Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats

Autores
Leblanc, Jean Guy Joseph; Sybesma, Wilbert; Starrenburg, Marjo; Sesma, Fernando Juan Manuel; de Vos, Willem M.; Savoy, Graciela; Hugenholtz, Jeroen
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to establish the bioavailability of different folates produced by engineered Lactococcus (L.) lactis strains using a rodent depletion-repletion bioassay. Research Methods & Procedures: Rats were fed a folate-deficient diet, which produces a reversible subclinical folate deficiency, supplemented with different L. lactis cultures which were added as the only source of folate. Three bacterial strains that over-expressed either the folC, folKE or folC+KE genes were used. These strains produce folates with different polyglutamyl tail lengths. The growth response of the rats and the concentration of folates in different organs and blood samples were monitored. Results: The folate produced by the engineered strains was able to compensate the folate depletion in the diet and showed similar bioavailability compared to commercial folic acid that is normally used for food fortification. Folate concentrations in organ and blood samples increased significantly in animals that received the folate producing strains compared to those that did not receive bacterial supplementation. Hematological studies also showed that the administration of the L. lactis strains was able to revert a partial megaloblastic anemia caused by folate deficiency. No significant differences were observed in bioavailability of folates containing different glutamyl tail lengths. Conclusions: To our knowledge, this is the first study that demonstrated that folates produced by engineered lactic acid bacteria represent a bioavailable source of this essential vitamin.
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sybesma, Wilbert. Wageningen Centre for Food Sciences; Países Bajos. Nizo Food Research; Países Bajos
Fil: Starrenburg, Marjo. Wageningen Centre for Food Sciences; Países Bajos. Nizo Food Research; Países Bajos
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Vos, Willem M.. Wageningen Centre for Food Sciences; Países Bajos
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hugenholtz, Jeroen. Nizo Food Research; Países Bajos. Wageningen Centre for Food Sciences; Países Bajos
Materia
FOLATES
GENETICALLY MODIFIED MICROORGANISMS
LACTOCOCCUS LACTIS
ANIMAL MODEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/279098
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/279098
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Supplementation with engineered Lactococcus lactis improves the folate status in deficient ratsLeblanc, Jean Guy JosephSybesma, WilbertStarrenburg, MarjoSesma, Fernando Juan Manuelde Vos, Willem M.Savoy, GracielaHugenholtz, JeroenFOLATESGENETICALLY MODIFIED MICROORGANISMSLACTOCOCCUS LACTISANIMAL MODELhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The aim of this study was to establish the bioavailability of different folates produced by engineered Lactococcus (L.) lactis strains using a rodent depletion-repletion bioassay. Research Methods & Procedures: Rats were fed a folate-deficient diet, which produces a reversible subclinical folate deficiency, supplemented with different L. lactis cultures which were added as the only source of folate. Three bacterial strains that over-expressed either the folC, folKE or folC+KE genes were used. These strains produce folates with different polyglutamyl tail lengths. The growth response of the rats and the concentration of folates in different organs and blood samples were monitored. Results: The folate produced by the engineered strains was able to compensate the folate depletion in the diet and showed similar bioavailability compared to commercial folic acid that is normally used for food fortification. Folate concentrations in organ and blood samples increased significantly in animals that received the folate producing strains compared to those that did not receive bacterial supplementation. Hematological studies also showed that the administration of the L. lactis strains was able to revert a partial megaloblastic anemia caused by folate deficiency. No significant differences were observed in bioavailability of folates containing different glutamyl tail lengths. Conclusions: To our knowledge, this is the first study that demonstrated that folates produced by engineered lactic acid bacteria represent a bioavailable source of this essential vitamin.Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Sybesma, Wilbert. Wageningen Centre for Food Sciences; Países Bajos. Nizo Food Research; Países BajosFil: Starrenburg, Marjo. Wageningen Centre for Food Sciences; Países Bajos. Nizo Food Research; Países BajosFil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: de Vos, Willem M.. Wageningen Centre for Food Sciences; Países BajosFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Hugenholtz, Jeroen. Nizo Food Research; Países Bajos. Wageningen Centre for Food Sciences; Países BajosElsevier Science Inc.2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/279098Leblanc, Jean Guy Joseph; Sybesma, Wilbert; Starrenburg, Marjo; Sesma, Fernando Juan Manuel; de Vos, Willem M.; et al.; Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats; Elsevier Science Inc.; Nutrition; 26; 7-8; 7-2010; 835-8410899-9007CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0899900709002986info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nut.2009.06.023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:03:45Zoai:ri.conicet.gov.ar:11336/279098instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:03:45.696CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats
title Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats
spellingShingle Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats
Leblanc, Jean Guy Joseph
FOLATES
GENETICALLY MODIFIED MICROORGANISMS
LACTOCOCCUS LACTIS
ANIMAL MODEL
title_short Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats
title_full Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats
title_fullStr Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats
title_full_unstemmed Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats
title_sort Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats
dc.creator.none.fl_str_mv Leblanc, Jean Guy Joseph
Sybesma, Wilbert
Starrenburg, Marjo
Sesma, Fernando Juan Manuel
de Vos, Willem M.
Savoy, Graciela
Hugenholtz, Jeroen
author Leblanc, Jean Guy Joseph
author_facet Leblanc, Jean Guy Joseph
Sybesma, Wilbert
Starrenburg, Marjo
Sesma, Fernando Juan Manuel
de Vos, Willem M.
Savoy, Graciela
Hugenholtz, Jeroen
author_role author
author2 Sybesma, Wilbert
Starrenburg, Marjo
Sesma, Fernando Juan Manuel
de Vos, Willem M.
Savoy, Graciela
Hugenholtz, Jeroen
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv FOLATES
GENETICALLY MODIFIED MICROORGANISMS
LACTOCOCCUS LACTIS
ANIMAL MODEL
topic FOLATES
GENETICALLY MODIFIED MICROORGANISMS
LACTOCOCCUS LACTIS
ANIMAL MODEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The aim of this study was to establish the bioavailability of different folates produced by engineered Lactococcus (L.) lactis strains using a rodent depletion-repletion bioassay. Research Methods & Procedures: Rats were fed a folate-deficient diet, which produces a reversible subclinical folate deficiency, supplemented with different L. lactis cultures which were added as the only source of folate. Three bacterial strains that over-expressed either the folC, folKE or folC+KE genes were used. These strains produce folates with different polyglutamyl tail lengths. The growth response of the rats and the concentration of folates in different organs and blood samples were monitored. Results: The folate produced by the engineered strains was able to compensate the folate depletion in the diet and showed similar bioavailability compared to commercial folic acid that is normally used for food fortification. Folate concentrations in organ and blood samples increased significantly in animals that received the folate producing strains compared to those that did not receive bacterial supplementation. Hematological studies also showed that the administration of the L. lactis strains was able to revert a partial megaloblastic anemia caused by folate deficiency. No significant differences were observed in bioavailability of folates containing different glutamyl tail lengths. Conclusions: To our knowledge, this is the first study that demonstrated that folates produced by engineered lactic acid bacteria represent a bioavailable source of this essential vitamin.
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sybesma, Wilbert. Wageningen Centre for Food Sciences; Países Bajos. Nizo Food Research; Países Bajos
Fil: Starrenburg, Marjo. Wageningen Centre for Food Sciences; Países Bajos. Nizo Food Research; Países Bajos
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Vos, Willem M.. Wageningen Centre for Food Sciences; Países Bajos
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hugenholtz, Jeroen. Nizo Food Research; Países Bajos. Wageningen Centre for Food Sciences; Países Bajos
description The aim of this study was to establish the bioavailability of different folates produced by engineered Lactococcus (L.) lactis strains using a rodent depletion-repletion bioassay. Research Methods & Procedures: Rats were fed a folate-deficient diet, which produces a reversible subclinical folate deficiency, supplemented with different L. lactis cultures which were added as the only source of folate. Three bacterial strains that over-expressed either the folC, folKE or folC+KE genes were used. These strains produce folates with different polyglutamyl tail lengths. The growth response of the rats and the concentration of folates in different organs and blood samples were monitored. Results: The folate produced by the engineered strains was able to compensate the folate depletion in the diet and showed similar bioavailability compared to commercial folic acid that is normally used for food fortification. Folate concentrations in organ and blood samples increased significantly in animals that received the folate producing strains compared to those that did not receive bacterial supplementation. Hematological studies also showed that the administration of the L. lactis strains was able to revert a partial megaloblastic anemia caused by folate deficiency. No significant differences were observed in bioavailability of folates containing different glutamyl tail lengths. Conclusions: To our knowledge, this is the first study that demonstrated that folates produced by engineered lactic acid bacteria represent a bioavailable source of this essential vitamin.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/279098
Leblanc, Jean Guy Joseph; Sybesma, Wilbert; Starrenburg, Marjo; Sesma, Fernando Juan Manuel; de Vos, Willem M.; et al.; Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats; Elsevier Science Inc.; Nutrition; 26; 7-8; 7-2010; 835-841
0899-9007
CONICET Digital
CONICET
url http://hdl.handle.net/11336/279098
identifier_str_mv Leblanc, Jean Guy Joseph; Sybesma, Wilbert; Starrenburg, Marjo; Sesma, Fernando Juan Manuel; de Vos, Willem M.; et al.; Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats; Elsevier Science Inc.; Nutrition; 26; 7-8; 7-2010; 835-841
0899-9007
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0899900709002986
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nut.2009.06.023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc.
publisher.none.fl_str_mv Elsevier Science Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.106097

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