Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture

Autores
Tapia, Maria Ofelia; Stern, Marshall D.; Soraci, Alejandro Luis; Meronuck, Richard; Olson, William G.; Gold, Sandra; Koski-Hulbert, R.L.; Murphy, Michael J.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objectives were to investigate the presence of patulin-producing Penicillium sp. in corn silage and high moisture corn as well as adverse effects of patulin on microbial fermentation in continuous culture fermenters. Eighty-three samples of corn silage or high moisture corn were cultured to determine the presence of molds. Penicillium sp. were isolated from 0.82 of samples. Of these Penicillium sp. isolates, 0.03 produced patulin on yeast extract sucrose and potato dextrose agar. The patulin-producing isolates belonged to the P. viridicatum group. The other molds identified were: Mucor sp. (0.45), Aspergillus sp. (0.41), and Fusarium sp. (0.25). Eight single-flow continuous culture fermenters were used to study effects of patulin on fermentation by ruminal microbes. Two 1-l fermenters were supplemented with 0, 10, 20 or 40 mg of patulin every 12 h for three consecutive days. Increasing patulin reduced neutral detergent and acid detergent fiber digestibility at a decreasing rate (linear, P < 0.01; quadratic, P < 0.05). True digestion of organic matter and total non-structural carbohydrates decreased linearly (P < 0.05) as patulin concentration increased. Crude protein digestion and bacterial N flows decreased linearly (P < 0.05). Conversely, there was a linear increase (P < 0.05) in ammonia nitrogen with increased patulin. Total, ammonia and non-ammonia N flows were not affected by patulin. Efficiency of microbial protein synthesis was not affected by patulin but there was a linear decreased (P < 0.05) in the efficiency of N utilization. Increasing patulin levels caused a linear decrease (P < 0.001) of total volatile fatty acid concentration and a quadratic decrease of acetate and propionate molar proportions (P < 0.05). Ten and 20 mg/l of patulin produced a decrease in acetate proportion and an increase in propionate proportion. Lactate concentration (mmol/l) increase from 0.0 to 216.5 mmol/l (linear, P < 0.05) with increasing patulin concentration. Penicillium sp. molds are common contaminants of corn silage and high moisture corn and they produce patulin that can adversely affect fermentation by ruminal microbes. Alterations in microbial digestion of dry matter, and production of microbial end products, impact the production and/or health of ruminants.
Fil: Tapia, Maria Ofelia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Stern, Marshall D.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Meronuck, Richard. University of Minnesota. Department of Plant Pathology; Estados Unidos
Fil: Olson, William G.. University of Minnesota. Department of Population Medicine; Estados Unidos
Fil: Gold, Sandra. University of Minnesota. Department of Plant Pathology; Estados Unidos
Fil: Koski-Hulbert, R.L.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Murphy, Michael J.. University of Minnesota. Department of Population Medicine; Estados Unidos
Materia
PATULIN
RUMEN
IN VITRO
RUMINAL FERMENTETION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123056

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network_name_str CONICET Digital (CONICET)
spelling Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous cultureTapia, Maria OfeliaStern, Marshall D.Soraci, Alejandro LuisMeronuck, RichardOlson, William G.Gold, SandraKoski-Hulbert, R.L.Murphy, Michael J.PATULINRUMENIN VITRORUMINAL FERMENTETIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The objectives were to investigate the presence of patulin-producing Penicillium sp. in corn silage and high moisture corn as well as adverse effects of patulin on microbial fermentation in continuous culture fermenters. Eighty-three samples of corn silage or high moisture corn were cultured to determine the presence of molds. Penicillium sp. were isolated from 0.82 of samples. Of these Penicillium sp. isolates, 0.03 produced patulin on yeast extract sucrose and potato dextrose agar. The patulin-producing isolates belonged to the P. viridicatum group. The other molds identified were: Mucor sp. (0.45), Aspergillus sp. (0.41), and Fusarium sp. (0.25). Eight single-flow continuous culture fermenters were used to study effects of patulin on fermentation by ruminal microbes. Two 1-l fermenters were supplemented with 0, 10, 20 or 40 mg of patulin every 12 h for three consecutive days. Increasing patulin reduced neutral detergent and acid detergent fiber digestibility at a decreasing rate (linear, P < 0.01; quadratic, P < 0.05). True digestion of organic matter and total non-structural carbohydrates decreased linearly (P < 0.05) as patulin concentration increased. Crude protein digestion and bacterial N flows decreased linearly (P < 0.05). Conversely, there was a linear increase (P < 0.05) in ammonia nitrogen with increased patulin. Total, ammonia and non-ammonia N flows were not affected by patulin. Efficiency of microbial protein synthesis was not affected by patulin but there was a linear decreased (P < 0.05) in the efficiency of N utilization. Increasing patulin levels caused a linear decrease (P < 0.001) of total volatile fatty acid concentration and a quadratic decrease of acetate and propionate molar proportions (P < 0.05). Ten and 20 mg/l of patulin produced a decrease in acetate proportion and an increase in propionate proportion. Lactate concentration (mmol/l) increase from 0.0 to 216.5 mmol/l (linear, P < 0.05) with increasing patulin concentration. Penicillium sp. molds are common contaminants of corn silage and high moisture corn and they produce patulin that can adversely affect fermentation by ruminal microbes. Alterations in microbial digestion of dry matter, and production of microbial end products, impact the production and/or health of ruminants.Fil: Tapia, Maria Ofelia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Stern, Marshall D.. University of Minnesota. Department of Animal Science; Estados UnidosFil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Meronuck, Richard. University of Minnesota. Department of Plant Pathology; Estados UnidosFil: Olson, William G.. University of Minnesota. Department of Population Medicine; Estados UnidosFil: Gold, Sandra. University of Minnesota. Department of Plant Pathology; Estados UnidosFil: Koski-Hulbert, R.L.. University of Minnesota. Department of Animal Science; Estados UnidosFil: Murphy, Michael J.. University of Minnesota. Department of Population Medicine; Estados UnidosElsevier Science2005-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123056Tapia, Maria Ofelia; Stern, Marshall D.; Soraci, Alejandro Luis; Meronuck, Richard; Olson, William G.; et al.; Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture; Elsevier Science; Animal Feed Science and Technology; 119; 3-4; 4-2005; 247-2580377-8401CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anifeedsci.2004.12.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0377840104003037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:59Zoai:ri.conicet.gov.ar:11336/123056instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:59.896CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture
title Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture
spellingShingle Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture
Tapia, Maria Ofelia
PATULIN
RUMEN
IN VITRO
RUMINAL FERMENTETION
title_short Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture
title_full Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture
title_fullStr Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture
title_full_unstemmed Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture
title_sort Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture
dc.creator.none.fl_str_mv Tapia, Maria Ofelia
Stern, Marshall D.
Soraci, Alejandro Luis
Meronuck, Richard
Olson, William G.
Gold, Sandra
Koski-Hulbert, R.L.
Murphy, Michael J.
author Tapia, Maria Ofelia
author_facet Tapia, Maria Ofelia
Stern, Marshall D.
Soraci, Alejandro Luis
Meronuck, Richard
Olson, William G.
Gold, Sandra
Koski-Hulbert, R.L.
Murphy, Michael J.
author_role author
author2 Stern, Marshall D.
Soraci, Alejandro Luis
Meronuck, Richard
Olson, William G.
Gold, Sandra
Koski-Hulbert, R.L.
Murphy, Michael J.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PATULIN
RUMEN
IN VITRO
RUMINAL FERMENTETION
topic PATULIN
RUMEN
IN VITRO
RUMINAL FERMENTETION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objectives were to investigate the presence of patulin-producing Penicillium sp. in corn silage and high moisture corn as well as adverse effects of patulin on microbial fermentation in continuous culture fermenters. Eighty-three samples of corn silage or high moisture corn were cultured to determine the presence of molds. Penicillium sp. were isolated from 0.82 of samples. Of these Penicillium sp. isolates, 0.03 produced patulin on yeast extract sucrose and potato dextrose agar. The patulin-producing isolates belonged to the P. viridicatum group. The other molds identified were: Mucor sp. (0.45), Aspergillus sp. (0.41), and Fusarium sp. (0.25). Eight single-flow continuous culture fermenters were used to study effects of patulin on fermentation by ruminal microbes. Two 1-l fermenters were supplemented with 0, 10, 20 or 40 mg of patulin every 12 h for three consecutive days. Increasing patulin reduced neutral detergent and acid detergent fiber digestibility at a decreasing rate (linear, P < 0.01; quadratic, P < 0.05). True digestion of organic matter and total non-structural carbohydrates decreased linearly (P < 0.05) as patulin concentration increased. Crude protein digestion and bacterial N flows decreased linearly (P < 0.05). Conversely, there was a linear increase (P < 0.05) in ammonia nitrogen with increased patulin. Total, ammonia and non-ammonia N flows were not affected by patulin. Efficiency of microbial protein synthesis was not affected by patulin but there was a linear decreased (P < 0.05) in the efficiency of N utilization. Increasing patulin levels caused a linear decrease (P < 0.001) of total volatile fatty acid concentration and a quadratic decrease of acetate and propionate molar proportions (P < 0.05). Ten and 20 mg/l of patulin produced a decrease in acetate proportion and an increase in propionate proportion. Lactate concentration (mmol/l) increase from 0.0 to 216.5 mmol/l (linear, P < 0.05) with increasing patulin concentration. Penicillium sp. molds are common contaminants of corn silage and high moisture corn and they produce patulin that can adversely affect fermentation by ruminal microbes. Alterations in microbial digestion of dry matter, and production of microbial end products, impact the production and/or health of ruminants.
Fil: Tapia, Maria Ofelia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Stern, Marshall D.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Meronuck, Richard. University of Minnesota. Department of Plant Pathology; Estados Unidos
Fil: Olson, William G.. University of Minnesota. Department of Population Medicine; Estados Unidos
Fil: Gold, Sandra. University of Minnesota. Department of Plant Pathology; Estados Unidos
Fil: Koski-Hulbert, R.L.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Murphy, Michael J.. University of Minnesota. Department of Population Medicine; Estados Unidos
description The objectives were to investigate the presence of patulin-producing Penicillium sp. in corn silage and high moisture corn as well as adverse effects of patulin on microbial fermentation in continuous culture fermenters. Eighty-three samples of corn silage or high moisture corn were cultured to determine the presence of molds. Penicillium sp. were isolated from 0.82 of samples. Of these Penicillium sp. isolates, 0.03 produced patulin on yeast extract sucrose and potato dextrose agar. The patulin-producing isolates belonged to the P. viridicatum group. The other molds identified were: Mucor sp. (0.45), Aspergillus sp. (0.41), and Fusarium sp. (0.25). Eight single-flow continuous culture fermenters were used to study effects of patulin on fermentation by ruminal microbes. Two 1-l fermenters were supplemented with 0, 10, 20 or 40 mg of patulin every 12 h for three consecutive days. Increasing patulin reduced neutral detergent and acid detergent fiber digestibility at a decreasing rate (linear, P < 0.01; quadratic, P < 0.05). True digestion of organic matter and total non-structural carbohydrates decreased linearly (P < 0.05) as patulin concentration increased. Crude protein digestion and bacterial N flows decreased linearly (P < 0.05). Conversely, there was a linear increase (P < 0.05) in ammonia nitrogen with increased patulin. Total, ammonia and non-ammonia N flows were not affected by patulin. Efficiency of microbial protein synthesis was not affected by patulin but there was a linear decreased (P < 0.05) in the efficiency of N utilization. Increasing patulin levels caused a linear decrease (P < 0.001) of total volatile fatty acid concentration and a quadratic decrease of acetate and propionate molar proportions (P < 0.05). Ten and 20 mg/l of patulin produced a decrease in acetate proportion and an increase in propionate proportion. Lactate concentration (mmol/l) increase from 0.0 to 216.5 mmol/l (linear, P < 0.05) with increasing patulin concentration. Penicillium sp. molds are common contaminants of corn silage and high moisture corn and they produce patulin that can adversely affect fermentation by ruminal microbes. Alterations in microbial digestion of dry matter, and production of microbial end products, impact the production and/or health of ruminants.
publishDate 2005
dc.date.none.fl_str_mv 2005-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123056
Tapia, Maria Ofelia; Stern, Marshall D.; Soraci, Alejandro Luis; Meronuck, Richard; Olson, William G.; et al.; Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture; Elsevier Science; Animal Feed Science and Technology; 119; 3-4; 4-2005; 247-258
0377-8401
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123056
identifier_str_mv Tapia, Maria Ofelia; Stern, Marshall D.; Soraci, Alejandro Luis; Meronuck, Richard; Olson, William G.; et al.; Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture; Elsevier Science; Animal Feed Science and Technology; 119; 3-4; 4-2005; 247-258
0377-8401
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0377840104003037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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