Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity

Autores
Bieczynski, Flavia; Burkhardt Medicke, Kathleen; Luquet, Carlos Marcelo; Scholz, Stefan; Luckenbach, Till
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
ATP‐binding cassette (ABC) transporter proteins include efflux pumps that confer multixenobiotic resistance to zebrafish embryos, a valuable toxico/pharmacological model. Here, we established an automated microscopy‐based rhodamine B dye accumulation assay in which enhanced dye accumulation in live zebrafish embryos indicates inhibition of multixenobiotic efflux transporter activity. Twenty structurally divergent known substrates and/or inhibitors of human ABC transporters and environmentally relevant compounds were examined using this assay and the ATPase activity of recombinant zebrafish Abcb4 as readouts. These two assays confirmed that Abcb4 functions as an efflux transporter in zebrafish, whereas they gave discordant results for some of the tested substances. The dye accumulation assay in zebrafish embryos could be useful to screen environmental pollutants and other chemicals for efflux transporter interaction in a medium‐throughput fashion.
Fil: Bieczynski, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue | Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue; Argentina
Fil: Burkhardt Medicke, Kathleen. Universitat Leipzig; Alemania. Helmholtz Centre for Environmental Research; Alemania
Fil: Luquet, Carlos Marcelo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Patagonia Norte. Instituto de Investigaciones En Biodiversidad y Medioambiente. Subsede Junín de Los Andes-inibioma-centro de Ecología Aplicada del Neuquén (cean) | Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto de Investigaciones En Biodiversidad y Medioambiente. Subsede Junín de Los Andes-inibioma-centro de Ecología Aplicada del Neuquén (cean).; Argentina
Fil: Scholz, Stefan. Universitat Leipzig; Alemania. Helmholtz Centre for Environmental Research; Alemania
Fil: Luckenbach, Till. Universitat Leipzig; Alemania. Helmholtz Centre for Environmental Research; Alemania
Materia
ABC EFFLUX TRANSPORTER
ATPASE ACTIVITY
CHEMOSENSITIZER
DYE ACCUMULATION ASSAY
ZEBRAFISH ABCB4
ZEBRAFISH EMBRYO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/162489
Acceder
id CONICETDig_e26e3b3235cf75e786d0e8684ead6f30
oai_identifier_str oai:ri.conicet.gov.ar:11336/162489
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activityBieczynski, FlaviaBurkhardt Medicke, KathleenLuquet, Carlos MarceloScholz, StefanLuckenbach, TillABC EFFLUX TRANSPORTERATPASE ACTIVITYCHEMOSENSITIZERDYE ACCUMULATION ASSAYZEBRAFISH ABCB4ZEBRAFISH EMBRYOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1ATP‐binding cassette (ABC) transporter proteins include efflux pumps that confer multixenobiotic resistance to zebrafish embryos, a valuable toxico/pharmacological model. Here, we established an automated microscopy‐based rhodamine B dye accumulation assay in which enhanced dye accumulation in live zebrafish embryos indicates inhibition of multixenobiotic efflux transporter activity. Twenty structurally divergent known substrates and/or inhibitors of human ABC transporters and environmentally relevant compounds were examined using this assay and the ATPase activity of recombinant zebrafish Abcb4 as readouts. These two assays confirmed that Abcb4 functions as an efflux transporter in zebrafish, whereas they gave discordant results for some of the tested substances. The dye accumulation assay in zebrafish embryos could be useful to screen environmental pollutants and other chemicals for efflux transporter interaction in a medium‐throughput fashion.Fil: Bieczynski, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue | Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue; ArgentinaFil: Burkhardt Medicke, Kathleen. Universitat Leipzig; Alemania. Helmholtz Centre for Environmental Research; AlemaniaFil: Luquet, Carlos Marcelo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Patagonia Norte. Instituto de Investigaciones En Biodiversidad y Medioambiente. Subsede Junín de Los Andes-inibioma-centro de Ecología Aplicada del Neuquén (cean) | Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto de Investigaciones En Biodiversidad y Medioambiente. Subsede Junín de Los Andes-inibioma-centro de Ecología Aplicada del Neuquén (cean).; ArgentinaFil: Scholz, Stefan. Universitat Leipzig; Alemania. Helmholtz Centre for Environmental Research; AlemaniaFil: Luckenbach, Till. Universitat Leipzig; Alemania. Helmholtz Centre for Environmental Research; AlemaniaElsevier Science2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/162489Bieczynski, Flavia; Burkhardt Medicke, Kathleen; Luquet, Carlos Marcelo; Scholz, Stefan; Luckenbach, Till; Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity; Elsevier Science; FEBS Letters; 595; 6; 12-2020; 828-8430014-5793CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/1873-3468.14015info:eu-repo/semantics/altIdentifier/doi/10.1002/1873-3468.14015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:01Zoai:ri.conicet.gov.ar:11336/162489instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:01.364CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
title Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
spellingShingle Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
Bieczynski, Flavia
ABC EFFLUX TRANSPORTER
ATPASE ACTIVITY
CHEMOSENSITIZER
DYE ACCUMULATION ASSAY
ZEBRAFISH ABCB4
ZEBRAFISH EMBRYO
title_short Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
title_full Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
title_fullStr Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
title_full_unstemmed Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
title_sort Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
dc.creator.none.fl_str_mv Bieczynski, Flavia
Burkhardt Medicke, Kathleen
Luquet, Carlos Marcelo
Scholz, Stefan
Luckenbach, Till
author Bieczynski, Flavia
author_facet Bieczynski, Flavia
Burkhardt Medicke, Kathleen
Luquet, Carlos Marcelo
Scholz, Stefan
Luckenbach, Till
author_role author
author2 Burkhardt Medicke, Kathleen
Luquet, Carlos Marcelo
Scholz, Stefan
Luckenbach, Till
author2_role author
author
author
author
dc.subject.none.fl_str_mv ABC EFFLUX TRANSPORTER
ATPASE ACTIVITY
CHEMOSENSITIZER
DYE ACCUMULATION ASSAY
ZEBRAFISH ABCB4
ZEBRAFISH EMBRYO
topic ABC EFFLUX TRANSPORTER
ATPASE ACTIVITY
CHEMOSENSITIZER
DYE ACCUMULATION ASSAY
ZEBRAFISH ABCB4
ZEBRAFISH EMBRYO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv ATP‐binding cassette (ABC) transporter proteins include efflux pumps that confer multixenobiotic resistance to zebrafish embryos, a valuable toxico/pharmacological model. Here, we established an automated microscopy‐based rhodamine B dye accumulation assay in which enhanced dye accumulation in live zebrafish embryos indicates inhibition of multixenobiotic efflux transporter activity. Twenty structurally divergent known substrates and/or inhibitors of human ABC transporters and environmentally relevant compounds were examined using this assay and the ATPase activity of recombinant zebrafish Abcb4 as readouts. These two assays confirmed that Abcb4 functions as an efflux transporter in zebrafish, whereas they gave discordant results for some of the tested substances. The dye accumulation assay in zebrafish embryos could be useful to screen environmental pollutants and other chemicals for efflux transporter interaction in a medium‐throughput fashion.
Fil: Bieczynski, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue | Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue. Instituto de Biotecnología Agropecuaria del Comahue; Argentina
Fil: Burkhardt Medicke, Kathleen. Universitat Leipzig; Alemania. Helmholtz Centre for Environmental Research; Alemania
Fil: Luquet, Carlos Marcelo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Patagonia Norte. Instituto de Investigaciones En Biodiversidad y Medioambiente. Subsede Junín de Los Andes-inibioma-centro de Ecología Aplicada del Neuquén (cean) | Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto de Investigaciones En Biodiversidad y Medioambiente. Subsede Junín de Los Andes-inibioma-centro de Ecología Aplicada del Neuquén (cean).; Argentina
Fil: Scholz, Stefan. Universitat Leipzig; Alemania. Helmholtz Centre for Environmental Research; Alemania
Fil: Luckenbach, Till. Universitat Leipzig; Alemania. Helmholtz Centre for Environmental Research; Alemania
description ATP‐binding cassette (ABC) transporter proteins include efflux pumps that confer multixenobiotic resistance to zebrafish embryos, a valuable toxico/pharmacological model. Here, we established an automated microscopy‐based rhodamine B dye accumulation assay in which enhanced dye accumulation in live zebrafish embryos indicates inhibition of multixenobiotic efflux transporter activity. Twenty structurally divergent known substrates and/or inhibitors of human ABC transporters and environmentally relevant compounds were examined using this assay and the ATPase activity of recombinant zebrafish Abcb4 as readouts. These two assays confirmed that Abcb4 functions as an efflux transporter in zebrafish, whereas they gave discordant results for some of the tested substances. The dye accumulation assay in zebrafish embryos could be useful to screen environmental pollutants and other chemicals for efflux transporter interaction in a medium‐throughput fashion.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/162489
Bieczynski, Flavia; Burkhardt Medicke, Kathleen; Luquet, Carlos Marcelo; Scholz, Stefan; Luckenbach, Till; Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity; Elsevier Science; FEBS Letters; 595; 6; 12-2020; 828-843
0014-5793
CONICET Digital
CONICET
url http://hdl.handle.net/11336/162489
identifier_str_mv Bieczynski, Flavia; Burkhardt Medicke, Kathleen; Luquet, Carlos Marcelo; Scholz, Stefan; Luckenbach, Till; Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity; Elsevier Science; FEBS Letters; 595; 6; 12-2020; 828-843
0014-5793
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/1873-3468.14015
info:eu-repo/semantics/altIdentifier/doi/10.1002/1873-3468.14015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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