ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones

Autores
Glisoni, Romina Julieta; García Fernández, María J.; Pino, Marylú; Gutkind, Gabriel Osvaldo; Moglioni, Albertina Gladys; Alvarez Lorenzo, Carmen; Concheiro, Angel; Sosnik, Alejandro Dario
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two types of hydrophilic networks with conjugated beta cyclodextrin (b-CD) were developed with the aim of engineering useful platforms for the localized release of an antimicrobial  5,6-dimethoxy-1-indanone N4-allyl thiosemicarbazone (TSC) in the eye and its potential application in ophthalmic diseases. Poly(2-hydroxyethyl methacrylate) soft contact lenses (SCLs) displaying pendant β-CD, namely pHEMA-co-β-CD, and super-hydrophilic hydrogels (SHHs) of directly cross-linked hydroxypropyl-β-CD were synthesized and characterized regarding their structure (ATR/FTIR), drug loading capacity, swelling and in vitro release in artificial lacrimal fluid. Incorporation of TSC to the networks was carried out both during polymerization (DP method) and after synthesis (PP method). The first method led to similar drug loads in all the hydrogels, with minor drug loss during the washing steps to remove unreacted monomers, while the second method evidenced the influence of structural parameters on the loading efficiency (proportion of CD units, mesh size, swelling degree). Both systems provided a controlled TSC release for at least two weeks, TSC concentrations (up to 4000ug/g dry hydrogel) being within an optimal therapeutic window for the antimicrobial ocular treatment. Microbiological tests against P. aeruginosa and S. aureus confirmed the ability of TSC-loaded pHEMAco-β-CD network to inhibit bacterial growth.
Fil: Glisoni, Romina Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García Fernández, María J.. Universidad de Santiago de Compostela; España;
Fil: Pino, Marylú. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moglioni, Albertina Gladys. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez Lorenzo, Carmen. Universidad de Santiago de Compostela; España;
Fil: Concheiro, Angel. Universidad de Santiago de Compostela; España;
Fil: Sosnik, Alejandro Dario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
2-Hydroxyethyl-Methacrylate
Medicated Soft Contact Lenses
Superhydrophilic Networks
N Allyl Thiosemicarbazone
Cyclodextrin Complexation
Antibacterial Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1770
Acceder
id CONICETDig_e42e2527096672ceb72ecc419e556ed9
oai_identifier_str oai:ri.conicet.gov.ar:11336/1770
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazonesGlisoni, Romina JulietaGarcía Fernández, María J.Pino, MarylúGutkind, Gabriel OsvaldoMoglioni, Albertina GladysAlvarez Lorenzo, CarmenConcheiro, AngelSosnik, Alejandro Dario2-Hydroxyethyl-MethacrylateMedicated Soft Contact LensesSuperhydrophilic NetworksN Allyl ThiosemicarbazoneCyclodextrin ComplexationAntibacterial Activityhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Two types of hydrophilic networks with conjugated beta cyclodextrin (b-CD) were developed with the aim of engineering useful platforms for the localized release of an antimicrobial  5,6-dimethoxy-1-indanone N4-allyl thiosemicarbazone (TSC) in the eye and its potential application in ophthalmic diseases. Poly(2-hydroxyethyl methacrylate) soft contact lenses (SCLs) displaying pendant β-CD, namely pHEMA-co-β-CD, and super-hydrophilic hydrogels (SHHs) of directly cross-linked hydroxypropyl-β-CD were synthesized and characterized regarding their structure (ATR/FTIR), drug loading capacity, swelling and in vitro release in artificial lacrimal fluid. Incorporation of TSC to the networks was carried out both during polymerization (DP method) and after synthesis (PP method). The first method led to similar drug loads in all the hydrogels, with minor drug loss during the washing steps to remove unreacted monomers, while the second method evidenced the influence of structural parameters on the loading efficiency (proportion of CD units, mesh size, swelling degree). Both systems provided a controlled TSC release for at least two weeks, TSC concentrations (up to 4000ug/g dry hydrogel) being within an optimal therapeutic window for the antimicrobial ocular treatment. Microbiological tests against P. aeruginosa and S. aureus confirmed the ability of TSC-loaded pHEMAco-β-CD network to inhibit bacterial growth.Fil: Glisoni, Romina Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: García Fernández, María J.. Universidad de Santiago de Compostela; España;Fil: Pino, Marylú. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; ArgentinaFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moglioni, Albertina Gladys. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez Lorenzo, Carmen. Universidad de Santiago de Compostela; España;Fil: Concheiro, Angel. Universidad de Santiago de Compostela; España;Fil: Sosnik, Alejandro Dario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-04-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1770Glisoni, Romina Julieta; García Fernández, María J.; Pino, Marylú; Gutkind, Gabriel Osvaldo; Moglioni, Albertina Gladys; et al.; ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones; Elsevier; Carbohydrate Polymers; 93; 2; 2-4-2013; 449-4570144-8617enginfo:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.carbpol.2012.12.033info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0144861712012568info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:50:20Zoai:ri.conicet.gov.ar:11336/1770instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:50:20.208CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones
title ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones
spellingShingle ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones
Glisoni, Romina Julieta
2-Hydroxyethyl-Methacrylate
Medicated Soft Contact Lenses
Superhydrophilic Networks
N Allyl Thiosemicarbazone
Cyclodextrin Complexation
Antibacterial Activity
title_short ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones
title_full ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones
title_fullStr ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones
title_full_unstemmed ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones
title_sort ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones
dc.creator.none.fl_str_mv Glisoni, Romina Julieta
García Fernández, María J.
Pino, Marylú
Gutkind, Gabriel Osvaldo
Moglioni, Albertina Gladys
Alvarez Lorenzo, Carmen
Concheiro, Angel
Sosnik, Alejandro Dario
author Glisoni, Romina Julieta
author_facet Glisoni, Romina Julieta
García Fernández, María J.
Pino, Marylú
Gutkind, Gabriel Osvaldo
Moglioni, Albertina Gladys
Alvarez Lorenzo, Carmen
Concheiro, Angel
Sosnik, Alejandro Dario
author_role author
author2 García Fernández, María J.
Pino, Marylú
Gutkind, Gabriel Osvaldo
Moglioni, Albertina Gladys
Alvarez Lorenzo, Carmen
Concheiro, Angel
Sosnik, Alejandro Dario
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 2-Hydroxyethyl-Methacrylate
Medicated Soft Contact Lenses
Superhydrophilic Networks
N Allyl Thiosemicarbazone
Cyclodextrin Complexation
Antibacterial Activity
topic 2-Hydroxyethyl-Methacrylate
Medicated Soft Contact Lenses
Superhydrophilic Networks
N Allyl Thiosemicarbazone
Cyclodextrin Complexation
Antibacterial Activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Two types of hydrophilic networks with conjugated beta cyclodextrin (b-CD) were developed with the aim of engineering useful platforms for the localized release of an antimicrobial  5,6-dimethoxy-1-indanone N4-allyl thiosemicarbazone (TSC) in the eye and its potential application in ophthalmic diseases. Poly(2-hydroxyethyl methacrylate) soft contact lenses (SCLs) displaying pendant β-CD, namely pHEMA-co-β-CD, and super-hydrophilic hydrogels (SHHs) of directly cross-linked hydroxypropyl-β-CD were synthesized and characterized regarding their structure (ATR/FTIR), drug loading capacity, swelling and in vitro release in artificial lacrimal fluid. Incorporation of TSC to the networks was carried out both during polymerization (DP method) and after synthesis (PP method). The first method led to similar drug loads in all the hydrogels, with minor drug loss during the washing steps to remove unreacted monomers, while the second method evidenced the influence of structural parameters on the loading efficiency (proportion of CD units, mesh size, swelling degree). Both systems provided a controlled TSC release for at least two weeks, TSC concentrations (up to 4000ug/g dry hydrogel) being within an optimal therapeutic window for the antimicrobial ocular treatment. Microbiological tests against P. aeruginosa and S. aureus confirmed the ability of TSC-loaded pHEMAco-β-CD network to inhibit bacterial growth.
Fil: Glisoni, Romina Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García Fernández, María J.. Universidad de Santiago de Compostela; España;
Fil: Pino, Marylú. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moglioni, Albertina Gladys. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez Lorenzo, Carmen. Universidad de Santiago de Compostela; España;
Fil: Concheiro, Angel. Universidad de Santiago de Compostela; España;
Fil: Sosnik, Alejandro Dario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Two types of hydrophilic networks with conjugated beta cyclodextrin (b-CD) were developed with the aim of engineering useful platforms for the localized release of an antimicrobial  5,6-dimethoxy-1-indanone N4-allyl thiosemicarbazone (TSC) in the eye and its potential application in ophthalmic diseases. Poly(2-hydroxyethyl methacrylate) soft contact lenses (SCLs) displaying pendant β-CD, namely pHEMA-co-β-CD, and super-hydrophilic hydrogels (SHHs) of directly cross-linked hydroxypropyl-β-CD were synthesized and characterized regarding their structure (ATR/FTIR), drug loading capacity, swelling and in vitro release in artificial lacrimal fluid. Incorporation of TSC to the networks was carried out both during polymerization (DP method) and after synthesis (PP method). The first method led to similar drug loads in all the hydrogels, with minor drug loss during the washing steps to remove unreacted monomers, while the second method evidenced the influence of structural parameters on the loading efficiency (proportion of CD units, mesh size, swelling degree). Both systems provided a controlled TSC release for at least two weeks, TSC concentrations (up to 4000ug/g dry hydrogel) being within an optimal therapeutic window for the antimicrobial ocular treatment. Microbiological tests against P. aeruginosa and S. aureus confirmed the ability of TSC-loaded pHEMAco-β-CD network to inhibit bacterial growth.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1770
Glisoni, Romina Julieta; García Fernández, María J.; Pino, Marylú; Gutkind, Gabriel Osvaldo; Moglioni, Albertina Gladys; et al.; ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones; Elsevier; Carbohydrate Polymers; 93; 2; 2-4-2013; 449-457
0144-8617
url http://hdl.handle.net/11336/1770
identifier_str_mv Glisoni, Romina Julieta; García Fernández, María J.; Pino, Marylú; Gutkind, Gabriel Osvaldo; Moglioni, Albertina Gladys; et al.; ß-Cyclodextrin hydrogels for the ocular release of antibacterial thiosemicarbazones; Elsevier; Carbohydrate Polymers; 93; 2; 2-4-2013; 449-457
0144-8617
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.carbpol.2012.12.033
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0144861712012568
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848598108217278464
score 12.976206

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