Expression and function of KCNQ channels in Ciliary Body

Autores
Barila, Esteban Pablo; German, Olga Lorena; Carignano, Camila; Spitzmaul, Guillermo Federico
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The ciliary epithelium (CE) consists of two layers of secretory cells which are responsible for aqueous humor formation. One strategy in the treatment of glaucoma is to reduce the inflow of aqueous humour, which relays on Cl- movement trough the epithelia. The rate of anion secretion depends on pumps, transporters, Na+ channels and still-unknown K+ channels, which aid the outflow of Cl- by increasing its driving force. KCNQ channels (Kv7) are voltagegated K+ channels with 5 members in mammals (KCNQ1-5). Among some of their functions they participate in cell volume regulation and epithelial transport. We study the role of KCNQ channels in this process using KO mice for each channel. Whole-eye RT-PCR analysis showed expression of KCNQ3, 4 and 5. Total homogenates of CE exhibited immunoreactive bands for KCNQ4. Using KO controlled immunohistochemistry we found specific labeling for KCNQ4 on the membrane of the pigmented cells (PC) of the CE, while KCNQ3 and 5 were not present. KCNQ4 was confined to the basolateral as well as the apical membrane of PCs, co-localizing with Connexin-43. Preliminary patch-clamp studies of non-pigmented cells lacking KCNQ4 expression, showed no changes in potassium currents. We conclude that KCNQ4 is expressed in the pigmented cells of the CE. This channel could contribute to the Cl- movement from the ciliary body stroma to the aqueous humor, being responsible for the K+ current.
Fil: Barila, Esteban Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Carignano, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
XXXII Congreso Anual Sociedad Argentina de Investigación en Neurociencias
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
EYE
POTASSIUM
CHANNEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/235368
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Expression and function of KCNQ channels in Ciliary BodyBarila, Esteban PabloGerman, Olga LorenaCarignano, CamilaSpitzmaul, Guillermo FedericoEYEPOTASSIUMCHANNELhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The ciliary epithelium (CE) consists of two layers of secretory cells which are responsible for aqueous humor formation. One strategy in the treatment of glaucoma is to reduce the inflow of aqueous humour, which relays on Cl- movement trough the epithelia. The rate of anion secretion depends on pumps, transporters, Na+ channels and still-unknown K+ channels, which aid the outflow of Cl- by increasing its driving force. KCNQ channels (Kv7) are voltagegated K+ channels with 5 members in mammals (KCNQ1-5). Among some of their functions they participate in cell volume regulation and epithelial transport. We study the role of KCNQ channels in this process using KO mice for each channel. Whole-eye RT-PCR analysis showed expression of KCNQ3, 4 and 5. Total homogenates of CE exhibited immunoreactive bands for KCNQ4. Using KO controlled immunohistochemistry we found specific labeling for KCNQ4 on the membrane of the pigmented cells (PC) of the CE, while KCNQ3 and 5 were not present. KCNQ4 was confined to the basolateral as well as the apical membrane of PCs, co-localizing with Connexin-43. Preliminary patch-clamp studies of non-pigmented cells lacking KCNQ4 expression, showed no changes in potassium currents. We conclude that KCNQ4 is expressed in the pigmented cells of the CE. This channel could contribute to the Cl- movement from the ciliary body stroma to the aqueous humor, being responsible for the K+ current.Fil: Barila, Esteban Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Carignano, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaXXXII Congreso Anual Sociedad Argentina de Investigación en NeurocienciasMar del PlataArgentinaSociedad Argentina de Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencias2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/235368Expression and function of KCNQ channels in Ciliary Body; XXXII Congreso Anual Sociedad Argentina de Investigación en Neurociencias; Mar del Plata; Argentina; 2017; 270-270CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2017_Mar-del-Plata_-libro-de-resumenes.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:10Zoai:ri.conicet.gov.ar:11336/235368instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:10.757CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression and function of KCNQ channels in Ciliary Body
title Expression and function of KCNQ channels in Ciliary Body
spellingShingle Expression and function of KCNQ channels in Ciliary Body
Barila, Esteban Pablo
EYE
POTASSIUM
CHANNEL
title_short Expression and function of KCNQ channels in Ciliary Body
title_full Expression and function of KCNQ channels in Ciliary Body
title_fullStr Expression and function of KCNQ channels in Ciliary Body
title_full_unstemmed Expression and function of KCNQ channels in Ciliary Body
title_sort Expression and function of KCNQ channels in Ciliary Body
dc.creator.none.fl_str_mv Barila, Esteban Pablo
German, Olga Lorena
Carignano, Camila
Spitzmaul, Guillermo Federico
author Barila, Esteban Pablo
author_facet Barila, Esteban Pablo
German, Olga Lorena
Carignano, Camila
Spitzmaul, Guillermo Federico
author_role author
author2 German, Olga Lorena
Carignano, Camila
Spitzmaul, Guillermo Federico
author2_role author
author
author
dc.subject.none.fl_str_mv EYE
POTASSIUM
CHANNEL
topic EYE
POTASSIUM
CHANNEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The ciliary epithelium (CE) consists of two layers of secretory cells which are responsible for aqueous humor formation. One strategy in the treatment of glaucoma is to reduce the inflow of aqueous humour, which relays on Cl- movement trough the epithelia. The rate of anion secretion depends on pumps, transporters, Na+ channels and still-unknown K+ channels, which aid the outflow of Cl- by increasing its driving force. KCNQ channels (Kv7) are voltagegated K+ channels with 5 members in mammals (KCNQ1-5). Among some of their functions they participate in cell volume regulation and epithelial transport. We study the role of KCNQ channels in this process using KO mice for each channel. Whole-eye RT-PCR analysis showed expression of KCNQ3, 4 and 5. Total homogenates of CE exhibited immunoreactive bands for KCNQ4. Using KO controlled immunohistochemistry we found specific labeling for KCNQ4 on the membrane of the pigmented cells (PC) of the CE, while KCNQ3 and 5 were not present. KCNQ4 was confined to the basolateral as well as the apical membrane of PCs, co-localizing with Connexin-43. Preliminary patch-clamp studies of non-pigmented cells lacking KCNQ4 expression, showed no changes in potassium currents. We conclude that KCNQ4 is expressed in the pigmented cells of the CE. This channel could contribute to the Cl- movement from the ciliary body stroma to the aqueous humor, being responsible for the K+ current.
Fil: Barila, Esteban Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Carignano, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
XXXII Congreso Anual Sociedad Argentina de Investigación en Neurociencias
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Neurociencias
description The ciliary epithelium (CE) consists of two layers of secretory cells which are responsible for aqueous humor formation. One strategy in the treatment of glaucoma is to reduce the inflow of aqueous humour, which relays on Cl- movement trough the epithelia. The rate of anion secretion depends on pumps, transporters, Na+ channels and still-unknown K+ channels, which aid the outflow of Cl- by increasing its driving force. KCNQ channels (Kv7) are voltagegated K+ channels with 5 members in mammals (KCNQ1-5). Among some of their functions they participate in cell volume regulation and epithelial transport. We study the role of KCNQ channels in this process using KO mice for each channel. Whole-eye RT-PCR analysis showed expression of KCNQ3, 4 and 5. Total homogenates of CE exhibited immunoreactive bands for KCNQ4. Using KO controlled immunohistochemistry we found specific labeling for KCNQ4 on the membrane of the pigmented cells (PC) of the CE, while KCNQ3 and 5 were not present. KCNQ4 was confined to the basolateral as well as the apical membrane of PCs, co-localizing with Connexin-43. Preliminary patch-clamp studies of non-pigmented cells lacking KCNQ4 expression, showed no changes in potassium currents. We conclude that KCNQ4 is expressed in the pigmented cells of the CE. This channel could contribute to the Cl- movement from the ciliary body stroma to the aqueous humor, being responsible for the K+ current.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/235368
Expression and function of KCNQ channels in Ciliary Body; XXXII Congreso Anual Sociedad Argentina de Investigación en Neurociencias; Mar del Plata; Argentina; 2017; 270-270
CONICET Digital
CONICET
url http://hdl.handle.net/11336/235368
identifier_str_mv Expression and function of KCNQ channels in Ciliary Body; XXXII Congreso Anual Sociedad Argentina de Investigación en Neurociencias; Mar del Plata; Argentina; 2017; 270-270
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2017_Mar-del-Plata_-libro-de-resumenes.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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