Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation
- Autores
- Konieczna, Jadwiga; García, Ana Paula; Sanchez, Juana Elisabet; Palou, Mariona; Palou, Andreu; Picó, Catalina
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A poor prenatal environment brings about perturbations in leptin surge and hypothalamic circuitry that program impaired ability to regulate energy homeostasis in adulthood. Here, using a rat model of moderate maternal caloric restriction during gestation, we aimed to investigate whether leptin supplementation with physiological doses throughout lactation is able to ameliorate the adverse developmental malprogramming effects exerted in offspring hypothalamus structure and function. Three groups of male and female rats were studied: the offspring of ad libitum fed dams (controls), the offspring of 20% calorie restricted dams during the first part of pregnancy (CR), and CR rats supplemented with physiological doses of leptin throughout lactation (CR-Leptin). Animals were sacrificed on postnatal day 25. Morphometric and immunohistochemical studies on arcuate (ARC) and paraventicular (PVN) nucleus were performed and hypothalamic expression levels of selected genes were determined. In CR males, leptin treatment restored, at least in part, the number of immunoreactive neuropeptide Y (NPY+) cells in ARC, the total number of cells in PVN, hypothalamic NPY, cocaine- and amphetamine-regulated transcript (CART) and suppressor of cytokine signalling-3 (SOCS-3) mRNA levels, and plasma leptin levels, which were decreased in CR animals. CR-Leptin males showed higher hypothalamic long-form leptin receptor (ObRb) mRNA levels, compared to control and CR animals. In CR females, leptin treatment reverted the increased number of cells in ARC and cell density in ARC and PVN, and reduced hypothalamic SOCS-3 mRNA expression to levels similar to controls. Leptin treatment also reverted the increased relative area of NPY+ fibers in the PVN occurring in CR animals. In conclusion, leptin supplementation throughout lactation is able to revert developmental effects on hypothalamic structure and function, making the metabolic malprogramming induced by maternal caloric restriction during gestation reversible to some extent.
Fil: Konieczna, Jadwiga. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: García, Ana Paula. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Juana Elisabet. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: Palou, Mariona. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: Palou, Andreu. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España
Fil: Picó, Catalina. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España - Materia
-
CALORIC RESTRICTION
HYPOTHALAMUS
LEPTIN
METHABOLIC PROGRAMMING - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/108734
Ver los metadatos del registro completo
id |
CONICETDig_5eb35e193f65d96e694b39dfce12f507 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/108734 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during GestationKonieczna, JadwigaGarcía, Ana PaulaSanchez, Juana ElisabetPalou, MarionaPalou, AndreuPicó, CatalinaCALORIC RESTRICTIONHYPOTHALAMUSLEPTINMETHABOLIC PROGRAMMINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A poor prenatal environment brings about perturbations in leptin surge and hypothalamic circuitry that program impaired ability to regulate energy homeostasis in adulthood. Here, using a rat model of moderate maternal caloric restriction during gestation, we aimed to investigate whether leptin supplementation with physiological doses throughout lactation is able to ameliorate the adverse developmental malprogramming effects exerted in offspring hypothalamus structure and function. Three groups of male and female rats were studied: the offspring of ad libitum fed dams (controls), the offspring of 20% calorie restricted dams during the first part of pregnancy (CR), and CR rats supplemented with physiological doses of leptin throughout lactation (CR-Leptin). Animals were sacrificed on postnatal day 25. Morphometric and immunohistochemical studies on arcuate (ARC) and paraventicular (PVN) nucleus were performed and hypothalamic expression levels of selected genes were determined. In CR males, leptin treatment restored, at least in part, the number of immunoreactive neuropeptide Y (NPY+) cells in ARC, the total number of cells in PVN, hypothalamic NPY, cocaine- and amphetamine-regulated transcript (CART) and suppressor of cytokine signalling-3 (SOCS-3) mRNA levels, and plasma leptin levels, which were decreased in CR animals. CR-Leptin males showed higher hypothalamic long-form leptin receptor (ObRb) mRNA levels, compared to control and CR animals. In CR females, leptin treatment reverted the increased number of cells in ARC and cell density in ARC and PVN, and reduced hypothalamic SOCS-3 mRNA expression to levels similar to controls. Leptin treatment also reverted the increased relative area of NPY+ fibers in the PVN occurring in CR animals. In conclusion, leptin supplementation throughout lactation is able to revert developmental effects on hypothalamic structure and function, making the metabolic malprogramming induced by maternal caloric restriction during gestation reversible to some extent.Fil: Konieczna, Jadwiga. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaFil: García, Ana Paula. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanchez, Juana Elisabet. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaFil: Palou, Mariona. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaFil: Palou, Andreu. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaFil: Picó, Catalina. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; EspañaPublic Library of Science2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/108734Konieczna, Jadwiga; García, Ana Paula; Sanchez, Juana Elisabet; Palou, Mariona; Palou, Andreu; et al.; Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation; Public Library of Science; Plos One; 8; 11; 11-2013; 1-141932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081906info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842976/info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0081906info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:00Zoai:ri.conicet.gov.ar:11336/108734instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:00.9CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation |
title |
Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation |
spellingShingle |
Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation Konieczna, Jadwiga CALORIC RESTRICTION HYPOTHALAMUS LEPTIN METHABOLIC PROGRAMMING |
title_short |
Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation |
title_full |
Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation |
title_fullStr |
Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation |
title_full_unstemmed |
Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation |
title_sort |
Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation |
dc.creator.none.fl_str_mv |
Konieczna, Jadwiga García, Ana Paula Sanchez, Juana Elisabet Palou, Mariona Palou, Andreu Picó, Catalina |
author |
Konieczna, Jadwiga |
author_facet |
Konieczna, Jadwiga García, Ana Paula Sanchez, Juana Elisabet Palou, Mariona Palou, Andreu Picó, Catalina |
author_role |
author |
author2 |
García, Ana Paula Sanchez, Juana Elisabet Palou, Mariona Palou, Andreu Picó, Catalina |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CALORIC RESTRICTION HYPOTHALAMUS LEPTIN METHABOLIC PROGRAMMING |
topic |
CALORIC RESTRICTION HYPOTHALAMUS LEPTIN METHABOLIC PROGRAMMING |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A poor prenatal environment brings about perturbations in leptin surge and hypothalamic circuitry that program impaired ability to regulate energy homeostasis in adulthood. Here, using a rat model of moderate maternal caloric restriction during gestation, we aimed to investigate whether leptin supplementation with physiological doses throughout lactation is able to ameliorate the adverse developmental malprogramming effects exerted in offspring hypothalamus structure and function. Three groups of male and female rats were studied: the offspring of ad libitum fed dams (controls), the offspring of 20% calorie restricted dams during the first part of pregnancy (CR), and CR rats supplemented with physiological doses of leptin throughout lactation (CR-Leptin). Animals were sacrificed on postnatal day 25. Morphometric and immunohistochemical studies on arcuate (ARC) and paraventicular (PVN) nucleus were performed and hypothalamic expression levels of selected genes were determined. In CR males, leptin treatment restored, at least in part, the number of immunoreactive neuropeptide Y (NPY+) cells in ARC, the total number of cells in PVN, hypothalamic NPY, cocaine- and amphetamine-regulated transcript (CART) and suppressor of cytokine signalling-3 (SOCS-3) mRNA levels, and plasma leptin levels, which were decreased in CR animals. CR-Leptin males showed higher hypothalamic long-form leptin receptor (ObRb) mRNA levels, compared to control and CR animals. In CR females, leptin treatment reverted the increased number of cells in ARC and cell density in ARC and PVN, and reduced hypothalamic SOCS-3 mRNA expression to levels similar to controls. Leptin treatment also reverted the increased relative area of NPY+ fibers in the PVN occurring in CR animals. In conclusion, leptin supplementation throughout lactation is able to revert developmental effects on hypothalamic structure and function, making the metabolic malprogramming induced by maternal caloric restriction during gestation reversible to some extent. Fil: Konieczna, Jadwiga. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España Fil: García, Ana Paula. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sanchez, Juana Elisabet. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España Fil: Palou, Mariona. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España Fil: Palou, Andreu. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España Fil: Picó, Catalina. Universidad de Las Islas Baleares. Departamento de Biología Fundamental y Cs. de la Salud. Laboratorio de Biología Molecular, Nutrición y Biotecnología; España |
description |
A poor prenatal environment brings about perturbations in leptin surge and hypothalamic circuitry that program impaired ability to regulate energy homeostasis in adulthood. Here, using a rat model of moderate maternal caloric restriction during gestation, we aimed to investigate whether leptin supplementation with physiological doses throughout lactation is able to ameliorate the adverse developmental malprogramming effects exerted in offspring hypothalamus structure and function. Three groups of male and female rats were studied: the offspring of ad libitum fed dams (controls), the offspring of 20% calorie restricted dams during the first part of pregnancy (CR), and CR rats supplemented with physiological doses of leptin throughout lactation (CR-Leptin). Animals were sacrificed on postnatal day 25. Morphometric and immunohistochemical studies on arcuate (ARC) and paraventicular (PVN) nucleus were performed and hypothalamic expression levels of selected genes were determined. In CR males, leptin treatment restored, at least in part, the number of immunoreactive neuropeptide Y (NPY+) cells in ARC, the total number of cells in PVN, hypothalamic NPY, cocaine- and amphetamine-regulated transcript (CART) and suppressor of cytokine signalling-3 (SOCS-3) mRNA levels, and plasma leptin levels, which were decreased in CR animals. CR-Leptin males showed higher hypothalamic long-form leptin receptor (ObRb) mRNA levels, compared to control and CR animals. In CR females, leptin treatment reverted the increased number of cells in ARC and cell density in ARC and PVN, and reduced hypothalamic SOCS-3 mRNA expression to levels similar to controls. Leptin treatment also reverted the increased relative area of NPY+ fibers in the PVN occurring in CR animals. In conclusion, leptin supplementation throughout lactation is able to revert developmental effects on hypothalamic structure and function, making the metabolic malprogramming induced by maternal caloric restriction during gestation reversible to some extent. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/108734 Konieczna, Jadwiga; García, Ana Paula; Sanchez, Juana Elisabet; Palou, Mariona; Palou, Andreu; et al.; Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation; Public Library of Science; Plos One; 8; 11; 11-2013; 1-14 1932-6203 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/108734 |
identifier_str_mv |
Konieczna, Jadwiga; García, Ana Paula; Sanchez, Juana Elisabet; Palou, Mariona; Palou, Andreu; et al.; Oral Leptin Treatment in Suckling Rats Ameliorates Detrimental Effects in Hypothalamic Structure and Function Caused by Maternal Caloric Restriction during Gestation; Public Library of Science; Plos One; 8; 11; 11-2013; 1-14 1932-6203 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081906 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842976/ info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0081906 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/zip application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842270027381735424 |
score |
13.13397 |