Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in...

Autores
Vitale, Daiana Luján; Caon, Ilaria; Parnigoni, Arianna; Sevic, Ina; Spinelli, Fiorella Mercedes; Icardi, Antonella; Passi, Alberto; Vigetti, Davide; Alaniz, Laura Daniela
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
UDP-glucose-dehydrogenase (UGDH) synthesizes UDP-glucuronic acid. It is involved in epirubicin detoxification and hyaluronan synthesis. This work aimed to evaluate the effect of UGDH knockdown on epirubicin response and hyaluronan metabolism in MDA-MB-231 breast cancer cells. Additionally, the aim was to determine UGDH as a possible prognosis marker in breast cancer. We studied UGDH expression in tumors and adjacent tissue from breast cancer patients. The prognostic value of UGDH was studied using a public Kaplan–Meier plotter. MDA-MB-231 cells were knocked-down for UGDH and treated with epirubicin. Epirubicin-accumulation and apoptosis were analyzed by flow cytometry. Hyaluronan-coated matrix and metabolism were determined. Autophagic-LC3-II was studied by Western blot and confocal microscopy. Epirubicin accumulation increased and apoptosis decreased during UGDH knockdown. Hyaluronan-coated matrix increased and a positive modulation of autophagy was detected. Higher levels of UGDH were correlated with worse prognosis in triple-negative breast cancer patients that received chemotherapy. High expression of UGDH was found in tumoral tissue from HER2--patients. However, UGDH knockdown contributes to epirubicin resistance, which might be associated with increases in the expression, deposition and catabolism of hyaluronan. The results obtained allowed us to propose UGDH as a new prognostic marker in breast cancer, positively associated with development of epirubicin resistance and modulation of extracellular matrix.
Fil: Vitale, Daiana Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Caon, Ilaria. Università degli Studio dell’Insubria; Italia
Fil: Parnigoni, Arianna. Università degli Studio dell’Insubria; Italia
Fil: Sevic, Ina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Spinelli, Fiorella Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Icardi, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Passi, Alberto. Università degli Studio dell’Insubria; Italia
Fil: Vigetti, Davide. Università degli Studio dell’Insubria; Italia
Fil: Alaniz, Laura Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Materia
BREAST CANCER
DRUG RESISTANCE
EPIRUBICIN
EXTRACELLULAR MATRIX
HYALURONAN
UDP-GLUCOSE DEHYDROGENASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/166606
Acceder
id CONICETDig_ea5233f311ec830e7221af6f5773c93c
oai_identifier_str oai:ri.conicet.gov.ar:11336/166606
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cellsVitale, Daiana LujánCaon, IlariaParnigoni, AriannaSevic, InaSpinelli, Fiorella MercedesIcardi, AntonellaPassi, AlbertoVigetti, DavideAlaniz, Laura DanielaBREAST CANCERDRUG RESISTANCEEPIRUBICINEXTRACELLULAR MATRIXHYALURONANUDP-GLUCOSE DEHYDROGENASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1UDP-glucose-dehydrogenase (UGDH) synthesizes UDP-glucuronic acid. It is involved in epirubicin detoxification and hyaluronan synthesis. This work aimed to evaluate the effect of UGDH knockdown on epirubicin response and hyaluronan metabolism in MDA-MB-231 breast cancer cells. Additionally, the aim was to determine UGDH as a possible prognosis marker in breast cancer. We studied UGDH expression in tumors and adjacent tissue from breast cancer patients. The prognostic value of UGDH was studied using a public Kaplan–Meier plotter. MDA-MB-231 cells were knocked-down for UGDH and treated with epirubicin. Epirubicin-accumulation and apoptosis were analyzed by flow cytometry. Hyaluronan-coated matrix and metabolism were determined. Autophagic-LC3-II was studied by Western blot and confocal microscopy. Epirubicin accumulation increased and apoptosis decreased during UGDH knockdown. Hyaluronan-coated matrix increased and a positive modulation of autophagy was detected. Higher levels of UGDH were correlated with worse prognosis in triple-negative breast cancer patients that received chemotherapy. High expression of UGDH was found in tumoral tissue from HER2--patients. However, UGDH knockdown contributes to epirubicin resistance, which might be associated with increases in the expression, deposition and catabolism of hyaluronan. The results obtained allowed us to propose UGDH as a new prognostic marker in breast cancer, positively associated with development of epirubicin resistance and modulation of extracellular matrix.Fil: Vitale, Daiana Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Caon, Ilaria. Università degli Studio dell’Insubria; ItaliaFil: Parnigoni, Arianna. Università degli Studio dell’Insubria; ItaliaFil: Sevic, Ina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Spinelli, Fiorella Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Icardi, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Passi, Alberto. Università degli Studio dell’Insubria; ItaliaFil: Vigetti, Davide. Università degli Studio dell’Insubria; ItaliaFil: Alaniz, Laura Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaMultidisciplinary Digital Publishing Institute2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/166606Vitale, Daiana Luján; Caon, Ilaria; Parnigoni, Arianna; Sevic, Ina; Spinelli, Fiorella Mercedes; et al.; Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells; Multidisciplinary Digital Publishing Institute; Biomolecules; 11; 2; 2-2021; 1-312218-273X2218-273XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-273X/11/2/246info:eu-repo/semantics/altIdentifier/doi/10.3390/biom11020246info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:03Zoai:ri.conicet.gov.ar:11336/166606instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:03.82CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells
title Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells
spellingShingle Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells
Vitale, Daiana Luján
BREAST CANCER
DRUG RESISTANCE
EPIRUBICIN
EXTRACELLULAR MATRIX
HYALURONAN
UDP-GLUCOSE DEHYDROGENASE
title_short Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells
title_full Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells
title_fullStr Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells
title_full_unstemmed Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells
title_sort Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells
dc.creator.none.fl_str_mv Vitale, Daiana Luján
Caon, Ilaria
Parnigoni, Arianna
Sevic, Ina
Spinelli, Fiorella Mercedes
Icardi, Antonella
Passi, Alberto
Vigetti, Davide
Alaniz, Laura Daniela
author Vitale, Daiana Luján
author_facet Vitale, Daiana Luján
Caon, Ilaria
Parnigoni, Arianna
Sevic, Ina
Spinelli, Fiorella Mercedes
Icardi, Antonella
Passi, Alberto
Vigetti, Davide
Alaniz, Laura Daniela
author_role author
author2 Caon, Ilaria
Parnigoni, Arianna
Sevic, Ina
Spinelli, Fiorella Mercedes
Icardi, Antonella
Passi, Alberto
Vigetti, Davide
Alaniz, Laura Daniela
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BREAST CANCER
DRUG RESISTANCE
EPIRUBICIN
EXTRACELLULAR MATRIX
HYALURONAN
UDP-GLUCOSE DEHYDROGENASE
topic BREAST CANCER
DRUG RESISTANCE
EPIRUBICIN
EXTRACELLULAR MATRIX
HYALURONAN
UDP-GLUCOSE DEHYDROGENASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv UDP-glucose-dehydrogenase (UGDH) synthesizes UDP-glucuronic acid. It is involved in epirubicin detoxification and hyaluronan synthesis. This work aimed to evaluate the effect of UGDH knockdown on epirubicin response and hyaluronan metabolism in MDA-MB-231 breast cancer cells. Additionally, the aim was to determine UGDH as a possible prognosis marker in breast cancer. We studied UGDH expression in tumors and adjacent tissue from breast cancer patients. The prognostic value of UGDH was studied using a public Kaplan–Meier plotter. MDA-MB-231 cells were knocked-down for UGDH and treated with epirubicin. Epirubicin-accumulation and apoptosis were analyzed by flow cytometry. Hyaluronan-coated matrix and metabolism were determined. Autophagic-LC3-II was studied by Western blot and confocal microscopy. Epirubicin accumulation increased and apoptosis decreased during UGDH knockdown. Hyaluronan-coated matrix increased and a positive modulation of autophagy was detected. Higher levels of UGDH were correlated with worse prognosis in triple-negative breast cancer patients that received chemotherapy. High expression of UGDH was found in tumoral tissue from HER2--patients. However, UGDH knockdown contributes to epirubicin resistance, which might be associated with increases in the expression, deposition and catabolism of hyaluronan. The results obtained allowed us to propose UGDH as a new prognostic marker in breast cancer, positively associated with development of epirubicin resistance and modulation of extracellular matrix.
Fil: Vitale, Daiana Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Caon, Ilaria. Università degli Studio dell’Insubria; Italia
Fil: Parnigoni, Arianna. Università degli Studio dell’Insubria; Italia
Fil: Sevic, Ina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Spinelli, Fiorella Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Icardi, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Passi, Alberto. Università degli Studio dell’Insubria; Italia
Fil: Vigetti, Davide. Università degli Studio dell’Insubria; Italia
Fil: Alaniz, Laura Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
description UDP-glucose-dehydrogenase (UGDH) synthesizes UDP-glucuronic acid. It is involved in epirubicin detoxification and hyaluronan synthesis. This work aimed to evaluate the effect of UGDH knockdown on epirubicin response and hyaluronan metabolism in MDA-MB-231 breast cancer cells. Additionally, the aim was to determine UGDH as a possible prognosis marker in breast cancer. We studied UGDH expression in tumors and adjacent tissue from breast cancer patients. The prognostic value of UGDH was studied using a public Kaplan–Meier plotter. MDA-MB-231 cells were knocked-down for UGDH and treated with epirubicin. Epirubicin-accumulation and apoptosis were analyzed by flow cytometry. Hyaluronan-coated matrix and metabolism were determined. Autophagic-LC3-II was studied by Western blot and confocal microscopy. Epirubicin accumulation increased and apoptosis decreased during UGDH knockdown. Hyaluronan-coated matrix increased and a positive modulation of autophagy was detected. Higher levels of UGDH were correlated with worse prognosis in triple-negative breast cancer patients that received chemotherapy. High expression of UGDH was found in tumoral tissue from HER2--patients. However, UGDH knockdown contributes to epirubicin resistance, which might be associated with increases in the expression, deposition and catabolism of hyaluronan. The results obtained allowed us to propose UGDH as a new prognostic marker in breast cancer, positively associated with development of epirubicin resistance and modulation of extracellular matrix.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/166606
Vitale, Daiana Luján; Caon, Ilaria; Parnigoni, Arianna; Sevic, Ina; Spinelli, Fiorella Mercedes; et al.; Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells; Multidisciplinary Digital Publishing Institute; Biomolecules; 11; 2; 2-2021; 1-31
2218-273X
2218-273X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/166606
identifier_str_mv Vitale, Daiana Luján; Caon, Ilaria; Parnigoni, Arianna; Sevic, Ina; Spinelli, Fiorella Mercedes; et al.; Initial identification of UDP-glucose dehydrogenase as a prognostic marker in breast cancer patients, which facilitates epirubicin resistance and regulates hyaluronan synthesis in MDA-MB-231 cells; Multidisciplinary Digital Publishing Institute; Biomolecules; 11; 2; 2-2021; 1-31
2218-273X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-273X/11/2/246
info:eu-repo/semantics/altIdentifier/doi/10.3390/biom11020246
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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