Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy

Autores
Calles, Javier Adrián; Tartara, Luis Ignacio; Lopez Garcia, Alberto Raul; Diebold Luque, Yolanda; Palma, Santiago Daniel; Valles, Enrique Marcelo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
New hyaluronic acid (HA)–itaconic acid (IT) films have been previously synthesized and used as potential topical drug delivery systems (DDS) for ocular administration. In this study we explored homogeneous and heterogeneous crosslinking reactions of HA using glutaraldehyde (GTA) and polyethylene glycol diglycidyl ether (PEGDE) in the presence of IT, a naturally occurring compound that is non-toxic and readily biodegradable. We have studied the morphology, mechanical properties and in vitro biocompatibility between these new materials and ocular surface cells (human corneal epithelial cell line) and evaluated the biopharmaceutical performance of the designed formulations. Although all the synthesized materials exhibited good mechanical properties, the PEGDE modified films exhibited the best biocompatibility, with in vivo assays showing good adhesive performance and minimal irritation. PEGDE films were also tested for their effects in the treatment of intraocular pressure (IOP) in rabbits using timolol maleate (TM) as the model drug. These results may be useful for further design of novel bioadhesive matrix containing drugs by topical application in ophthalmology.
Fil: Calles, Javier Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Tartara, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Lopez Garcia, Alberto Raul. Universidad de Valladolid; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diebold Luque, Yolanda. Universidad de Valladolid; España
Fil: Palma, Santiago Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Valles, Enrique Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Materia
Hyaluronan
Glaucoma
Bioadhesive
Polymer
Drug Delivery
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24159
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24159
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapyCalles, Javier AdriánTartara, Luis IgnacioLopez Garcia, Alberto RaulDiebold Luque, YolandaPalma, Santiago DanielValles, Enrique MarceloHyaluronanGlaucomaBioadhesivePolymerDrug Deliveryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1New hyaluronic acid (HA)–itaconic acid (IT) films have been previously synthesized and used as potential topical drug delivery systems (DDS) for ocular administration. In this study we explored homogeneous and heterogeneous crosslinking reactions of HA using glutaraldehyde (GTA) and polyethylene glycol diglycidyl ether (PEGDE) in the presence of IT, a naturally occurring compound that is non-toxic and readily biodegradable. We have studied the morphology, mechanical properties and in vitro biocompatibility between these new materials and ocular surface cells (human corneal epithelial cell line) and evaluated the biopharmaceutical performance of the designed formulations. Although all the synthesized materials exhibited good mechanical properties, the PEGDE modified films exhibited the best biocompatibility, with in vivo assays showing good adhesive performance and minimal irritation. PEGDE films were also tested for their effects in the treatment of intraocular pressure (IOP) in rabbits using timolol maleate (TM) as the model drug. These results may be useful for further design of novel bioadhesive matrix containing drugs by topical application in ophthalmology.Fil: Calles, Javier Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Tartara, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Lopez Garcia, Alberto Raul. Universidad de Valladolid; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diebold Luque, Yolanda. Universidad de Valladolid; EspañaFil: Palma, Santiago Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Valles, Enrique Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaElsevier Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24159Calles, Javier Adrián; Tartara, Luis Ignacio; Lopez Garcia, Alberto Raul; Diebold Luque, Yolanda; Palma, Santiago Daniel; et al.; Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy; Elsevier Science; International Journal Of Pharmaceutics; 455; 1-2; 8-2013; 48-560378-5173CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpharm.2013.07.063info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378517313006893info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:14Zoai:ri.conicet.gov.ar:11336/24159instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:14.559CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy
title Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy
spellingShingle Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy
Calles, Javier Adrián
Hyaluronan
Glaucoma
Bioadhesive
Polymer
Drug Delivery
title_short Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy
title_full Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy
title_fullStr Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy
title_full_unstemmed Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy
title_sort Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy
dc.creator.none.fl_str_mv Calles, Javier Adrián
Tartara, Luis Ignacio
Lopez Garcia, Alberto Raul
Diebold Luque, Yolanda
Palma, Santiago Daniel
Valles, Enrique Marcelo
author Calles, Javier Adrián
author_facet Calles, Javier Adrián
Tartara, Luis Ignacio
Lopez Garcia, Alberto Raul
Diebold Luque, Yolanda
Palma, Santiago Daniel
Valles, Enrique Marcelo
author_role author
author2 Tartara, Luis Ignacio
Lopez Garcia, Alberto Raul
Diebold Luque, Yolanda
Palma, Santiago Daniel
Valles, Enrique Marcelo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hyaluronan
Glaucoma
Bioadhesive
Polymer
Drug Delivery
topic Hyaluronan
Glaucoma
Bioadhesive
Polymer
Drug Delivery
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv New hyaluronic acid (HA)–itaconic acid (IT) films have been previously synthesized and used as potential topical drug delivery systems (DDS) for ocular administration. In this study we explored homogeneous and heterogeneous crosslinking reactions of HA using glutaraldehyde (GTA) and polyethylene glycol diglycidyl ether (PEGDE) in the presence of IT, a naturally occurring compound that is non-toxic and readily biodegradable. We have studied the morphology, mechanical properties and in vitro biocompatibility between these new materials and ocular surface cells (human corneal epithelial cell line) and evaluated the biopharmaceutical performance of the designed formulations. Although all the synthesized materials exhibited good mechanical properties, the PEGDE modified films exhibited the best biocompatibility, with in vivo assays showing good adhesive performance and minimal irritation. PEGDE films were also tested for their effects in the treatment of intraocular pressure (IOP) in rabbits using timolol maleate (TM) as the model drug. These results may be useful for further design of novel bioadhesive matrix containing drugs by topical application in ophthalmology.
Fil: Calles, Javier Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Tartara, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Lopez Garcia, Alberto Raul. Universidad de Valladolid; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diebold Luque, Yolanda. Universidad de Valladolid; España
Fil: Palma, Santiago Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Valles, Enrique Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
description New hyaluronic acid (HA)–itaconic acid (IT) films have been previously synthesized and used as potential topical drug delivery systems (DDS) for ocular administration. In this study we explored homogeneous and heterogeneous crosslinking reactions of HA using glutaraldehyde (GTA) and polyethylene glycol diglycidyl ether (PEGDE) in the presence of IT, a naturally occurring compound that is non-toxic and readily biodegradable. We have studied the morphology, mechanical properties and in vitro biocompatibility between these new materials and ocular surface cells (human corneal epithelial cell line) and evaluated the biopharmaceutical performance of the designed formulations. Although all the synthesized materials exhibited good mechanical properties, the PEGDE modified films exhibited the best biocompatibility, with in vivo assays showing good adhesive performance and minimal irritation. PEGDE films were also tested for their effects in the treatment of intraocular pressure (IOP) in rabbits using timolol maleate (TM) as the model drug. These results may be useful for further design of novel bioadhesive matrix containing drugs by topical application in ophthalmology.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24159
Calles, Javier Adrián; Tartara, Luis Ignacio; Lopez Garcia, Alberto Raul; Diebold Luque, Yolanda; Palma, Santiago Daniel; et al.; Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy; Elsevier Science; International Journal Of Pharmaceutics; 455; 1-2; 8-2013; 48-56
0378-5173
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24159
identifier_str_mv Calles, Javier Adrián; Tartara, Luis Ignacio; Lopez Garcia, Alberto Raul; Diebold Luque, Yolanda; Palma, Santiago Daniel; et al.; Novel bioadhesive hyaluronan-itaconic acid crosslinked films for ocular therapy; Elsevier Science; International Journal Of Pharmaceutics; 455; 1-2; 8-2013; 48-56
0378-5173
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpharm.2013.07.063
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378517313006893
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269900607848448
score 13.13397

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