Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance

Autores
Santiago Valtierra, Florencia Ximena; Luquez, Jessica Mariela; Tajes Ardanaz, Oliverio Julián; Torlaschi, Camila; Oresti, Gerardo Martin
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Spermatogenesis has been achieved in vitro using a gas-liquid interphase culture system. In this setting, it is possible to follow lipid metabolism to know its role during the spermatogenic process, thus gathering potentially useful information for ex vivo spermatogenesis biotechnology. We observed a relationship between the progression of spermatogenesis in both, in vivo and ex vivo, at cytological and histological level and the gene expression of enzymes involved in fatty acid desaturation, elongation and transport. The aim of this study was to examine and extend whether developmental changes that occur in vivo in testicular lipids with long-chain (C18- C22) and very long-chain (≥ C24) polyunsaturated fatty acids (PUFA) also occur ex vivo in explants of neonatal testes in culture. Explants from 6-day old mice cultured for 22 days showed that differentiation proceed from spermatogonial stem cells up to haploid round spermatids. Notably, after 44 days in culture we were able to detect some spermatozoa, although still scarce. Interestingly, like in vivo, total lipids from explants increased their proportion of PUFA during the period in culture. In addition to the common n-6 and n-3 PUFAs (22:5n-6, 22:6n-3, 24:4 and 24:5) we observed that unusual n9 PUFAs (20:3n-9 and 22:4n-9) were accumulated in the explants. The 22:5n-6/20:4n-6 ratio in the glycerophospholipids was increased during the first 22 days associated to the appearance of haploid spermatids and then it remains unchanged until day 44. In addition, we noted that in culture the testicular tissue accumulated neutral lipids, mainly, triacylglycerides (TAG) and cholesterol esters (CE) that contained a high proportion of n-9 PUFAs. In vivo, the increase of neutral lipids occurs in testicular tissue associated with spermatogenesis impairment. The presence of the unusual fatty acids of the n-9 serie in the explants suggests that the culture system does not ensure the provision of essential fatty acids to the tissue. Addressing this point could improve the rate of gamete production in this system
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Tajes Ardanaz, Oliverio Julián. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Torlaschi, Camila. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
LIPIDS
SPERMATOGENESIS
EX VIVO
n-9 PUFA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230875
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/230875
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenanceSantiago Valtierra, Florencia XimenaLuquez, Jessica MarielaTajes Ardanaz, Oliverio JuliánTorlaschi, CamilaOresti, Gerardo MartinLIPIDSSPERMATOGENESISEX VIVOn-9 PUFAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Spermatogenesis has been achieved in vitro using a gas-liquid interphase culture system. In this setting, it is possible to follow lipid metabolism to know its role during the spermatogenic process, thus gathering potentially useful information for ex vivo spermatogenesis biotechnology. We observed a relationship between the progression of spermatogenesis in both, in vivo and ex vivo, at cytological and histological level and the gene expression of enzymes involved in fatty acid desaturation, elongation and transport. The aim of this study was to examine and extend whether developmental changes that occur in vivo in testicular lipids with long-chain (C18- C22) and very long-chain (≥ C24) polyunsaturated fatty acids (PUFA) also occur ex vivo in explants of neonatal testes in culture. Explants from 6-day old mice cultured for 22 days showed that differentiation proceed from spermatogonial stem cells up to haploid round spermatids. Notably, after 44 days in culture we were able to detect some spermatozoa, although still scarce. Interestingly, like in vivo, total lipids from explants increased their proportion of PUFA during the period in culture. In addition to the common n-6 and n-3 PUFAs (22:5n-6, 22:6n-3, 24:4 and 24:5) we observed that unusual n9 PUFAs (20:3n-9 and 22:4n-9) were accumulated in the explants. The 22:5n-6/20:4n-6 ratio in the glycerophospholipids was increased during the first 22 days associated to the appearance of haploid spermatids and then it remains unchanged until day 44. In addition, we noted that in culture the testicular tissue accumulated neutral lipids, mainly, triacylglycerides (TAG) and cholesterol esters (CE) that contained a high proportion of n-9 PUFAs. In vivo, the increase of neutral lipids occurs in testicular tissue associated with spermatogenesis impairment. The presence of the unusual fatty acids of the n-9 serie in the explants suggests that the culture system does not ensure the provision of essential fatty acids to the tissue. Addressing this point could improve the rate of gamete production in this systemFil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Tajes Ardanaz, Oliverio Julián. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Torlaschi, Camila. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology ResearchMendozaArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230875Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 176-1760327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.1/50703/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:42Zoai:ri.conicet.gov.ar:11336/230875instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:43.115CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance
title Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance
spellingShingle Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance
Santiago Valtierra, Florencia Ximena
LIPIDS
SPERMATOGENESIS
EX VIVO
n-9 PUFA
title_short Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance
title_full Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance
title_fullStr Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance
title_full_unstemmed Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance
title_sort Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance
dc.creator.none.fl_str_mv Santiago Valtierra, Florencia Ximena
Luquez, Jessica Mariela
Tajes Ardanaz, Oliverio Julián
Torlaschi, Camila
Oresti, Gerardo Martin
author Santiago Valtierra, Florencia Ximena
author_facet Santiago Valtierra, Florencia Ximena
Luquez, Jessica Mariela
Tajes Ardanaz, Oliverio Julián
Torlaschi, Camila
Oresti, Gerardo Martin
author_role author
author2 Luquez, Jessica Mariela
Tajes Ardanaz, Oliverio Julián
Torlaschi, Camila
Oresti, Gerardo Martin
author2_role author
author
author
author
dc.subject.none.fl_str_mv LIPIDS
SPERMATOGENESIS
EX VIVO
n-9 PUFA
topic LIPIDS
SPERMATOGENESIS
EX VIVO
n-9 PUFA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Spermatogenesis has been achieved in vitro using a gas-liquid interphase culture system. In this setting, it is possible to follow lipid metabolism to know its role during the spermatogenic process, thus gathering potentially useful information for ex vivo spermatogenesis biotechnology. We observed a relationship between the progression of spermatogenesis in both, in vivo and ex vivo, at cytological and histological level and the gene expression of enzymes involved in fatty acid desaturation, elongation and transport. The aim of this study was to examine and extend whether developmental changes that occur in vivo in testicular lipids with long-chain (C18- C22) and very long-chain (≥ C24) polyunsaturated fatty acids (PUFA) also occur ex vivo in explants of neonatal testes in culture. Explants from 6-day old mice cultured for 22 days showed that differentiation proceed from spermatogonial stem cells up to haploid round spermatids. Notably, after 44 days in culture we were able to detect some spermatozoa, although still scarce. Interestingly, like in vivo, total lipids from explants increased their proportion of PUFA during the period in culture. In addition to the common n-6 and n-3 PUFAs (22:5n-6, 22:6n-3, 24:4 and 24:5) we observed that unusual n9 PUFAs (20:3n-9 and 22:4n-9) were accumulated in the explants. The 22:5n-6/20:4n-6 ratio in the glycerophospholipids was increased during the first 22 days associated to the appearance of haploid spermatids and then it remains unchanged until day 44. In addition, we noted that in culture the testicular tissue accumulated neutral lipids, mainly, triacylglycerides (TAG) and cholesterol esters (CE) that contained a high proportion of n-9 PUFAs. In vivo, the increase of neutral lipids occurs in testicular tissue associated with spermatogenesis impairment. The presence of the unusual fatty acids of the n-9 serie in the explants suggests that the culture system does not ensure the provision of essential fatty acids to the tissue. Addressing this point could improve the rate of gamete production in this system
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Tajes Ardanaz, Oliverio Julián. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Torlaschi, Camila. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Spermatogenesis has been achieved in vitro using a gas-liquid interphase culture system. In this setting, it is possible to follow lipid metabolism to know its role during the spermatogenic process, thus gathering potentially useful information for ex vivo spermatogenesis biotechnology. We observed a relationship between the progression of spermatogenesis in both, in vivo and ex vivo, at cytological and histological level and the gene expression of enzymes involved in fatty acid desaturation, elongation and transport. The aim of this study was to examine and extend whether developmental changes that occur in vivo in testicular lipids with long-chain (C18- C22) and very long-chain (≥ C24) polyunsaturated fatty acids (PUFA) also occur ex vivo in explants of neonatal testes in culture. Explants from 6-day old mice cultured for 22 days showed that differentiation proceed from spermatogonial stem cells up to haploid round spermatids. Notably, after 44 days in culture we were able to detect some spermatozoa, although still scarce. Interestingly, like in vivo, total lipids from explants increased their proportion of PUFA during the period in culture. In addition to the common n-6 and n-3 PUFAs (22:5n-6, 22:6n-3, 24:4 and 24:5) we observed that unusual n9 PUFAs (20:3n-9 and 22:4n-9) were accumulated in the explants. The 22:5n-6/20:4n-6 ratio in the glycerophospholipids was increased during the first 22 days associated to the appearance of haploid spermatids and then it remains unchanged until day 44. In addition, we noted that in culture the testicular tissue accumulated neutral lipids, mainly, triacylglycerides (TAG) and cholesterol esters (CE) that contained a high proportion of n-9 PUFAs. In vivo, the increase of neutral lipids occurs in testicular tissue associated with spermatogenesis impairment. The presence of the unusual fatty acids of the n-9 serie in the explants suggests that the culture system does not ensure the provision of essential fatty acids to the tissue. Addressing this point could improve the rate of gamete production in this system
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230875
Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 176-176
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230875
identifier_str_mv Progressive accumulation of n-9 PUFAs in testicular lipids during ex vivo tissue maintenance; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 176-176
0327-9545
1667-5746
CONICET Digital
CONICET
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.1/50703/pdf
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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