Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP

Autores
Almeyda, María Delfina; Scodelaro Bilbao, Paola Gabriela; Constenla, Diana Teresita; Leonardi, Patricia Ines
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The nutritional value of cryptophytes is of great importance due to the presence of high amounts of polyunsaturated fatty acids (PUFAs), sterols and amino acids. Therefore, native cryptophytes have biotechnological potential as a source of high-value products for nutraceutical and aquaculture industries. The synthesis of these metabolites is conditioned by both the strain and the cultivation conditions, being temperature one of the main factors for PUFAs synthesis. The objective of this work was to evaluate the effect of low temperature stress on the production of PUFAs and sterols in the marine cryptophyte Plagioselmis sp. cultivated in a photobioreactor. Plagioselmis sp. was isolated from Bahía Blanca?s Estuary. Cultures were carried out for 10 days under two temperature conditions: 1) continuously at 20°C (Control) and 2) lowered to 11°C during the stationary growth phase (Low Temperature Stress, LTS). TAG and sterols were separated through thin layer chromatography (TLC) and quantified spectrophotometrically. Lipid extraction and fractionation into neutral lipids (NL), glycolipids (GL) and phospholipids (PL) were performed. These fractions were analyzed by gas chromatography. LTS significantly increased lipid production by ≈40%. Both temperature conditions showed TAG and sterol accumulation within the days of cultivation. NL was the main lipid fraction (≈63% of Total Lipids, TL) followed by GL (≈32% of TL) and PL (≈5% of TL) for both temperature conditions. PUFA content (expressed as % of total FAME) was significantly higher in the LTS condition (41.3%) than in the control (35.71%), mainly due to PUFAs from the NL fraction. Omega-3 fatty acids (ω-3 FAs) represented 19.62% of the Control and 22.72% of the LTS condition, while ω-6 FAs comprised 16.09% (Control) and 18.65% (SLT). The most abundant PUFAs were eicosapentaenoic (EPA) and docosapentaenoic (DPA), which significantly increased due to LTS from 14.6% to 18.17% (EPA) and from 5.35 to 9.29% (DPA). Under LTS the production of PUFAs was of 13.5 mg L-1 being 7.41 mg L-1 ω-3 FAs and 6.09 mg L-1 ω-6 FAs. The production of EPA and DPA FAs was 5.93 mg L-1 and 3.03 mg L-1, respectively. The results point out the potential of the native microalga Plagioselmis sp. to develop a sustainable biotechnological system for the production of PUFAs with nutraceutical and aquaculture applications.
Fil: Almeyda, María Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Scodelaro Bilbao, Paola Gabriela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química; Argentina
Fil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
LVII SAIB Meeting; XVI SAMIGE Meeting
Buenos Aires
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
MICROALGAS
PUFA
BIOACTIVOS
ALIMENTOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189295
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/189295
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SPAlmeyda, María DelfinaScodelaro Bilbao, Paola GabrielaConstenla, Diana TeresitaLeonardi, Patricia InesMICROALGASPUFABIOACTIVOSALIMENTOShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The nutritional value of cryptophytes is of great importance due to the presence of high amounts of polyunsaturated fatty acids (PUFAs), sterols and amino acids. Therefore, native cryptophytes have biotechnological potential as a source of high-value products for nutraceutical and aquaculture industries. The synthesis of these metabolites is conditioned by both the strain and the cultivation conditions, being temperature one of the main factors for PUFAs synthesis. The objective of this work was to evaluate the effect of low temperature stress on the production of PUFAs and sterols in the marine cryptophyte Plagioselmis sp. cultivated in a photobioreactor. Plagioselmis sp. was isolated from Bahía Blanca?s Estuary. Cultures were carried out for 10 days under two temperature conditions: 1) continuously at 20°C (Control) and 2) lowered to 11°C during the stationary growth phase (Low Temperature Stress, LTS). TAG and sterols were separated through thin layer chromatography (TLC) and quantified spectrophotometrically. Lipid extraction and fractionation into neutral lipids (NL), glycolipids (GL) and phospholipids (PL) were performed. These fractions were analyzed by gas chromatography. LTS significantly increased lipid production by ≈40%. Both temperature conditions showed TAG and sterol accumulation within the days of cultivation. NL was the main lipid fraction (≈63% of Total Lipids, TL) followed by GL (≈32% of TL) and PL (≈5% of TL) for both temperature conditions. PUFA content (expressed as % of total FAME) was significantly higher in the LTS condition (41.3%) than in the control (35.71%), mainly due to PUFAs from the NL fraction. Omega-3 fatty acids (ω-3 FAs) represented 19.62% of the Control and 22.72% of the LTS condition, while ω-6 FAs comprised 16.09% (Control) and 18.65% (SLT). The most abundant PUFAs were eicosapentaenoic (EPA) and docosapentaenoic (DPA), which significantly increased due to LTS from 14.6% to 18.17% (EPA) and from 5.35 to 9.29% (DPA). Under LTS the production of PUFAs was of 13.5 mg L-1 being 7.41 mg L-1 ω-3 FAs and 6.09 mg L-1 ω-6 FAs. The production of EPA and DPA FAs was 5.93 mg L-1 and 3.03 mg L-1, respectively. The results point out the potential of the native microalga Plagioselmis sp. to develop a sustainable biotechnological system for the production of PUFAs with nutraceutical and aquaculture applications.Fil: Almeyda, María Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Scodelaro Bilbao, Paola Gabriela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química; ArgentinaFil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaLVII SAIB Meeting; XVI SAMIGE MeetingBuenos AiresArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189295Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP; LVII SAIB Meeting; XVI SAMIGE Meeting; Buenos Aires; Argentina; 2021; 1-20327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://congresos.g2consultora.com/wp-content/uploads/2021/10/Biocell-Preprint-SAIB-SAMIGE-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:42:08Zoai:ri.conicet.gov.ar:11336/189295instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:42:08.32CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP
title Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP
spellingShingle Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP
Almeyda, María Delfina
MICROALGAS
PUFA
BIOACTIVOS
ALIMENTOS
title_short Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP
title_full Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP
title_fullStr Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP
title_full_unstemmed Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP
title_sort Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP
dc.creator.none.fl_str_mv Almeyda, María Delfina
Scodelaro Bilbao, Paola Gabriela
Constenla, Diana Teresita
Leonardi, Patricia Ines
author Almeyda, María Delfina
author_facet Almeyda, María Delfina
Scodelaro Bilbao, Paola Gabriela
Constenla, Diana Teresita
Leonardi, Patricia Ines
author_role author
author2 Scodelaro Bilbao, Paola Gabriela
Constenla, Diana Teresita
Leonardi, Patricia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv MICROALGAS
PUFA
BIOACTIVOS
ALIMENTOS
topic MICROALGAS
PUFA
BIOACTIVOS
ALIMENTOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The nutritional value of cryptophytes is of great importance due to the presence of high amounts of polyunsaturated fatty acids (PUFAs), sterols and amino acids. Therefore, native cryptophytes have biotechnological potential as a source of high-value products for nutraceutical and aquaculture industries. The synthesis of these metabolites is conditioned by both the strain and the cultivation conditions, being temperature one of the main factors for PUFAs synthesis. The objective of this work was to evaluate the effect of low temperature stress on the production of PUFAs and sterols in the marine cryptophyte Plagioselmis sp. cultivated in a photobioreactor. Plagioselmis sp. was isolated from Bahía Blanca?s Estuary. Cultures were carried out for 10 days under two temperature conditions: 1) continuously at 20°C (Control) and 2) lowered to 11°C during the stationary growth phase (Low Temperature Stress, LTS). TAG and sterols were separated through thin layer chromatography (TLC) and quantified spectrophotometrically. Lipid extraction and fractionation into neutral lipids (NL), glycolipids (GL) and phospholipids (PL) were performed. These fractions were analyzed by gas chromatography. LTS significantly increased lipid production by ≈40%. Both temperature conditions showed TAG and sterol accumulation within the days of cultivation. NL was the main lipid fraction (≈63% of Total Lipids, TL) followed by GL (≈32% of TL) and PL (≈5% of TL) for both temperature conditions. PUFA content (expressed as % of total FAME) was significantly higher in the LTS condition (41.3%) than in the control (35.71%), mainly due to PUFAs from the NL fraction. Omega-3 fatty acids (ω-3 FAs) represented 19.62% of the Control and 22.72% of the LTS condition, while ω-6 FAs comprised 16.09% (Control) and 18.65% (SLT). The most abundant PUFAs were eicosapentaenoic (EPA) and docosapentaenoic (DPA), which significantly increased due to LTS from 14.6% to 18.17% (EPA) and from 5.35 to 9.29% (DPA). Under LTS the production of PUFAs was of 13.5 mg L-1 being 7.41 mg L-1 ω-3 FAs and 6.09 mg L-1 ω-6 FAs. The production of EPA and DPA FAs was 5.93 mg L-1 and 3.03 mg L-1, respectively. The results point out the potential of the native microalga Plagioselmis sp. to develop a sustainable biotechnological system for the production of PUFAs with nutraceutical and aquaculture applications.
Fil: Almeyda, María Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Scodelaro Bilbao, Paola Gabriela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química; Argentina
Fil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
LVII SAIB Meeting; XVI SAMIGE Meeting
Buenos Aires
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description The nutritional value of cryptophytes is of great importance due to the presence of high amounts of polyunsaturated fatty acids (PUFAs), sterols and amino acids. Therefore, native cryptophytes have biotechnological potential as a source of high-value products for nutraceutical and aquaculture industries. The synthesis of these metabolites is conditioned by both the strain and the cultivation conditions, being temperature one of the main factors for PUFAs synthesis. The objective of this work was to evaluate the effect of low temperature stress on the production of PUFAs and sterols in the marine cryptophyte Plagioselmis sp. cultivated in a photobioreactor. Plagioselmis sp. was isolated from Bahía Blanca?s Estuary. Cultures were carried out for 10 days under two temperature conditions: 1) continuously at 20°C (Control) and 2) lowered to 11°C during the stationary growth phase (Low Temperature Stress, LTS). TAG and sterols were separated through thin layer chromatography (TLC) and quantified spectrophotometrically. Lipid extraction and fractionation into neutral lipids (NL), glycolipids (GL) and phospholipids (PL) were performed. These fractions were analyzed by gas chromatography. LTS significantly increased lipid production by ≈40%. Both temperature conditions showed TAG and sterol accumulation within the days of cultivation. NL was the main lipid fraction (≈63% of Total Lipids, TL) followed by GL (≈32% of TL) and PL (≈5% of TL) for both temperature conditions. PUFA content (expressed as % of total FAME) was significantly higher in the LTS condition (41.3%) than in the control (35.71%), mainly due to PUFAs from the NL fraction. Omega-3 fatty acids (ω-3 FAs) represented 19.62% of the Control and 22.72% of the LTS condition, while ω-6 FAs comprised 16.09% (Control) and 18.65% (SLT). The most abundant PUFAs were eicosapentaenoic (EPA) and docosapentaenoic (DPA), which significantly increased due to LTS from 14.6% to 18.17% (EPA) and from 5.35 to 9.29% (DPA). Under LTS the production of PUFAs was of 13.5 mg L-1 being 7.41 mg L-1 ω-3 FAs and 6.09 mg L-1 ω-6 FAs. The production of EPA and DPA FAs was 5.93 mg L-1 and 3.03 mg L-1, respectively. The results point out the potential of the native microalga Plagioselmis sp. to develop a sustainable biotechnological system for the production of PUFAs with nutraceutical and aquaculture applications.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189295
Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP; LVII SAIB Meeting; XVI SAMIGE Meeting; Buenos Aires; Argentina; 2021; 1-2
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189295
identifier_str_mv Low Temperatura Induces PUFAS Production in a Native . Microalga Cryptophyte Plagioselmis SP; LVII SAIB Meeting; XVI SAMIGE Meeting; Buenos Aires; Argentina; 2021; 1-2
0327-9545
CONICET Digital
CONICET
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language eng
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dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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