Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5

Autores
Nieto Peñalver, Carlos Gabriel; Bichara, Laura Cecilia; Marino, Damian Jose Gabriel; Castellanos, Lucia Ines; Irazusta, Verónica Patricia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Gluconacetobacter diazotrophicus is an acid-tolerant nitrogen- fixing Alphaproteobacterium first found in association with sugarcane. It has also been isolated from rice, coffee and tea, among others crops. The recent sequencing of the G. diazotrophicus PAL5 genome shows the presence of one luxI homolog. These genes encode LuxI-type enzymes responsible for the synthesis of N- acylhomoserine lactones (AHLs), the main quorum sensing molecules in gram negative bacteria. The objective of this work was the detection and identification of AHLs produced by G. diazotrophicus PAL5. The strain was cultured aerobically, and extracts were prepared with acidified ethyl acetate. Samples were analyzed by thin layer chromatography developed with the biosensor Agrobacterium tumefaciens NTL4 (pCF218) (pCF372). Results show that G. diazotrophicus PAL5 produce at least two types of AHLs under the assayed conditions. Short-chain AHLs could be detected since early exponential growth phase, and medium-chain AHLs were detected in mid- and late-exponential growth phase. The results suggest that the luxI homolog in G. diazotrophicus PAL5 is expressed and quorum sensing molecules are produced and secreted. Similar to other bacteria, production of AHLs in G. diazotrophicus PAL5 could serves as a signaling mechanism among members of this genus or as inter kingdom signals.
Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Bichara, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Irazusta, Verónica Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
GLUCONACETOBACTER
QUORUM-SENSING
AUTOINDUCTOR
LACTONAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194943
Acceder
id CONICETDig_eb07038debc36feae31bb74723813d24
oai_identifier_str oai:ri.conicet.gov.ar:11336/194943
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5Nieto Peñalver, Carlos GabrielBichara, Laura CeciliaMarino, Damian Jose GabrielCastellanos, Lucia InesIrazusta, Verónica PatriciaGLUCONACETOBACTERQUORUM-SENSINGAUTOINDUCTORLACTONAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Gluconacetobacter diazotrophicus is an acid-tolerant nitrogen- fixing Alphaproteobacterium first found in association with sugarcane. It has also been isolated from rice, coffee and tea, among others crops. The recent sequencing of the G. diazotrophicus PAL5 genome shows the presence of one luxI homolog. These genes encode LuxI-type enzymes responsible for the synthesis of N- acylhomoserine lactones (AHLs), the main quorum sensing molecules in gram negative bacteria. The objective of this work was the detection and identification of AHLs produced by G. diazotrophicus PAL5. The strain was cultured aerobically, and extracts were prepared with acidified ethyl acetate. Samples were analyzed by thin layer chromatography developed with the biosensor Agrobacterium tumefaciens NTL4 (pCF218) (pCF372). Results show that G. diazotrophicus PAL5 produce at least two types of AHLs under the assayed conditions. Short-chain AHLs could be detected since early exponential growth phase, and medium-chain AHLs were detected in mid- and late-exponential growth phase. The results suggest that the luxI homolog in G. diazotrophicus PAL5 is expressed and quorum sensing molecules are produced and secreted. Similar to other bacteria, production of AHLs in G. diazotrophicus PAL5 could serves as a signaling mechanism among members of this genus or as inter kingdom signals.Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Bichara, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Irazusta, Verónica Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPuerto MadrynArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Investigación en Bioquímica y Biología Molecular2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194943Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 109-1090327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:47Zoai:ri.conicet.gov.ar:11336/194943instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:47.76CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5
title Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5
spellingShingle Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5
Nieto Peñalver, Carlos Gabriel
GLUCONACETOBACTER
QUORUM-SENSING
AUTOINDUCTOR
LACTONAS
title_short Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5
title_full Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5
title_fullStr Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5
title_full_unstemmed Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5
title_sort Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5
dc.creator.none.fl_str_mv Nieto Peñalver, Carlos Gabriel
Bichara, Laura Cecilia
Marino, Damian Jose Gabriel
Castellanos, Lucia Ines
Irazusta, Verónica Patricia
author Nieto Peñalver, Carlos Gabriel
author_facet Nieto Peñalver, Carlos Gabriel
Bichara, Laura Cecilia
Marino, Damian Jose Gabriel
Castellanos, Lucia Ines
Irazusta, Verónica Patricia
author_role author
author2 Bichara, Laura Cecilia
Marino, Damian Jose Gabriel
Castellanos, Lucia Ines
Irazusta, Verónica Patricia
author2_role author
author
author
author
dc.subject.none.fl_str_mv GLUCONACETOBACTER
QUORUM-SENSING
AUTOINDUCTOR
LACTONAS
topic GLUCONACETOBACTER
QUORUM-SENSING
AUTOINDUCTOR
LACTONAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Gluconacetobacter diazotrophicus is an acid-tolerant nitrogen- fixing Alphaproteobacterium first found in association with sugarcane. It has also been isolated from rice, coffee and tea, among others crops. The recent sequencing of the G. diazotrophicus PAL5 genome shows the presence of one luxI homolog. These genes encode LuxI-type enzymes responsible for the synthesis of N- acylhomoserine lactones (AHLs), the main quorum sensing molecules in gram negative bacteria. The objective of this work was the detection and identification of AHLs produced by G. diazotrophicus PAL5. The strain was cultured aerobically, and extracts were prepared with acidified ethyl acetate. Samples were analyzed by thin layer chromatography developed with the biosensor Agrobacterium tumefaciens NTL4 (pCF218) (pCF372). Results show that G. diazotrophicus PAL5 produce at least two types of AHLs under the assayed conditions. Short-chain AHLs could be detected since early exponential growth phase, and medium-chain AHLs were detected in mid- and late-exponential growth phase. The results suggest that the luxI homolog in G. diazotrophicus PAL5 is expressed and quorum sensing molecules are produced and secreted. Similar to other bacteria, production of AHLs in G. diazotrophicus PAL5 could serves as a signaling mechanism among members of this genus or as inter kingdom signals.
Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Bichara, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Irazusta, Verónica Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Gluconacetobacter diazotrophicus is an acid-tolerant nitrogen- fixing Alphaproteobacterium first found in association with sugarcane. It has also been isolated from rice, coffee and tea, among others crops. The recent sequencing of the G. diazotrophicus PAL5 genome shows the presence of one luxI homolog. These genes encode LuxI-type enzymes responsible for the synthesis of N- acylhomoserine lactones (AHLs), the main quorum sensing molecules in gram negative bacteria. The objective of this work was the detection and identification of AHLs produced by G. diazotrophicus PAL5. The strain was cultured aerobically, and extracts were prepared with acidified ethyl acetate. Samples were analyzed by thin layer chromatography developed with the biosensor Agrobacterium tumefaciens NTL4 (pCF218) (pCF372). Results show that G. diazotrophicus PAL5 produce at least two types of AHLs under the assayed conditions. Short-chain AHLs could be detected since early exponential growth phase, and medium-chain AHLs were detected in mid- and late-exponential growth phase. The results suggest that the luxI homolog in G. diazotrophicus PAL5 is expressed and quorum sensing molecules are produced and secreted. Similar to other bacteria, production of AHLs in G. diazotrophicus PAL5 could serves as a signaling mechanism among members of this genus or as inter kingdom signals.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194943
Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 109-109
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194943
identifier_str_mv Detection of acylhomoserine lactones in cultures of Gluconacetobacter diazotrophicus PAL5; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 109-109
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614019454861312
score 13.070432

Publicaciones similares