Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones

Autores
Amaya, Susana del Valle; Pereira, José A.; Borkosky, Susana Alicia; Valdéz, Juan Carlos; Bardon, Alicia del Valle; Arena, Mario Eduardo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract Six sesquiterpene lactones (SLs) of the goyazensolide and isogoyazensolide-type isolated from the Argentine herb Centratherum punctatum were evaluated on their ability to inhibit virulence factors of Pseudomonas aeruginosa ATCC 27853. Although compounds were able to completely inhibit bacterial growth at 200 µg/ml, the SLs do altered biofilm formation, elastase activity, and production of N-acyl-homoserinelactones (AHL) which are known quorum sensing autoinducers at lower concentration. Compounds 2, 3, and 5 displayed significant inhibitory effects on P. aeruginosa biofilm formation at 0.5 µg/ml being compound 3 (1.32 µM) the most potent (42%). Compounds 2,3, 4, 5, and 6, inhibited 39, 44, 42, 32 and 35% the production of AHLs at 100 µg/ml and inhibited by more than 50% the elastase activity at 0.5 µg/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents.
Fil: Amaya, Susana del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Pereira, José A.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Borkosky, Susana Alicia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Arena, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Materia
Centratherum Punctatum
Biofilm
Quorum Sensing
Sesquiterpene Lactones
Pseudomonas Aeruginosa
Elastase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/77653
Acceder
id CONICETDig_22694a0904db1ada1d5184e8f673b785
oai_identifier_str oai:ri.conicet.gov.ar:11336/77653
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactonesAmaya, Susana del VallePereira, José A.Borkosky, Susana AliciaValdéz, Juan CarlosBardon, Alicia del ValleArena, Mario EduardoCentratherum PunctatumBiofilmQuorum SensingSesquiterpene LactonesPseudomonas AeruginosaElastasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Abstract Six sesquiterpene lactones (SLs) of the goyazensolide and isogoyazensolide-type isolated from the Argentine herb Centratherum punctatum were evaluated on their ability to inhibit virulence factors of Pseudomonas aeruginosa ATCC 27853. Although compounds were able to completely inhibit bacterial growth at 200 µg/ml, the SLs do altered biofilm formation, elastase activity, and production of N-acyl-homoserinelactones (AHL) which are known quorum sensing autoinducers at lower concentration. Compounds 2, 3, and 5 displayed significant inhibitory effects on P. aeruginosa biofilm formation at 0.5 µg/ml being compound 3 (1.32 µM) the most potent (42%). Compounds 2,3, 4, 5, and 6, inhibited 39, 44, 42, 32 and 35% the production of AHLs at 100 µg/ml and inhibited by more than 50% the elastase activity at 0.5 µg/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents.Fil: Amaya, Susana del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Pereira, José A.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Borkosky, Susana Alicia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Arena, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaElsevier Gmbh2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/77653Amaya, Susana del Valle; Pereira, José A.; Borkosky, Susana Alicia; Valdéz, Juan Carlos; Bardon, Alicia del Valle; et al.; Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones; Elsevier Gmbh; Phytomedicine; 19; 13; 9-2012; 1173-11770944-7113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2012.07.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0944711312002139?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:51Zoai:ri.conicet.gov.ar:11336/77653instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:51.574CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones
title Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones
spellingShingle Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones
Amaya, Susana del Valle
Centratherum Punctatum
Biofilm
Quorum Sensing
Sesquiterpene Lactones
Pseudomonas Aeruginosa
Elastase
title_short Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones
title_full Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones
title_fullStr Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones
title_full_unstemmed Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones
title_sort Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones
dc.creator.none.fl_str_mv Amaya, Susana del Valle
Pereira, José A.
Borkosky, Susana Alicia
Valdéz, Juan Carlos
Bardon, Alicia del Valle
Arena, Mario Eduardo
author Amaya, Susana del Valle
author_facet Amaya, Susana del Valle
Pereira, José A.
Borkosky, Susana Alicia
Valdéz, Juan Carlos
Bardon, Alicia del Valle
Arena, Mario Eduardo
author_role author
author2 Pereira, José A.
Borkosky, Susana Alicia
Valdéz, Juan Carlos
Bardon, Alicia del Valle
Arena, Mario Eduardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Centratherum Punctatum
Biofilm
Quorum Sensing
Sesquiterpene Lactones
Pseudomonas Aeruginosa
Elastase
topic Centratherum Punctatum
Biofilm
Quorum Sensing
Sesquiterpene Lactones
Pseudomonas Aeruginosa
Elastase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Abstract Six sesquiterpene lactones (SLs) of the goyazensolide and isogoyazensolide-type isolated from the Argentine herb Centratherum punctatum were evaluated on their ability to inhibit virulence factors of Pseudomonas aeruginosa ATCC 27853. Although compounds were able to completely inhibit bacterial growth at 200 µg/ml, the SLs do altered biofilm formation, elastase activity, and production of N-acyl-homoserinelactones (AHL) which are known quorum sensing autoinducers at lower concentration. Compounds 2, 3, and 5 displayed significant inhibitory effects on P. aeruginosa biofilm formation at 0.5 µg/ml being compound 3 (1.32 µM) the most potent (42%). Compounds 2,3, 4, 5, and 6, inhibited 39, 44, 42, 32 and 35% the production of AHLs at 100 µg/ml and inhibited by more than 50% the elastase activity at 0.5 µg/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents.
Fil: Amaya, Susana del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Pereira, José A.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Borkosky, Susana Alicia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Arena, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
description Abstract Six sesquiterpene lactones (SLs) of the goyazensolide and isogoyazensolide-type isolated from the Argentine herb Centratherum punctatum were evaluated on their ability to inhibit virulence factors of Pseudomonas aeruginosa ATCC 27853. Although compounds were able to completely inhibit bacterial growth at 200 µg/ml, the SLs do altered biofilm formation, elastase activity, and production of N-acyl-homoserinelactones (AHL) which are known quorum sensing autoinducers at lower concentration. Compounds 2, 3, and 5 displayed significant inhibitory effects on P. aeruginosa biofilm formation at 0.5 µg/ml being compound 3 (1.32 µM) the most potent (42%). Compounds 2,3, 4, 5, and 6, inhibited 39, 44, 42, 32 and 35% the production of AHLs at 100 µg/ml and inhibited by more than 50% the elastase activity at 0.5 µg/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/77653
Amaya, Susana del Valle; Pereira, José A.; Borkosky, Susana Alicia; Valdéz, Juan Carlos; Bardon, Alicia del Valle; et al.; Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones; Elsevier Gmbh; Phytomedicine; 19; 13; 9-2012; 1173-1177
0944-7113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/77653
identifier_str_mv Amaya, Susana del Valle; Pereira, José A.; Borkosky, Susana Alicia; Valdéz, Juan Carlos; Bardon, Alicia del Valle; et al.; Inhibition of quorum sensing in pseudomonas aeruginosa by sesquiterpene lactones; Elsevier Gmbh; Phytomedicine; 19; 13; 9-2012; 1173-1177
0944-7113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2012.07.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0944711312002139?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269546721837056
score 13.13397

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