Lactococcus lactis is capable of improving the riboflavin status in deficient rats

Autores
Leblanc, Jean Guy Joseph; Burgess, Catherine; Sesma, Fernando Juan Manuel; Savoy, Graciela; van Sinderen, Douwe
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactococcus lactis is a commonly used starter strain that can be converted from a vitamin B2 consumer into a vitamin B2 'factory' by over-expressing its riboflavin biosynthesis genes. The present study was conducted to assess in a rat bioassay the response of riboflavin produced by GM or native lactic acid bacteria (LAB). The riboflavin-producing strains were able to eliminate most physiological manifestations of ariboflavinosis such as stunted growth, elevated erythrocyte glutathione reductase activation coefficient values and hepatomegalia that were observed using a riboflavin depletion-repletion model. Riboflavin status and growth rates were greatly improved when the depleted rats were fed with cultures of L. lactis that overproduced this vitamin whereas the native strain did not show the same effect. The present study is the first animal trial with food containing living bacteria that were engineered to overproduce riboflavin. These results pave the way for analysing the effect of similar riboflavin-overproducing LAB in human trials.
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Burgess, Catherine. University College Cork; Irlanda
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: van Sinderen, Douwe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Ariboflavinosis
Genetically Modified Micro-Organisms
Lactic Acid Bacteria
Riboflavin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55474
Acceder
id CONICETDig_e98112216aed284f6cc52b586b3a4a57
oai_identifier_str oai:ri.conicet.gov.ar:11336/55474
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactococcus lactis is capable of improving the riboflavin status in deficient ratsLeblanc, Jean Guy JosephBurgess, CatherineSesma, Fernando Juan ManuelSavoy, Gracielavan Sinderen, DouweAriboflavinosisGenetically Modified Micro-OrganismsLactic Acid BacteriaRiboflavinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactococcus lactis is a commonly used starter strain that can be converted from a vitamin B2 consumer into a vitamin B2 'factory' by over-expressing its riboflavin biosynthesis genes. The present study was conducted to assess in a rat bioassay the response of riboflavin produced by GM or native lactic acid bacteria (LAB). The riboflavin-producing strains were able to eliminate most physiological manifestations of ariboflavinosis such as stunted growth, elevated erythrocyte glutathione reductase activation coefficient values and hepatomegalia that were observed using a riboflavin depletion-repletion model. Riboflavin status and growth rates were greatly improved when the depleted rats were fed with cultures of L. lactis that overproduced this vitamin whereas the native strain did not show the same effect. The present study is the first animal trial with food containing living bacteria that were engineered to overproduce riboflavin. These results pave the way for analysing the effect of similar riboflavin-overproducing LAB in human trials.Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Burgess, Catherine. University College Cork; IrlandaFil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: van Sinderen, Douwe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaCambridge University Press2005-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55474Leblanc, Jean Guy Joseph; Burgess, Catherine; Sesma, Fernando Juan Manuel; Savoy, Graciela; van Sinderen, Douwe; Lactococcus lactis is capable of improving the riboflavin status in deficient rats; Cambridge University Press; British Journal of Nutrition; 94; 2; 8-2005; 262-2670007-1145CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1079/BJN20051473info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/lactococcus-lactis-is-capable-of-improving-the-riboflavin-status-in-deficient-rats/ED81D4E1A1235FB43081BB8B980D1444info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:25:00Zoai:ri.conicet.gov.ar:11336/55474instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:25:01.147CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactococcus lactis is capable of improving the riboflavin status in deficient rats
title Lactococcus lactis is capable of improving the riboflavin status in deficient rats
spellingShingle Lactococcus lactis is capable of improving the riboflavin status in deficient rats
Leblanc, Jean Guy Joseph
Ariboflavinosis
Genetically Modified Micro-Organisms
Lactic Acid Bacteria
Riboflavin
title_short Lactococcus lactis is capable of improving the riboflavin status in deficient rats
title_full Lactococcus lactis is capable of improving the riboflavin status in deficient rats
title_fullStr Lactococcus lactis is capable of improving the riboflavin status in deficient rats
title_full_unstemmed Lactococcus lactis is capable of improving the riboflavin status in deficient rats
title_sort Lactococcus lactis is capable of improving the riboflavin status in deficient rats
dc.creator.none.fl_str_mv Leblanc, Jean Guy Joseph
Burgess, Catherine
Sesma, Fernando Juan Manuel
Savoy, Graciela
van Sinderen, Douwe
author Leblanc, Jean Guy Joseph
author_facet Leblanc, Jean Guy Joseph
Burgess, Catherine
Sesma, Fernando Juan Manuel
Savoy, Graciela
van Sinderen, Douwe
author_role author
author2 Burgess, Catherine
Sesma, Fernando Juan Manuel
Savoy, Graciela
van Sinderen, Douwe
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ariboflavinosis
Genetically Modified Micro-Organisms
Lactic Acid Bacteria
Riboflavin
topic Ariboflavinosis
Genetically Modified Micro-Organisms
Lactic Acid Bacteria
Riboflavin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactococcus lactis is a commonly used starter strain that can be converted from a vitamin B2 consumer into a vitamin B2 'factory' by over-expressing its riboflavin biosynthesis genes. The present study was conducted to assess in a rat bioassay the response of riboflavin produced by GM or native lactic acid bacteria (LAB). The riboflavin-producing strains were able to eliminate most physiological manifestations of ariboflavinosis such as stunted growth, elevated erythrocyte glutathione reductase activation coefficient values and hepatomegalia that were observed using a riboflavin depletion-repletion model. Riboflavin status and growth rates were greatly improved when the depleted rats were fed with cultures of L. lactis that overproduced this vitamin whereas the native strain did not show the same effect. The present study is the first animal trial with food containing living bacteria that were engineered to overproduce riboflavin. These results pave the way for analysing the effect of similar riboflavin-overproducing LAB in human trials.
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Burgess, Catherine. University College Cork; Irlanda
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: van Sinderen, Douwe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Lactococcus lactis is a commonly used starter strain that can be converted from a vitamin B2 consumer into a vitamin B2 'factory' by over-expressing its riboflavin biosynthesis genes. The present study was conducted to assess in a rat bioassay the response of riboflavin produced by GM or native lactic acid bacteria (LAB). The riboflavin-producing strains were able to eliminate most physiological manifestations of ariboflavinosis such as stunted growth, elevated erythrocyte glutathione reductase activation coefficient values and hepatomegalia that were observed using a riboflavin depletion-repletion model. Riboflavin status and growth rates were greatly improved when the depleted rats were fed with cultures of L. lactis that overproduced this vitamin whereas the native strain did not show the same effect. The present study is the first animal trial with food containing living bacteria that were engineered to overproduce riboflavin. These results pave the way for analysing the effect of similar riboflavin-overproducing LAB in human trials.
publishDate 2005
dc.date.none.fl_str_mv 2005-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55474
Leblanc, Jean Guy Joseph; Burgess, Catherine; Sesma, Fernando Juan Manuel; Savoy, Graciela; van Sinderen, Douwe; Lactococcus lactis is capable of improving the riboflavin status in deficient rats; Cambridge University Press; British Journal of Nutrition; 94; 2; 8-2005; 262-267
0007-1145
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55474
identifier_str_mv Leblanc, Jean Guy Joseph; Burgess, Catherine; Sesma, Fernando Juan Manuel; Savoy, Graciela; van Sinderen, Douwe; Lactococcus lactis is capable of improving the riboflavin status in deficient rats; Cambridge University Press; British Journal of Nutrition; 94; 2; 8-2005; 262-267
0007-1145
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1079/BJN20051473
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/lactococcus-lactis-is-capable-of-improving-the-riboflavin-status-in-deficient-rats/ED81D4E1A1235FB43081BB8B980D1444
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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