Saliva direct PCR protocol for HLA‐DQB1*02 genotyping
- Autores
- Carrillo, Angeles; Manzur, Maria Jimena; Juri Ayub, Maximiliano
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies.
Fil: Carrillo, Angeles. Universidad Nacional de San Luis; Argentina
Fil: Manzur, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina - Materia
-
autoimmune
disease
human leucocyte antigen
immunogenetics - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/257195
Ver los metadatos del registro completo
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Saliva direct PCR protocol for HLA‐DQB1*02 genotypingCarrillo, AngelesManzur, Maria JimenaJuri Ayub, Maximilianoautoimmunediseasehuman leucocyte antigenimmunogeneticshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies.Fil: Carrillo, Angeles. Universidad Nacional de San Luis; ArgentinaFil: Manzur, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaWiley Blackwell Publishing, Inc2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/257195Carrillo, Angeles; Manzur, Maria Jimena; Juri Ayub, Maximiliano; Saliva direct PCR protocol for HLA‐DQB1*02 genotyping; Wiley Blackwell Publishing, Inc; International Journal of Immunogenetics; 51; 2; 1-2024; 89-921744-3121CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/iji.12656info:eu-repo/semantics/altIdentifier/doi/10.1111/iji.12656info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:38Zoai:ri.conicet.gov.ar:11336/257195instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:38.517CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping |
title |
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping |
spellingShingle |
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping Carrillo, Angeles autoimmune disease human leucocyte antigen immunogenetics |
title_short |
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping |
title_full |
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping |
title_fullStr |
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping |
title_full_unstemmed |
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping |
title_sort |
Saliva direct PCR protocol for HLA‐DQB1*02 genotyping |
dc.creator.none.fl_str_mv |
Carrillo, Angeles Manzur, Maria Jimena Juri Ayub, Maximiliano |
author |
Carrillo, Angeles |
author_facet |
Carrillo, Angeles Manzur, Maria Jimena Juri Ayub, Maximiliano |
author_role |
author |
author2 |
Manzur, Maria Jimena Juri Ayub, Maximiliano |
author2_role |
author author |
dc.subject.none.fl_str_mv |
autoimmune disease human leucocyte antigen immunogenetics |
topic |
autoimmune disease human leucocyte antigen immunogenetics |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies. Fil: Carrillo, Angeles. Universidad Nacional de San Luis; Argentina Fil: Manzur, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina |
description |
Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/257195 Carrillo, Angeles; Manzur, Maria Jimena; Juri Ayub, Maximiliano; Saliva direct PCR protocol for HLA‐DQB1*02 genotyping; Wiley Blackwell Publishing, Inc; International Journal of Immunogenetics; 51; 2; 1-2024; 89-92 1744-3121 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/257195 |
identifier_str_mv |
Carrillo, Angeles; Manzur, Maria Jimena; Juri Ayub, Maximiliano; Saliva direct PCR protocol for HLA‐DQB1*02 genotyping; Wiley Blackwell Publishing, Inc; International Journal of Immunogenetics; 51; 2; 1-2024; 89-92 1744-3121 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/iji.12656 info:eu-repo/semantics/altIdentifier/doi/10.1111/iji.12656 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613073256579072 |
score |
13.070432 |