Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose

Autores
Ohtake, S.; Schebor, C.; Palecek, S.P.; De Pablo, J.J.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved.
Fil:Schebor, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Biochim. Biophys. Acta Biomembr. 2005;1713(1):57-64
Materia
Cholesterol
DPPC
DPPE
Freeze-dried
Phase transition
cholesterol
dipalmitoylphosphatidylcholine
dipalmitoylphosphatidylethanolamine
liposome
phospholipid
trehalose
article
enthalpy
freeze drying
lipid composition
lipid membrane
phase separation
phase transition
priority journal
temperature sensitivity
thermostability
1,2-Dipalmitoylphosphatidylcholine
Calorimetry, Differential Scanning
Cholesterol
Freeze Drying
Phosphatidylethanolamines
Trehalose
Mammalia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00052736_v1713_n1_p57_Ohtake
Acceder
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oai_identifier_str paperaa:paper_00052736_v1713_n1_p57_Ohtake
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehaloseOhtake, S.Schebor, C.Palecek, S.P.De Pablo, J.J.CholesterolDPPCDPPEFreeze-driedPhase transitioncholesteroldipalmitoylphosphatidylcholinedipalmitoylphosphatidylethanolamineliposomephospholipidtrehalosearticleenthalpyfreeze dryinglipid compositionlipid membranephase separationphase transitionpriority journaltemperature sensitivitythermostability1,2-DipalmitoylphosphatidylcholineCalorimetry, Differential ScanningCholesterolFreeze DryingPhosphatidylethanolaminesTrehaloseMammaliaA study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved.Fil:Schebor, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_OhtakeBiochim. Biophys. Acta Biomembr. 2005;1713(1):57-64reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-11-13T08:45:32Zpaperaa:paper_00052736_v1713_n1_p57_OhtakeInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-11-13 08:45:33.5Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
spellingShingle Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
Ohtake, S.
Cholesterol
DPPC
DPPE
Freeze-dried
Phase transition
cholesterol
dipalmitoylphosphatidylcholine
dipalmitoylphosphatidylethanolamine
liposome
phospholipid
trehalose
article
enthalpy
freeze drying
lipid composition
lipid membrane
phase separation
phase transition
priority journal
temperature sensitivity
thermostability
1,2-Dipalmitoylphosphatidylcholine
Calorimetry, Differential Scanning
Cholesterol
Freeze Drying
Phosphatidylethanolamines
Trehalose
Mammalia
title_short Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_full Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_fullStr Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_full_unstemmed Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
title_sort Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
dc.creator.none.fl_str_mv Ohtake, S.
Schebor, C.
Palecek, S.P.
De Pablo, J.J.
author Ohtake, S.
author_facet Ohtake, S.
Schebor, C.
Palecek, S.P.
De Pablo, J.J.
author_role author
author2 Schebor, C.
Palecek, S.P.
De Pablo, J.J.
author2_role author
author
author
dc.subject.none.fl_str_mv Cholesterol
DPPC
DPPE
Freeze-dried
Phase transition
cholesterol
dipalmitoylphosphatidylcholine
dipalmitoylphosphatidylethanolamine
liposome
phospholipid
trehalose
article
enthalpy
freeze drying
lipid composition
lipid membrane
phase separation
phase transition
priority journal
temperature sensitivity
thermostability
1,2-Dipalmitoylphosphatidylcholine
Calorimetry, Differential Scanning
Cholesterol
Freeze Drying
Phosphatidylethanolamines
Trehalose
Mammalia
topic Cholesterol
DPPC
DPPE
Freeze-dried
Phase transition
cholesterol
dipalmitoylphosphatidylcholine
dipalmitoylphosphatidylethanolamine
liposome
phospholipid
trehalose
article
enthalpy
freeze drying
lipid composition
lipid membrane
phase separation
phase transition
priority journal
temperature sensitivity
thermostability
1,2-Dipalmitoylphosphatidylcholine
Calorimetry, Differential Scanning
Cholesterol
Freeze Drying
Phosphatidylethanolamines
Trehalose
Mammalia
dc.description.none.fl_txt_mv A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved.
Fil:Schebor, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake
url http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biochim. Biophys. Acta Biomembr. 2005;1713(1):57-64
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
_version_ 1848680049879810048
score 12.742515

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