Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae

Autores
Hover, Tal; Maya, Tal; Ron, Sapir; Sandovsky, Hani; Shadkchan, Yana; Kijner, Nitzan; Mitiagin, Yulia; Fichtman, Boris; Harel, Amnon; Shanks, Robert M.Q.; Bruna, Roberto Emanuel; Garcia Vescovi, Eleonora; Osherov, Nir
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have found a remarkable capacity for the ubiquitous Gram-negative rod bacterium Serratia marcescens to migrate along and kill the mycelia of zygomycete molds. This migration was restricted to zygomycete molds and several basidiomycete species. No migration was seen on any molds of the phylum Ascomycota. S. marcescens migration did not require fungal viability or surrounding growth medium, as bacteria migrated along aerial hyphae as well. S. marcescens did not exhibit growth tropism toward zygomycete mycelium. Bacterial migration along hyphae proceeded only when the hyphae grew into the bacterial colony. S. marcescens cells initially migrated along the hyphae, forming attached microcolonies that grew and coalesced to generate a biofilm that covered and killed the mycelium. Flagellum-defective strains of S. marcescens were able to migrate along zygomycete hyphae, although they were significantly slower than the wild-type strain and were delayed in fungal killing. Bacterial attachment to the mycelium does not necessitate type 1 fimbrial adhesion, since mutants defective in this adhesin migrated equally well as or faster than the wild-type strain. Killing does not depend on the secretion of S. marcescens chitinases, as mutants in which all three chitinase genes were deleted retained wild-type killing abilities. A better understanding of the mechanisms by which S. marcescens binds to, spreads on, and kills fungal hyphae might serve as an excellent model system for such interactions in general; fungal killing could be employed in agricultural fungal biocontrol.
Fil: Hover, Tal. Tel Aviv University; Israel
Fil: Maya, Tal. Tel Aviv University; Israel
Fil: Ron, Sapir. Tel Aviv University; Israel
Fil: Sandovsky, Hani. Tel Aviv University; Israel
Fil: Shadkchan, Yana. Tel Aviv University; Israel
Fil: Kijner, Nitzan. Tel Aviv University; Israel
Fil: Mitiagin, Yulia. Tel Aviv University; Israel
Fil: Fichtman, Boris. Bar Ilan University; Israel
Fil: Harel, Amnon. Bar Ilan University; Israel
Fil: Shanks, Robert M.Q.. University of Pittsburgh; Estados Unidos
Fil: Bruna, Roberto Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Osherov, Nir. Tel Aviv University; Israel
Materia
Serratia
Fungus
Interaction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52583
Acceder
id CONICETDig_e39e70fb0124fc940a292ea172a90878
oai_identifier_str oai:ri.conicet.gov.ar:11336/52583
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphaeHover, TalMaya, TalRon, SapirSandovsky, HaniShadkchan, YanaKijner, NitzanMitiagin, YuliaFichtman, BorisHarel, AmnonShanks, Robert M.Q.Bruna, Roberto EmanuelGarcia Vescovi, EleonoraOsherov, NirSerratiaFungusInteractionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have found a remarkable capacity for the ubiquitous Gram-negative rod bacterium Serratia marcescens to migrate along and kill the mycelia of zygomycete molds. This migration was restricted to zygomycete molds and several basidiomycete species. No migration was seen on any molds of the phylum Ascomycota. S. marcescens migration did not require fungal viability or surrounding growth medium, as bacteria migrated along aerial hyphae as well. S. marcescens did not exhibit growth tropism toward zygomycete mycelium. Bacterial migration along hyphae proceeded only when the hyphae grew into the bacterial colony. S. marcescens cells initially migrated along the hyphae, forming attached microcolonies that grew and coalesced to generate a biofilm that covered and killed the mycelium. Flagellum-defective strains of S. marcescens were able to migrate along zygomycete hyphae, although they were significantly slower than the wild-type strain and were delayed in fungal killing. Bacterial attachment to the mycelium does not necessitate type 1 fimbrial adhesion, since mutants defective in this adhesin migrated equally well as or faster than the wild-type strain. Killing does not depend on the secretion of S. marcescens chitinases, as mutants in which all three chitinase genes were deleted retained wild-type killing abilities. A better understanding of the mechanisms by which S. marcescens binds to, spreads on, and kills fungal hyphae might serve as an excellent model system for such interactions in general; fungal killing could be employed in agricultural fungal biocontrol.Fil: Hover, Tal. Tel Aviv University; IsraelFil: Maya, Tal. Tel Aviv University; IsraelFil: Ron, Sapir. Tel Aviv University; IsraelFil: Sandovsky, Hani. Tel Aviv University; IsraelFil: Shadkchan, Yana. Tel Aviv University; IsraelFil: Kijner, Nitzan. Tel Aviv University; IsraelFil: Mitiagin, Yulia. Tel Aviv University; IsraelFil: Fichtman, Boris. Bar Ilan University; IsraelFil: Harel, Amnon. Bar Ilan University; IsraelFil: Shanks, Robert M.Q.. University of Pittsburgh; Estados UnidosFil: Bruna, Roberto Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Osherov, Nir. Tel Aviv University; IsraelAmerican Society for Microbiology2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52583Hover, Tal; Maya, Tal; Ron, Sapir; Sandovsky, Hani; Shadkchan, Yana; et al.; Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae; American Society for Microbiology; Applied And Environmental Microbiology; 82; 9; 5-2016; 2585-25940099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.04070-15info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/82/9/2585info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:01Zoai:ri.conicet.gov.ar:11336/52583instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:02.085CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae
title Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae
spellingShingle Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae
Hover, Tal
Serratia
Fungus
Interaction
title_short Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae
title_full Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae
title_fullStr Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae
title_full_unstemmed Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae
title_sort Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae
dc.creator.none.fl_str_mv Hover, Tal
Maya, Tal
Ron, Sapir
Sandovsky, Hani
Shadkchan, Yana
Kijner, Nitzan
Mitiagin, Yulia
Fichtman, Boris
Harel, Amnon
Shanks, Robert M.Q.
Bruna, Roberto Emanuel
Garcia Vescovi, Eleonora
Osherov, Nir
author Hover, Tal
author_facet Hover, Tal
Maya, Tal
Ron, Sapir
Sandovsky, Hani
Shadkchan, Yana
Kijner, Nitzan
Mitiagin, Yulia
Fichtman, Boris
Harel, Amnon
Shanks, Robert M.Q.
Bruna, Roberto Emanuel
Garcia Vescovi, Eleonora
Osherov, Nir
author_role author
author2 Maya, Tal
Ron, Sapir
Sandovsky, Hani
Shadkchan, Yana
Kijner, Nitzan
Mitiagin, Yulia
Fichtman, Boris
Harel, Amnon
Shanks, Robert M.Q.
Bruna, Roberto Emanuel
Garcia Vescovi, Eleonora
Osherov, Nir
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Serratia
Fungus
Interaction
topic Serratia
Fungus
Interaction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have found a remarkable capacity for the ubiquitous Gram-negative rod bacterium Serratia marcescens to migrate along and kill the mycelia of zygomycete molds. This migration was restricted to zygomycete molds and several basidiomycete species. No migration was seen on any molds of the phylum Ascomycota. S. marcescens migration did not require fungal viability or surrounding growth medium, as bacteria migrated along aerial hyphae as well. S. marcescens did not exhibit growth tropism toward zygomycete mycelium. Bacterial migration along hyphae proceeded only when the hyphae grew into the bacterial colony. S. marcescens cells initially migrated along the hyphae, forming attached microcolonies that grew and coalesced to generate a biofilm that covered and killed the mycelium. Flagellum-defective strains of S. marcescens were able to migrate along zygomycete hyphae, although they were significantly slower than the wild-type strain and were delayed in fungal killing. Bacterial attachment to the mycelium does not necessitate type 1 fimbrial adhesion, since mutants defective in this adhesin migrated equally well as or faster than the wild-type strain. Killing does not depend on the secretion of S. marcescens chitinases, as mutants in which all three chitinase genes were deleted retained wild-type killing abilities. A better understanding of the mechanisms by which S. marcescens binds to, spreads on, and kills fungal hyphae might serve as an excellent model system for such interactions in general; fungal killing could be employed in agricultural fungal biocontrol.
Fil: Hover, Tal. Tel Aviv University; Israel
Fil: Maya, Tal. Tel Aviv University; Israel
Fil: Ron, Sapir. Tel Aviv University; Israel
Fil: Sandovsky, Hani. Tel Aviv University; Israel
Fil: Shadkchan, Yana. Tel Aviv University; Israel
Fil: Kijner, Nitzan. Tel Aviv University; Israel
Fil: Mitiagin, Yulia. Tel Aviv University; Israel
Fil: Fichtman, Boris. Bar Ilan University; Israel
Fil: Harel, Amnon. Bar Ilan University; Israel
Fil: Shanks, Robert M.Q.. University of Pittsburgh; Estados Unidos
Fil: Bruna, Roberto Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Osherov, Nir. Tel Aviv University; Israel
description We have found a remarkable capacity for the ubiquitous Gram-negative rod bacterium Serratia marcescens to migrate along and kill the mycelia of zygomycete molds. This migration was restricted to zygomycete molds and several basidiomycete species. No migration was seen on any molds of the phylum Ascomycota. S. marcescens migration did not require fungal viability or surrounding growth medium, as bacteria migrated along aerial hyphae as well. S. marcescens did not exhibit growth tropism toward zygomycete mycelium. Bacterial migration along hyphae proceeded only when the hyphae grew into the bacterial colony. S. marcescens cells initially migrated along the hyphae, forming attached microcolonies that grew and coalesced to generate a biofilm that covered and killed the mycelium. Flagellum-defective strains of S. marcescens were able to migrate along zygomycete hyphae, although they were significantly slower than the wild-type strain and were delayed in fungal killing. Bacterial attachment to the mycelium does not necessitate type 1 fimbrial adhesion, since mutants defective in this adhesin migrated equally well as or faster than the wild-type strain. Killing does not depend on the secretion of S. marcescens chitinases, as mutants in which all three chitinase genes were deleted retained wild-type killing abilities. A better understanding of the mechanisms by which S. marcescens binds to, spreads on, and kills fungal hyphae might serve as an excellent model system for such interactions in general; fungal killing could be employed in agricultural fungal biocontrol.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52583
Hover, Tal; Maya, Tal; Ron, Sapir; Sandovsky, Hani; Shadkchan, Yana; et al.; Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae; American Society for Microbiology; Applied And Environmental Microbiology; 82; 9; 5-2016; 2585-2594
0099-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52583
identifier_str_mv Hover, Tal; Maya, Tal; Ron, Sapir; Sandovsky, Hani; Shadkchan, Yana; et al.; Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae; American Society for Microbiology; Applied And Environmental Microbiology; 82; 9; 5-2016; 2585-2594
0099-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.04070-15
info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/82/9/2585
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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