Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance

Autores
Sanchez Chaves, Manuela; Luquez, Jessica Mariela; Tajes Ardanaz, Oliverio; Oresti, Gerardo Martin
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In vitro spermatogenesis has been successfully achieved by utilizing a gas-liquid interphase culture system. Within this experimental framework, monitoring of lipid metabolism provides a deeper understanding of its significance within the spermatogenic process, thereby yielding valuable insights that could be applied to ex vivo spermatogenesis biotechnology. It is known that retinoic acid (RA) is required for spermatogonia differentiation, to complete the meiotic divisions in spermatocytes and to support the full development of spermatogenic cells into elongated spermatids. The molecular mechanism by which RA enhances the spermatogenesis process remains in part unknown. Here, we evaluated the effects of RA supplementation on testicular lipids with long-chain (C18-C22) polyunsaturated fatty acids (PUFA) during ex vivo spermatogenesis progression in prepuberal mouse testicular explants. An analysis of spermatogenic cell types in testis explants from 6-day-old mice cultured for 22 days revealed progress in the differentiation of spermatogonial stem cells to haploid round spermatids. After 44 days in culture, some sperm were detected. Notably, the incidence of spermatozoa per unit of tissue was higher in the presence of RA than in its absence. In addition, the explants exposed to RA had lower amounts of neutral lipids, especially triacylglycerols, than those cultured in basal medium. Moreover, the addition of RA led to a decrease in the proportion of uncommon PUFAs (20:3n-9 and 22:4n-9), which we had previously observed as accumulating in neutral lipids from explants cultured under basal conditions. Simultaneously, similar to what occurs in vivo, membrane glycerophospholipids and cholesterol esters increased their proportion of C20-C22 n-6 PUFA. These lipids changes were linked to an augmentation in testosterone production and an increase in the haploid cell in the explants supplemented with RA. The similarity between these lipid changes and those observed in vivo during normal testicular posnatal development underscores the pivotal role of RA in optimizing ex vivo spermatogenesis.
Fil: Sanchez Chaves, Manuela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Luquez, Jessica Mariela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Tajes Ardanaz, Oliverio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
PUFA
PHOSPHOLIPIDS
NEUTRAL LIPIDS
SPERMATOGENESIS
RETINOID ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232550
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/232550
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenanceSanchez Chaves, ManuelaLuquez, Jessica MarielaTajes Ardanaz, OliverioOresti, Gerardo MartinPUFAPHOSPHOLIPIDSNEUTRAL LIPIDSSPERMATOGENESISRETINOID ACIDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In vitro spermatogenesis has been successfully achieved by utilizing a gas-liquid interphase culture system. Within this experimental framework, monitoring of lipid metabolism provides a deeper understanding of its significance within the spermatogenic process, thereby yielding valuable insights that could be applied to ex vivo spermatogenesis biotechnology. It is known that retinoic acid (RA) is required for spermatogonia differentiation, to complete the meiotic divisions in spermatocytes and to support the full development of spermatogenic cells into elongated spermatids. The molecular mechanism by which RA enhances the spermatogenesis process remains in part unknown. Here, we evaluated the effects of RA supplementation on testicular lipids with long-chain (C18-C22) polyunsaturated fatty acids (PUFA) during ex vivo spermatogenesis progression in prepuberal mouse testicular explants. An analysis of spermatogenic cell types in testis explants from 6-day-old mice cultured for 22 days revealed progress in the differentiation of spermatogonial stem cells to haploid round spermatids. After 44 days in culture, some sperm were detected. Notably, the incidence of spermatozoa per unit of tissue was higher in the presence of RA than in its absence. In addition, the explants exposed to RA had lower amounts of neutral lipids, especially triacylglycerols, than those cultured in basal medium. Moreover, the addition of RA led to a decrease in the proportion of uncommon PUFAs (20:3n-9 and 22:4n-9), which we had previously observed as accumulating in neutral lipids from explants cultured under basal conditions. Simultaneously, similar to what occurs in vivo, membrane glycerophospholipids and cholesterol esters increased their proportion of C20-C22 n-6 PUFA. These lipids changes were linked to an augmentation in testosterone production and an increase in the haploid cell in the explants supplemented with RA. The similarity between these lipid changes and those observed in vivo during normal testicular posnatal development underscores the pivotal role of RA in optimizing ex vivo spermatogenesis.Fil: Sanchez Chaves, Manuela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Luquez, Jessica Mariela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Tajes Ardanaz, Oliverio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaReunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularRosarioArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Investigación en Bioquímica y Biología Molecular2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232550Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance; Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Rosario; Argentina; 2023; 233-234CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2023/abstracts-EN.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:46Zoai:ri.conicet.gov.ar:11336/232550instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:47.083CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance
title Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance
spellingShingle Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance
Sanchez Chaves, Manuela
PUFA
PHOSPHOLIPIDS
NEUTRAL LIPIDS
SPERMATOGENESIS
RETINOID ACID
title_short Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance
title_full Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance
title_fullStr Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance
title_full_unstemmed Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance
title_sort Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance
dc.creator.none.fl_str_mv Sanchez Chaves, Manuela
Luquez, Jessica Mariela
Tajes Ardanaz, Oliverio
Oresti, Gerardo Martin
author Sanchez Chaves, Manuela
author_facet Sanchez Chaves, Manuela
Luquez, Jessica Mariela
Tajes Ardanaz, Oliverio
Oresti, Gerardo Martin
author_role author
author2 Luquez, Jessica Mariela
Tajes Ardanaz, Oliverio
Oresti, Gerardo Martin
author2_role author
author
author
dc.subject.none.fl_str_mv PUFA
PHOSPHOLIPIDS
NEUTRAL LIPIDS
SPERMATOGENESIS
RETINOID ACID
topic PUFA
PHOSPHOLIPIDS
NEUTRAL LIPIDS
SPERMATOGENESIS
RETINOID ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In vitro spermatogenesis has been successfully achieved by utilizing a gas-liquid interphase culture system. Within this experimental framework, monitoring of lipid metabolism provides a deeper understanding of its significance within the spermatogenic process, thereby yielding valuable insights that could be applied to ex vivo spermatogenesis biotechnology. It is known that retinoic acid (RA) is required for spermatogonia differentiation, to complete the meiotic divisions in spermatocytes and to support the full development of spermatogenic cells into elongated spermatids. The molecular mechanism by which RA enhances the spermatogenesis process remains in part unknown. Here, we evaluated the effects of RA supplementation on testicular lipids with long-chain (C18-C22) polyunsaturated fatty acids (PUFA) during ex vivo spermatogenesis progression in prepuberal mouse testicular explants. An analysis of spermatogenic cell types in testis explants from 6-day-old mice cultured for 22 days revealed progress in the differentiation of spermatogonial stem cells to haploid round spermatids. After 44 days in culture, some sperm were detected. Notably, the incidence of spermatozoa per unit of tissue was higher in the presence of RA than in its absence. In addition, the explants exposed to RA had lower amounts of neutral lipids, especially triacylglycerols, than those cultured in basal medium. Moreover, the addition of RA led to a decrease in the proportion of uncommon PUFAs (20:3n-9 and 22:4n-9), which we had previously observed as accumulating in neutral lipids from explants cultured under basal conditions. Simultaneously, similar to what occurs in vivo, membrane glycerophospholipids and cholesterol esters increased their proportion of C20-C22 n-6 PUFA. These lipids changes were linked to an augmentation in testosterone production and an increase in the haploid cell in the explants supplemented with RA. The similarity between these lipid changes and those observed in vivo during normal testicular posnatal development underscores the pivotal role of RA in optimizing ex vivo spermatogenesis.
Fil: Sanchez Chaves, Manuela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Luquez, Jessica Mariela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Tajes Ardanaz, Oliverio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description In vitro spermatogenesis has been successfully achieved by utilizing a gas-liquid interphase culture system. Within this experimental framework, monitoring of lipid metabolism provides a deeper understanding of its significance within the spermatogenic process, thereby yielding valuable insights that could be applied to ex vivo spermatogenesis biotechnology. It is known that retinoic acid (RA) is required for spermatogonia differentiation, to complete the meiotic divisions in spermatocytes and to support the full development of spermatogenic cells into elongated spermatids. The molecular mechanism by which RA enhances the spermatogenesis process remains in part unknown. Here, we evaluated the effects of RA supplementation on testicular lipids with long-chain (C18-C22) polyunsaturated fatty acids (PUFA) during ex vivo spermatogenesis progression in prepuberal mouse testicular explants. An analysis of spermatogenic cell types in testis explants from 6-day-old mice cultured for 22 days revealed progress in the differentiation of spermatogonial stem cells to haploid round spermatids. After 44 days in culture, some sperm were detected. Notably, the incidence of spermatozoa per unit of tissue was higher in the presence of RA than in its absence. In addition, the explants exposed to RA had lower amounts of neutral lipids, especially triacylglycerols, than those cultured in basal medium. Moreover, the addition of RA led to a decrease in the proportion of uncommon PUFAs (20:3n-9 and 22:4n-9), which we had previously observed as accumulating in neutral lipids from explants cultured under basal conditions. Simultaneously, similar to what occurs in vivo, membrane glycerophospholipids and cholesterol esters increased their proportion of C20-C22 n-6 PUFA. These lipids changes were linked to an augmentation in testosterone production and an increase in the haploid cell in the explants supplemented with RA. The similarity between these lipid changes and those observed in vivo during normal testicular posnatal development underscores the pivotal role of RA in optimizing ex vivo spermatogenesis.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232550
Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance; Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Rosario; Argentina; 2023; 233-234
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232550
identifier_str_mv Effects of retinoic acid on testicular lipids with polyunsaturated fatty acid during ex vivo tissue maintenance; Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Rosario; Argentina; 2023; 233-234
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2023/abstracts-EN.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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