Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit

Autores
Bayasgalan, T.; Stupniki, Sofia; Kovács, A.; Csemer, A.; Szentesi, P.; Pocsai, K.; Dionisio, Leonardo Raul; Spitzmaul, Guillermo Federico; Pal, B.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The pedunculopontine nucleus (PPN), a structure known as a cholinergic member of the reticular activating system (RAS), is source and target of cholinergic neuromodulation and contributes to the regulation of the sleep-wakefulness cycle. The M-current is a voltage-gated potassium current modulated mainly by cholinergic signaling. KCNQ subunits ensemble into ion channels responsible for the M-current. In the central nervous system, KCNQ4 expression is restricted to certain brainstem structures such as the RAS nuclei. Here, we investigated the presence and functional significance of KCNQ4 in the PPN by behavioral studies and the gene and protein expressions and slice electrophysiology using a mouse model lacking KCNQ4 expression. We found that this mouse has alterations in the adaptation to changes in light-darkness cycles, representing the potential role of KCNQ4 in the regulation of the sleep-wakefulness cycle. As cholinergic neurons from the PPN participate in the regulation of this cycle, we investigated whether the cholinergic PPN might also possess functional KCNQ4 subunits. Although the M-current is an electrophysiological hallmark of cholinergic neurons, only a subpopulation of them had KCNQ4-dependent M-current. Interestingly, the absence of the KCNQ4 subunit altered the expression patterns of the other KCNQ subunits in the PPN. We also determined that, in wild-type animals, the cholinergic inputs of the PPN modulated the M-current, and these in turn can modulate the level of synchronization between neighboring PPN neurons. Taken together, the KCNQ4 subunit is present in a subpopulation of PPN cholinergic neurons, and it may contribute to the regulation of the sleep-wakefulness cycle.
Fil: Bayasgalan, T.. University of Debrecen; Hungría
Fil: Stupniki, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Kovács, A.. University of Debrecen; Hungría
Fil: Csemer, A.. University of Debrecen; Hungría
Fil: Szentesi, P.. University of Debrecen; Hungría
Fil: Pocsai, K.. University of Debrecen; Hungría
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pal, B.. University of Debrecen; Hungría
Materia
KCNQ4 (KV7.4)
M-CURRENT
NEURONAL SYNCHRONIZATION
NON-SYNDROMIC HEARING LOSS (DFNA2)
PEDUNCULOPONTINE NUCLEUS
POTASSIUM CHANNELS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151662
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/151662
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunitBayasgalan, T.Stupniki, SofiaKovács, A.Csemer, A.Szentesi, P.Pocsai, K.Dionisio, Leonardo RaulSpitzmaul, Guillermo FedericoPal, B.KCNQ4 (KV7.4)M-CURRENTNEURONAL SYNCHRONIZATIONNON-SYNDROMIC HEARING LOSS (DFNA2)PEDUNCULOPONTINE NUCLEUSPOTASSIUM CHANNELShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The pedunculopontine nucleus (PPN), a structure known as a cholinergic member of the reticular activating system (RAS), is source and target of cholinergic neuromodulation and contributes to the regulation of the sleep-wakefulness cycle. The M-current is a voltage-gated potassium current modulated mainly by cholinergic signaling. KCNQ subunits ensemble into ion channels responsible for the M-current. In the central nervous system, KCNQ4 expression is restricted to certain brainstem structures such as the RAS nuclei. Here, we investigated the presence and functional significance of KCNQ4 in the PPN by behavioral studies and the gene and protein expressions and slice electrophysiology using a mouse model lacking KCNQ4 expression. We found that this mouse has alterations in the adaptation to changes in light-darkness cycles, representing the potential role of KCNQ4 in the regulation of the sleep-wakefulness cycle. As cholinergic neurons from the PPN participate in the regulation of this cycle, we investigated whether the cholinergic PPN might also possess functional KCNQ4 subunits. Although the M-current is an electrophysiological hallmark of cholinergic neurons, only a subpopulation of them had KCNQ4-dependent M-current. Interestingly, the absence of the KCNQ4 subunit altered the expression patterns of the other KCNQ subunits in the PPN. We also determined that, in wild-type animals, the cholinergic inputs of the PPN modulated the M-current, and these in turn can modulate the level of synchronization between neighboring PPN neurons. Taken together, the KCNQ4 subunit is present in a subpopulation of PPN cholinergic neurons, and it may contribute to the regulation of the sleep-wakefulness cycle.Fil: Bayasgalan, T.. University of Debrecen; HungríaFil: Stupniki, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Kovács, A.. University of Debrecen; HungríaFil: Csemer, A.. University of Debrecen; HungríaFil: Szentesi, P.. University of Debrecen; HungríaFil: Pocsai, K.. University of Debrecen; HungríaFil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Pal, B.. University of Debrecen; HungríaFrontiers Media S.A.2021-07-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151662Bayasgalan, T.; Stupniki, Sofia; Kovács, A.; Csemer, A.; Szentesi, P.; et al.; Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit; Frontiers Media S.A.; Frontiers in Cellular Neuroscience; 15; 707789; 26-7-2021; 1-161662-5102CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fncel.2021.707789/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2021.707789info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:09Zoai:ri.conicet.gov.ar:11336/151662instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:09.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit
title Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit
spellingShingle Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit
Bayasgalan, T.
KCNQ4 (KV7.4)
M-CURRENT
NEURONAL SYNCHRONIZATION
NON-SYNDROMIC HEARING LOSS (DFNA2)
PEDUNCULOPONTINE NUCLEUS
POTASSIUM CHANNELS
title_short Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit
title_full Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit
title_fullStr Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit
title_full_unstemmed Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit
title_sort Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit
dc.creator.none.fl_str_mv Bayasgalan, T.
Stupniki, Sofia
Kovács, A.
Csemer, A.
Szentesi, P.
Pocsai, K.
Dionisio, Leonardo Raul
Spitzmaul, Guillermo Federico
Pal, B.
author Bayasgalan, T.
author_facet Bayasgalan, T.
Stupniki, Sofia
Kovács, A.
Csemer, A.
Szentesi, P.
Pocsai, K.
Dionisio, Leonardo Raul
Spitzmaul, Guillermo Federico
Pal, B.
author_role author
author2 Stupniki, Sofia
Kovács, A.
Csemer, A.
Szentesi, P.
Pocsai, K.
Dionisio, Leonardo Raul
Spitzmaul, Guillermo Federico
Pal, B.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv KCNQ4 (KV7.4)
M-CURRENT
NEURONAL SYNCHRONIZATION
NON-SYNDROMIC HEARING LOSS (DFNA2)
PEDUNCULOPONTINE NUCLEUS
POTASSIUM CHANNELS
topic KCNQ4 (KV7.4)
M-CURRENT
NEURONAL SYNCHRONIZATION
NON-SYNDROMIC HEARING LOSS (DFNA2)
PEDUNCULOPONTINE NUCLEUS
POTASSIUM CHANNELS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The pedunculopontine nucleus (PPN), a structure known as a cholinergic member of the reticular activating system (RAS), is source and target of cholinergic neuromodulation and contributes to the regulation of the sleep-wakefulness cycle. The M-current is a voltage-gated potassium current modulated mainly by cholinergic signaling. KCNQ subunits ensemble into ion channels responsible for the M-current. In the central nervous system, KCNQ4 expression is restricted to certain brainstem structures such as the RAS nuclei. Here, we investigated the presence and functional significance of KCNQ4 in the PPN by behavioral studies and the gene and protein expressions and slice electrophysiology using a mouse model lacking KCNQ4 expression. We found that this mouse has alterations in the adaptation to changes in light-darkness cycles, representing the potential role of KCNQ4 in the regulation of the sleep-wakefulness cycle. As cholinergic neurons from the PPN participate in the regulation of this cycle, we investigated whether the cholinergic PPN might also possess functional KCNQ4 subunits. Although the M-current is an electrophysiological hallmark of cholinergic neurons, only a subpopulation of them had KCNQ4-dependent M-current. Interestingly, the absence of the KCNQ4 subunit altered the expression patterns of the other KCNQ subunits in the PPN. We also determined that, in wild-type animals, the cholinergic inputs of the PPN modulated the M-current, and these in turn can modulate the level of synchronization between neighboring PPN neurons. Taken together, the KCNQ4 subunit is present in a subpopulation of PPN cholinergic neurons, and it may contribute to the regulation of the sleep-wakefulness cycle.
Fil: Bayasgalan, T.. University of Debrecen; Hungría
Fil: Stupniki, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Kovács, A.. University of Debrecen; Hungría
Fil: Csemer, A.. University of Debrecen; Hungría
Fil: Szentesi, P.. University of Debrecen; Hungría
Fil: Pocsai, K.. University of Debrecen; Hungría
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pal, B.. University of Debrecen; Hungría
description The pedunculopontine nucleus (PPN), a structure known as a cholinergic member of the reticular activating system (RAS), is source and target of cholinergic neuromodulation and contributes to the regulation of the sleep-wakefulness cycle. The M-current is a voltage-gated potassium current modulated mainly by cholinergic signaling. KCNQ subunits ensemble into ion channels responsible for the M-current. In the central nervous system, KCNQ4 expression is restricted to certain brainstem structures such as the RAS nuclei. Here, we investigated the presence and functional significance of KCNQ4 in the PPN by behavioral studies and the gene and protein expressions and slice electrophysiology using a mouse model lacking KCNQ4 expression. We found that this mouse has alterations in the adaptation to changes in light-darkness cycles, representing the potential role of KCNQ4 in the regulation of the sleep-wakefulness cycle. As cholinergic neurons from the PPN participate in the regulation of this cycle, we investigated whether the cholinergic PPN might also possess functional KCNQ4 subunits. Although the M-current is an electrophysiological hallmark of cholinergic neurons, only a subpopulation of them had KCNQ4-dependent M-current. Interestingly, the absence of the KCNQ4 subunit altered the expression patterns of the other KCNQ subunits in the PPN. We also determined that, in wild-type animals, the cholinergic inputs of the PPN modulated the M-current, and these in turn can modulate the level of synchronization between neighboring PPN neurons. Taken together, the KCNQ4 subunit is present in a subpopulation of PPN cholinergic neurons, and it may contribute to the regulation of the sleep-wakefulness cycle.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151662
Bayasgalan, T.; Stupniki, Sofia; Kovács, A.; Csemer, A.; Szentesi, P.; et al.; Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit; Frontiers Media S.A.; Frontiers in Cellular Neuroscience; 15; 707789; 26-7-2021; 1-16
1662-5102
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151662
identifier_str_mv Bayasgalan, T.; Stupniki, Sofia; Kovács, A.; Csemer, A.; Szentesi, P.; et al.; Alteration of mesopontine cholinergic function by the lack of KCNQ4 subunit; Frontiers Media S.A.; Frontiers in Cellular Neuroscience; 15; 707789; 26-7-2021; 1-16
1662-5102
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fncel.2021.707789/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2021.707789
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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