Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells

Autores: Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; Fischer, Roman; Charles, Philip D.; Dudek, Nadine L.; Mothe, Beatriz; Crespo, Manuel; Fischer, William M.; Korber, Bette T. M.; Nielsen, Morten; Borrow, Persephone; Purcell, Anthony W.; Brander, Christian; Dorrell, Lucy; Kessler, Benedikt M.; Hanke, Tomáš
Año de publicación: 2016
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
Fil: Ternette, Nicola. University of Oxford; Reino Unido
Fil: Yang, Hongbing. University of Oxford; Reino Unido
Fil: Partridge, Thomas. University of Oxford; Reino Unido
Fil: Llano, Anuska. Universitat Autònoma de Barcelona; España
Fil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; España
Fil: Fischer, Roman. University of Oxford; Reino Unido
Fil: Charles, Philip D.. University of Oxford; Reino Unido
Fil: Dudek, Nadine L.. Monash University; Australia
Fil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; España
Fil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; España
Fil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; Dinamarca
Fil: Borrow, Persephone. University of Oxford; Reino Unido
Fil: Purcell, Anthony W.. Monash University; Australia
Fil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; España
Fil: Dorrell, Lucy. University of Oxford; Reino Unido
Fil: Kessler, Benedikt M.. University of Oxford; Reino Unido
Fil: Hanke, Tomáš. University of Oxford; Reino Unido
Materia: CYTOTOXIC T CELLS
HUMAN IMMUNODEFICIENCY VIRUS TYPE I
HUMAN LEUKOCYTE ANTIGEN
IMMUNOPEPTIDOME
MASS SPECTROMETRY
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/53352

Acceder

id	CONICETDig_ea6821cab0ace081957cf853b39a65b5
oai_identifier_str	oai:ri.conicet.gov.ar:11336/53352
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cellsTernette, NicolaYang, HongbingPartridge, ThomasLlano, AnuskaCedeño, SamandhyFischer, RomanCharles, Philip D.Dudek, Nadine L.Mothe, BeatrizCrespo, ManuelFischer, William M.Korber, Bette T. M.Nielsen, MortenBorrow, PersephonePurcell, Anthony W.Brander, ChristianDorrell, LucyKessler, Benedikt M.Hanke, TomášCYTOTOXIC T CELLSHUMAN IMMUNODEFICIENCY VIRUS TYPE IHUMAN LEUKOCYTE ANTIGENIMMUNOPEPTIDOMEMASS SPECTROMETRYhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.Fil: Ternette, Nicola. University of Oxford; Reino UnidoFil: Yang, Hongbing. University of Oxford; Reino UnidoFil: Partridge, Thomas. University of Oxford; Reino UnidoFil: Llano, Anuska. Universitat Autònoma de Barcelona; EspañaFil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; EspañaFil: Fischer, Roman. University of Oxford; Reino UnidoFil: Charles, Philip D.. University of Oxford; Reino UnidoFil: Dudek, Nadine L.. Monash University; AustraliaFil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; EspañaFil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; EspañaFil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados UnidosFil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados UnidosFil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; DinamarcaFil: Borrow, Persephone. University of Oxford; Reino UnidoFil: Purcell, Anthony W.. Monash University; AustraliaFil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; EspañaFil: Dorrell, Lucy. University of Oxford; Reino UnidoFil: Kessler, Benedikt M.. University of Oxford; Reino UnidoFil: Hanke, Tomáš. University of Oxford; Reino UnidoWiley VCH Verlag2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53352Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-690014-2980CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/eji.201545890info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201545890info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737398/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:18:30Zoai:ri.conicet.gov.ar:11336/53352instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:18:30.958CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title	Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
spellingShingle	Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells Ternette, Nicola CYTOTOXIC T CELLS HUMAN IMMUNODEFICIENCY VIRUS TYPE I HUMAN LEUKOCYTE ANTIGEN IMMUNOPEPTIDOME MASS SPECTROMETRY
title_short	Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title_full	Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title_fullStr	Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title_full_unstemmed	Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title_sort	Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
dc.creator.none.fl_str_mv	Ternette, Nicola Yang, Hongbing Partridge, Thomas Llano, Anuska Cedeño, Samandhy Fischer, Roman Charles, Philip D. Dudek, Nadine L. Mothe, Beatriz Crespo, Manuel Fischer, William M. Korber, Bette T. M. Nielsen, Morten Borrow, Persephone Purcell, Anthony W. Brander, Christian Dorrell, Lucy Kessler, Benedikt M. Hanke, Tomáš
author	Ternette, Nicola
author_facet	Ternette, Nicola Yang, Hongbing Partridge, Thomas Llano, Anuska Cedeño, Samandhy Fischer, Roman Charles, Philip D. Dudek, Nadine L. Mothe, Beatriz Crespo, Manuel Fischer, William M. Korber, Bette T. M. Nielsen, Morten Borrow, Persephone Purcell, Anthony W. Brander, Christian Dorrell, Lucy Kessler, Benedikt M. Hanke, Tomáš
author_role	author
author2	Yang, Hongbing Partridge, Thomas Llano, Anuska Cedeño, Samandhy Fischer, Roman Charles, Philip D. Dudek, Nadine L. Mothe, Beatriz Crespo, Manuel Fischer, William M. Korber, Bette T. M. Nielsen, Morten Borrow, Persephone Purcell, Anthony W. Brander, Christian Dorrell, Lucy Kessler, Benedikt M. Hanke, Tomáš
author2_role	author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	CYTOTOXIC T CELLS HUMAN IMMUNODEFICIENCY VIRUS TYPE I HUMAN LEUKOCYTE ANTIGEN IMMUNOPEPTIDOME MASS SPECTROMETRY
topic	CYTOTOXIC T CELLS HUMAN IMMUNODEFICIENCY VIRUS TYPE I HUMAN LEUKOCYTE ANTIGEN IMMUNOPEPTIDOME MASS SPECTROMETRY
purl_subject.fl_str_mv	https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv	Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development. Fil: Ternette, Nicola. University of Oxford; Reino Unido Fil: Yang, Hongbing. University of Oxford; Reino Unido Fil: Partridge, Thomas. University of Oxford; Reino Unido Fil: Llano, Anuska. Universitat Autònoma de Barcelona; España Fil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; España Fil: Fischer, Roman. University of Oxford; Reino Unido Fil: Charles, Philip D.. University of Oxford; Reino Unido Fil: Dudek, Nadine L.. Monash University; Australia Fil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; España Fil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; España Fil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos Fil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos Fil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; Dinamarca Fil: Borrow, Persephone. University of Oxford; Reino Unido Fil: Purcell, Anthony W.. Monash University; Australia Fil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; España Fil: Dorrell, Lucy. University of Oxford; Reino Unido Fil: Kessler, Benedikt M.. University of Oxford; Reino Unido Fil: Hanke, Tomáš. University of Oxford; Reino Unido
description	Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
publishDate	2016
dc.date.none.fl_str_mv	2016-01
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/53352 Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-69 0014-2980 CONICET Digital CONICET
url	http://hdl.handle.net/11336/53352
identifier_str_mv	Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-69 0014-2980 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1002/eji.201545890 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201545890 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737398/
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Wiley VCH Verlag
publisher.none.fl_str_mv	Wiley VCH Verlag
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1846781648799006720
score	12.982451

Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells

Publicaciones similares