First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)

Autores
Brihuega, Bibiana; Grune Loffler, Sylvia; Samartino, Luis Ernesto; Romero, Graciela; Auteri, Carmelo; Martínez, Mara
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosisis maintained in nature by chronic renal infection of carrier animals, being rodents andother small mammals the most important reservoirs. Also, significant sources of humaninfection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa)is an introduced species from Europe which is widespread in America and particularlyin the Center and South of Argentina. This species is highly valued by hunters in theregion and their meat is consumed more often. In many countries of the world (Europe,USA and Australia) studies of seroprevalence have been carried out, the seroreactivitydoes not mean that the wild boar has clinical symptoms of leptospirosis or that wildboars are maintenance host of this pathogen. However, these animals have been incontact to leptospiras in their environment in the past. This study has the objective tomolecularly genotype the pathogenic Leptospira sp. isolated strain obtained fromfetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wildboar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy wasperformed of the 4 fetuses and samples of kidneys, liver, spleen and lung werecultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung andkidney of fetuses were processed and analyzed by direct immunofluorescence.Multiple Locus Variable number tandem repeats Analysis (MLVA) was used tocharacterize the isolated strain. The genetic profile of the isolated pathogenicLeptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovarCastellonis Castellon 3, serogroup Ballum. To the best of our knowledge this is the firstisolation of Leptospira spp. from a wild boar fetus.
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Romero, Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Auteri, Carmelo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Martínez, Mara. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Materia
LEPTOSPIRA SPP
WILD BOAR
GENOTYPE
PATAGONIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/83370
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/83370
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)Brihuega, BibianaGrune Loffler, SylviaSamartino, Luis ErnestoRomero, GracielaAuteri, CarmeloMartínez, MaraLEPTOSPIRA SPPWILD BOARGENOTYPEPATAGONIAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosisis maintained in nature by chronic renal infection of carrier animals, being rodents andother small mammals the most important reservoirs. Also, significant sources of humaninfection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa)is an introduced species from Europe which is widespread in America and particularlyin the Center and South of Argentina. This species is highly valued by hunters in theregion and their meat is consumed more often. In many countries of the world (Europe,USA and Australia) studies of seroprevalence have been carried out, the seroreactivitydoes not mean that the wild boar has clinical symptoms of leptospirosis or that wildboars are maintenance host of this pathogen. However, these animals have been incontact to leptospiras in their environment in the past. This study has the objective tomolecularly genotype the pathogenic Leptospira sp. isolated strain obtained fromfetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wildboar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy wasperformed of the 4 fetuses and samples of kidneys, liver, spleen and lung werecultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung andkidney of fetuses were processed and analyzed by direct immunofluorescence.Multiple Locus Variable number tandem repeats Analysis (MLVA) was used tocharacterize the isolated strain. The genetic profile of the isolated pathogenicLeptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovarCastellonis Castellon 3, serogroup Ballum. To the best of our knowledge this is the firstisolation of Leptospira spp. from a wild boar fetus.Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Romero, Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Auteri, Carmelo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Martínez, Mara. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaiMed Pub2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/83370Brihuega, Bibiana; Grune Loffler, Sylvia; Samartino, Luis Ernesto; Romero, Graciela; Auteri, Carmelo; et al.; First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa); iMed Pub; Electronic Journal of Biology; 13; 1; 2-2017; 63-661860-3122CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ejbio.imedpub.com/first-isolation-of-leptospira-borgpetersenii-from-fetuses-ofwild-boars-sus-scrofa.php?aid=18235info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:02Zoai:ri.conicet.gov.ar:11336/83370instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:02.827CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)
title First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)
spellingShingle First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)
Brihuega, Bibiana
LEPTOSPIRA SPP
WILD BOAR
GENOTYPE
PATAGONIA
title_short First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)
title_full First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)
title_fullStr First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)
title_full_unstemmed First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)
title_sort First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa)
dc.creator.none.fl_str_mv Brihuega, Bibiana
Grune Loffler, Sylvia
Samartino, Luis Ernesto
Romero, Graciela
Auteri, Carmelo
Martínez, Mara
author Brihuega, Bibiana
author_facet Brihuega, Bibiana
Grune Loffler, Sylvia
Samartino, Luis Ernesto
Romero, Graciela
Auteri, Carmelo
Martínez, Mara
author_role author
author2 Grune Loffler, Sylvia
Samartino, Luis Ernesto
Romero, Graciela
Auteri, Carmelo
Martínez, Mara
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv LEPTOSPIRA SPP
WILD BOAR
GENOTYPE
PATAGONIA
topic LEPTOSPIRA SPP
WILD BOAR
GENOTYPE
PATAGONIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosisis maintained in nature by chronic renal infection of carrier animals, being rodents andother small mammals the most important reservoirs. Also, significant sources of humaninfection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa)is an introduced species from Europe which is widespread in America and particularlyin the Center and South of Argentina. This species is highly valued by hunters in theregion and their meat is consumed more often. In many countries of the world (Europe,USA and Australia) studies of seroprevalence have been carried out, the seroreactivitydoes not mean that the wild boar has clinical symptoms of leptospirosis or that wildboars are maintenance host of this pathogen. However, these animals have been incontact to leptospiras in their environment in the past. This study has the objective tomolecularly genotype the pathogenic Leptospira sp. isolated strain obtained fromfetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wildboar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy wasperformed of the 4 fetuses and samples of kidneys, liver, spleen and lung werecultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung andkidney of fetuses were processed and analyzed by direct immunofluorescence.Multiple Locus Variable number tandem repeats Analysis (MLVA) was used tocharacterize the isolated strain. The genetic profile of the isolated pathogenicLeptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovarCastellonis Castellon 3, serogroup Ballum. To the best of our knowledge this is the firstisolation of Leptospira spp. from a wild boar fetus.
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Romero, Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Auteri, Carmelo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Martínez, Mara. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
description Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosisis maintained in nature by chronic renal infection of carrier animals, being rodents andother small mammals the most important reservoirs. Also, significant sources of humaninfection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa)is an introduced species from Europe which is widespread in America and particularlyin the Center and South of Argentina. This species is highly valued by hunters in theregion and their meat is consumed more often. In many countries of the world (Europe,USA and Australia) studies of seroprevalence have been carried out, the seroreactivitydoes not mean that the wild boar has clinical symptoms of leptospirosis or that wildboars are maintenance host of this pathogen. However, these animals have been incontact to leptospiras in their environment in the past. This study has the objective tomolecularly genotype the pathogenic Leptospira sp. isolated strain obtained fromfetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wildboar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy wasperformed of the 4 fetuses and samples of kidneys, liver, spleen and lung werecultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung andkidney of fetuses were processed and analyzed by direct immunofluorescence.Multiple Locus Variable number tandem repeats Analysis (MLVA) was used tocharacterize the isolated strain. The genetic profile of the isolated pathogenicLeptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovarCastellonis Castellon 3, serogroup Ballum. To the best of our knowledge this is the firstisolation of Leptospira spp. from a wild boar fetus.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/83370
Brihuega, Bibiana; Grune Loffler, Sylvia; Samartino, Luis Ernesto; Romero, Graciela; Auteri, Carmelo; et al.; First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa); iMed Pub; Electronic Journal of Biology; 13; 1; 2-2017; 63-66
1860-3122
CONICET Digital
CONICET
url http://hdl.handle.net/11336/83370
identifier_str_mv Brihuega, Bibiana; Grune Loffler, Sylvia; Samartino, Luis Ernesto; Romero, Graciela; Auteri, Carmelo; et al.; First Isolation of Leptospira borgpetersenii from Fetuses of Wild Boars (Sus scrofa); iMed Pub; Electronic Journal of Biology; 13; 1; 2-2017; 63-66
1860-3122
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ejbio.imedpub.com/first-isolation-of-leptospira-borgpetersenii-from-fetuses-ofwild-boars-sus-scrofa.php?aid=18235
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv iMed Pub
publisher.none.fl_str_mv iMed Pub
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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