Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina

Autores
Malbrán, Ismael; Mourelos, Cecilia Alejandra; Mitidieri, Mariel Silvina; Ronco, B. L.; Lori, Gladys
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The green belt area surrounding the city of La Plata, Argentina, produces more than 70% (around 280 ha) of the lettuce (Lactuca sativa L.) cultivated under greenhouse for fresh consumption in the country. In February 2011, April 2012 and December 2013, butterhead lettuce plants from cv. Lores showing wilt and stunted growth symptoms, red-to-brown discoloration of vascular tissues and yellow leaves were found in greenhouses in La Plata. Sections of tap root, crown and stem from symptomatic plants showing dark-brown streaking of the vascular tissue were surface sterilized and isolations were made. A total of 21 monosporic isolates obtained from different lettuce production fields were identified as Fusarium oxysporum Schltdl. based on morphological characteristics (2). Vegetative compatibility group (VCG) analysis (1) was conducted on 11 of these isolates and all isolates belonged to the same VCG. To fulfil Koch?s postulates, two pathogenicity tests were conducted with these 11 isolates in winter (July 2012) and summer (December 2013). Healthy 20-day-old butterhead lettuce seedlings of two cultivars (Reina de Mayo and Lores in the winter and summer tests, respectively) were inoculated by dipping the roots of each plant in a spore suspensión (≈ 3 × 105 CFU mL-1), planted in 1 L pots containing autoclaved 26 soil and grown in a greenhousewith only natural daylight. Control treatments were prepared by dipping the seedling roots insterilized distilled water. All inoculated plants showed wilt symptoms 15 to 20 days after inoculation (dai) and 45 to 50 dai in the summer and winter pathogenicity tests, respectively. The delay in the appearance of symptoms observed during the winter test is consistent with the effect of planting date on the development and final incidence of Fusarium wilt of lettuce reported by Matheron et al. (3). No symptoms were observed in control treatments. F. oxysporum was reisolatedfrom vascular tissues of the stems of symptomatic plants and the formae speciales lactucae J.C. Hubb. & Gerik was confirmed by polymerase chain reaction (PCR) using the specific primer pair GYCF1 and R943 (4). The identification of only one VCG for the tested isolates agrees with the hypothesis of seed transmission of the pathogen, which might explain the dissemination of Fusarium wilt of lettuce in geographically distant areas (2). Studies are being carried out to determine the race of these Argentinian isolates of F. oxysporum f. sp. lactucae. To our knowledge, this is the first report of F. oxysporum f. sp. lactucae infecting lettuce in Argentina.
Fil: Malbrán, Ismael. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Mourelos, Cecilia Alejandra. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte. Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Ronco, B. L.. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Lori, Gladys. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Materia
Lettuce
Fusarium Wilt
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/32530
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in ArgentinaMalbrán, IsmaelMourelos, Cecilia AlejandraMitidieri, Mariel SilvinaRonco, B. L.Lori, GladysLettuceFusarium Wilthttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1The green belt area surrounding the city of La Plata, Argentina, produces more than 70% (around 280 ha) of the lettuce (Lactuca sativa L.) cultivated under greenhouse for fresh consumption in the country. In February 2011, April 2012 and December 2013, butterhead lettuce plants from cv. Lores showing wilt and stunted growth symptoms, red-to-brown discoloration of vascular tissues and yellow leaves were found in greenhouses in La Plata. Sections of tap root, crown and stem from symptomatic plants showing dark-brown streaking of the vascular tissue were surface sterilized and isolations were made. A total of 21 monosporic isolates obtained from different lettuce production fields were identified as Fusarium oxysporum Schltdl. based on morphological characteristics (2). Vegetative compatibility group (VCG) analysis (1) was conducted on 11 of these isolates and all isolates belonged to the same VCG. To fulfil Koch?s postulates, two pathogenicity tests were conducted with these 11 isolates in winter (July 2012) and summer (December 2013). Healthy 20-day-old butterhead lettuce seedlings of two cultivars (Reina de Mayo and Lores in the winter and summer tests, respectively) were inoculated by dipping the roots of each plant in a spore suspensión (≈ 3 × 105 CFU mL-1), planted in 1 L pots containing autoclaved 26 soil and grown in a greenhousewith only natural daylight. Control treatments were prepared by dipping the seedling roots insterilized distilled water. All inoculated plants showed wilt symptoms 15 to 20 days after inoculation (dai) and 45 to 50 dai in the summer and winter pathogenicity tests, respectively. The delay in the appearance of symptoms observed during the winter test is consistent with the effect of planting date on the development and final incidence of Fusarium wilt of lettuce reported by Matheron et al. (3). No symptoms were observed in control treatments. F. oxysporum was reisolatedfrom vascular tissues of the stems of symptomatic plants and the formae speciales lactucae J.C. Hubb. & Gerik was confirmed by polymerase chain reaction (PCR) using the specific primer pair GYCF1 and R943 (4). The identification of only one VCG for the tested isolates agrees with the hypothesis of seed transmission of the pathogen, which might explain the dissemination of Fusarium wilt of lettuce in geographically distant areas (2). Studies are being carried out to determine the race of these Argentinian isolates of F. oxysporum f. sp. lactucae. To our knowledge, this is the first report of F. oxysporum f. sp. lactucae infecting lettuce in Argentina.Fil: Malbrán, Ismael. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaFil: Mourelos, Cecilia Alejandra. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaFil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte. Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Ronco, B. L.. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaFil: Lori, Gladys. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaAmerican Phytopathological Society2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32530Mitidieri, Mariel Silvina; Malbrán, Ismael; Lori, Gladys; Ronco, B. L.; Mourelos, Cecilia Alejandra; Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina; American Phytopathological Society; Plant Disease; 98; 9; 5-2014; 12810191-2917CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/PDIS-04-14-0372-PDNinfo:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-14-0372-PDNinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:04Zoai:ri.conicet.gov.ar:11336/32530instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:04.862CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina
title Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina
spellingShingle Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina
Malbrán, Ismael
Lettuce
Fusarium Wilt
title_short Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina
title_full Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina
title_fullStr Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina
title_full_unstemmed Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina
title_sort Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina
dc.creator.none.fl_str_mv Malbrán, Ismael
Mourelos, Cecilia Alejandra
Mitidieri, Mariel Silvina
Ronco, B. L.
Lori, Gladys
author Malbrán, Ismael
author_facet Malbrán, Ismael
Mourelos, Cecilia Alejandra
Mitidieri, Mariel Silvina
Ronco, B. L.
Lori, Gladys
author_role author
author2 Mourelos, Cecilia Alejandra
Mitidieri, Mariel Silvina
Ronco, B. L.
Lori, Gladys
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lettuce
Fusarium Wilt
topic Lettuce
Fusarium Wilt
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The green belt area surrounding the city of La Plata, Argentina, produces more than 70% (around 280 ha) of the lettuce (Lactuca sativa L.) cultivated under greenhouse for fresh consumption in the country. In February 2011, April 2012 and December 2013, butterhead lettuce plants from cv. Lores showing wilt and stunted growth symptoms, red-to-brown discoloration of vascular tissues and yellow leaves were found in greenhouses in La Plata. Sections of tap root, crown and stem from symptomatic plants showing dark-brown streaking of the vascular tissue were surface sterilized and isolations were made. A total of 21 monosporic isolates obtained from different lettuce production fields were identified as Fusarium oxysporum Schltdl. based on morphological characteristics (2). Vegetative compatibility group (VCG) analysis (1) was conducted on 11 of these isolates and all isolates belonged to the same VCG. To fulfil Koch?s postulates, two pathogenicity tests were conducted with these 11 isolates in winter (July 2012) and summer (December 2013). Healthy 20-day-old butterhead lettuce seedlings of two cultivars (Reina de Mayo and Lores in the winter and summer tests, respectively) were inoculated by dipping the roots of each plant in a spore suspensión (≈ 3 × 105 CFU mL-1), planted in 1 L pots containing autoclaved 26 soil and grown in a greenhousewith only natural daylight. Control treatments were prepared by dipping the seedling roots insterilized distilled water. All inoculated plants showed wilt symptoms 15 to 20 days after inoculation (dai) and 45 to 50 dai in the summer and winter pathogenicity tests, respectively. The delay in the appearance of symptoms observed during the winter test is consistent with the effect of planting date on the development and final incidence of Fusarium wilt of lettuce reported by Matheron et al. (3). No symptoms were observed in control treatments. F. oxysporum was reisolatedfrom vascular tissues of the stems of symptomatic plants and the formae speciales lactucae J.C. Hubb. & Gerik was confirmed by polymerase chain reaction (PCR) using the specific primer pair GYCF1 and R943 (4). The identification of only one VCG for the tested isolates agrees with the hypothesis of seed transmission of the pathogen, which might explain the dissemination of Fusarium wilt of lettuce in geographically distant areas (2). Studies are being carried out to determine the race of these Argentinian isolates of F. oxysporum f. sp. lactucae. To our knowledge, this is the first report of F. oxysporum f. sp. lactucae infecting lettuce in Argentina.
Fil: Malbrán, Ismael. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Mourelos, Cecilia Alejandra. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte. Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Ronco, B. L.. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: Lori, Gladys. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
description The green belt area surrounding the city of La Plata, Argentina, produces more than 70% (around 280 ha) of the lettuce (Lactuca sativa L.) cultivated under greenhouse for fresh consumption in the country. In February 2011, April 2012 and December 2013, butterhead lettuce plants from cv. Lores showing wilt and stunted growth symptoms, red-to-brown discoloration of vascular tissues and yellow leaves were found in greenhouses in La Plata. Sections of tap root, crown and stem from symptomatic plants showing dark-brown streaking of the vascular tissue were surface sterilized and isolations were made. A total of 21 monosporic isolates obtained from different lettuce production fields were identified as Fusarium oxysporum Schltdl. based on morphological characteristics (2). Vegetative compatibility group (VCG) analysis (1) was conducted on 11 of these isolates and all isolates belonged to the same VCG. To fulfil Koch?s postulates, two pathogenicity tests were conducted with these 11 isolates in winter (July 2012) and summer (December 2013). Healthy 20-day-old butterhead lettuce seedlings of two cultivars (Reina de Mayo and Lores in the winter and summer tests, respectively) were inoculated by dipping the roots of each plant in a spore suspensión (≈ 3 × 105 CFU mL-1), planted in 1 L pots containing autoclaved 26 soil and grown in a greenhousewith only natural daylight. Control treatments were prepared by dipping the seedling roots insterilized distilled water. All inoculated plants showed wilt symptoms 15 to 20 days after inoculation (dai) and 45 to 50 dai in the summer and winter pathogenicity tests, respectively. The delay in the appearance of symptoms observed during the winter test is consistent with the effect of planting date on the development and final incidence of Fusarium wilt of lettuce reported by Matheron et al. (3). No symptoms were observed in control treatments. F. oxysporum was reisolatedfrom vascular tissues of the stems of symptomatic plants and the formae speciales lactucae J.C. Hubb. & Gerik was confirmed by polymerase chain reaction (PCR) using the specific primer pair GYCF1 and R943 (4). The identification of only one VCG for the tested isolates agrees with the hypothesis of seed transmission of the pathogen, which might explain the dissemination of Fusarium wilt of lettuce in geographically distant areas (2). Studies are being carried out to determine the race of these Argentinian isolates of F. oxysporum f. sp. lactucae. To our knowledge, this is the first report of F. oxysporum f. sp. lactucae infecting lettuce in Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
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info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32530
Mitidieri, Mariel Silvina; Malbrán, Ismael; Lori, Gladys; Ronco, B. L.; Mourelos, Cecilia Alejandra; Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina; American Phytopathological Society; Plant Disease; 98; 9; 5-2014; 1281
0191-2917
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32530
identifier_str_mv Mitidieri, Mariel Silvina; Malbrán, Ismael; Lori, Gladys; Ronco, B. L.; Mourelos, Cecilia Alejandra; Fusarium Wilt of Lettuce Caused by Fusarium oxysporum f. sp. lactucae in Argentina; American Phytopathological Society; Plant Disease; 98; 9; 5-2014; 1281
0191-2917
CONICET Digital
CONICET
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language eng
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dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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