Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats

Autores
Castro Pascual, Ivanna Carla; Pereira, Sorroche Bruna; Curzel, Florencia Elisa; Altamirano, Fernando Gabriel; Ferramola, Mariana Lucila; Lacoste, Gabriela; Delgado, Marcela; Martín, Mauricio Gerardo; Anzulovich, Ana Cecilia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The beneficial effects of CR would work through epigenetic processes, but this is largely unexplored. Here, we examine the hypothesis that CR recovers learning and motor coordination associated to changes in Bdnf and TrkB DNA methylation, in old rats. For that, male Holtzman rats -young (3-mo-old, n=8), old (22-mo-old, n=9), both fed ad-libitum, and 18-mo-old rats fed a 40% calorie restricted diet for 3 months (n=4)- were kept under 12h-light: 12h-dark, 22-24 °C and water ad-libitum. Performing the Single Reaching Pellet test, we observed that the percentage of the total success rate and the quality of the first attempt during performance are significantly lower in the old compared to the young and old rats with CR (p <0.05). As a control the olfactory sense and mood of the animals, the Buried Food Pellet and Corner Test were performed respectively. Samples (n=6) of cerebellar DNA from the three groups, were examined by bisulfite sequencing at CpG sites in regulatory regions in exons 3 and 9 of Bdnf and exon 1 of TrkB genes, to measure methylation with base-specificity. We observed 89 to 100% methylation at the CpG site in exon 9 of Bdnf gene, with no significant differences between groups. Methylations were not detected at the CpG sites in exon 3 of Bdnf nor exon 1 of TrkB gene, in any of the experimental groups. Our results show there is no association between cognitive CR benefits and CpG methylation of cerebellar Bdnf and TrkB genes in the studied exons.
Fil: Castro Pascual, Ivanna Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Pereira, Sorroche Bruna. Centro de Pesquisa em Oncologia Molecular; Brasil
Fil: Curzel, Florencia Elisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Altamirano, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Ferramola, Mariana Lucila. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Lacoste, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Delgado, Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Martín, Mauricio Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Anzulovich, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXV Annual Meeting of the Argentinian Society for Neuroscience Research
Buenos Aires
Argentina
Sociedad Argentina de Neurociencias
Materia
CALORIC RESTRICTION
AGING
CEREBELLUM
COGNITIVE ABILITIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181890
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181890
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Caloric restriction recovered cerebellar motor and cognitive abilities in aged ratsCastro Pascual, Ivanna CarlaPereira, Sorroche BrunaCurzel, Florencia ElisaAltamirano, Fernando GabrielFerramola, Mariana LucilaLacoste, GabrielaDelgado, MarcelaMartín, Mauricio GerardoAnzulovich, Ana CeciliaCALORIC RESTRICTIONAGINGCEREBELLUMCOGNITIVE ABILITIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The beneficial effects of CR would work through epigenetic processes, but this is largely unexplored. Here, we examine the hypothesis that CR recovers learning and motor coordination associated to changes in Bdnf and TrkB DNA methylation, in old rats. For that, male Holtzman rats -young (3-mo-old, n=8), old (22-mo-old, n=9), both fed ad-libitum, and 18-mo-old rats fed a 40% calorie restricted diet for 3 months (n=4)- were kept under 12h-light: 12h-dark, 22-24 °C and water ad-libitum. Performing the Single Reaching Pellet test, we observed that the percentage of the total success rate and the quality of the first attempt during performance are significantly lower in the old compared to the young and old rats with CR (p <0.05). As a control the olfactory sense and mood of the animals, the Buried Food Pellet and Corner Test were performed respectively. Samples (n=6) of cerebellar DNA from the three groups, were examined by bisulfite sequencing at CpG sites in regulatory regions in exons 3 and 9 of Bdnf and exon 1 of TrkB genes, to measure methylation with base-specificity. We observed 89 to 100% methylation at the CpG site in exon 9 of Bdnf gene, with no significant differences between groups. Methylations were not detected at the CpG sites in exon 3 of Bdnf nor exon 1 of TrkB gene, in any of the experimental groups. Our results show there is no association between cognitive CR benefits and CpG methylation of cerebellar Bdnf and TrkB genes in the studied exons.Fil: Castro Pascual, Ivanna Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Pereira, Sorroche Bruna. Centro de Pesquisa em Oncologia Molecular; BrasilFil: Curzel, Florencia Elisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Altamirano, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Ferramola, Mariana Lucila. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Lacoste, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Delgado, Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Martín, Mauricio Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Anzulovich, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaXXV Annual Meeting of the Argentinian Society for Neuroscience ResearchBuenos AiresArgentinaSociedad Argentina de NeurocienciasSociedad Argentina de Neurociencias2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/181890Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats; XXV Annual Meeting of the Argentinian Society for Neuroscience Research; Buenos Aires; Argentina; 2020; 167-267CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://san2020.saneurociencias.org.ar/wp-content/uploads/2021/06/EBOOK-2020.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:05Zoai:ri.conicet.gov.ar:11336/181890instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:05.899CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats
title Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats
spellingShingle Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats
Castro Pascual, Ivanna Carla
CALORIC RESTRICTION
AGING
CEREBELLUM
COGNITIVE ABILITIES
title_short Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats
title_full Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats
title_fullStr Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats
title_full_unstemmed Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats
title_sort Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats
dc.creator.none.fl_str_mv Castro Pascual, Ivanna Carla
Pereira, Sorroche Bruna
Curzel, Florencia Elisa
Altamirano, Fernando Gabriel
Ferramola, Mariana Lucila
Lacoste, Gabriela
Delgado, Marcela
Martín, Mauricio Gerardo
Anzulovich, Ana Cecilia
author Castro Pascual, Ivanna Carla
author_facet Castro Pascual, Ivanna Carla
Pereira, Sorroche Bruna
Curzel, Florencia Elisa
Altamirano, Fernando Gabriel
Ferramola, Mariana Lucila
Lacoste, Gabriela
Delgado, Marcela
Martín, Mauricio Gerardo
Anzulovich, Ana Cecilia
author_role author
author2 Pereira, Sorroche Bruna
Curzel, Florencia Elisa
Altamirano, Fernando Gabriel
Ferramola, Mariana Lucila
Lacoste, Gabriela
Delgado, Marcela
Martín, Mauricio Gerardo
Anzulovich, Ana Cecilia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CALORIC RESTRICTION
AGING
CEREBELLUM
COGNITIVE ABILITIES
topic CALORIC RESTRICTION
AGING
CEREBELLUM
COGNITIVE ABILITIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The beneficial effects of CR would work through epigenetic processes, but this is largely unexplored. Here, we examine the hypothesis that CR recovers learning and motor coordination associated to changes in Bdnf and TrkB DNA methylation, in old rats. For that, male Holtzman rats -young (3-mo-old, n=8), old (22-mo-old, n=9), both fed ad-libitum, and 18-mo-old rats fed a 40% calorie restricted diet for 3 months (n=4)- were kept under 12h-light: 12h-dark, 22-24 °C and water ad-libitum. Performing the Single Reaching Pellet test, we observed that the percentage of the total success rate and the quality of the first attempt during performance are significantly lower in the old compared to the young and old rats with CR (p <0.05). As a control the olfactory sense and mood of the animals, the Buried Food Pellet and Corner Test were performed respectively. Samples (n=6) of cerebellar DNA from the three groups, were examined by bisulfite sequencing at CpG sites in regulatory regions in exons 3 and 9 of Bdnf and exon 1 of TrkB genes, to measure methylation with base-specificity. We observed 89 to 100% methylation at the CpG site in exon 9 of Bdnf gene, with no significant differences between groups. Methylations were not detected at the CpG sites in exon 3 of Bdnf nor exon 1 of TrkB gene, in any of the experimental groups. Our results show there is no association between cognitive CR benefits and CpG methylation of cerebellar Bdnf and TrkB genes in the studied exons.
Fil: Castro Pascual, Ivanna Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Pereira, Sorroche Bruna. Centro de Pesquisa em Oncologia Molecular; Brasil
Fil: Curzel, Florencia Elisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Altamirano, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Ferramola, Mariana Lucila. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Lacoste, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Delgado, Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Martín, Mauricio Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Anzulovich, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXV Annual Meeting of the Argentinian Society for Neuroscience Research
Buenos Aires
Argentina
Sociedad Argentina de Neurociencias
description The beneficial effects of CR would work through epigenetic processes, but this is largely unexplored. Here, we examine the hypothesis that CR recovers learning and motor coordination associated to changes in Bdnf and TrkB DNA methylation, in old rats. For that, male Holtzman rats -young (3-mo-old, n=8), old (22-mo-old, n=9), both fed ad-libitum, and 18-mo-old rats fed a 40% calorie restricted diet for 3 months (n=4)- were kept under 12h-light: 12h-dark, 22-24 °C and water ad-libitum. Performing the Single Reaching Pellet test, we observed that the percentage of the total success rate and the quality of the first attempt during performance are significantly lower in the old compared to the young and old rats with CR (p <0.05). As a control the olfactory sense and mood of the animals, the Buried Food Pellet and Corner Test were performed respectively. Samples (n=6) of cerebellar DNA from the three groups, were examined by bisulfite sequencing at CpG sites in regulatory regions in exons 3 and 9 of Bdnf and exon 1 of TrkB genes, to measure methylation with base-specificity. We observed 89 to 100% methylation at the CpG site in exon 9 of Bdnf gene, with no significant differences between groups. Methylations were not detected at the CpG sites in exon 3 of Bdnf nor exon 1 of TrkB gene, in any of the experimental groups. Our results show there is no association between cognitive CR benefits and CpG methylation of cerebellar Bdnf and TrkB genes in the studied exons.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181890
Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats; XXV Annual Meeting of the Argentinian Society for Neuroscience Research; Buenos Aires; Argentina; 2020; 167-267
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181890
identifier_str_mv Caloric restriction recovered cerebellar motor and cognitive abilities in aged rats; XXV Annual Meeting of the Argentinian Society for Neuroscience Research; Buenos Aires; Argentina; 2020; 167-267
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://san2020.saneurociencias.org.ar/wp-content/uploads/2021/06/EBOOK-2020.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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