Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells

Autores
Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; Fischer, Roman; Charles, Philip D.; Dudek, Nadine L.; Mothe, Beatriz; Crespo, Manuel; Fischer, William M.; Korber, Bette T. M.; Nielsen, Morten; Borrow, Persephone; Purcell, Anthony W.; Brander, Christian; Dorrell, Lucy; Kessler, Benedikt M.; Hanke, Tomáš
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
Fil: Ternette, Nicola. University of Oxford; Reino Unido
Fil: Yang, Hongbing. University of Oxford; Reino Unido
Fil: Partridge, Thomas. University of Oxford; Reino Unido
Fil: Llano, Anuska. Universitat Autònoma de Barcelona; España
Fil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; España
Fil: Fischer, Roman. University of Oxford; Reino Unido
Fil: Charles, Philip D.. University of Oxford; Reino Unido
Fil: Dudek, Nadine L.. Monash University; Australia
Fil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; España
Fil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; España
Fil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; Dinamarca
Fil: Borrow, Persephone. University of Oxford; Reino Unido
Fil: Purcell, Anthony W.. Monash University; Australia
Fil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; España
Fil: Dorrell, Lucy. University of Oxford; Reino Unido
Fil: Kessler, Benedikt M.. University of Oxford; Reino Unido
Fil: Hanke, Tomáš. University of Oxford; Reino Unido
Materia
CYTOTOXIC T CELLS
HUMAN IMMUNODEFICIENCY VIRUS TYPE I
HUMAN LEUKOCYTE ANTIGEN
IMMUNOPEPTIDOME
MASS SPECTROMETRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53352

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oai_identifier_str oai:ri.conicet.gov.ar:11336/53352
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cellsTernette, NicolaYang, HongbingPartridge, ThomasLlano, AnuskaCedeño, SamandhyFischer, RomanCharles, Philip D.Dudek, Nadine L.Mothe, BeatrizCrespo, ManuelFischer, William M.Korber, Bette T. M.Nielsen, MortenBorrow, PersephonePurcell, Anthony W.Brander, ChristianDorrell, LucyKessler, Benedikt M.Hanke, TomášCYTOTOXIC T CELLSHUMAN IMMUNODEFICIENCY VIRUS TYPE IHUMAN LEUKOCYTE ANTIGENIMMUNOPEPTIDOMEMASS SPECTROMETRYhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.Fil: Ternette, Nicola. University of Oxford; Reino UnidoFil: Yang, Hongbing. University of Oxford; Reino UnidoFil: Partridge, Thomas. University of Oxford; Reino UnidoFil: Llano, Anuska. Universitat Autònoma de Barcelona; EspañaFil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; EspañaFil: Fischer, Roman. University of Oxford; Reino UnidoFil: Charles, Philip D.. University of Oxford; Reino UnidoFil: Dudek, Nadine L.. Monash University; AustraliaFil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; EspañaFil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; EspañaFil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados UnidosFil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados UnidosFil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; DinamarcaFil: Borrow, Persephone. University of Oxford; Reino UnidoFil: Purcell, Anthony W.. Monash University; AustraliaFil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; EspañaFil: Dorrell, Lucy. University of Oxford; Reino UnidoFil: Kessler, Benedikt M.. University of Oxford; Reino UnidoFil: Hanke, Tomáš. University of Oxford; Reino UnidoWiley VCH Verlag2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53352Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-690014-2980CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/eji.201545890info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201545890info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737398/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:03Zoai:ri.conicet.gov.ar:11336/53352instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:03.922CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
spellingShingle Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
Ternette, Nicola
CYTOTOXIC T CELLS
HUMAN IMMUNODEFICIENCY VIRUS TYPE I
HUMAN LEUKOCYTE ANTIGEN
IMMUNOPEPTIDOME
MASS SPECTROMETRY
title_short Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title_full Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title_fullStr Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title_full_unstemmed Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
title_sort Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
dc.creator.none.fl_str_mv Ternette, Nicola
Yang, Hongbing
Partridge, Thomas
Llano, Anuska
Cedeño, Samandhy
Fischer, Roman
Charles, Philip D.
Dudek, Nadine L.
Mothe, Beatriz
Crespo, Manuel
Fischer, William M.
Korber, Bette T. M.
Nielsen, Morten
Borrow, Persephone
Purcell, Anthony W.
Brander, Christian
Dorrell, Lucy
Kessler, Benedikt M.
Hanke, Tomáš
author Ternette, Nicola
author_facet Ternette, Nicola
Yang, Hongbing
Partridge, Thomas
Llano, Anuska
Cedeño, Samandhy
Fischer, Roman
Charles, Philip D.
Dudek, Nadine L.
Mothe, Beatriz
Crespo, Manuel
Fischer, William M.
Korber, Bette T. M.
Nielsen, Morten
Borrow, Persephone
Purcell, Anthony W.
Brander, Christian
Dorrell, Lucy
Kessler, Benedikt M.
Hanke, Tomáš
author_role author
author2 Yang, Hongbing
Partridge, Thomas
Llano, Anuska
Cedeño, Samandhy
Fischer, Roman
Charles, Philip D.
Dudek, Nadine L.
Mothe, Beatriz
Crespo, Manuel
Fischer, William M.
Korber, Bette T. M.
Nielsen, Morten
Borrow, Persephone
Purcell, Anthony W.
Brander, Christian
Dorrell, Lucy
Kessler, Benedikt M.
Hanke, Tomáš
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CYTOTOXIC T CELLS
HUMAN IMMUNODEFICIENCY VIRUS TYPE I
HUMAN LEUKOCYTE ANTIGEN
IMMUNOPEPTIDOME
MASS SPECTROMETRY
topic CYTOTOXIC T CELLS
HUMAN IMMUNODEFICIENCY VIRUS TYPE I
HUMAN LEUKOCYTE ANTIGEN
IMMUNOPEPTIDOME
MASS SPECTROMETRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
Fil: Ternette, Nicola. University of Oxford; Reino Unido
Fil: Yang, Hongbing. University of Oxford; Reino Unido
Fil: Partridge, Thomas. University of Oxford; Reino Unido
Fil: Llano, Anuska. Universitat Autònoma de Barcelona; España
Fil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; España
Fil: Fischer, Roman. University of Oxford; Reino Unido
Fil: Charles, Philip D.. University of Oxford; Reino Unido
Fil: Dudek, Nadine L.. Monash University; Australia
Fil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; España
Fil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; España
Fil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; Dinamarca
Fil: Borrow, Persephone. University of Oxford; Reino Unido
Fil: Purcell, Anthony W.. Monash University; Australia
Fil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; España
Fil: Dorrell, Lucy. University of Oxford; Reino Unido
Fil: Kessler, Benedikt M.. University of Oxford; Reino Unido
Fil: Hanke, Tomáš. University of Oxford; Reino Unido
description Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53352
Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-69
0014-2980
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53352
identifier_str_mv Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-69
0014-2980
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/eji.201545890
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201545890
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737398/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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