Effects of patulin on rumen microbial fermentation in continuous culture fermenters

Autores
Tapia, Maria Ofelia; Stern, Marsahll D.; Koski, R.L.; Bach, Alex; Murphy, Michael J.
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Eight single-flow continuous culture fermenters were used to study the effects of different concentrations of patulin on rumen microbial fermentation. Two 1 l fermenters were spiked with 0, 30, 60 or 90 mg of patulin every 12 h for 3 consecutive days. True digestion of organic matter (TOM), acid detergent fiber (ADF) and crude protein (CP) decreased (P<0.05, P<0.01 and P<0.01, respectively) with patulin addition ranging from 30 to 90 mg. Bacterial nitrogen flow was lower (P<0.01) with patulin addition (30–90 mg) compared with the control treatment, whereas, the efficiency of bacterial growth (g of N/kg OMTD) was lowest (P<0.05) when 90 mg of patulin were added to fermenter flasks. Total volatile fatty acid (VFA) concentration (mM) in fermenter effluents decreased (P<0.05) from 180.1 to 119.5 with the addition of 90 mg of patulin but did not differ (P>0.05) between the control treatment and 30–60 mg of patulin. Acetate (mol/100 mol) was depressed (P<0.01) with patulin addition. Conversely, there was an increase (P<0.05) in the molar proportion of butyrate and valerate in the fermenters treated with the toxin. At the highest level of patulin addition (60 and 90 mg), branched-chain VFA were lower (P<0.01) probably due to a reduction in protein (branched-chain amino acid) degradation. It is concluded that patulin can alter metabolism of nutrients by ruminal microbes. These changes could potentially have a negative impact on animal health and performance.
Fil: Tapia, Maria Ofelia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Stern, Marsahll D.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Koski, R.L.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Bach, Alex. Agribrands Europa; España
Fil: Murphy, Michael J.. University of Minnesota. Department of Veterinary Diagnostic Medicine; Estados Unidos
Materia
PATULIN
RUMEN
FERMENTATION
IN VITRO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123048
Acceder
id CONICETDig_accc7a76e2f537fe8f285791371fc731
oai_identifier_str oai:ri.conicet.gov.ar:11336/123048
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of patulin on rumen microbial fermentation in continuous culture fermentersTapia, Maria OfeliaStern, Marsahll D.Koski, R.L.Bach, AlexMurphy, Michael J.PATULINRUMENFERMENTATIONIN VITROhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Eight single-flow continuous culture fermenters were used to study the effects of different concentrations of patulin on rumen microbial fermentation. Two 1 l fermenters were spiked with 0, 30, 60 or 90 mg of patulin every 12 h for 3 consecutive days. True digestion of organic matter (TOM), acid detergent fiber (ADF) and crude protein (CP) decreased (P<0.05, P<0.01 and P<0.01, respectively) with patulin addition ranging from 30 to 90 mg. Bacterial nitrogen flow was lower (P<0.01) with patulin addition (30–90 mg) compared with the control treatment, whereas, the efficiency of bacterial growth (g of N/kg OMTD) was lowest (P<0.05) when 90 mg of patulin were added to fermenter flasks. Total volatile fatty acid (VFA) concentration (mM) in fermenter effluents decreased (P<0.05) from 180.1 to 119.5 with the addition of 90 mg of patulin but did not differ (P>0.05) between the control treatment and 30–60 mg of patulin. Acetate (mol/100 mol) was depressed (P<0.01) with patulin addition. Conversely, there was an increase (P<0.05) in the molar proportion of butyrate and valerate in the fermenters treated with the toxin. At the highest level of patulin addition (60 and 90 mg), branched-chain VFA were lower (P<0.01) probably due to a reduction in protein (branched-chain amino acid) degradation. It is concluded that patulin can alter metabolism of nutrients by ruminal microbes. These changes could potentially have a negative impact on animal health and performance.Fil: Tapia, Maria Ofelia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Stern, Marsahll D.. University of Minnesota. Department of Animal Science; Estados UnidosFil: Koski, R.L.. University of Minnesota. Department of Animal Science; Estados UnidosFil: Bach, Alex. Agribrands Europa; EspañaFil: Murphy, Michael J.. University of Minnesota. Department of Veterinary Diagnostic Medicine; Estados UnidosElsevier Science2002-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123048Tapia, Maria Ofelia; Stern, Marsahll D.; Koski, R.L.; Bach, Alex; Murphy, Michael J.; Effects of patulin on rumen microbial fermentation in continuous culture fermenters; Elsevier Science; Animal Feed Science and Technology; 97; 3-4; 6-2002; 239-2460377-84011873-2216CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0377-8401(02)00007-Xinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S037784010200007Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:21Zoai:ri.conicet.gov.ar:11336/123048instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:21.577CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of patulin on rumen microbial fermentation in continuous culture fermenters
title Effects of patulin on rumen microbial fermentation in continuous culture fermenters
spellingShingle Effects of patulin on rumen microbial fermentation in continuous culture fermenters
Tapia, Maria Ofelia
PATULIN
RUMEN
FERMENTATION
IN VITRO
title_short Effects of patulin on rumen microbial fermentation in continuous culture fermenters
title_full Effects of patulin on rumen microbial fermentation in continuous culture fermenters
title_fullStr Effects of patulin on rumen microbial fermentation in continuous culture fermenters
title_full_unstemmed Effects of patulin on rumen microbial fermentation in continuous culture fermenters
title_sort Effects of patulin on rumen microbial fermentation in continuous culture fermenters
dc.creator.none.fl_str_mv Tapia, Maria Ofelia
Stern, Marsahll D.
Koski, R.L.
Bach, Alex
Murphy, Michael J.
author Tapia, Maria Ofelia
author_facet Tapia, Maria Ofelia
Stern, Marsahll D.
Koski, R.L.
Bach, Alex
Murphy, Michael J.
author_role author
author2 Stern, Marsahll D.
Koski, R.L.
Bach, Alex
Murphy, Michael J.
author2_role author
author
author
author
dc.subject.none.fl_str_mv PATULIN
RUMEN
FERMENTATION
IN VITRO
topic PATULIN
RUMEN
FERMENTATION
IN VITRO
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Eight single-flow continuous culture fermenters were used to study the effects of different concentrations of patulin on rumen microbial fermentation. Two 1 l fermenters were spiked with 0, 30, 60 or 90 mg of patulin every 12 h for 3 consecutive days. True digestion of organic matter (TOM), acid detergent fiber (ADF) and crude protein (CP) decreased (P<0.05, P<0.01 and P<0.01, respectively) with patulin addition ranging from 30 to 90 mg. Bacterial nitrogen flow was lower (P<0.01) with patulin addition (30–90 mg) compared with the control treatment, whereas, the efficiency of bacterial growth (g of N/kg OMTD) was lowest (P<0.05) when 90 mg of patulin were added to fermenter flasks. Total volatile fatty acid (VFA) concentration (mM) in fermenter effluents decreased (P<0.05) from 180.1 to 119.5 with the addition of 90 mg of patulin but did not differ (P>0.05) between the control treatment and 30–60 mg of patulin. Acetate (mol/100 mol) was depressed (P<0.01) with patulin addition. Conversely, there was an increase (P<0.05) in the molar proportion of butyrate and valerate in the fermenters treated with the toxin. At the highest level of patulin addition (60 and 90 mg), branched-chain VFA were lower (P<0.01) probably due to a reduction in protein (branched-chain amino acid) degradation. It is concluded that patulin can alter metabolism of nutrients by ruminal microbes. These changes could potentially have a negative impact on animal health and performance.
Fil: Tapia, Maria Ofelia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Stern, Marsahll D.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Koski, R.L.. University of Minnesota. Department of Animal Science; Estados Unidos
Fil: Bach, Alex. Agribrands Europa; España
Fil: Murphy, Michael J.. University of Minnesota. Department of Veterinary Diagnostic Medicine; Estados Unidos
description Eight single-flow continuous culture fermenters were used to study the effects of different concentrations of patulin on rumen microbial fermentation. Two 1 l fermenters were spiked with 0, 30, 60 or 90 mg of patulin every 12 h for 3 consecutive days. True digestion of organic matter (TOM), acid detergent fiber (ADF) and crude protein (CP) decreased (P<0.05, P<0.01 and P<0.01, respectively) with patulin addition ranging from 30 to 90 mg. Bacterial nitrogen flow was lower (P<0.01) with patulin addition (30–90 mg) compared with the control treatment, whereas, the efficiency of bacterial growth (g of N/kg OMTD) was lowest (P<0.05) when 90 mg of patulin were added to fermenter flasks. Total volatile fatty acid (VFA) concentration (mM) in fermenter effluents decreased (P<0.05) from 180.1 to 119.5 with the addition of 90 mg of patulin but did not differ (P>0.05) between the control treatment and 30–60 mg of patulin. Acetate (mol/100 mol) was depressed (P<0.01) with patulin addition. Conversely, there was an increase (P<0.05) in the molar proportion of butyrate and valerate in the fermenters treated with the toxin. At the highest level of patulin addition (60 and 90 mg), branched-chain VFA were lower (P<0.01) probably due to a reduction in protein (branched-chain amino acid) degradation. It is concluded that patulin can alter metabolism of nutrients by ruminal microbes. These changes could potentially have a negative impact on animal health and performance.
publishDate 2002
dc.date.none.fl_str_mv 2002-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123048
Tapia, Maria Ofelia; Stern, Marsahll D.; Koski, R.L.; Bach, Alex; Murphy, Michael J.; Effects of patulin on rumen microbial fermentation in continuous culture fermenters; Elsevier Science; Animal Feed Science and Technology; 97; 3-4; 6-2002; 239-246
0377-8401
1873-2216
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123048
identifier_str_mv Tapia, Maria Ofelia; Stern, Marsahll D.; Koski, R.L.; Bach, Alex; Murphy, Michael J.; Effects of patulin on rumen microbial fermentation in continuous culture fermenters; Elsevier Science; Animal Feed Science and Technology; 97; 3-4; 6-2002; 239-246
0377-8401
1873-2216
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0377-8401(02)00007-X
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S037784010200007X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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