Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation

Autores
Konieczna, Jadwiga; García, Ana Paula; Sanchez, Juana Elisabet; Palou, Mariona; Palou, Andreu; Picó, Catalina
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A poor prenatal environment brings about perturbations in leptin surge and hypothalamic circuitry that program impaired ability to regulate energy homeostasis in adulthood. Here, using a rat model of moderate maternal caloric restriction during gestation, we aimed to investigate whether leptin supplementation with physiological doses throughout lactation is able to ameliorate the adverse developmental malprogramming effects exerted in offspring hypothalamus structure and function. Three groups of male and female rats were studied: the offspring of ad libitum fed dams (controls), the offspring of 20% calorie restricted dams during the first part of pregnancy (CR), and CR rats supplemented with physiological doses of leptin throughout lactation (CR-Leptin). Animals were sacrificed on postnatal day 25. Morphometric and immunohistochemical studies on arcuate (ARC) and paraventicular (PVN) nucleus were performed and hypothalamic expression levels of selected genes were determined. In CR males, leptin treatment restored, at least in part, the number of immunoreactive neuropeptide Y (NPY+) cells in ARC, the total number of cells in PVN, hypothalamic NPY, cocaine- and amphetamine-regulated transcript (CART) and suppressor of cytokine signalling-3 (SOCS-3) mRNA levels, and plasma leptin levels, which were decreased in CR animals. CR-Leptin males showed higher hypothalamic long-form leptin receptor (ObRb) mRNA levels, compared to control and CR animals. In CR females, leptin treatment reverted the increased number of cells in ARC and cell density in ARC and PVN, and reduced hypothalamic SOCS-3 mRNA expression to levels similar to controls. Leptin treatment also reverted the increased relative area of NPY+ fibers in the PVN occurring in CR animals. In conclusion, leptin supplementation throughout lactation is able to revert developmental effects on hypothalamic structure and function, making the metabolic malprogramming induced by maternal caloric restriction during gestation reversible to some extent.
Fil: Konieczna, Jadwiga. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: García, Ana Paula. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Juana Elisabet. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: Palou, Mariona. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: Palou, Andreu. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: Picó, Catalina. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Materia
CALORIC RESTRICTION
HYPOTHALAMUS
LEPTIN
METHABOLIC PROGRAMMING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/108734
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during GestationKonieczna, JadwigaGarcía, Ana PaulaSanchez, Juana ElisabetPalou, MarionaPalou, AndreuPicó, CatalinaCALORIC RESTRICTIONHYPOTHALAMUSLEPTINMETHABOLIC PROGRAMMINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A poor prenatal environment brings about perturbations in leptin surge and hypothalamic circuitry that program impaired ability to regulate energy homeostasis in adulthood. Here, using a rat model of moderate maternal caloric restriction during gestation, we aimed to investigate whether leptin supplementation with physiological doses throughout lactation is able to ameliorate the adverse developmental malprogramming effects exerted in offspring hypothalamus structure and function. Three groups of male and female rats were studied: the offspring of ad libitum fed dams (controls), the offspring of 20% calorie restricted dams during the first part of pregnancy (CR), and CR rats supplemented with physiological doses of leptin throughout lactation (CR-Leptin). Animals were sacrificed on postnatal day 25. Morphometric and immunohistochemical studies on arcuate (ARC) and paraventicular (PVN) nucleus were performed and hypothalamic expression levels of selected genes were determined. In CR males, leptin treatment restored, at least in part, the number of immunoreactive neuropeptide Y (NPY+) cells in ARC, the total number of cells in PVN, hypothalamic NPY, cocaine- and amphetamine-regulated transcript (CART) and suppressor of cytokine signalling-3 (SOCS-3) mRNA levels, and plasma leptin levels, which were decreased in CR animals. CR-Leptin males showed higher hypothalamic long-form leptin receptor (ObRb) mRNA levels, compared to control and CR animals. In CR females, leptin treatment reverted the increased number of cells in ARC and cell density in ARC and PVN, and reduced hypothalamic SOCS-3 mRNA expression to levels similar to controls. Leptin treatment also reverted the increased relative area of NPY+ fibers in the PVN occurring in CR animals. In conclusion, leptin supplementation throughout lactation is able to revert developmental effects on hypothalamic structure and function, making the metabolic malprogramming induced by maternal caloric restriction during gestation reversible to some extent.Fil: Konieczna, Jadwiga. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaFil: García, Ana Paula. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanchez, Juana Elisabet. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaFil: Palou, Mariona. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaFil: Palou, Andreu. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaFil: Picó, Catalina. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaPublic Library of Science2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/108734Konieczna, Jadwiga; García, Ana Paula; Sanchez, Juana Elisabet; Palou, Mariona; Palou, Andreu; et al.; Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation; Public Library of Science; Plos One; 8; 11; 11-2013; 1-141932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081906info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842976/info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0081906info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:29Zoai:ri.conicet.gov.ar:11336/108734instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:29.408CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation
title Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation
spellingShingle Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation
Konieczna, Jadwiga
CALORIC RESTRICTION
HYPOTHALAMUS
LEPTIN
METHABOLIC PROGRAMMING
title_short Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation
title_full Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation
title_fullStr Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation
title_full_unstemmed Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation
title_sort Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation
dc.creator.none.fl_str_mv Konieczna, Jadwiga
García, Ana Paula
Sanchez, Juana Elisabet
Palou, Mariona
Palou, Andreu
Picó, Catalina
author Konieczna, Jadwiga
author_facet Konieczna, Jadwiga
García, Ana Paula
Sanchez, Juana Elisabet
Palou, Mariona
Palou, Andreu
Picó, Catalina
author_role author
author2 García, Ana Paula
Sanchez, Juana Elisabet
Palou, Mariona
Palou, Andreu
Picó, Catalina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CALORIC RESTRICTION
HYPOTHALAMUS
LEPTIN
METHABOLIC PROGRAMMING
topic CALORIC RESTRICTION
HYPOTHALAMUS
LEPTIN
METHABOLIC PROGRAMMING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A poor prenatal environment brings about perturbations in leptin surge and hypothalamic circuitry that program impaired ability to regulate energy homeostasis in adulthood. Here, using a rat model of moderate maternal caloric restriction during gestation, we aimed to investigate whether leptin supplementation with physiological doses throughout lactation is able to ameliorate the adverse developmental malprogramming effects exerted in offspring hypothalamus structure and function. Three groups of male and female rats were studied: the offspring of ad libitum fed dams (controls), the offspring of 20% calorie restricted dams during the first part of pregnancy (CR), and CR rats supplemented with physiological doses of leptin throughout lactation (CR-Leptin). Animals were sacrificed on postnatal day 25. Morphometric and immunohistochemical studies on arcuate (ARC) and paraventicular (PVN) nucleus were performed and hypothalamic expression levels of selected genes were determined. In CR males, leptin treatment restored, at least in part, the number of immunoreactive neuropeptide Y (NPY+) cells in ARC, the total number of cells in PVN, hypothalamic NPY, cocaine- and amphetamine-regulated transcript (CART) and suppressor of cytokine signalling-3 (SOCS-3) mRNA levels, and plasma leptin levels, which were decreased in CR animals. CR-Leptin males showed higher hypothalamic long-form leptin receptor (ObRb) mRNA levels, compared to control and CR animals. In CR females, leptin treatment reverted the increased number of cells in ARC and cell density in ARC and PVN, and reduced hypothalamic SOCS-3 mRNA expression to levels similar to controls. Leptin treatment also reverted the increased relative area of NPY+ fibers in the PVN occurring in CR animals. In conclusion, leptin supplementation throughout lactation is able to revert developmental effects on hypothalamic structure and function, making the metabolic malprogramming induced by maternal caloric restriction during gestation reversible to some extent.
Fil: Konieczna, Jadwiga. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: García, Ana Paula. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Juana Elisabet. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: Palou, Mariona. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: Palou, Andreu. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: Picó, Catalina. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
description A poor prenatal environment brings about perturbations in leptin surge and hypothalamic circuitry that program impaired ability to regulate energy homeostasis in adulthood. Here, using a rat model of moderate maternal caloric restriction during gestation, we aimed to investigate whether leptin supplementation with physiological doses throughout lactation is able to ameliorate the adverse developmental malprogramming effects exerted in offspring hypothalamus structure and function. Three groups of male and female rats were studied: the offspring of ad libitum fed dams (controls), the offspring of 20% calorie restricted dams during the first part of pregnancy (CR), and CR rats supplemented with physiological doses of leptin throughout lactation (CR-Leptin). Animals were sacrificed on postnatal day 25. Morphometric and immunohistochemical studies on arcuate (ARC) and paraventicular (PVN) nucleus were performed and hypothalamic expression levels of selected genes were determined. In CR males, leptin treatment restored, at least in part, the number of immunoreactive neuropeptide Y (NPY+) cells in ARC, the total number of cells in PVN, hypothalamic NPY, cocaine- and amphetamine-regulated transcript (CART) and suppressor of cytokine signalling-3 (SOCS-3) mRNA levels, and plasma leptin levels, which were decreased in CR animals. CR-Leptin males showed higher hypothalamic long-form leptin receptor (ObRb) mRNA levels, compared to control and CR animals. In CR females, leptin treatment reverted the increased number of cells in ARC and cell density in ARC and PVN, and reduced hypothalamic SOCS-3 mRNA expression to levels similar to controls. Leptin treatment also reverted the increased relative area of NPY+ fibers in the PVN occurring in CR animals. In conclusion, leptin supplementation throughout lactation is able to revert developmental effects on hypothalamic structure and function, making the metabolic malprogramming induced by maternal caloric restriction during gestation reversible to some extent.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/108734
Konieczna, Jadwiga; García, Ana Paula; Sanchez, Juana Elisabet; Palou, Mariona; Palou, Andreu; et al.; Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation; Public Library of Science; Plos One; 8; 11; 11-2013; 1-14
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/108734
identifier_str_mv Konieczna, Jadwiga; García, Ana Paula; Sanchez, Juana Elisabet; Palou, Mariona; Palou, Andreu; et al.; Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation; Public Library of Science; Plos One; 8; 11; 11-2013; 1-14
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081906
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842976/
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0081906
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/zip
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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