Levans production by Gluconacetobacter diazotrophicus

Autores
Molinari, Maria Laura; Boiardi, Jose Luis
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Growth of Gluconacetobacterdiazotrophicuswith glucose as carbon an energy source has been extensively studied. However, there are no reports in the literature describing growth of G.diazotrophicusin cultures containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. Biomass, levans, gluconic acid andketogluconic acids production and levansucrase activity were determined in cultures with different sucrose concentration and nitrogen sources. Results: The biomass production was maximal in cultures containing 100 gL-1 sucrose and inorganic nitrogen. Gluconic acid production was observed under all conditions tested, at levels up to 9 gL-1 in cultures with sucrose excess and biological N2-fixation (BNF). Ketogluconic acids were detectable only in cultures with sucrose excess and supplemented with organic nitrogen sources. Levans production, although observed in all cultures, was maximal in batch culture with 100 gL-1 of sucrose and BNF, concomitant with a significant expression of extracellular levansucrase. Conclusions: Ours results not only describe some unknown aspects of G. diazotrophicus physiology, but open up the possibility of developing a technology oflevans production by this organism using culture media with sucrose (or some cheaper substitute, like molasses) and without the addition of any N-source because of its ability of fixing atmospheric N2.
Fil: Molinari, Maria Laura. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - la Plata. Cent.de Inv En Fermentaciones Industriales (i); Argentina
Fil: Boiardi, Jose Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - la Plata. Cent.de Inv En Fermentaciones Industriales (i); Argentina
Materia
levans
fos
levansucrase
gluconacetobacter diazotrophicus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3389
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/3389
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Levans production by Gluconacetobacter diazotrophicusMolinari, Maria LauraBoiardi, Jose Luislevansfoslevansucrasegluconacetobacter diazotrophicushttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Background: Growth of Gluconacetobacterdiazotrophicuswith glucose as carbon an energy source has been extensively studied. However, there are no reports in the literature describing growth of G.diazotrophicusin cultures containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. Biomass, levans, gluconic acid andketogluconic acids production and levansucrase activity were determined in cultures with different sucrose concentration and nitrogen sources. Results: The biomass production was maximal in cultures containing 100 gL-1 sucrose and inorganic nitrogen. Gluconic acid production was observed under all conditions tested, at levels up to 9 gL-1 in cultures with sucrose excess and biological N2-fixation (BNF). Ketogluconic acids were detectable only in cultures with sucrose excess and supplemented with organic nitrogen sources. Levans production, although observed in all cultures, was maximal in batch culture with 100 gL-1 of sucrose and BNF, concomitant with a significant expression of extracellular levansucrase. Conclusions: Ours results not only describe some unknown aspects of G. diazotrophicus physiology, but open up the possibility of developing a technology oflevans production by this organism using culture media with sucrose (or some cheaper substitute, like molasses) and without the addition of any N-source because of its ability of fixing atmospheric N2.Fil: Molinari, Maria Laura. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - la Plata. Cent.de Inv En Fermentaciones Industriales (i); ArgentinaFil: Boiardi, Jose Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - la Plata. Cent.de Inv En Fermentaciones Industriales (i); ArgentinaUniv Catolica de Valparaiso2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3389Molinari, Maria Laura; Boiardi, Jose Luis; Levans production by Gluconacetobacter diazotrophicus; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 4-20130717-3458enginfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/9xprsqinfo:eu-repo/semantics/altIdentifier/doi/10.2225/vol16-issue3-fulltext-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:14Zoai:ri.conicet.gov.ar:11336/3389instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:14.536CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Levans production by Gluconacetobacter diazotrophicus
title Levans production by Gluconacetobacter diazotrophicus
spellingShingle Levans production by Gluconacetobacter diazotrophicus
Molinari, Maria Laura
levans
fos
levansucrase
gluconacetobacter diazotrophicus
title_short Levans production by Gluconacetobacter diazotrophicus
title_full Levans production by Gluconacetobacter diazotrophicus
title_fullStr Levans production by Gluconacetobacter diazotrophicus
title_full_unstemmed Levans production by Gluconacetobacter diazotrophicus
title_sort Levans production by Gluconacetobacter diazotrophicus
dc.creator.none.fl_str_mv Molinari, Maria Laura
Boiardi, Jose Luis
author Molinari, Maria Laura
author_facet Molinari, Maria Laura
Boiardi, Jose Luis
author_role author
author2 Boiardi, Jose Luis
author2_role author
dc.subject.none.fl_str_mv levans
fos
levansucrase
gluconacetobacter diazotrophicus
topic levans
fos
levansucrase
gluconacetobacter diazotrophicus
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Background: Growth of Gluconacetobacterdiazotrophicuswith glucose as carbon an energy source has been extensively studied. However, there are no reports in the literature describing growth of G.diazotrophicusin cultures containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. Biomass, levans, gluconic acid andketogluconic acids production and levansucrase activity were determined in cultures with different sucrose concentration and nitrogen sources. Results: The biomass production was maximal in cultures containing 100 gL-1 sucrose and inorganic nitrogen. Gluconic acid production was observed under all conditions tested, at levels up to 9 gL-1 in cultures with sucrose excess and biological N2-fixation (BNF). Ketogluconic acids were detectable only in cultures with sucrose excess and supplemented with organic nitrogen sources. Levans production, although observed in all cultures, was maximal in batch culture with 100 gL-1 of sucrose and BNF, concomitant with a significant expression of extracellular levansucrase. Conclusions: Ours results not only describe some unknown aspects of G. diazotrophicus physiology, but open up the possibility of developing a technology oflevans production by this organism using culture media with sucrose (or some cheaper substitute, like molasses) and without the addition of any N-source because of its ability of fixing atmospheric N2.
Fil: Molinari, Maria Laura. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - la Plata. Cent.de Inv En Fermentaciones Industriales (i); Argentina
Fil: Boiardi, Jose Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - la Plata. Cent.de Inv En Fermentaciones Industriales (i); Argentina
description Background: Growth of Gluconacetobacterdiazotrophicuswith glucose as carbon an energy source has been extensively studied. However, there are no reports in the literature describing growth of G.diazotrophicusin cultures containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. Biomass, levans, gluconic acid andketogluconic acids production and levansucrase activity were determined in cultures with different sucrose concentration and nitrogen sources. Results: The biomass production was maximal in cultures containing 100 gL-1 sucrose and inorganic nitrogen. Gluconic acid production was observed under all conditions tested, at levels up to 9 gL-1 in cultures with sucrose excess and biological N2-fixation (BNF). Ketogluconic acids were detectable only in cultures with sucrose excess and supplemented with organic nitrogen sources. Levans production, although observed in all cultures, was maximal in batch culture with 100 gL-1 of sucrose and BNF, concomitant with a significant expression of extracellular levansucrase. Conclusions: Ours results not only describe some unknown aspects of G. diazotrophicus physiology, but open up the possibility of developing a technology oflevans production by this organism using culture media with sucrose (or some cheaper substitute, like molasses) and without the addition of any N-source because of its ability of fixing atmospheric N2.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3389
Molinari, Maria Laura; Boiardi, Jose Luis; Levans production by Gluconacetobacter diazotrophicus; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 4-2013
0717-3458
url http://hdl.handle.net/11336/3389
identifier_str_mv Molinari, Maria Laura; Boiardi, Jose Luis; Levans production by Gluconacetobacter diazotrophicus; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 4-2013
0717-3458
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/9xprsq
info:eu-repo/semantics/altIdentifier/doi/10.2225/vol16-issue3-fulltext-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Univ Catolica de Valparaiso
publisher.none.fl_str_mv Univ Catolica de Valparaiso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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