Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples

Autores
Cabas Rodríguez, José Alberto; Arevalo, Fernando Javier; Granero, Adrian Marcelo
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paracetamol (PAR) is a common antipyretic and analgesic extensively used to treat cold and flu symptoms. It has been proven to be effective in headaches and relieving fever and pain. It is usually found as an over-the-counter drug, which has been associated with an increase in cases of poisoning due to overdose. Therefore, the development of new analytical tools for the detection of PAR at low concentrations in different samples is necessary. In this work, a Molecularly Imprinted Polymer (MIP)-based electrochemical sensor was designed for the selective and sensitive determination of PAR using a glassy carbon electrode (GCE) modified with a polymeric film obtained through the electropolymerization of o-aminophenol. A complete characterization based on electrochemical techniques, such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and scanning electron microscopy (SEM) was used to examine all steps involved in the construction of the MIP-based electrochemical sensor. In addition, all parameters affecting the MIP were optimized. As a result, the MIP-based electrochemical sensor showed a very low limit of detection (LOD) of 10 nM, with an analytical sensitivity of (3.4 ± 0.1) A M−1. In addition, construction of the MIP-based electrochemical sensor showed highly reproducibility, expressed in terms of a variation coefficient lower than 4%. The MIP-based electrochemical sensor was successfully used in an assay for the determination of PAR in pharmaceutical products. The performance of the MIP-based electrochemical sensor was compared to High Performance Liquid Chromatography (HPLC) for the determination of PAR in pharmaceutical samples, showing excellent agreement between the two methodologies. A very important aspect of the developed sensor was its reusability for at least twenty times. The MIP-based electrochemical sensor is a reliable analytical tool for the determination of PAR.
Fil: Cabas Rodríguez, José Alberto. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Materia
ELECTROCHEMICAL SENSOR
PHARMACEUTICAL SAMPLES
PARACETAMOL
MOLECULARLY IMPRINTED POLYMER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281917
Acceder
id CONICETDig_e6ab0f20bd6ed7ab588a0e973f583536
oai_identifier_str oai:ri.conicet.gov.ar:11336/281917
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical SamplesCabas Rodríguez, José AlbertoArevalo, Fernando JavierGranero, Adrian MarceloELECTROCHEMICAL SENSORPHARMACEUTICAL SAMPLESPARACETAMOLMOLECULARLY IMPRINTED POLYMERhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Paracetamol (PAR) is a common antipyretic and analgesic extensively used to treat cold and flu symptoms. It has been proven to be effective in headaches and relieving fever and pain. It is usually found as an over-the-counter drug, which has been associated with an increase in cases of poisoning due to overdose. Therefore, the development of new analytical tools for the detection of PAR at low concentrations in different samples is necessary. In this work, a Molecularly Imprinted Polymer (MIP)-based electrochemical sensor was designed for the selective and sensitive determination of PAR using a glassy carbon electrode (GCE) modified with a polymeric film obtained through the electropolymerization of o-aminophenol. A complete characterization based on electrochemical techniques, such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and scanning electron microscopy (SEM) was used to examine all steps involved in the construction of the MIP-based electrochemical sensor. In addition, all parameters affecting the MIP were optimized. As a result, the MIP-based electrochemical sensor showed a very low limit of detection (LOD) of 10 nM, with an analytical sensitivity of (3.4 ± 0.1) A M−1. In addition, construction of the MIP-based electrochemical sensor showed highly reproducibility, expressed in terms of a variation coefficient lower than 4%. The MIP-based electrochemical sensor was successfully used in an assay for the determination of PAR in pharmaceutical products. The performance of the MIP-based electrochemical sensor was compared to High Performance Liquid Chromatography (HPLC) for the determination of PAR in pharmaceutical samples, showing excellent agreement between the two methodologies. A very important aspect of the developed sensor was its reusability for at least twenty times. The MIP-based electrochemical sensor is a reliable analytical tool for the determination of PAR.Fil: Cabas Rodríguez, José Alberto. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaMultidisciplinary Digital Publishing Institute2025-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281917Cabas Rodríguez, José Alberto; Arevalo, Fernando Javier; Granero, Adrian Marcelo; Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples; Multidisciplinary Digital Publishing Institute; Biosensors; 15; 8; 8-2025; 1-172079-6374CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6374/15/8/544info:eu-repo/semantics/altIdentifier/doi/10.3390/bios15080544info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-04-23T14:14:54Zoai:ri.conicet.gov.ar:11336/281917instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-04-23 14:14:55.477CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples
title Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples
spellingShingle Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples
Cabas Rodríguez, José Alberto
ELECTROCHEMICAL SENSOR
PHARMACEUTICAL SAMPLES
PARACETAMOL
MOLECULARLY IMPRINTED POLYMER
title_short Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples
title_full Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples
title_fullStr Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples
title_full_unstemmed Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples
title_sort Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples
dc.creator.none.fl_str_mv Cabas Rodríguez, José Alberto
Arevalo, Fernando Javier
Granero, Adrian Marcelo
author Cabas Rodríguez, José Alberto
author_facet Cabas Rodríguez, José Alberto
Arevalo, Fernando Javier
Granero, Adrian Marcelo
author_role author
author2 Arevalo, Fernando Javier
Granero, Adrian Marcelo
author2_role author
author
dc.subject.none.fl_str_mv ELECTROCHEMICAL SENSOR
PHARMACEUTICAL SAMPLES
PARACETAMOL
MOLECULARLY IMPRINTED POLYMER
topic ELECTROCHEMICAL SENSOR
PHARMACEUTICAL SAMPLES
PARACETAMOL
MOLECULARLY IMPRINTED POLYMER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Paracetamol (PAR) is a common antipyretic and analgesic extensively used to treat cold and flu symptoms. It has been proven to be effective in headaches and relieving fever and pain. It is usually found as an over-the-counter drug, which has been associated with an increase in cases of poisoning due to overdose. Therefore, the development of new analytical tools for the detection of PAR at low concentrations in different samples is necessary. In this work, a Molecularly Imprinted Polymer (MIP)-based electrochemical sensor was designed for the selective and sensitive determination of PAR using a glassy carbon electrode (GCE) modified with a polymeric film obtained through the electropolymerization of o-aminophenol. A complete characterization based on electrochemical techniques, such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and scanning electron microscopy (SEM) was used to examine all steps involved in the construction of the MIP-based electrochemical sensor. In addition, all parameters affecting the MIP were optimized. As a result, the MIP-based electrochemical sensor showed a very low limit of detection (LOD) of 10 nM, with an analytical sensitivity of (3.4 ± 0.1) A M−1. In addition, construction of the MIP-based electrochemical sensor showed highly reproducibility, expressed in terms of a variation coefficient lower than 4%. The MIP-based electrochemical sensor was successfully used in an assay for the determination of PAR in pharmaceutical products. The performance of the MIP-based electrochemical sensor was compared to High Performance Liquid Chromatography (HPLC) for the determination of PAR in pharmaceutical samples, showing excellent agreement between the two methodologies. A very important aspect of the developed sensor was its reusability for at least twenty times. The MIP-based electrochemical sensor is a reliable analytical tool for the determination of PAR.
Fil: Cabas Rodríguez, José Alberto. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
description Paracetamol (PAR) is a common antipyretic and analgesic extensively used to treat cold and flu symptoms. It has been proven to be effective in headaches and relieving fever and pain. It is usually found as an over-the-counter drug, which has been associated with an increase in cases of poisoning due to overdose. Therefore, the development of new analytical tools for the detection of PAR at low concentrations in different samples is necessary. In this work, a Molecularly Imprinted Polymer (MIP)-based electrochemical sensor was designed for the selective and sensitive determination of PAR using a glassy carbon electrode (GCE) modified with a polymeric film obtained through the electropolymerization of o-aminophenol. A complete characterization based on electrochemical techniques, such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and scanning electron microscopy (SEM) was used to examine all steps involved in the construction of the MIP-based electrochemical sensor. In addition, all parameters affecting the MIP were optimized. As a result, the MIP-based electrochemical sensor showed a very low limit of detection (LOD) of 10 nM, with an analytical sensitivity of (3.4 ± 0.1) A M−1. In addition, construction of the MIP-based electrochemical sensor showed highly reproducibility, expressed in terms of a variation coefficient lower than 4%. The MIP-based electrochemical sensor was successfully used in an assay for the determination of PAR in pharmaceutical products. The performance of the MIP-based electrochemical sensor was compared to High Performance Liquid Chromatography (HPLC) for the determination of PAR in pharmaceutical samples, showing excellent agreement between the two methodologies. A very important aspect of the developed sensor was its reusability for at least twenty times. The MIP-based electrochemical sensor is a reliable analytical tool for the determination of PAR.
publishDate 2025
dc.date.none.fl_str_mv 2025-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281917
Cabas Rodríguez, José Alberto; Arevalo, Fernando Javier; Granero, Adrian Marcelo; Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples; Multidisciplinary Digital Publishing Institute; Biosensors; 15; 8; 8-2025; 1-17
2079-6374
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281917
identifier_str_mv Cabas Rodríguez, José Alberto; Arevalo, Fernando Javier; Granero, Adrian Marcelo; Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples; Multidisciplinary Digital Publishing Institute; Biosensors; 15; 8; 8-2025; 1-17
2079-6374
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6374/15/8/544
info:eu-repo/semantics/altIdentifier/doi/10.3390/bios15080544
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1863364478955945984
score 13.05261

Publicaciones similares