Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles

Autores
Ohtake, Satoshi; Schebor, Carolina Claudia; de Pablo, Juan J.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC–cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDSC and DSC are used to examine the phase behavior of hydrated and dehydrated vesicles, respectively. The concentration of trehalose inside and outside the vesicles is manipulated, and is shown to affect the relative stability of the membranes. Our results show for the first time that a combination of high inner and low outer trehalose concentration is able to decrease the gel-to-liquid crystalline phase temperature (Tm), while any other combination will not. Upon dehydration, the Tm of all lipid mixtures increases. The extent of the increase depends on the trehalose distribution across the bilayer. The Tm changes in the same direction with trehalose concentration for both freeze-dried and fully hydrated samples, suggesting that the trehalose distribution across the vesicle membrane, as well as the trehalose–phospholipid interaction, is maintained upon lyophilization. The results presented in this work may aid in the formulation of systems to be used in the lyophilization of liposomes for drug delivery applications.
Fil: Ohtake, Satoshi. University of Wisconsin; Estados Unidos
Fil: Schebor, Carolina Claudia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Pablo, Juan J.. University of Wisconsin; Estados Unidos
Materia
Trehalose
Membanes
Cholesterol
Dppc
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16623
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16623
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesiclesOhtake, SatoshiSchebor, Carolina Claudiade Pablo, Juan J.TrehaloseMembanesCholesterolDppchttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC–cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDSC and DSC are used to examine the phase behavior of hydrated and dehydrated vesicles, respectively. The concentration of trehalose inside and outside the vesicles is manipulated, and is shown to affect the relative stability of the membranes. Our results show for the first time that a combination of high inner and low outer trehalose concentration is able to decrease the gel-to-liquid crystalline phase temperature (Tm), while any other combination will not. Upon dehydration, the Tm of all lipid mixtures increases. The extent of the increase depends on the trehalose distribution across the bilayer. The Tm changes in the same direction with trehalose concentration for both freeze-dried and fully hydrated samples, suggesting that the trehalose distribution across the vesicle membrane, as well as the trehalose–phospholipid interaction, is maintained upon lyophilization. The results presented in this work may aid in the formulation of systems to be used in the lyophilization of liposomes for drug delivery applications.Fil: Ohtake, Satoshi. University of Wisconsin; Estados UnidosFil: Schebor, Carolina Claudia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de Pablo, Juan J.. University of Wisconsin; Estados UnidosElsevier Science2006-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16623Ohtake, Satoshi; Schebor, Carolina Claudia; de Pablo, Juan J.; Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles; Elsevier Science; Biochimica Et Biophysica Acta - Biomembranes; 1758; 1; 1-2006; 65-730005-2736enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2006.01.002info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0005273606000071info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:40:26Zoai:ri.conicet.gov.ar:11336/16623instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:40:26.633CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles
title Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles
spellingShingle Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles
Ohtake, Satoshi
Trehalose
Membanes
Cholesterol
Dppc
title_short Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles
title_full Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles
title_fullStr Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles
title_full_unstemmed Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles
title_sort Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles
dc.creator.none.fl_str_mv Ohtake, Satoshi
Schebor, Carolina Claudia
de Pablo, Juan J.
author Ohtake, Satoshi
author_facet Ohtake, Satoshi
Schebor, Carolina Claudia
de Pablo, Juan J.
author_role author
author2 Schebor, Carolina Claudia
de Pablo, Juan J.
author2_role author
author
dc.subject.none.fl_str_mv Trehalose
Membanes
Cholesterol
Dppc
topic Trehalose
Membanes
Cholesterol
Dppc
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC–cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDSC and DSC are used to examine the phase behavior of hydrated and dehydrated vesicles, respectively. The concentration of trehalose inside and outside the vesicles is manipulated, and is shown to affect the relative stability of the membranes. Our results show for the first time that a combination of high inner and low outer trehalose concentration is able to decrease the gel-to-liquid crystalline phase temperature (Tm), while any other combination will not. Upon dehydration, the Tm of all lipid mixtures increases. The extent of the increase depends on the trehalose distribution across the bilayer. The Tm changes in the same direction with trehalose concentration for both freeze-dried and fully hydrated samples, suggesting that the trehalose distribution across the vesicle membrane, as well as the trehalose–phospholipid interaction, is maintained upon lyophilization. The results presented in this work may aid in the formulation of systems to be used in the lyophilization of liposomes for drug delivery applications.
Fil: Ohtake, Satoshi. University of Wisconsin; Estados Unidos
Fil: Schebor, Carolina Claudia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Pablo, Juan J.. University of Wisconsin; Estados Unidos
description A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC–cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDSC and DSC are used to examine the phase behavior of hydrated and dehydrated vesicles, respectively. The concentration of trehalose inside and outside the vesicles is manipulated, and is shown to affect the relative stability of the membranes. Our results show for the first time that a combination of high inner and low outer trehalose concentration is able to decrease the gel-to-liquid crystalline phase temperature (Tm), while any other combination will not. Upon dehydration, the Tm of all lipid mixtures increases. The extent of the increase depends on the trehalose distribution across the bilayer. The Tm changes in the same direction with trehalose concentration for both freeze-dried and fully hydrated samples, suggesting that the trehalose distribution across the vesicle membrane, as well as the trehalose–phospholipid interaction, is maintained upon lyophilization. The results presented in this work may aid in the formulation of systems to be used in the lyophilization of liposomes for drug delivery applications.
publishDate 2006
dc.date.none.fl_str_mv 2006-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16623
Ohtake, Satoshi; Schebor, Carolina Claudia; de Pablo, Juan J.; Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles; Elsevier Science; Biochimica Et Biophysica Acta - Biomembranes; 1758; 1; 1-2006; 65-73
0005-2736
url http://hdl.handle.net/11336/16623
identifier_str_mv Ohtake, Satoshi; Schebor, Carolina Claudia; de Pablo, Juan J.; Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles; Elsevier Science; Biochimica Et Biophysica Acta - Biomembranes; 1758; 1; 1-2006; 65-73
0005-2736
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2006.01.002
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0005273606000071
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782076366356480
score 12.982451

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