Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)

Autores
Assef, Yanina Andrea; Horak, Cristina Natalia; Boquet, M. G.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
P-glycoprotein (P-gp) mediated multixenobiotic resistance (MXR) is a mechanism analogous to multidrug resistance (MDR), which has been extensively characterised in mammalian tumours. The expression and function of the MXR mechanism has been demonstrated in numerous aquatic organisms and has been proposed as a biomarker for pollution assessment. The purpose of this study was to evaluate the activity and expression of the MXR mechanism in freshwater snails (Physa acuta) of the Andean–Patagonian region. The accumulation and efflux rates of the model P-gp substrate Rhodamine B in snails previously exposed to pollution were measured. Results confirmed that MXR activity decreased after maintenance in clean water, and that a depuration period of 7 d was long enough to observe a significant deinduction of this system. The results demonstrate the presence of a P-gp-like transport system in the freshwater snail P. acuta from Patagonia, and suggest its function as a defence system. The results of this study could be used to provide information on the possible use of these snails as bioindicators in toxicological testing.
Fil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina
Fil: Horak, Cristina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina
Fil: Boquet, M. G.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina
Materia
Mxr
P-Glycoprotein
Freshwater Snails
Patagonia
Bioindicators
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17871
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)Assef, Yanina AndreaHorak, Cristina NataliaBoquet, M. G.MxrP-GlycoproteinFreshwater SnailsPatagoniaBioindicatorshttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1P-glycoprotein (P-gp) mediated multixenobiotic resistance (MXR) is a mechanism analogous to multidrug resistance (MDR), which has been extensively characterised in mammalian tumours. The expression and function of the MXR mechanism has been demonstrated in numerous aquatic organisms and has been proposed as a biomarker for pollution assessment. The purpose of this study was to evaluate the activity and expression of the MXR mechanism in freshwater snails (Physa acuta) of the Andean–Patagonian region. The accumulation and efflux rates of the model P-gp substrate Rhodamine B in snails previously exposed to pollution were measured. Results confirmed that MXR activity decreased after maintenance in clean water, and that a depuration period of 7 d was long enough to observe a significant deinduction of this system. The results demonstrate the presence of a P-gp-like transport system in the freshwater snail P. acuta from Patagonia, and suggest its function as a defence system. The results of this study could be used to provide information on the possible use of these snails as bioindicators in toxicological testing.Fil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; ArgentinaFil: Horak, Cristina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; ArgentinaFil: Boquet, M. G.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; ArgentinaTaylor & Francis2013-11-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17871Assef, Yanina Andrea; Horak, Cristina Natalia; Boquet, M. G.; Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina); Taylor & Francis; New Zealand Journal Of Marine And Freshwater Research; 48; 1; 13-11-2013; 86-960028-83301175-8805enginfo:eu-repo/semantics/altIdentifier/doi/10.1080/00288330.2013.846921info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/00288330.2013.846921info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:40Zoai:ri.conicet.gov.ar:11336/17871instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:41.069CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)
title Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)
spellingShingle Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)
Assef, Yanina Andrea
Mxr
P-Glycoprotein
Freshwater Snails
Patagonia
Bioindicators
title_short Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)
title_full Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)
title_fullStr Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)
title_full_unstemmed Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)
title_sort Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina)
dc.creator.none.fl_str_mv Assef, Yanina Andrea
Horak, Cristina Natalia
Boquet, M. G.
author Assef, Yanina Andrea
author_facet Assef, Yanina Andrea
Horak, Cristina Natalia
Boquet, M. G.
author_role author
author2 Horak, Cristina Natalia
Boquet, M. G.
author2_role author
author
dc.subject.none.fl_str_mv Mxr
P-Glycoprotein
Freshwater Snails
Patagonia
Bioindicators
topic Mxr
P-Glycoprotein
Freshwater Snails
Patagonia
Bioindicators
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv P-glycoprotein (P-gp) mediated multixenobiotic resistance (MXR) is a mechanism analogous to multidrug resistance (MDR), which has been extensively characterised in mammalian tumours. The expression and function of the MXR mechanism has been demonstrated in numerous aquatic organisms and has been proposed as a biomarker for pollution assessment. The purpose of this study was to evaluate the activity and expression of the MXR mechanism in freshwater snails (Physa acuta) of the Andean–Patagonian region. The accumulation and efflux rates of the model P-gp substrate Rhodamine B in snails previously exposed to pollution were measured. Results confirmed that MXR activity decreased after maintenance in clean water, and that a depuration period of 7 d was long enough to observe a significant deinduction of this system. The results demonstrate the presence of a P-gp-like transport system in the freshwater snail P. acuta from Patagonia, and suggest its function as a defence system. The results of this study could be used to provide information on the possible use of these snails as bioindicators in toxicological testing.
Fil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina
Fil: Horak, Cristina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina
Fil: Boquet, M. G.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentina
description P-glycoprotein (P-gp) mediated multixenobiotic resistance (MXR) is a mechanism analogous to multidrug resistance (MDR), which has been extensively characterised in mammalian tumours. The expression and function of the MXR mechanism has been demonstrated in numerous aquatic organisms and has been proposed as a biomarker for pollution assessment. The purpose of this study was to evaluate the activity and expression of the MXR mechanism in freshwater snails (Physa acuta) of the Andean–Patagonian region. The accumulation and efflux rates of the model P-gp substrate Rhodamine B in snails previously exposed to pollution were measured. Results confirmed that MXR activity decreased after maintenance in clean water, and that a depuration period of 7 d was long enough to observe a significant deinduction of this system. The results demonstrate the presence of a P-gp-like transport system in the freshwater snail P. acuta from Patagonia, and suggest its function as a defence system. The results of this study could be used to provide information on the possible use of these snails as bioindicators in toxicological testing.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17871
Assef, Yanina Andrea; Horak, Cristina Natalia; Boquet, M. G.; Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina); Taylor & Francis; New Zealand Journal Of Marine And Freshwater Research; 48; 1; 13-11-2013; 86-96
0028-8330
1175-8805
url http://hdl.handle.net/11336/17871
identifier_str_mv Assef, Yanina Andrea; Horak, Cristina Natalia; Boquet, M. G.; Characterisation of the multixenobiotic resistance (MXR) mechanism in the freshwater snail Physa acuta from Patagonia (Argentina); Taylor & Francis; New Zealand Journal Of Marine And Freshwater Research; 48; 1; 13-11-2013; 86-96
0028-8330
1175-8805
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/00288330.2013.846921
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/00288330.2013.846921
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613535727878144
score 13.070432

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