Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina

Autores
Soria, Mario; Godano, Eduardo Ignacio; Leiva, Leonardo Esteban; Shehata, Awad A.; Bueno, Dante Javier
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study aimed to evaluate the isolation methods of Salmonella from tissues and environmental samples collected during natural Salmonella outbreaks in commercial layer farms in Argentina. We also assessed key risk factors for contamination with Salmonella serovar Gallinarum biovar Gallinarum (SG) in poultry houses. To achieve this, we collected tissue samples from 20 houses (n=398; including spleen, liver, ovary, cecal contents, and bone marrow) and environmental samples (n=510; including feed, eggs, feces, and water) from deceased laying hens and their surroundings. Various isolation procedures were employed using different media. Salmonella was identified based on biochemical tests and serotyping using agglutination tests. Results showed that out of 398 tissue samples, 247 (62%) were positive for SG: bone marrow (62/80), spleen (59/79), liver (57/80), ovary (51/80), and cecum 18/79). Salmonella spp. was detected in 25% (1/4), 17% (2/12), 10.6% (10/94), and 3.6% (4/110) in boot swabs, egg nests, feces, and feed, respectively. Additionally, samples of yolk egg pool (1/74) and eggshell pool (1/74) were positive for SG. Among isolated serovars, S. ser. Cerro was the most frequently isolated serovar, followed by SG, S. ser. Livingstone, S. ser. Enteritidis, S. ser. Derby, S. ser. Corvallis, S. ser. Infantis, S. ser. Mbandaka, S. ser. Montevideo, S. ser. Schwarzengrund, and S. ser. Heidelberg, which were all isolated from environmental samples. Notably, four poultry houses exhibited contamination with multiple serotypes, with three or four serotypes present. Multiple logistic regression analyses revealed that vaccination against SG and effective insect control notably decreased the spread of Salmonella on poultry farms. Our results emphasize the importance of using diverse sample types and detection techniques for isolating and identifying Salmonella. This data could also contribute to developing improved control programs and intervention strategies at the farm level to minimize Salmonella contamination in poultry.
EEA Concepción del Uruguay
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; Argentina
Fil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; Argentina
Fil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; Argentina
Fil: Shehata, Awad A. Technical University of Munich (TUM). Bavarian NMR Center. Structural Membrane Biochemistry; Alemania
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos: Sede Basavilbaso. Facultad de Ciencia y Tecnología; Argentina
Fuente
German journal of veterinary research 5 (2): 1-14. (2025)
Materia
Salmonella
Aislamiento
Muestra de Tejido
Gallinas Ponedoras
Argentina
Isolation
Tissue Extracts
Layer Chickens
Muestras Ambientales
Granja de Gallinas Ponedoras
Environmental Samples
Laying-hen Farm
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/21818
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network_name_str INTA Digital (INTA)
spelling Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in ArgentinaSoria, MarioGodano, Eduardo IgnacioLeiva, Leonardo EstebanShehata, Awad A.Bueno, Dante JavierSalmonellaAislamientoMuestra de TejidoGallinas PonedorasArgentinaIsolationTissue ExtractsLayer ChickensMuestras AmbientalesGranja de Gallinas PonedorasEnvironmental SamplesLaying-hen FarmThis study aimed to evaluate the isolation methods of Salmonella from tissues and environmental samples collected during natural Salmonella outbreaks in commercial layer farms in Argentina. We also assessed key risk factors for contamination with Salmonella serovar Gallinarum biovar Gallinarum (SG) in poultry houses. To achieve this, we collected tissue samples from 20 houses (n=398; including spleen, liver, ovary, cecal contents, and bone marrow) and environmental samples (n=510; including feed, eggs, feces, and water) from deceased laying hens and their surroundings. Various isolation procedures were employed using different media. Salmonella was identified based on biochemical tests and serotyping using agglutination tests. Results showed that out of 398 tissue samples, 247 (62%) were positive for SG: bone marrow (62/80), spleen (59/79), liver (57/80), ovary (51/80), and cecum 18/79). Salmonella spp. was detected in 25% (1/4), 17% (2/12), 10.6% (10/94), and 3.6% (4/110) in boot swabs, egg nests, feces, and feed, respectively. Additionally, samples of yolk egg pool (1/74) and eggshell pool (1/74) were positive for SG. Among isolated serovars, S. ser. Cerro was the most frequently isolated serovar, followed by SG, S. ser. Livingstone, S. ser. Enteritidis, S. ser. Derby, S. ser. Corvallis, S. ser. Infantis, S. ser. Mbandaka, S. ser. Montevideo, S. ser. Schwarzengrund, and S. ser. Heidelberg, which were all isolated from environmental samples. Notably, four poultry houses exhibited contamination with multiple serotypes, with three or four serotypes present. Multiple logistic regression analyses revealed that vaccination against SG and effective insect control notably decreased the spread of Salmonella on poultry farms. Our results emphasize the importance of using diverse sample types and detection techniques for isolating and identifying Salmonella. This data could also contribute to developing improved control programs and intervention strategies at the farm level to minimize Salmonella contamination in poultry.EEA Concepción del UruguayFil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; ArgentinaFil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; ArgentinaFil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; ArgentinaFil: Shehata, Awad A. Technical University of Munich (TUM). Bavarian NMR Center. Structural Membrane Biochemistry; AlemaniaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos: Sede Basavilbaso. Facultad de Ciencia y Tecnología; ArgentinaGerman Multidisciplinary Publishing Center (GMPC)2025-03-26T11:40:08Z2025-03-26T11:40:08Z2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21818https://www.gmpc-akademie.de/articles/gjvr/single/248Soria, M. A., Godano, E. 1., Leiva, L. E., Shehata, A. A., and Bueno, D. J. 2025. Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina. Ger. J. Vet. Res. 5 (2): 1-14. https://doi.org/10.51585/gjvr.2025.2.01292703-1322https://doi.org/10.51585/gjvr.2025.2.0129German journal of veterinary research 5 (2): 1-14. (2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina .......... (nation) (World, South America)7006477info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:12Zoai:localhost:20.500.12123/21818instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:13.594INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina
title Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina
spellingShingle Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina
Soria, Mario
Salmonella
Aislamiento
Muestra de Tejido
Gallinas Ponedoras
Argentina
Isolation
Tissue Extracts
Layer Chickens
Muestras Ambientales
Granja de Gallinas Ponedoras
Environmental Samples
Laying-hen Farm
title_short Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina
title_full Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina
title_fullStr Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina
title_full_unstemmed Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina
title_sort Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina
dc.creator.none.fl_str_mv Soria, Mario
Godano, Eduardo Ignacio
Leiva, Leonardo Esteban
Shehata, Awad A.
Bueno, Dante Javier
author Soria, Mario
author_facet Soria, Mario
Godano, Eduardo Ignacio
Leiva, Leonardo Esteban
Shehata, Awad A.
Bueno, Dante Javier
author_role author
author2 Godano, Eduardo Ignacio
Leiva, Leonardo Esteban
Shehata, Awad A.
Bueno, Dante Javier
author2_role author
author
author
author
dc.subject.none.fl_str_mv Salmonella
Aislamiento
Muestra de Tejido
Gallinas Ponedoras
Argentina
Isolation
Tissue Extracts
Layer Chickens
Muestras Ambientales
Granja de Gallinas Ponedoras
Environmental Samples
Laying-hen Farm
topic Salmonella
Aislamiento
Muestra de Tejido
Gallinas Ponedoras
Argentina
Isolation
Tissue Extracts
Layer Chickens
Muestras Ambientales
Granja de Gallinas Ponedoras
Environmental Samples
Laying-hen Farm
dc.description.none.fl_txt_mv This study aimed to evaluate the isolation methods of Salmonella from tissues and environmental samples collected during natural Salmonella outbreaks in commercial layer farms in Argentina. We also assessed key risk factors for contamination with Salmonella serovar Gallinarum biovar Gallinarum (SG) in poultry houses. To achieve this, we collected tissue samples from 20 houses (n=398; including spleen, liver, ovary, cecal contents, and bone marrow) and environmental samples (n=510; including feed, eggs, feces, and water) from deceased laying hens and their surroundings. Various isolation procedures were employed using different media. Salmonella was identified based on biochemical tests and serotyping using agglutination tests. Results showed that out of 398 tissue samples, 247 (62%) were positive for SG: bone marrow (62/80), spleen (59/79), liver (57/80), ovary (51/80), and cecum 18/79). Salmonella spp. was detected in 25% (1/4), 17% (2/12), 10.6% (10/94), and 3.6% (4/110) in boot swabs, egg nests, feces, and feed, respectively. Additionally, samples of yolk egg pool (1/74) and eggshell pool (1/74) were positive for SG. Among isolated serovars, S. ser. Cerro was the most frequently isolated serovar, followed by SG, S. ser. Livingstone, S. ser. Enteritidis, S. ser. Derby, S. ser. Corvallis, S. ser. Infantis, S. ser. Mbandaka, S. ser. Montevideo, S. ser. Schwarzengrund, and S. ser. Heidelberg, which were all isolated from environmental samples. Notably, four poultry houses exhibited contamination with multiple serotypes, with three or four serotypes present. Multiple logistic regression analyses revealed that vaccination against SG and effective insect control notably decreased the spread of Salmonella on poultry farms. Our results emphasize the importance of using diverse sample types and detection techniques for isolating and identifying Salmonella. This data could also contribute to developing improved control programs and intervention strategies at the farm level to minimize Salmonella contamination in poultry.
EEA Concepción del Uruguay
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; Argentina
Fil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; Argentina
Fil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; Argentina
Fil: Shehata, Awad A. Technical University of Munich (TUM). Bavarian NMR Center. Structural Membrane Biochemistry; Alemania
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos: Sede Basavilbaso. Facultad de Ciencia y Tecnología; Argentina
description This study aimed to evaluate the isolation methods of Salmonella from tissues and environmental samples collected during natural Salmonella outbreaks in commercial layer farms in Argentina. We also assessed key risk factors for contamination with Salmonella serovar Gallinarum biovar Gallinarum (SG) in poultry houses. To achieve this, we collected tissue samples from 20 houses (n=398; including spleen, liver, ovary, cecal contents, and bone marrow) and environmental samples (n=510; including feed, eggs, feces, and water) from deceased laying hens and their surroundings. Various isolation procedures were employed using different media. Salmonella was identified based on biochemical tests and serotyping using agglutination tests. Results showed that out of 398 tissue samples, 247 (62%) were positive for SG: bone marrow (62/80), spleen (59/79), liver (57/80), ovary (51/80), and cecum 18/79). Salmonella spp. was detected in 25% (1/4), 17% (2/12), 10.6% (10/94), and 3.6% (4/110) in boot swabs, egg nests, feces, and feed, respectively. Additionally, samples of yolk egg pool (1/74) and eggshell pool (1/74) were positive for SG. Among isolated serovars, S. ser. Cerro was the most frequently isolated serovar, followed by SG, S. ser. Livingstone, S. ser. Enteritidis, S. ser. Derby, S. ser. Corvallis, S. ser. Infantis, S. ser. Mbandaka, S. ser. Montevideo, S. ser. Schwarzengrund, and S. ser. Heidelberg, which were all isolated from environmental samples. Notably, four poultry houses exhibited contamination with multiple serotypes, with three or four serotypes present. Multiple logistic regression analyses revealed that vaccination against SG and effective insect control notably decreased the spread of Salmonella on poultry farms. Our results emphasize the importance of using diverse sample types and detection techniques for isolating and identifying Salmonella. This data could also contribute to developing improved control programs and intervention strategies at the farm level to minimize Salmonella contamination in poultry.
publishDate 2025
dc.date.none.fl_str_mv 2025-03-26T11:40:08Z
2025-03-26T11:40:08Z
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21818
https://www.gmpc-akademie.de/articles/gjvr/single/248
Soria, M. A., Godano, E. 1., Leiva, L. E., Shehata, A. A., and Bueno, D. J. 2025. Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina. Ger. J. Vet. Res. 5 (2): 1-14. https://doi.org/10.51585/gjvr.2025.2.0129
2703-1322
https://doi.org/10.51585/gjvr.2025.2.0129
url http://hdl.handle.net/20.500.12123/21818
https://www.gmpc-akademie.de/articles/gjvr/single/248
https://doi.org/10.51585/gjvr.2025.2.0129
identifier_str_mv Soria, M. A., Godano, E. 1., Leiva, L. E., Shehata, A. A., and Bueno, D. J. 2025. Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina. Ger. J. Vet. Res. 5 (2): 1-14. https://doi.org/10.51585/gjvr.2025.2.0129
2703-1322
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina .......... (nation) (World, South America)
7006477
dc.publisher.none.fl_str_mv German Multidisciplinary Publishing Center (GMPC)
publisher.none.fl_str_mv German Multidisciplinary Publishing Center (GMPC)
dc.source.none.fl_str_mv German journal of veterinary research 5 (2): 1-14. (2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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