Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)

Autores
Garbinato, Cristiane; Alves Lima Rezende, Cássia; Schneider, Sabrina Ester; Pedroso, Jefferson; dos Santos, Aline E.; Petry, Fernanda; Aguiar, Gean Pablo S.; Girardi Müller, Liz; Lanza, Marcelo; Piato, Angelo; Vladimir Oliveira, J.; Siebel, Anna Maria
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Epilepsy affects around 50 million people worldwide, and an important number of patients (30%) fail to respond to any available antiepileptic drug. Previous studies have shown that luteolin presents a promising potential as an anticonvulsant. On the other hand, different studies showed that luteolin does not promote anticonvulsant effects. Therefore, there is a lack of consensus about the use of luteolin for seizure control. Luteolin low bioavailability could be a limiting factor to obtain better results. Attractively, micronization technology has been applied to improve flavonoids bioavailability. Thus, the present study aimed to investigate the effects of luteolin on its raw form and micronized luteolin in a PTZ-induced seizure model in adult zebrafish (Danio rerio). Our results demonstrate that luteolin and micronized luteolin did not block PTZ-induced seizures in adult zebrafish. Also, luteolin and micronized luteolin did not provoke behavioral changes. Finally, our results show that 24 h after seizure occurrence, no changes were detected for p70S6Kb, interleukin 1β, and caspase-3 transcript levels. Altogether, we failed to observe an anticonvulsant potential of luteolin in adult zebrafish, even in its micronized form. However, we recommend new studies to investigate luteolin benefits in epilepsy.
Fil: Garbinato, Cristiane. No especifíca;
Fil: Alves Lima Rezende, Cássia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Schneider, Sabrina Ester. No especifíca;
Fil: Pedroso, Jefferson. No especifíca;
Fil: dos Santos, Aline E.. Universidade Federal de Santa Catarina; Brasil
Fil: Petry, Fernanda. No especifíca;
Fil: Aguiar, Gean Pablo S.. No especifíca;
Fil: Girardi Müller, Liz. No especifíca;
Fil: Lanza, Marcelo. Universidade Federal de Santa Catarina; Brasil
Fil: Piato, Angelo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Vladimir Oliveira, J.. Universidade Federal de Santa Catarina; Brasil
Fil: Siebel, Anna Maria. No especifíca;
Materia
EPILEPSY
LUTEOLIN
MICRONIZATION
SEIZURE
ZEBRAFISH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212448
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212448
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)Garbinato, CristianeAlves Lima Rezende, CássiaSchneider, Sabrina EsterPedroso, Jeffersondos Santos, Aline E.Petry, FernandaAguiar, Gean Pablo S.Girardi Müller, LizLanza, MarceloPiato, AngeloVladimir Oliveira, J.Siebel, Anna MariaEPILEPSYLUTEOLINMICRONIZATIONSEIZUREZEBRAFISHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Epilepsy affects around 50 million people worldwide, and an important number of patients (30%) fail to respond to any available antiepileptic drug. Previous studies have shown that luteolin presents a promising potential as an anticonvulsant. On the other hand, different studies showed that luteolin does not promote anticonvulsant effects. Therefore, there is a lack of consensus about the use of luteolin for seizure control. Luteolin low bioavailability could be a limiting factor to obtain better results. Attractively, micronization technology has been applied to improve flavonoids bioavailability. Thus, the present study aimed to investigate the effects of luteolin on its raw form and micronized luteolin in a PTZ-induced seizure model in adult zebrafish (Danio rerio). Our results demonstrate that luteolin and micronized luteolin did not block PTZ-induced seizures in adult zebrafish. Also, luteolin and micronized luteolin did not provoke behavioral changes. Finally, our results show that 24 h after seizure occurrence, no changes were detected for p70S6Kb, interleukin 1β, and caspase-3 transcript levels. Altogether, we failed to observe an anticonvulsant potential of luteolin in adult zebrafish, even in its micronized form. However, we recommend new studies to investigate luteolin benefits in epilepsy.Fil: Garbinato, Cristiane. No especifíca;Fil: Alves Lima Rezende, Cássia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Schneider, Sabrina Ester. No especifíca;Fil: Pedroso, Jefferson. No especifíca;Fil: dos Santos, Aline E.. Universidade Federal de Santa Catarina; BrasilFil: Petry, Fernanda. No especifíca;Fil: Aguiar, Gean Pablo S.. No especifíca;Fil: Girardi Müller, Liz. No especifíca;Fil: Lanza, Marcelo. Universidade Federal de Santa Catarina; BrasilFil: Piato, Angelo. Universidade Federal do Rio Grande do Sul; BrasilFil: Vladimir Oliveira, J.. Universidade Federal de Santa Catarina; BrasilFil: Siebel, Anna Maria. No especifíca;Springer/Plenum Publishers2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212448Garbinato, Cristiane; Alves Lima Rezende, Cássia; Schneider, Sabrina Ester; Pedroso, Jefferson; dos Santos, Aline E.; et al.; Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio); Springer/Plenum Publishers; Neurochemical Research; 46; 11; 11-2021; 3025-30340364-3190CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11064-021-03409-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:20Zoai:ri.conicet.gov.ar:11336/212448instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:20.984CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)
title Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)
spellingShingle Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)
Garbinato, Cristiane
EPILEPSY
LUTEOLIN
MICRONIZATION
SEIZURE
ZEBRAFISH
title_short Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)
title_full Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)
title_fullStr Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)
title_full_unstemmed Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)
title_sort Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)
dc.creator.none.fl_str_mv Garbinato, Cristiane
Alves Lima Rezende, Cássia
Schneider, Sabrina Ester
Pedroso, Jefferson
dos Santos, Aline E.
Petry, Fernanda
Aguiar, Gean Pablo S.
Girardi Müller, Liz
Lanza, Marcelo
Piato, Angelo
Vladimir Oliveira, J.
Siebel, Anna Maria
author Garbinato, Cristiane
author_facet Garbinato, Cristiane
Alves Lima Rezende, Cássia
Schneider, Sabrina Ester
Pedroso, Jefferson
dos Santos, Aline E.
Petry, Fernanda
Aguiar, Gean Pablo S.
Girardi Müller, Liz
Lanza, Marcelo
Piato, Angelo
Vladimir Oliveira, J.
Siebel, Anna Maria
author_role author
author2 Alves Lima Rezende, Cássia
Schneider, Sabrina Ester
Pedroso, Jefferson
dos Santos, Aline E.
Petry, Fernanda
Aguiar, Gean Pablo S.
Girardi Müller, Liz
Lanza, Marcelo
Piato, Angelo
Vladimir Oliveira, J.
Siebel, Anna Maria
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EPILEPSY
LUTEOLIN
MICRONIZATION
SEIZURE
ZEBRAFISH
topic EPILEPSY
LUTEOLIN
MICRONIZATION
SEIZURE
ZEBRAFISH
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Epilepsy affects around 50 million people worldwide, and an important number of patients (30%) fail to respond to any available antiepileptic drug. Previous studies have shown that luteolin presents a promising potential as an anticonvulsant. On the other hand, different studies showed that luteolin does not promote anticonvulsant effects. Therefore, there is a lack of consensus about the use of luteolin for seizure control. Luteolin low bioavailability could be a limiting factor to obtain better results. Attractively, micronization technology has been applied to improve flavonoids bioavailability. Thus, the present study aimed to investigate the effects of luteolin on its raw form and micronized luteolin in a PTZ-induced seizure model in adult zebrafish (Danio rerio). Our results demonstrate that luteolin and micronized luteolin did not block PTZ-induced seizures in adult zebrafish. Also, luteolin and micronized luteolin did not provoke behavioral changes. Finally, our results show that 24 h after seizure occurrence, no changes were detected for p70S6Kb, interleukin 1β, and caspase-3 transcript levels. Altogether, we failed to observe an anticonvulsant potential of luteolin in adult zebrafish, even in its micronized form. However, we recommend new studies to investigate luteolin benefits in epilepsy.
Fil: Garbinato, Cristiane. No especifíca;
Fil: Alves Lima Rezende, Cássia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Schneider, Sabrina Ester. No especifíca;
Fil: Pedroso, Jefferson. No especifíca;
Fil: dos Santos, Aline E.. Universidade Federal de Santa Catarina; Brasil
Fil: Petry, Fernanda. No especifíca;
Fil: Aguiar, Gean Pablo S.. No especifíca;
Fil: Girardi Müller, Liz. No especifíca;
Fil: Lanza, Marcelo. Universidade Federal de Santa Catarina; Brasil
Fil: Piato, Angelo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Vladimir Oliveira, J.. Universidade Federal de Santa Catarina; Brasil
Fil: Siebel, Anna Maria. No especifíca;
description Epilepsy affects around 50 million people worldwide, and an important number of patients (30%) fail to respond to any available antiepileptic drug. Previous studies have shown that luteolin presents a promising potential as an anticonvulsant. On the other hand, different studies showed that luteolin does not promote anticonvulsant effects. Therefore, there is a lack of consensus about the use of luteolin for seizure control. Luteolin low bioavailability could be a limiting factor to obtain better results. Attractively, micronization technology has been applied to improve flavonoids bioavailability. Thus, the present study aimed to investigate the effects of luteolin on its raw form and micronized luteolin in a PTZ-induced seizure model in adult zebrafish (Danio rerio). Our results demonstrate that luteolin and micronized luteolin did not block PTZ-induced seizures in adult zebrafish. Also, luteolin and micronized luteolin did not provoke behavioral changes. Finally, our results show that 24 h after seizure occurrence, no changes were detected for p70S6Kb, interleukin 1β, and caspase-3 transcript levels. Altogether, we failed to observe an anticonvulsant potential of luteolin in adult zebrafish, even in its micronized form. However, we recommend new studies to investigate luteolin benefits in epilepsy.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212448
Garbinato, Cristiane; Alves Lima Rezende, Cássia; Schneider, Sabrina Ester; Pedroso, Jefferson; dos Santos, Aline E.; et al.; Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio); Springer/Plenum Publishers; Neurochemical Research; 46; 11; 11-2021; 3025-3034
0364-3190
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212448
identifier_str_mv Garbinato, Cristiane; Alves Lima Rezende, Cássia; Schneider, Sabrina Ester; Pedroso, Jefferson; dos Santos, Aline E.; et al.; Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio); Springer/Plenum Publishers; Neurochemical Research; 46; 11; 11-2021; 3025-3034
0364-3190
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11064-021-03409-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer/Plenum Publishers
publisher.none.fl_str_mv Springer/Plenum Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269576605204480
score 13.13397

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