Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina
- Autores
- Soria, Mario; Godano, Eduardo Ignacio; Leiva, Leonardo Esteban; Shehata, Awad A.; Bueno, Dante Javier
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study aimed to evaluate the isolation methods of Salmonella from tissues and environmental samples collected during natural Salmonella outbreaks in commercial layer farms in Argentina. We also assessed key risk factors for contamination with Salmonella serovar Gallinarum biovar Gallinarum (SG) in poultry houses. To achieve this, we collected tissue samples from 20 houses (n=398; including spleen, liver, ovary, cecal contents, and bone marrow) and environmental samples (n=510; including feed, eggs, feces, and water) from deceased laying hens and their surroundings. Various isolation procedures were employed using different media. Salmonella was identified based on biochemical tests and serotyping using agglutination tests. Results showed that out of 398 tissue samples, 247 (62%) were positive for SG: bone marrow (62/80), spleen (59/79), liver (57/80), ovary (51/80), and cecum 18/79). Salmonella spp. was detected in 25% (1/4), 17% (2/12), 10.6% (10/94), and 3.6% (4/110) in boot swabs, egg nests, feces, and feed, respectively. Additionally, samples of yolk egg pool (1/74) and eggshell pool (1/74) were positive for SG. Among isolated serovars, S. ser. Cerro was the most frequently isolated serovar, followed by SG, S. ser. Livingstone, S. ser. Enteritidis, S. ser. Derby, S. ser. Corvallis, S. ser. Infantis, S. ser. Mbandaka, S. ser. Montevideo, S. ser. Schwarzengrund, and S. ser. Heidelberg, which were all isolated from environmental samples. Notably, four poultry houses exhibited contamination with multiple serotypes, with three or four serotypes present. Multiple logistic regression analyses revealed that vaccination against SG and effective insect control notably decreased the spread of Salmonella on poultry farms. Our results emphasize the importance of using diverse sample types and detection techniques for isolating and identifying Salmonella. This data could also contribute to developing improved control programs and intervention strategies at the farm level to minimize Salmonella contamination in poultry.
EEA Concepción del Uruguay
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; Argentina
Fil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; Argentina
Fil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; Argentina
Fil: Shehata, Awad A. Technical University of Munich (TUM). Bavarian NMR Center. Structural Membrane Biochemistry; Alemania
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos: Sede Basavilbaso. Facultad de Ciencia y Tecnología; Argentina - Fuente
- German journal of veterinary research 5 (2): 1-14. (2025)
- Materia
-
Salmonella
Aislamiento
Muestra de Tejido
Gallinas Ponedoras
Argentina
Isolation
Tissue Extracts
Layer Chickens
Muestras Ambientales
Granja de Gallinas Ponedoras
Environmental Samples
Laying-hen Farm - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/21818
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Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in ArgentinaSoria, MarioGodano, Eduardo IgnacioLeiva, Leonardo EstebanShehata, Awad A.Bueno, Dante JavierSalmonellaAislamientoMuestra de TejidoGallinas PonedorasArgentinaIsolationTissue ExtractsLayer ChickensMuestras AmbientalesGranja de Gallinas PonedorasEnvironmental SamplesLaying-hen FarmThis study aimed to evaluate the isolation methods of Salmonella from tissues and environmental samples collected during natural Salmonella outbreaks in commercial layer farms in Argentina. We also assessed key risk factors for contamination with Salmonella serovar Gallinarum biovar Gallinarum (SG) in poultry houses. To achieve this, we collected tissue samples from 20 houses (n=398; including spleen, liver, ovary, cecal contents, and bone marrow) and environmental samples (n=510; including feed, eggs, feces, and water) from deceased laying hens and their surroundings. Various isolation procedures were employed using different media. Salmonella was identified based on biochemical tests and serotyping using agglutination tests. Results showed that out of 398 tissue samples, 247 (62%) were positive for SG: bone marrow (62/80), spleen (59/79), liver (57/80), ovary (51/80), and cecum 18/79). Salmonella spp. was detected in 25% (1/4), 17% (2/12), 10.6% (10/94), and 3.6% (4/110) in boot swabs, egg nests, feces, and feed, respectively. Additionally, samples of yolk egg pool (1/74) and eggshell pool (1/74) were positive for SG. Among isolated serovars, S. ser. Cerro was the most frequently isolated serovar, followed by SG, S. ser. Livingstone, S. ser. Enteritidis, S. ser. Derby, S. ser. Corvallis, S. ser. Infantis, S. ser. Mbandaka, S. ser. Montevideo, S. ser. Schwarzengrund, and S. ser. Heidelberg, which were all isolated from environmental samples. Notably, four poultry houses exhibited contamination with multiple serotypes, with three or four serotypes present. Multiple logistic regression analyses revealed that vaccination against SG and effective insect control notably decreased the spread of Salmonella on poultry farms. Our results emphasize the importance of using diverse sample types and detection techniques for isolating and identifying Salmonella. This data could also contribute to developing improved control programs and intervention strategies at the farm level to minimize Salmonella contamination in poultry.EEA Concepción del UruguayFil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; ArgentinaFil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; ArgentinaFil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; ArgentinaFil: Shehata, Awad A. Technical University of Munich (TUM). Bavarian NMR Center. Structural Membrane Biochemistry; AlemaniaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos: Sede Basavilbaso. Facultad de Ciencia y Tecnología; ArgentinaGerman Multidisciplinary Publishing Center (GMPC)2025-03-26T11:40:08Z2025-03-26T11:40:08Z2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21818https://www.gmpc-akademie.de/articles/gjvr/single/248Soria, M. A., Godano, E. 1., Leiva, L. E., Shehata, A. A., and Bueno, D. J. 2025. Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina. Ger. J. Vet. Res. 5 (2): 1-14. https://doi.org/10.51585/gjvr.2025.2.01292703-1322https://doi.org/10.51585/gjvr.2025.2.0129German journal of veterinary research 5 (2): 1-14. (2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina .......... (nation) (World, South America)7006477info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:12Zoai:localhost:20.500.12123/21818instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:13.594INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina |
title |
Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina |
spellingShingle |
Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina Soria, Mario Salmonella Aislamiento Muestra de Tejido Gallinas Ponedoras Argentina Isolation Tissue Extracts Layer Chickens Muestras Ambientales Granja de Gallinas Ponedoras Environmental Samples Laying-hen Farm |
title_short |
Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina |
title_full |
Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina |
title_fullStr |
Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina |
title_full_unstemmed |
Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina |
title_sort |
Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina |
dc.creator.none.fl_str_mv |
Soria, Mario Godano, Eduardo Ignacio Leiva, Leonardo Esteban Shehata, Awad A. Bueno, Dante Javier |
author |
Soria, Mario |
author_facet |
Soria, Mario Godano, Eduardo Ignacio Leiva, Leonardo Esteban Shehata, Awad A. Bueno, Dante Javier |
author_role |
author |
author2 |
Godano, Eduardo Ignacio Leiva, Leonardo Esteban Shehata, Awad A. Bueno, Dante Javier |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Salmonella Aislamiento Muestra de Tejido Gallinas Ponedoras Argentina Isolation Tissue Extracts Layer Chickens Muestras Ambientales Granja de Gallinas Ponedoras Environmental Samples Laying-hen Farm |
topic |
Salmonella Aislamiento Muestra de Tejido Gallinas Ponedoras Argentina Isolation Tissue Extracts Layer Chickens Muestras Ambientales Granja de Gallinas Ponedoras Environmental Samples Laying-hen Farm |
dc.description.none.fl_txt_mv |
This study aimed to evaluate the isolation methods of Salmonella from tissues and environmental samples collected during natural Salmonella outbreaks in commercial layer farms in Argentina. We also assessed key risk factors for contamination with Salmonella serovar Gallinarum biovar Gallinarum (SG) in poultry houses. To achieve this, we collected tissue samples from 20 houses (n=398; including spleen, liver, ovary, cecal contents, and bone marrow) and environmental samples (n=510; including feed, eggs, feces, and water) from deceased laying hens and their surroundings. Various isolation procedures were employed using different media. Salmonella was identified based on biochemical tests and serotyping using agglutination tests. Results showed that out of 398 tissue samples, 247 (62%) were positive for SG: bone marrow (62/80), spleen (59/79), liver (57/80), ovary (51/80), and cecum 18/79). Salmonella spp. was detected in 25% (1/4), 17% (2/12), 10.6% (10/94), and 3.6% (4/110) in boot swabs, egg nests, feces, and feed, respectively. Additionally, samples of yolk egg pool (1/74) and eggshell pool (1/74) were positive for SG. Among isolated serovars, S. ser. Cerro was the most frequently isolated serovar, followed by SG, S. ser. Livingstone, S. ser. Enteritidis, S. ser. Derby, S. ser. Corvallis, S. ser. Infantis, S. ser. Mbandaka, S. ser. Montevideo, S. ser. Schwarzengrund, and S. ser. Heidelberg, which were all isolated from environmental samples. Notably, four poultry houses exhibited contamination with multiple serotypes, with three or four serotypes present. Multiple logistic regression analyses revealed that vaccination against SG and effective insect control notably decreased the spread of Salmonella on poultry farms. Our results emphasize the importance of using diverse sample types and detection techniques for isolating and identifying Salmonella. This data could also contribute to developing improved control programs and intervention strategies at the farm level to minimize Salmonella contamination in poultry. EEA Concepción del Uruguay Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; Argentina Fil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; Argentina Fil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; Argentina Fil: Shehata, Awad A. Technical University of Munich (TUM). Bavarian NMR Center. Structural Membrane Biochemistry; Alemania Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos: Sede Basavilbaso. Facultad de Ciencia y Tecnología; Argentina |
description |
This study aimed to evaluate the isolation methods of Salmonella from tissues and environmental samples collected during natural Salmonella outbreaks in commercial layer farms in Argentina. We also assessed key risk factors for contamination with Salmonella serovar Gallinarum biovar Gallinarum (SG) in poultry houses. To achieve this, we collected tissue samples from 20 houses (n=398; including spleen, liver, ovary, cecal contents, and bone marrow) and environmental samples (n=510; including feed, eggs, feces, and water) from deceased laying hens and their surroundings. Various isolation procedures were employed using different media. Salmonella was identified based on biochemical tests and serotyping using agglutination tests. Results showed that out of 398 tissue samples, 247 (62%) were positive for SG: bone marrow (62/80), spleen (59/79), liver (57/80), ovary (51/80), and cecum 18/79). Salmonella spp. was detected in 25% (1/4), 17% (2/12), 10.6% (10/94), and 3.6% (4/110) in boot swabs, egg nests, feces, and feed, respectively. Additionally, samples of yolk egg pool (1/74) and eggshell pool (1/74) were positive for SG. Among isolated serovars, S. ser. Cerro was the most frequently isolated serovar, followed by SG, S. ser. Livingstone, S. ser. Enteritidis, S. ser. Derby, S. ser. Corvallis, S. ser. Infantis, S. ser. Mbandaka, S. ser. Montevideo, S. ser. Schwarzengrund, and S. ser. Heidelberg, which were all isolated from environmental samples. Notably, four poultry houses exhibited contamination with multiple serotypes, with three or four serotypes present. Multiple logistic regression analyses revealed that vaccination against SG and effective insect control notably decreased the spread of Salmonella on poultry farms. Our results emphasize the importance of using diverse sample types and detection techniques for isolating and identifying Salmonella. This data could also contribute to developing improved control programs and intervention strategies at the farm level to minimize Salmonella contamination in poultry. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025-03-26T11:40:08Z 2025-03-26T11:40:08Z 2025 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/21818 https://www.gmpc-akademie.de/articles/gjvr/single/248 Soria, M. A., Godano, E. 1., Leiva, L. E., Shehata, A. A., and Bueno, D. J. 2025. Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina. Ger. J. Vet. Res. 5 (2): 1-14. https://doi.org/10.51585/gjvr.2025.2.0129 2703-1322 https://doi.org/10.51585/gjvr.2025.2.0129 |
url |
http://hdl.handle.net/20.500.12123/21818 https://www.gmpc-akademie.de/articles/gjvr/single/248 https://doi.org/10.51585/gjvr.2025.2.0129 |
identifier_str_mv |
Soria, M. A., Godano, E. 1., Leiva, L. E., Shehata, A. A., and Bueno, D. J. 2025. Isolation of Salmonella from tissue and environmental samples and assessment of risk factors in commercial layers in Argentina. Ger. J. Vet. Res. 5 (2): 1-14. https://doi.org/10.51585/gjvr.2025.2.0129 2703-1322 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Argentina .......... (nation) (World, South America) 7006477 |
dc.publisher.none.fl_str_mv |
German Multidisciplinary Publishing Center (GMPC) |
publisher.none.fl_str_mv |
German Multidisciplinary Publishing Center (GMPC) |
dc.source.none.fl_str_mv |
German journal of veterinary research 5 (2): 1-14. (2025) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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12.559606 |