First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)

Autores
Brihuega, Bibiana Felicitas; Grune Loffler, Sylvia; Samartino, Luis Ernesto; Romero, Graciela Noemi; Auteri, Carmelo Domingo; Martinez, Mara Leila
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosis is maintained in nature by chronic renal infection of carrier animals, being rodents and other small mammals the most important reservoirs. Also, significant sources of human infection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa) is an introduced species from Europe which is widespread in America and particularly in the Center and South of Argentina. This species is highly valued by hunters in the region and their meat is consumed more often. In many countries of the world (Europe, USA and Australia) studies of seroprevalence have been carried out, the seroreactivity does not mean that the wild boar has clinical symptoms of leptospirosis or that wild boars are maintenance host of this pathogen. However, these animals have been in contact to leptospiras in their environment in the past. This study has the objective of molecular characterization of pathogenic Leptospira sp. isolated strain obtained from fetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wild boar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy was performed of the 4 fetuses and samples of kidneys, liver, spleen and lung were cultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung and kidney of fetuses were processed and analyzed by direct immunofluorescence. Multiple Locus Variable number tandems repeats Analysis (MLVA) was used to characterize the isolated strain. The genetic profile of the isolated pathogenic Leptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovar Castellonis Castellon 3. To the best of our knowledge this is the first isolation of Leptospira spp. from a wild boar fetus.
Instituto de Patobiología
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Auteri, Carmelo Domingo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fuente
Electronic journal of biology, 13 (1) : 63-66 (2017)
Materia
Enfermedades de los Animales
Sus Scrofa
Jabalí
Genotipos
Wild Boar
Genotypes
Leptospira borgpetersenii
Región Patagónica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/547
Acceder
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oai_identifier_str oai:localhost:20.500.12123/547
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)Brihuega, Bibiana FelicitasGrune Loffler, SylviaSamartino, Luis ErnestoRomero, Graciela NoemiAuteri, Carmelo DomingoMartinez, Mara LeilaEnfermedades de los AnimalesSus ScrofaJabalíGenotiposWild BoarGenotypesLeptospira borgpeterseniiRegión PatagónicaLeptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosis is maintained in nature by chronic renal infection of carrier animals, being rodents and other small mammals the most important reservoirs. Also, significant sources of human infection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa) is an introduced species from Europe which is widespread in America and particularly in the Center and South of Argentina. This species is highly valued by hunters in the region and their meat is consumed more often. In many countries of the world (Europe, USA and Australia) studies of seroprevalence have been carried out, the seroreactivity does not mean that the wild boar has clinical symptoms of leptospirosis or that wild boars are maintenance host of this pathogen. However, these animals have been in contact to leptospiras in their environment in the past. This study has the objective of molecular characterization of pathogenic Leptospira sp. isolated strain obtained from fetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wild boar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy was performed of the 4 fetuses and samples of kidneys, liver, spleen and lung were cultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung and kidney of fetuses were processed and analyzed by direct immunofluorescence. Multiple Locus Variable number tandems repeats Analysis (MLVA) was used to characterize the isolated strain. The genetic profile of the isolated pathogenic Leptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovar Castellonis Castellon 3. To the best of our knowledge this is the first isolation of Leptospira spp. from a wild boar fetus.Instituto de PatobiologíaFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Auteri, Carmelo Domingo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina2017-07-03T14:16:33Z2017-07-03T14:16:33Z2017-02-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/547http://ejbio.imedpub.com/first-isolation-of-leptospira-borgpetersenii-from-fetuses-ofwild-boars-sus-scrofa.pdf1860-3122Electronic journal of biology, 13 (1) : 63-66 (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengPatagonia (general region)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:28:56Zoai:localhost:20.500.12123/547instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:28:56.573INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)
title First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)
spellingShingle First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)
Brihuega, Bibiana Felicitas
Enfermedades de los Animales
Sus Scrofa
Jabalí
Genotipos
Wild Boar
Genotypes
Leptospira borgpetersenii
Región Patagónica
title_short First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)
title_full First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)
title_fullStr First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)
title_full_unstemmed First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)
title_sort First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)
dc.creator.none.fl_str_mv Brihuega, Bibiana Felicitas
Grune Loffler, Sylvia
Samartino, Luis Ernesto
Romero, Graciela Noemi
Auteri, Carmelo Domingo
Martinez, Mara Leila
author Brihuega, Bibiana Felicitas
author_facet Brihuega, Bibiana Felicitas
Grune Loffler, Sylvia
Samartino, Luis Ernesto
Romero, Graciela Noemi
Auteri, Carmelo Domingo
Martinez, Mara Leila
author_role author
author2 Grune Loffler, Sylvia
Samartino, Luis Ernesto
Romero, Graciela Noemi
Auteri, Carmelo Domingo
Martinez, Mara Leila
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Sus Scrofa
Jabalí
Genotipos
Wild Boar
Genotypes
Leptospira borgpetersenii
Región Patagónica
topic Enfermedades de los Animales
Sus Scrofa
Jabalí
Genotipos
Wild Boar
Genotypes
Leptospira borgpetersenii
Región Patagónica
dc.description.none.fl_txt_mv Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosis is maintained in nature by chronic renal infection of carrier animals, being rodents and other small mammals the most important reservoirs. Also, significant sources of human infection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa) is an introduced species from Europe which is widespread in America and particularly in the Center and South of Argentina. This species is highly valued by hunters in the region and their meat is consumed more often. In many countries of the world (Europe, USA and Australia) studies of seroprevalence have been carried out, the seroreactivity does not mean that the wild boar has clinical symptoms of leptospirosis or that wild boars are maintenance host of this pathogen. However, these animals have been in contact to leptospiras in their environment in the past. This study has the objective of molecular characterization of pathogenic Leptospira sp. isolated strain obtained from fetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wild boar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy was performed of the 4 fetuses and samples of kidneys, liver, spleen and lung were cultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung and kidney of fetuses were processed and analyzed by direct immunofluorescence. Multiple Locus Variable number tandems repeats Analysis (MLVA) was used to characterize the isolated strain. The genetic profile of the isolated pathogenic Leptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovar Castellonis Castellon 3. To the best of our knowledge this is the first isolation of Leptospira spp. from a wild boar fetus.
Instituto de Patobiología
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Auteri, Carmelo Domingo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
description Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosis is maintained in nature by chronic renal infection of carrier animals, being rodents and other small mammals the most important reservoirs. Also, significant sources of human infection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa) is an introduced species from Europe which is widespread in America and particularly in the Center and South of Argentina. This species is highly valued by hunters in the region and their meat is consumed more often. In many countries of the world (Europe, USA and Australia) studies of seroprevalence have been carried out, the seroreactivity does not mean that the wild boar has clinical symptoms of leptospirosis or that wild boars are maintenance host of this pathogen. However, these animals have been in contact to leptospiras in their environment in the past. This study has the objective of molecular characterization of pathogenic Leptospira sp. isolated strain obtained from fetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wild boar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy was performed of the 4 fetuses and samples of kidneys, liver, spleen and lung were cultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung and kidney of fetuses were processed and analyzed by direct immunofluorescence. Multiple Locus Variable number tandems repeats Analysis (MLVA) was used to characterize the isolated strain. The genetic profile of the isolated pathogenic Leptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovar Castellonis Castellon 3. To the best of our knowledge this is the first isolation of Leptospira spp. from a wild boar fetus.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-03T14:16:33Z
2017-07-03T14:16:33Z
2017-02-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/547
http://ejbio.imedpub.com/first-isolation-of-leptospira-borgpetersenii-from-fetuses-ofwild-boars-sus-scrofa.pdf
1860-3122
url http://hdl.handle.net/20.500.12123/547
http://ejbio.imedpub.com/first-isolation-of-leptospira-borgpetersenii-from-fetuses-ofwild-boars-sus-scrofa.pdf
identifier_str_mv 1860-3122
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Patagonia (general region)
dc.source.none.fl_str_mv Electronic journal of biology, 13 (1) : 63-66 (2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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