Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye

Autores
Spitzmaul, Guillermo Federico; German, Olga Lorena; Jentsch, Thomas
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Neuronal potassium channels KCNQ (Kv7) are important for neuron physiology because they regulate their excitability. Four of the five KCNQ channel genes are mutated in human genetic diseases. There are transgenic mice for KCNQ3, -4 and -5 channels, which we used in previous works, allowing the study of protein function and mutation-associated pathologies. Recent publications showed the expression of KCNQ2 to -5 in primate eye and also a possible link between Kcnq5 gen and refractive errors. For these reasons, we investigate the expression and function of these channels in wild-type, Kcnq4-/- and Kcnq5dn/dn mice. We found a weak labeling of KCNQ4 in retinal pigmented epithelium cells, which is enhanced in pigmented cells of ciliary body. KCNQ3 was found only in cells of the non-pigmented epitehlium of the ciliary body. Opposite to what was reported in other species, no KCNQ channel subunits were found in mouse retina. Besides, immature mouse retinal neurons in culture did not show M-like potassium currents, which are generated by KCNQ channels in neurons. Our results suggest that KCNQ channels may participate in the formation of aquous humor of the eye, providing part of the companion positive current during chloride transport trough the epithelium.
Fil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Jentsch, Thomas. Leibniz-Institut fuer Molekulare Pharmakologie; Alemania. MaxDelbrueck-Centrum fuer Molekulare Medizin; Alemania
XXIX Annual Meeting of Sociedad Argentina de Investigación en Neurociencias
Huerta Grande
Argentina
Sociedad Argentina e Investigación en Neurociencias
Materia
RETINA
KCNQ
POTASSIUM
CHANNELS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222187
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eyeSpitzmaul, Guillermo FedericoGerman, Olga LorenaJentsch, ThomasRETINAKCNQPOTASSIUMCHANNELShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Neuronal potassium channels KCNQ (Kv7) are important for neuron physiology because they regulate their excitability. Four of the five KCNQ channel genes are mutated in human genetic diseases. There are transgenic mice for KCNQ3, -4 and -5 channels, which we used in previous works, allowing the study of protein function and mutation-associated pathologies. Recent publications showed the expression of KCNQ2 to -5 in primate eye and also a possible link between Kcnq5 gen and refractive errors. For these reasons, we investigate the expression and function of these channels in wild-type, Kcnq4-/- and Kcnq5dn/dn mice. We found a weak labeling of KCNQ4 in retinal pigmented epithelium cells, which is enhanced in pigmented cells of ciliary body. KCNQ3 was found only in cells of the non-pigmented epitehlium of the ciliary body. Opposite to what was reported in other species, no KCNQ channel subunits were found in mouse retina. Besides, immature mouse retinal neurons in culture did not show M-like potassium currents, which are generated by KCNQ channels in neurons. Our results suggest that KCNQ channels may participate in the formation of aquous humor of the eye, providing part of the companion positive current during chloride transport trough the epithelium.Fil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Jentsch, Thomas. Leibniz-Institut fuer Molekulare Pharmakologie; Alemania. MaxDelbrueck-Centrum fuer Molekulare Medizin; AlemaniaXXIX Annual Meeting of Sociedad Argentina de Investigación en NeurocienciasHuerta GrandeArgentinaSociedad Argentina e Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencias2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222187Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye; XXIX Annual Meeting of Sociedad Argentina de Investigación en Neurociencias; Huerta Grande; Argentina; 2014; 97-97CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/congresos-san-2/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:03Zoai:ri.conicet.gov.ar:11336/222187instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:03.622CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye
title Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye
spellingShingle Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye
Spitzmaul, Guillermo Federico
RETINA
KCNQ
POTASSIUM
CHANNELS
title_short Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye
title_full Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye
title_fullStr Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye
title_full_unstemmed Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye
title_sort Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye
dc.creator.none.fl_str_mv Spitzmaul, Guillermo Federico
German, Olga Lorena
Jentsch, Thomas
author Spitzmaul, Guillermo Federico
author_facet Spitzmaul, Guillermo Federico
German, Olga Lorena
Jentsch, Thomas
author_role author
author2 German, Olga Lorena
Jentsch, Thomas
author2_role author
author
dc.subject.none.fl_str_mv RETINA
KCNQ
POTASSIUM
CHANNELS
topic RETINA
KCNQ
POTASSIUM
CHANNELS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Neuronal potassium channels KCNQ (Kv7) are important for neuron physiology because they regulate their excitability. Four of the five KCNQ channel genes are mutated in human genetic diseases. There are transgenic mice for KCNQ3, -4 and -5 channels, which we used in previous works, allowing the study of protein function and mutation-associated pathologies. Recent publications showed the expression of KCNQ2 to -5 in primate eye and also a possible link between Kcnq5 gen and refractive errors. For these reasons, we investigate the expression and function of these channels in wild-type, Kcnq4-/- and Kcnq5dn/dn mice. We found a weak labeling of KCNQ4 in retinal pigmented epithelium cells, which is enhanced in pigmented cells of ciliary body. KCNQ3 was found only in cells of the non-pigmented epitehlium of the ciliary body. Opposite to what was reported in other species, no KCNQ channel subunits were found in mouse retina. Besides, immature mouse retinal neurons in culture did not show M-like potassium currents, which are generated by KCNQ channels in neurons. Our results suggest that KCNQ channels may participate in the formation of aquous humor of the eye, providing part of the companion positive current during chloride transport trough the epithelium.
Fil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Jentsch, Thomas. Leibniz-Institut fuer Molekulare Pharmakologie; Alemania. MaxDelbrueck-Centrum fuer Molekulare Medizin; Alemania
XXIX Annual Meeting of Sociedad Argentina de Investigación en Neurociencias
Huerta Grande
Argentina
Sociedad Argentina e Investigación en Neurociencias
description Neuronal potassium channels KCNQ (Kv7) are important for neuron physiology because they regulate their excitability. Four of the five KCNQ channel genes are mutated in human genetic diseases. There are transgenic mice for KCNQ3, -4 and -5 channels, which we used in previous works, allowing the study of protein function and mutation-associated pathologies. Recent publications showed the expression of KCNQ2 to -5 in primate eye and also a possible link between Kcnq5 gen and refractive errors. For these reasons, we investigate the expression and function of these channels in wild-type, Kcnq4-/- and Kcnq5dn/dn mice. We found a weak labeling of KCNQ4 in retinal pigmented epithelium cells, which is enhanced in pigmented cells of ciliary body. KCNQ3 was found only in cells of the non-pigmented epitehlium of the ciliary body. Opposite to what was reported in other species, no KCNQ channel subunits were found in mouse retina. Besides, immature mouse retinal neurons in culture did not show M-like potassium currents, which are generated by KCNQ channels in neurons. Our results suggest that KCNQ channels may participate in the formation of aquous humor of the eye, providing part of the companion positive current during chloride transport trough the epithelium.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222187
Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye; XXIX Annual Meeting of Sociedad Argentina de Investigación en Neurociencias; Huerta Grande; Argentina; 2014; 97-97
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222187
identifier_str_mv Analysis of expression and function of voltage-activated potassium KCNQ channels on mouse eye; XXIX Annual Meeting of Sociedad Argentina de Investigación en Neurociencias; Huerta Grande; Argentina; 2014; 97-97
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/congresos-san-2/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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