Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
- Autores
- Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; Fischer, Roman; Charles, Philip D.; Dudek, Nadine L.; Mothe, Beatriz; Crespo, Manuel; Fischer, William M.; Korber, Bette T. M.; Nielsen, Morten; Borrow, Persephone; Purcell, Anthony W.; Brander, Christian; Dorrell, Lucy; Kessler, Benedikt M.; Hanke, Tomáš
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
Fil: Ternette, Nicola. University of Oxford; Reino Unido
Fil: Yang, Hongbing. University of Oxford; Reino Unido
Fil: Partridge, Thomas. University of Oxford; Reino Unido
Fil: Llano, Anuska. Universitat Autònoma de Barcelona; España
Fil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; España
Fil: Fischer, Roman. University of Oxford; Reino Unido
Fil: Charles, Philip D.. University of Oxford; Reino Unido
Fil: Dudek, Nadine L.. Monash University; Australia
Fil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; España
Fil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; España
Fil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; Dinamarca
Fil: Borrow, Persephone. University of Oxford; Reino Unido
Fil: Purcell, Anthony W.. Monash University; Australia
Fil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; España
Fil: Dorrell, Lucy. University of Oxford; Reino Unido
Fil: Kessler, Benedikt M.. University of Oxford; Reino Unido
Fil: Hanke, Tomáš. University of Oxford; Reino Unido - Materia
-
CYTOTOXIC T CELLS
HUMAN IMMUNODEFICIENCY VIRUS TYPE I
HUMAN LEUKOCYTE ANTIGEN
IMMUNOPEPTIDOME
MASS SPECTROMETRY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/53352
Ver los metadatos del registro completo
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Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cellsTernette, NicolaYang, HongbingPartridge, ThomasLlano, AnuskaCedeño, SamandhyFischer, RomanCharles, Philip D.Dudek, Nadine L.Mothe, BeatrizCrespo, ManuelFischer, William M.Korber, Bette T. M.Nielsen, MortenBorrow, PersephonePurcell, Anthony W.Brander, ChristianDorrell, LucyKessler, Benedikt M.Hanke, TomášCYTOTOXIC T CELLSHUMAN IMMUNODEFICIENCY VIRUS TYPE IHUMAN LEUKOCYTE ANTIGENIMMUNOPEPTIDOMEMASS SPECTROMETRYhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.Fil: Ternette, Nicola. University of Oxford; Reino UnidoFil: Yang, Hongbing. University of Oxford; Reino UnidoFil: Partridge, Thomas. University of Oxford; Reino UnidoFil: Llano, Anuska. Universitat Autònoma de Barcelona; EspañaFil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; EspañaFil: Fischer, Roman. University of Oxford; Reino UnidoFil: Charles, Philip D.. University of Oxford; Reino UnidoFil: Dudek, Nadine L.. Monash University; AustraliaFil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; EspañaFil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; EspañaFil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados UnidosFil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados UnidosFil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; DinamarcaFil: Borrow, Persephone. University of Oxford; Reino UnidoFil: Purcell, Anthony W.. Monash University; AustraliaFil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; EspañaFil: Dorrell, Lucy. University of Oxford; Reino UnidoFil: Kessler, Benedikt M.. University of Oxford; Reino UnidoFil: Hanke, Tomáš. University of Oxford; Reino UnidoWiley VCH Verlag2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53352Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-690014-2980CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/eji.201545890info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201545890info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737398/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:03Zoai:ri.conicet.gov.ar:11336/53352instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:03.922CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
title |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
spellingShingle |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells Ternette, Nicola CYTOTOXIC T CELLS HUMAN IMMUNODEFICIENCY VIRUS TYPE I HUMAN LEUKOCYTE ANTIGEN IMMUNOPEPTIDOME MASS SPECTROMETRY |
title_short |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
title_full |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
title_fullStr |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
title_full_unstemmed |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
title_sort |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
dc.creator.none.fl_str_mv |
Ternette, Nicola Yang, Hongbing Partridge, Thomas Llano, Anuska Cedeño, Samandhy Fischer, Roman Charles, Philip D. Dudek, Nadine L. Mothe, Beatriz Crespo, Manuel Fischer, William M. Korber, Bette T. M. Nielsen, Morten Borrow, Persephone Purcell, Anthony W. Brander, Christian Dorrell, Lucy Kessler, Benedikt M. Hanke, Tomáš |
author |
Ternette, Nicola |
author_facet |
Ternette, Nicola Yang, Hongbing Partridge, Thomas Llano, Anuska Cedeño, Samandhy Fischer, Roman Charles, Philip D. Dudek, Nadine L. Mothe, Beatriz Crespo, Manuel Fischer, William M. Korber, Bette T. M. Nielsen, Morten Borrow, Persephone Purcell, Anthony W. Brander, Christian Dorrell, Lucy Kessler, Benedikt M. Hanke, Tomáš |
author_role |
author |
author2 |
Yang, Hongbing Partridge, Thomas Llano, Anuska Cedeño, Samandhy Fischer, Roman Charles, Philip D. Dudek, Nadine L. Mothe, Beatriz Crespo, Manuel Fischer, William M. Korber, Bette T. M. Nielsen, Morten Borrow, Persephone Purcell, Anthony W. Brander, Christian Dorrell, Lucy Kessler, Benedikt M. Hanke, Tomáš |
author2_role |
author author author author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
CYTOTOXIC T CELLS HUMAN IMMUNODEFICIENCY VIRUS TYPE I HUMAN LEUKOCYTE ANTIGEN IMMUNOPEPTIDOME MASS SPECTROMETRY |
topic |
CYTOTOXIC T CELLS HUMAN IMMUNODEFICIENCY VIRUS TYPE I HUMAN LEUKOCYTE ANTIGEN IMMUNOPEPTIDOME MASS SPECTROMETRY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development. Fil: Ternette, Nicola. University of Oxford; Reino Unido Fil: Yang, Hongbing. University of Oxford; Reino Unido Fil: Partridge, Thomas. University of Oxford; Reino Unido Fil: Llano, Anuska. Universitat Autònoma de Barcelona; España Fil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; España Fil: Fischer, Roman. University of Oxford; Reino Unido Fil: Charles, Philip D.. University of Oxford; Reino Unido Fil: Dudek, Nadine L.. Monash University; Australia Fil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; España Fil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; España Fil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos Fil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos Fil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; Dinamarca Fil: Borrow, Persephone. University of Oxford; Reino Unido Fil: Purcell, Anthony W.. Monash University; Australia Fil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; España Fil: Dorrell, Lucy. University of Oxford; Reino Unido Fil: Kessler, Benedikt M.. University of Oxford; Reino Unido Fil: Hanke, Tomáš. University of Oxford; Reino Unido |
description |
Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/53352 Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-69 0014-2980 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/53352 |
identifier_str_mv |
Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-69 0014-2980 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1002/eji.201545890 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201545890 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737398/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley VCH Verlag |
publisher.none.fl_str_mv |
Wiley VCH Verlag |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613520521428992 |
score |
13.070432 |