Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture
- Autores
- Tapia, Maria Ofelia; Stern, Marshall D.; Soraci, Alejandro Luis; Meronuck, Richard; Olson, William G.; Gold, Sandra; Koski-Hulbert, R.L.; Murphy, Michael J.
- Año de publicación
- 2005
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The objectives were to investigate the presence of patulin-producing Penicillium sp. in corn silage and high moisture corn as well as adverse effects of patulin on microbial fermentation in continuous culture fermenters. Eighty-three samples of corn silage or high moisture corn were cultured to determine the presence of molds. Penicillium sp. were isolated from 0.82 of samples. Of these Penicillium sp. isolates, 0.03 produced patulin on yeast extract sucrose and potato dextrose agar. The patulin-producing isolates belonged to the P. viridicatum group. The other molds identified were: Mucor sp. (0.45), Aspergillus sp. (0.41), and Fusarium sp. (0.25). Eight single-flow continuous culture fermenters were used to study effects of patulin on fermentation by ruminal microbes. Two 1-l fermenters were supplemented with 0, 10, 20 or 40 mg of patulin every 12 h for three consecutive days. Increasing patulin reduced neutral detergent and acid detergent fiber digestibility at a decreasing rate (linear, P < 0.01; quadratic, P < 0.05). True digestion of organic matter and total non-structural carbohydrates decreased linearly (P < 0.05) as patulin concentration increased. Crude protein digestion and bacterial N flows decreased linearly (P < 0.05). Conversely, there was a linear increase (P < 0.05) in ammonia nitrogen with increased patulin. Total, ammonia and non-ammonia N flows were not affected by patulin. Efficiency of microbial protein synthesis was not affected by patulin but there was a linear decreased (P < 0.05) in the efficiency of N utilization. Increasing patulin levels caused a linear decrease (P < 0.001) of total volatile fatty acid concentration and a quadratic decrease of acetate and propionate molar proportions (P < 0.05). Ten and 20 mg/l of patulin produced a decrease in acetate proportion and an increase in propionate proportion. Lactate concentration (mmol/l) increase from 0.0 to 216.5 mmol/l (linear, P < 0.05) with increasing patulin concentration. Penicillium sp. molds are common contaminants of corn silage and high moisture corn and they produce patulin that can adversely affect fermentation by ruminal microbes. Alterations in microbial digestion of dry matter, and production of microbial end products, impact the production and/or health of ruminants.
Fil: Tapia, Maria Ofelia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Stern, Marshall D.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Meronuck, Richard. University of Minnesota. Department of Plant Pathology; Estados Unidos
Fil: Olson, William G.. University of Minnesota. Department of Population Medicine; Estados Unidos
Fil: Gold, Sandra. University of Minnesota. Department of Plant Pathology; Estados Unidos
Fil: Koski-Hulbert, R.L.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Murphy, Michael J.. University of Minnesota. Department of Population Medicine; Estados Unidos - Materia
-
PATULIN
RUMEN
IN VITRO
RUMINAL FERMENTETION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/123056
Ver los metadatos del registro completo
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Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous cultureTapia, Maria OfeliaStern, Marshall D.Soraci, Alejandro LuisMeronuck, RichardOlson, William G.Gold, SandraKoski-Hulbert, R.L.Murphy, Michael J.PATULINRUMENIN VITRORUMINAL FERMENTETIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The objectives were to investigate the presence of patulin-producing Penicillium sp. in corn silage and high moisture corn as well as adverse effects of patulin on microbial fermentation in continuous culture fermenters. Eighty-three samples of corn silage or high moisture corn were cultured to determine the presence of molds. Penicillium sp. were isolated from 0.82 of samples. Of these Penicillium sp. isolates, 0.03 produced patulin on yeast extract sucrose and potato dextrose agar. The patulin-producing isolates belonged to the P. viridicatum group. The other molds identified were: Mucor sp. (0.45), Aspergillus sp. (0.41), and Fusarium sp. (0.25). Eight single-flow continuous culture fermenters were used to study effects of patulin on fermentation by ruminal microbes. Two 1-l fermenters were supplemented with 0, 10, 20 or 40 mg of patulin every 12 h for three consecutive days. Increasing patulin reduced neutral detergent and acid detergent fiber digestibility at a decreasing rate (linear, P < 0.01; quadratic, P < 0.05). True digestion of organic matter and total non-structural carbohydrates decreased linearly (P < 0.05) as patulin concentration increased. Crude protein digestion and bacterial N flows decreased linearly (P < 0.05). Conversely, there was a linear increase (P < 0.05) in ammonia nitrogen with increased patulin. Total, ammonia and non-ammonia N flows were not affected by patulin. Efficiency of microbial protein synthesis was not affected by patulin but there was a linear decreased (P < 0.05) in the efficiency of N utilization. Increasing patulin levels caused a linear decrease (P < 0.001) of total volatile fatty acid concentration and a quadratic decrease of acetate and propionate molar proportions (P < 0.05). Ten and 20 mg/l of patulin produced a decrease in acetate proportion and an increase in propionate proportion. Lactate concentration (mmol/l) increase from 0.0 to 216.5 mmol/l (linear, P < 0.05) with increasing patulin concentration. Penicillium sp. molds are common contaminants of corn silage and high moisture corn and they produce patulin that can adversely affect fermentation by ruminal microbes. Alterations in microbial digestion of dry matter, and production of microbial end products, impact the production and/or health of ruminants.Fil: Tapia, Maria Ofelia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Stern, Marshall D.. University of Minnesota. Department of Animal Science; Estados UnidosFil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Meronuck, Richard. University of Minnesota. Department of Plant Pathology; Estados UnidosFil: Olson, William G.. University of Minnesota. Department of Population Medicine; Estados UnidosFil: Gold, Sandra. University of Minnesota. Department of Plant Pathology; Estados UnidosFil: Koski-Hulbert, R.L.. University of Minnesota. Department of Animal Science; Estados UnidosFil: Murphy, Michael J.. University of Minnesota. Department of Population Medicine; Estados UnidosElsevier Science2005-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123056Tapia, Maria Ofelia; Stern, Marshall D.; Soraci, Alejandro Luis; Meronuck, Richard; Olson, William G.; et al.; Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture; Elsevier Science; Animal Feed Science and Technology; 119; 3-4; 4-2005; 247-2580377-8401CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anifeedsci.2004.12.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0377840104003037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:59Zoai:ri.conicet.gov.ar:11336/123056instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:59.896CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture |
title |
Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture |
spellingShingle |
Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture Tapia, Maria Ofelia PATULIN RUMEN IN VITRO RUMINAL FERMENTETION |
title_short |
Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture |
title_full |
Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture |
title_fullStr |
Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture |
title_full_unstemmed |
Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture |
title_sort |
Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture |
dc.creator.none.fl_str_mv |
Tapia, Maria Ofelia Stern, Marshall D. Soraci, Alejandro Luis Meronuck, Richard Olson, William G. Gold, Sandra Koski-Hulbert, R.L. Murphy, Michael J. |
author |
Tapia, Maria Ofelia |
author_facet |
Tapia, Maria Ofelia Stern, Marshall D. Soraci, Alejandro Luis Meronuck, Richard Olson, William G. Gold, Sandra Koski-Hulbert, R.L. Murphy, Michael J. |
author_role |
author |
author2 |
Stern, Marshall D. Soraci, Alejandro Luis Meronuck, Richard Olson, William G. Gold, Sandra Koski-Hulbert, R.L. Murphy, Michael J. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
PATULIN RUMEN IN VITRO RUMINAL FERMENTETION |
topic |
PATULIN RUMEN IN VITRO RUMINAL FERMENTETION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The objectives were to investigate the presence of patulin-producing Penicillium sp. in corn silage and high moisture corn as well as adverse effects of patulin on microbial fermentation in continuous culture fermenters. Eighty-three samples of corn silage or high moisture corn were cultured to determine the presence of molds. Penicillium sp. were isolated from 0.82 of samples. Of these Penicillium sp. isolates, 0.03 produced patulin on yeast extract sucrose and potato dextrose agar. The patulin-producing isolates belonged to the P. viridicatum group. The other molds identified were: Mucor sp. (0.45), Aspergillus sp. (0.41), and Fusarium sp. (0.25). Eight single-flow continuous culture fermenters were used to study effects of patulin on fermentation by ruminal microbes. Two 1-l fermenters were supplemented with 0, 10, 20 or 40 mg of patulin every 12 h for three consecutive days. Increasing patulin reduced neutral detergent and acid detergent fiber digestibility at a decreasing rate (linear, P < 0.01; quadratic, P < 0.05). True digestion of organic matter and total non-structural carbohydrates decreased linearly (P < 0.05) as patulin concentration increased. Crude protein digestion and bacterial N flows decreased linearly (P < 0.05). Conversely, there was a linear increase (P < 0.05) in ammonia nitrogen with increased patulin. Total, ammonia and non-ammonia N flows were not affected by patulin. Efficiency of microbial protein synthesis was not affected by patulin but there was a linear decreased (P < 0.05) in the efficiency of N utilization. Increasing patulin levels caused a linear decrease (P < 0.001) of total volatile fatty acid concentration and a quadratic decrease of acetate and propionate molar proportions (P < 0.05). Ten and 20 mg/l of patulin produced a decrease in acetate proportion and an increase in propionate proportion. Lactate concentration (mmol/l) increase from 0.0 to 216.5 mmol/l (linear, P < 0.05) with increasing patulin concentration. Penicillium sp. molds are common contaminants of corn silage and high moisture corn and they produce patulin that can adversely affect fermentation by ruminal microbes. Alterations in microbial digestion of dry matter, and production of microbial end products, impact the production and/or health of ruminants. Fil: Tapia, Maria Ofelia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Stern, Marshall D.. University of Minnesota. Department of Animal Science; Estados Unidos Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Meronuck, Richard. University of Minnesota. Department of Plant Pathology; Estados Unidos Fil: Olson, William G.. University of Minnesota. Department of Population Medicine; Estados Unidos Fil: Gold, Sandra. University of Minnesota. Department of Plant Pathology; Estados Unidos Fil: Koski-Hulbert, R.L.. University of Minnesota. Department of Animal Science; Estados Unidos Fil: Murphy, Michael J.. University of Minnesota. Department of Population Medicine; Estados Unidos |
description |
The objectives were to investigate the presence of patulin-producing Penicillium sp. in corn silage and high moisture corn as well as adverse effects of patulin on microbial fermentation in continuous culture fermenters. Eighty-three samples of corn silage or high moisture corn were cultured to determine the presence of molds. Penicillium sp. were isolated from 0.82 of samples. Of these Penicillium sp. isolates, 0.03 produced patulin on yeast extract sucrose and potato dextrose agar. The patulin-producing isolates belonged to the P. viridicatum group. The other molds identified were: Mucor sp. (0.45), Aspergillus sp. (0.41), and Fusarium sp. (0.25). Eight single-flow continuous culture fermenters were used to study effects of patulin on fermentation by ruminal microbes. Two 1-l fermenters were supplemented with 0, 10, 20 or 40 mg of patulin every 12 h for three consecutive days. Increasing patulin reduced neutral detergent and acid detergent fiber digestibility at a decreasing rate (linear, P < 0.01; quadratic, P < 0.05). True digestion of organic matter and total non-structural carbohydrates decreased linearly (P < 0.05) as patulin concentration increased. Crude protein digestion and bacterial N flows decreased linearly (P < 0.05). Conversely, there was a linear increase (P < 0.05) in ammonia nitrogen with increased patulin. Total, ammonia and non-ammonia N flows were not affected by patulin. Efficiency of microbial protein synthesis was not affected by patulin but there was a linear decreased (P < 0.05) in the efficiency of N utilization. Increasing patulin levels caused a linear decrease (P < 0.001) of total volatile fatty acid concentration and a quadratic decrease of acetate and propionate molar proportions (P < 0.05). Ten and 20 mg/l of patulin produced a decrease in acetate proportion and an increase in propionate proportion. Lactate concentration (mmol/l) increase from 0.0 to 216.5 mmol/l (linear, P < 0.05) with increasing patulin concentration. Penicillium sp. molds are common contaminants of corn silage and high moisture corn and they produce patulin that can adversely affect fermentation by ruminal microbes. Alterations in microbial digestion of dry matter, and production of microbial end products, impact the production and/or health of ruminants. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/123056 Tapia, Maria Ofelia; Stern, Marshall D.; Soraci, Alejandro Luis; Meronuck, Richard; Olson, William G.; et al.; Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture; Elsevier Science; Animal Feed Science and Technology; 119; 3-4; 4-2005; 247-258 0377-8401 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/123056 |
identifier_str_mv |
Tapia, Maria Ofelia; Stern, Marshall D.; Soraci, Alejandro Luis; Meronuck, Richard; Olson, William G.; et al.; Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture; Elsevier Science; Animal Feed Science and Technology; 119; 3-4; 4-2005; 247-258 0377-8401 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anifeedsci.2004.12.002 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0377840104003037 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269831090405376 |
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13.13397 |