Saliva direct PCR protocol for HLA‐DQB1*02 genotyping

Autores
Carrillo, Angeles; Manzur, Maria Jimena; Juri Ayub, Maximiliano
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies.
Fil: Carrillo, Angeles. Universidad Nacional de San Luis; Argentina
Fil: Manzur, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Materia
autoimmune
disease
human leucocyte antigen
immunogenetics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/257195
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/257195
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Saliva direct PCR protocol for HLA‐DQB1*02 genotypingCarrillo, AngelesManzur, Maria JimenaJuri Ayub, Maximilianoautoimmunediseasehuman leucocyte antigenimmunogeneticshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies.Fil: Carrillo, Angeles. Universidad Nacional de San Luis; ArgentinaFil: Manzur, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaWiley Blackwell Publishing, Inc2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/257195Carrillo, Angeles; Manzur, Maria Jimena; Juri Ayub, Maximiliano; Saliva direct PCR protocol for HLA‐DQB1*02 genotyping; Wiley Blackwell Publishing, Inc; International Journal of Immunogenetics; 51; 2; 1-2024; 89-921744-3121CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/iji.12656info:eu-repo/semantics/altIdentifier/doi/10.1111/iji.12656info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:02:39Zoai:ri.conicet.gov.ar:11336/257195instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:02:39.814CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
title Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
spellingShingle Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
Carrillo, Angeles
autoimmune
disease
human leucocyte antigen
immunogenetics
title_short Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
title_full Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
title_fullStr Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
title_full_unstemmed Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
title_sort Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
dc.creator.none.fl_str_mv Carrillo, Angeles
Manzur, Maria Jimena
Juri Ayub, Maximiliano
author Carrillo, Angeles
author_facet Carrillo, Angeles
Manzur, Maria Jimena
Juri Ayub, Maximiliano
author_role author
author2 Manzur, Maria Jimena
Juri Ayub, Maximiliano
author2_role author
author
dc.subject.none.fl_str_mv autoimmune
disease
human leucocyte antigen
immunogenetics
topic autoimmune
disease
human leucocyte antigen
immunogenetics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies.
Fil: Carrillo, Angeles. Universidad Nacional de San Luis; Argentina
Fil: Manzur, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
description Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies.
publishDate 2024
dc.date.none.fl_str_mv 2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/257195
Carrillo, Angeles; Manzur, Maria Jimena; Juri Ayub, Maximiliano; Saliva direct PCR protocol for HLA‐DQB1*02 genotyping; Wiley Blackwell Publishing, Inc; International Journal of Immunogenetics; 51; 2; 1-2024; 89-92
1744-3121
CONICET Digital
CONICET
url http://hdl.handle.net/11336/257195
identifier_str_mv Carrillo, Angeles; Manzur, Maria Jimena; Juri Ayub, Maximiliano; Saliva direct PCR protocol for HLA‐DQB1*02 genotyping; Wiley Blackwell Publishing, Inc; International Journal of Immunogenetics; 51; 2; 1-2024; 89-92
1744-3121
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/iji.12656
info:eu-repo/semantics/altIdentifier/doi/10.1111/iji.12656
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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