Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis

Autores
Luquez, Jessica Mariela; Santiago Valtierra, Florencia Ximena; Isoler Alcaraz, Javier; Aveldaño, Marta Isabel; del Mazo, Jesús; Oresti, Gerardo Martin
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Using a gas-liquid interphase culture system from neonatal mouse testes, we previously observed in ex vivo explants a relationship between the progression of spermatogenesis at both cytological and histological levels and the gene expression of some of the enzymes involved in lipid metabolism. Here, we examined by RT-qPCR the expression of two PUFA elongases (Elovl2 and Elovl4), Δ6-desaturase (Fads2), fatty acid 2- hydroxylase (Fa2h), two fatty-acid-binding proteins (Fabp3 and Fabp9) and a diacylglycerol acyltransferase (Dgat2). Testis explants from 6 days old mice cultured for 22 days evidenced progress in spermatogenesis beyond the meiotic phase in some of the tubules. Although delayed in vitro in comparison with the in vivo development, in both cases the appearance of haploid germ cells occurred concomitantly with an increase in the expression of Fabp9, Dgat2, and Fa2h. Interestingly, the genes involved in PUFA synthesis (Elovl2, Elovl4, Fads2) and transport (Fabp3) were up-regulated in the testicular explants in comparison with the in vivo situation. This suggested, as a possible cause, partial insufficiency in the culture system of the C20-C22 PUFA required as substrates by these biosynthetic enzymes. This proved to be the case, as this medium contained low proportions (less than 4%) of these fatty acids. Supplementation of ex vivo explants with a PUFA-rich total lipid extract (TL) from adult mouse testis allowed progression into meiosis at the times in culture examined. Moreover, after 22 days in culture, the TL-supplemented explants contained more tubules with spermatogenic cells that had succeeded to reach the spermatid stage. Thus, in addition to growth factors and hormones, influences that promote the biosynthesis of PUFA-containing lipids are among the factors required to optimize spermatogenesis in ex vivo tissue explants
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Isoler Alcaraz, Javier. Consejo Superior de Investigaciones Científicas; España
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: del Mazo, Jesús. Consejo Superior de Investigaciones Científicas; España
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVI Reunión de la Sociedad. Argentina de Investigación en Bioquímica y Biología Molecular y XIV Congreso de la Pan American Association of Biochemistry & Molecular Biology Societies
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Panamerican Association for Biochemistry and Molecular Biology
Materia
ELONGASES
DESATURASES
FATTY ACID BINDING PROTEINS
SPERMATOGENESIS
PUFA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/217679
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesisLuquez, Jessica MarielaSantiago Valtierra, Florencia XimenaIsoler Alcaraz, JavierAveldaño, Marta Isabeldel Mazo, JesúsOresti, Gerardo MartinELONGASESDESATURASESFATTY ACID BINDING PROTEINSSPERMATOGENESISPUFAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Using a gas-liquid interphase culture system from neonatal mouse testes, we previously observed in ex vivo explants a relationship between the progression of spermatogenesis at both cytological and histological levels and the gene expression of some of the enzymes involved in lipid metabolism. Here, we examined by RT-qPCR the expression of two PUFA elongases (Elovl2 and Elovl4), Δ6-desaturase (Fads2), fatty acid 2- hydroxylase (Fa2h), two fatty-acid-binding proteins (Fabp3 and Fabp9) and a diacylglycerol acyltransferase (Dgat2). Testis explants from 6 days old mice cultured for 22 days evidenced progress in spermatogenesis beyond the meiotic phase in some of the tubules. Although delayed in vitro in comparison with the in vivo development, in both cases the appearance of haploid germ cells occurred concomitantly with an increase in the expression of Fabp9, Dgat2, and Fa2h. Interestingly, the genes involved in PUFA synthesis (Elovl2, Elovl4, Fads2) and transport (Fabp3) were up-regulated in the testicular explants in comparison with the in vivo situation. This suggested, as a possible cause, partial insufficiency in the culture system of the C20-C22 PUFA required as substrates by these biosynthetic enzymes. This proved to be the case, as this medium contained low proportions (less than 4%) of these fatty acids. Supplementation of ex vivo explants with a PUFA-rich total lipid extract (TL) from adult mouse testis allowed progression into meiosis at the times in culture examined. Moreover, after 22 days in culture, the TL-supplemented explants contained more tubules with spermatogenic cells that had succeeded to reach the spermatid stage. Thus, in addition to growth factors and hormones, influences that promote the biosynthesis of PUFA-containing lipids are among the factors required to optimize spermatogenesis in ex vivo tissue explantsFil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Isoler Alcaraz, Javier. Consejo Superior de Investigaciones Científicas; EspañaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: del Mazo, Jesús. Consejo Superior de Investigaciones Científicas; EspañaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLVI Reunión de la Sociedad. Argentina de Investigación en Bioquímica y Biología Molecular y XIV Congreso de la Pan American Association of Biochemistry & Molecular Biology SocietiesSaltaArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularPanamerican Association for Biochemistry and Molecular BiologyTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217679Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis; LVI Reunión de la Sociedad. Argentina de Investigación en Bioquímica y Biología Molecular y XIV Congreso de la Pan American Association of Biochemistry & Molecular Biology Societies; Salta; Argentina; 2019; 98-981667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.5/33868/pdfinfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.5Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:18Zoai:ri.conicet.gov.ar:11336/217679instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:19.074CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis
title Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis
spellingShingle Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis
Luquez, Jessica Mariela
ELONGASES
DESATURASES
FATTY ACID BINDING PROTEINS
SPERMATOGENESIS
PUFA
title_short Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis
title_full Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis
title_fullStr Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis
title_full_unstemmed Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis
title_sort Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis
dc.creator.none.fl_str_mv Luquez, Jessica Mariela
Santiago Valtierra, Florencia Ximena
Isoler Alcaraz, Javier
Aveldaño, Marta Isabel
del Mazo, Jesús
Oresti, Gerardo Martin
author Luquez, Jessica Mariela
author_facet Luquez, Jessica Mariela
Santiago Valtierra, Florencia Ximena
Isoler Alcaraz, Javier
Aveldaño, Marta Isabel
del Mazo, Jesús
Oresti, Gerardo Martin
author_role author
author2 Santiago Valtierra, Florencia Ximena
Isoler Alcaraz, Javier
Aveldaño, Marta Isabel
del Mazo, Jesús
Oresti, Gerardo Martin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ELONGASES
DESATURASES
FATTY ACID BINDING PROTEINS
SPERMATOGENESIS
PUFA
topic ELONGASES
DESATURASES
FATTY ACID BINDING PROTEINS
SPERMATOGENESIS
PUFA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Using a gas-liquid interphase culture system from neonatal mouse testes, we previously observed in ex vivo explants a relationship between the progression of spermatogenesis at both cytological and histological levels and the gene expression of some of the enzymes involved in lipid metabolism. Here, we examined by RT-qPCR the expression of two PUFA elongases (Elovl2 and Elovl4), Δ6-desaturase (Fads2), fatty acid 2- hydroxylase (Fa2h), two fatty-acid-binding proteins (Fabp3 and Fabp9) and a diacylglycerol acyltransferase (Dgat2). Testis explants from 6 days old mice cultured for 22 days evidenced progress in spermatogenesis beyond the meiotic phase in some of the tubules. Although delayed in vitro in comparison with the in vivo development, in both cases the appearance of haploid germ cells occurred concomitantly with an increase in the expression of Fabp9, Dgat2, and Fa2h. Interestingly, the genes involved in PUFA synthesis (Elovl2, Elovl4, Fads2) and transport (Fabp3) were up-regulated in the testicular explants in comparison with the in vivo situation. This suggested, as a possible cause, partial insufficiency in the culture system of the C20-C22 PUFA required as substrates by these biosynthetic enzymes. This proved to be the case, as this medium contained low proportions (less than 4%) of these fatty acids. Supplementation of ex vivo explants with a PUFA-rich total lipid extract (TL) from adult mouse testis allowed progression into meiosis at the times in culture examined. Moreover, after 22 days in culture, the TL-supplemented explants contained more tubules with spermatogenic cells that had succeeded to reach the spermatid stage. Thus, in addition to growth factors and hormones, influences that promote the biosynthesis of PUFA-containing lipids are among the factors required to optimize spermatogenesis in ex vivo tissue explants
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Isoler Alcaraz, Javier. Consejo Superior de Investigaciones Científicas; España
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: del Mazo, Jesús. Consejo Superior de Investigaciones Científicas; España
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVI Reunión de la Sociedad. Argentina de Investigación en Bioquímica y Biología Molecular y XIV Congreso de la Pan American Association of Biochemistry & Molecular Biology Societies
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Panamerican Association for Biochemistry and Molecular Biology
description Using a gas-liquid interphase culture system from neonatal mouse testes, we previously observed in ex vivo explants a relationship between the progression of spermatogenesis at both cytological and histological levels and the gene expression of some of the enzymes involved in lipid metabolism. Here, we examined by RT-qPCR the expression of two PUFA elongases (Elovl2 and Elovl4), Δ6-desaturase (Fads2), fatty acid 2- hydroxylase (Fa2h), two fatty-acid-binding proteins (Fabp3 and Fabp9) and a diacylglycerol acyltransferase (Dgat2). Testis explants from 6 days old mice cultured for 22 days evidenced progress in spermatogenesis beyond the meiotic phase in some of the tubules. Although delayed in vitro in comparison with the in vivo development, in both cases the appearance of haploid germ cells occurred concomitantly with an increase in the expression of Fabp9, Dgat2, and Fa2h. Interestingly, the genes involved in PUFA synthesis (Elovl2, Elovl4, Fads2) and transport (Fabp3) were up-regulated in the testicular explants in comparison with the in vivo situation. This suggested, as a possible cause, partial insufficiency in the culture system of the C20-C22 PUFA required as substrates by these biosynthetic enzymes. This proved to be the case, as this medium contained low proportions (less than 4%) of these fatty acids. Supplementation of ex vivo explants with a PUFA-rich total lipid extract (TL) from adult mouse testis allowed progression into meiosis at the times in culture examined. Moreover, after 22 days in culture, the TL-supplemented explants contained more tubules with spermatogenic cells that had succeeded to reach the spermatid stage. Thus, in addition to growth factors and hormones, influences that promote the biosynthesis of PUFA-containing lipids are among the factors required to optimize spermatogenesis in ex vivo tissue explants
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/217679
Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis; LVI Reunión de la Sociedad. Argentina de Investigación en Bioquímica y Biología Molecular y XIV Congreso de la Pan American Association of Biochemistry & Molecular Biology Societies; Salta; Argentina; 2019; 98-98
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/217679
identifier_str_mv Supplementation of ex vivo mouse testes explants with PUFA-RICH lipids stimulates spermatogenesis; LVI Reunión de la Sociedad. Argentina de Investigación en Bioquímica y Biología Molecular y XIV Congreso de la Pan American Association of Biochemistry & Molecular Biology Societies; Salta; Argentina; 2019; 98-98
1667-5746
CONICET Digital
CONICET
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