How low does the oxygen concentration go within a sandwich-type amperometric biosensor?

Autores
Romero, Marcelo Ricardo; Baruzzi, Ana Maria; Garay, Fernando Sebastian
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Software for the automatic non-linear least squares fit of chronoamperometric responses corresponding to sandwich-type amperometric biosensors has been developed. The so-called Simplex algorithm computes a minimum value for the difference between experimental and theoretical data. The latter consider a numerical model based on a ping-pong reaction mechanism corresponding to an oxidase enzyme that has been immobilized between diffusion membranes. The results obtained from the simulation of a first-generation lactate biosensor in presence of 0.1 mM substrate indicate that the concentration of O 2 would decrease only 0.1% with regards to its bulk value. Besides, the concentration of this natural mediator would remain practically unchanged during a typical calibration curve. This is because the rather high diffusion coefficient of O 2 and its regeneration at the electrode surface minimize the concentration changes of this species. In addition, it was found that the thicknesses of polycarbonate membranes and the enzymatic matrix have average values of 13 μm and 20 μm, respectively. However, these membranes might exhibit smaller thickness depending on the time provided for the crosslinking reaction. In this regard, if this reaction is slow enough, the enzymatic matrix would be able to diffuse through the pores of polycarbonate membranes and they will appear to be thinner than expected. This effect may compromise the response-time and the reproducibility of this kind of biosensors.
Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
AMPEROMETRIC SENSOR
HYDROGEL
OXIDASE
PING-PONG
SANDWICH-TYPE BIOSENSOR
SIMPLEX
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113315
Acceder
id CONICETDig_8135c19b812c69f62d87296fb1232850
oai_identifier_str oai:ri.conicet.gov.ar:11336/113315
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling How low does the oxygen concentration go within a sandwich-type amperometric biosensor?Romero, Marcelo RicardoBaruzzi, Ana MariaGaray, Fernando SebastianAMPEROMETRIC SENSORHYDROGELOXIDASEPING-PONGSANDWICH-TYPE BIOSENSORSIMPLEXhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Software for the automatic non-linear least squares fit of chronoamperometric responses corresponding to sandwich-type amperometric biosensors has been developed. The so-called Simplex algorithm computes a minimum value for the difference between experimental and theoretical data. The latter consider a numerical model based on a ping-pong reaction mechanism corresponding to an oxidase enzyme that has been immobilized between diffusion membranes. The results obtained from the simulation of a first-generation lactate biosensor in presence of 0.1 mM substrate indicate that the concentration of O 2 would decrease only 0.1% with regards to its bulk value. Besides, the concentration of this natural mediator would remain practically unchanged during a typical calibration curve. This is because the rather high diffusion coefficient of O 2 and its regeneration at the electrode surface minimize the concentration changes of this species. In addition, it was found that the thicknesses of polycarbonate membranes and the enzymatic matrix have average values of 13 μm and 20 μm, respectively. However, these membranes might exhibit smaller thickness depending on the time provided for the crosslinking reaction. In this regard, if this reaction is slow enough, the enzymatic matrix would be able to diffuse through the pores of polycarbonate membranes and they will appear to be thinner than expected. This effect may compromise the response-time and the reproducibility of this kind of biosensors.Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science Sa2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113315Romero, Marcelo Ricardo; Baruzzi, Ana Maria; Garay, Fernando Sebastian; How low does the oxygen concentration go within a sandwich-type amperometric biosensor?; Elsevier Science Sa; Sensors and Actuators B: Chemical; 174; 11-2012; 279-2840925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400512008416#info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2012.08.027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:33:39Zoai:ri.conicet.gov.ar:11336/113315instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:33:39.319CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv How low does the oxygen concentration go within a sandwich-type amperometric biosensor?
title How low does the oxygen concentration go within a sandwich-type amperometric biosensor?
spellingShingle How low does the oxygen concentration go within a sandwich-type amperometric biosensor?
Romero, Marcelo Ricardo
AMPEROMETRIC SENSOR
HYDROGEL
OXIDASE
PING-PONG
SANDWICH-TYPE BIOSENSOR
SIMPLEX
title_short How low does the oxygen concentration go within a sandwich-type amperometric biosensor?
title_full How low does the oxygen concentration go within a sandwich-type amperometric biosensor?
title_fullStr How low does the oxygen concentration go within a sandwich-type amperometric biosensor?
title_full_unstemmed How low does the oxygen concentration go within a sandwich-type amperometric biosensor?
title_sort How low does the oxygen concentration go within a sandwich-type amperometric biosensor?
dc.creator.none.fl_str_mv Romero, Marcelo Ricardo
Baruzzi, Ana Maria
Garay, Fernando Sebastian
author Romero, Marcelo Ricardo
author_facet Romero, Marcelo Ricardo
Baruzzi, Ana Maria
Garay, Fernando Sebastian
author_role author
author2 Baruzzi, Ana Maria
Garay, Fernando Sebastian
author2_role author
author
dc.subject.none.fl_str_mv AMPEROMETRIC SENSOR
HYDROGEL
OXIDASE
PING-PONG
SANDWICH-TYPE BIOSENSOR
SIMPLEX
topic AMPEROMETRIC SENSOR
HYDROGEL
OXIDASE
PING-PONG
SANDWICH-TYPE BIOSENSOR
SIMPLEX
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Software for the automatic non-linear least squares fit of chronoamperometric responses corresponding to sandwich-type amperometric biosensors has been developed. The so-called Simplex algorithm computes a minimum value for the difference between experimental and theoretical data. The latter consider a numerical model based on a ping-pong reaction mechanism corresponding to an oxidase enzyme that has been immobilized between diffusion membranes. The results obtained from the simulation of a first-generation lactate biosensor in presence of 0.1 mM substrate indicate that the concentration of O 2 would decrease only 0.1% with regards to its bulk value. Besides, the concentration of this natural mediator would remain practically unchanged during a typical calibration curve. This is because the rather high diffusion coefficient of O 2 and its regeneration at the electrode surface minimize the concentration changes of this species. In addition, it was found that the thicknesses of polycarbonate membranes and the enzymatic matrix have average values of 13 μm and 20 μm, respectively. However, these membranes might exhibit smaller thickness depending on the time provided for the crosslinking reaction. In this regard, if this reaction is slow enough, the enzymatic matrix would be able to diffuse through the pores of polycarbonate membranes and they will appear to be thinner than expected. This effect may compromise the response-time and the reproducibility of this kind of biosensors.
Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description Software for the automatic non-linear least squares fit of chronoamperometric responses corresponding to sandwich-type amperometric biosensors has been developed. The so-called Simplex algorithm computes a minimum value for the difference between experimental and theoretical data. The latter consider a numerical model based on a ping-pong reaction mechanism corresponding to an oxidase enzyme that has been immobilized between diffusion membranes. The results obtained from the simulation of a first-generation lactate biosensor in presence of 0.1 mM substrate indicate that the concentration of O 2 would decrease only 0.1% with regards to its bulk value. Besides, the concentration of this natural mediator would remain practically unchanged during a typical calibration curve. This is because the rather high diffusion coefficient of O 2 and its regeneration at the electrode surface minimize the concentration changes of this species. In addition, it was found that the thicknesses of polycarbonate membranes and the enzymatic matrix have average values of 13 μm and 20 μm, respectively. However, these membranes might exhibit smaller thickness depending on the time provided for the crosslinking reaction. In this regard, if this reaction is slow enough, the enzymatic matrix would be able to diffuse through the pores of polycarbonate membranes and they will appear to be thinner than expected. This effect may compromise the response-time and the reproducibility of this kind of biosensors.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113315
Romero, Marcelo Ricardo; Baruzzi, Ana Maria; Garay, Fernando Sebastian; How low does the oxygen concentration go within a sandwich-type amperometric biosensor?; Elsevier Science Sa; Sensors and Actuators B: Chemical; 174; 11-2012; 279-284
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113315
identifier_str_mv Romero, Marcelo Ricardo; Baruzzi, Ana Maria; Garay, Fernando Sebastian; How low does the oxygen concentration go within a sandwich-type amperometric biosensor?; Elsevier Science Sa; Sensors and Actuators B: Chemical; 174; 11-2012; 279-284
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400512008416#
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2012.08.027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847427302669942784
score 13.10058

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