp.basilici­specific primer pair, Bik 1 and Bik 2 (1). Vegetative compatibility groups (VCGs) were determined for the 27isolates through complementation of nitrate­nonutilizing mutants generated from these isolates (2) and paired with twoItalian tester strains from an international collection (PVS­Fu 220 and PVS­Fu 125, provided by V. Balmas, Univeristà degliStudi di Sassari, Italy). All 27 isolates from Argentina belonged to VCG 0200. This is a unique VCG forF. oxysporumf.sp.basiliciand has been identified in Israeli, American, and Italian isolates of the fungus (3). To fulfill Koch's postulates,pathogenicity tests were conducted with 12 isolates selected to reflect the multiple sources of fungal recovery, includingroot, crown, and stem sections, and leaves of diseased plants of cv. Genovese and commercial seeds, stem sections,flowers, and seeds of cv. Morada. Isolates were each grown on moistened (40% w/w), autoclaved, polished rice for 10days, dried, and ground in a grinder. The number of CFU/g rice was determined by serial dilution plating onto PDA plates.The inoculum was added to autoclaved soil at 104CFU/g dry soil. For each isolate, 8 healthy basil seedlings of each of cvs.Genovese and Morada were planted in pots, each containing 1 liter of inoculated soil. The control treatment consisted of 8basil seedlings of each of the same cultivars planted in autoclaved soil mixed with sterilized, ground, polished rice. Plantswere grown in a greenhouse with natural daylight for 45 to 50 days after inoculation. All inoculated plants showed the samesymptoms described for the original basil plants. No symptoms were observed on the control plants.F. oxysporumf.sp.basiliciwas re­isolated from the vascular tissue of stems of symptomatic plants but not from control plants, and speciesidentification confirmed by PCR assay as previously described. The presence of the pathogen was verified in the seed lotproduced in Italy, suggesting that this could have been a source of inoculum that introduced the pathogen into La Plata,Argentina, as supported by the hypothesis that infested seed resulted in spread of a clonal population ofF. oxysporumf.sp.basiliciinternationally (1). To our knowledge, this is the first report ofF. oxysporumf. sp.basiliciinfecting sweet basilin Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33049
Lori, Gladys; Mourelos, Cecilia Alejandra; Malbrán, Ismael; First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina; American Phytopathological Society; Plant Disease; 98; 10; 10-2014; 1432-1432
0191-2917
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33049
identifier_str_mv Lori, Gladys; Mourelos, Cecilia Alejandra; Malbrán, Ismael; First Report of Fusarium Wilt of Basil Caused by Fusarium oxysporum f. sp. basilici in Argentina; American Phytopathological Society; Plant Disease; 98; 10; 10-2014; 1432-1432
0191-2917
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-03-14-0243-PDN
info:eu-repo/semantics/altIdentifier/doi/10.1094/PDIS-03-14-0243-PDN
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613610070867968
score 13.070432