Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos

Autores
Asokan, Nandini; Mittelheisser, Vincent; Jablonski, Arnaud; Adam, Alexandre; Bizeau, Joëlle; Harlepp, Sébastien; Hyenne, Vincent; Lefebvre, Olivier; Goetz, Jacky; Tasso, Mariana Patricia; Mertz, Damien
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mesoporous silica-based nanoparticles (NPs) are promising tools for developing targeted therapeutic interventions in cancer. Endowed with a large pore silica shell suitable for drug encapsulation and with a responsive magnetic core, iron oxide stellate mesoporous silica (IO STMS) NPs stand out. Yet, their impact and potential toxicity on relevant in vivo models has not been carefully tested yet. Herein, we assessed the impact of these IO STMS nanocomposites in a syngeneic metastasis assay in zebrafish. NPs were surface-modified with human serum albumin (HSA) and loaded or not with the chemotherapeutic doxorubicin (DOX). In vitro, DOX-loaded NPs were expectedly more toxic to zebrafish melanoma (Zmel) cells than no-DOX NPs. In zebrafish embryos, the NPs were rapidly distributed through blood circulation and were found to colocalize over time with the vascular endothelium and local macrophages. Suprisingly, the NPs efficiently reduced the outgrowth of Zmel tumoral masses in an experimental metastasis assay in zebrafish embryos regardless of their loading with DOX. The anti-metastatic effect of these NPs was further improved by increasing the amount of HSA coating, also resulting in higher embryo survival. Altogether, IO STMS NPs showed promising cytotoxic effects on a relevant zebrafish metastasis model, inhibiting metastatic outgrowth in vivo independently of the drug loading. This opens the door to further testing for better exploiting their targeting and drug delivery potentialities.
Fil: Asokan, Nandini. Inserm; Francia
Fil: Mittelheisser, Vincent. Inserm; Francia
Fil: Jablonski, Arnaud. Université de Strasbourg; Francia
Fil: Adam, Alexandre. Université de Strasbourg; Francia
Fil: Bizeau, Joëlle. Université de Strasbourg; Francia
Fil: Harlepp, Sébastien. Inserm; Francia
Fil: Hyenne, Vincent. Inserm; Francia
Fil: Lefebvre, Olivier. Inserm; Francia
Fil: Goetz, Jacky. Inserm; Francia
Fil: Tasso, Mariana Patricia. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mertz, Damien. Université de Strasbourg; Francia
Materia
core-shell iron oxide-mesoporous silica
human serum albumin coating
zebrafish melanoma tumor cells
zebrafish
tumor targeting
drug delivery
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278981
Acceder
id CONICETDig_d4768311599f9ce39ac6d8aa8de103ff
oai_identifier_str oai:ri.conicet.gov.ar:11336/278981
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryosAsokan, NandiniMittelheisser, VincentJablonski, ArnaudAdam, AlexandreBizeau, JoëlleHarlepp, SébastienHyenne, VincentLefebvre, OlivierGoetz, JackyTasso, Mariana PatriciaMertz, Damiencore-shell iron oxide-mesoporous silicahuman serum albumin coatingzebrafish melanoma tumor cellszebrafishtumor targetingdrug deliveryhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Mesoporous silica-based nanoparticles (NPs) are promising tools for developing targeted therapeutic interventions in cancer. Endowed with a large pore silica shell suitable for drug encapsulation and with a responsive magnetic core, iron oxide stellate mesoporous silica (IO STMS) NPs stand out. Yet, their impact and potential toxicity on relevant in vivo models has not been carefully tested yet. Herein, we assessed the impact of these IO STMS nanocomposites in a syngeneic metastasis assay in zebrafish. NPs were surface-modified with human serum albumin (HSA) and loaded or not with the chemotherapeutic doxorubicin (DOX). In vitro, DOX-loaded NPs were expectedly more toxic to zebrafish melanoma (Zmel) cells than no-DOX NPs. In zebrafish embryos, the NPs were rapidly distributed through blood circulation and were found to colocalize over time with the vascular endothelium and local macrophages. Suprisingly, the NPs efficiently reduced the outgrowth of Zmel tumoral masses in an experimental metastasis assay in zebrafish embryos regardless of their loading with DOX. The anti-metastatic effect of these NPs was further improved by increasing the amount of HSA coating, also resulting in higher embryo survival. Altogether, IO STMS NPs showed promising cytotoxic effects on a relevant zebrafish metastasis model, inhibiting metastatic outgrowth in vivo independently of the drug loading. This opens the door to further testing for better exploiting their targeting and drug delivery potentialities.Fil: Asokan, Nandini. Inserm; FranciaFil: Mittelheisser, Vincent. Inserm; FranciaFil: Jablonski, Arnaud. Université de Strasbourg; FranciaFil: Adam, Alexandre. Université de Strasbourg; FranciaFil: Bizeau, Joëlle. Université de Strasbourg; FranciaFil: Harlepp, Sébastien. Inserm; FranciaFil: Hyenne, Vincent. Inserm; FranciaFil: Lefebvre, Olivier. Inserm; FranciaFil: Goetz, Jacky. Inserm; FranciaFil: Tasso, Mariana Patricia. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mertz, Damien. Université de Strasbourg; FranciaRoyal Society of Chemistry2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278981Asokan, Nandini; Mittelheisser, Vincent; Jablonski, Arnaud; Adam, Alexandre; Bizeau, Joëlle; et al.; Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos; Royal Society of Chemistry; Nanoscale; 17; 48; 11-2025; 27959-279742040-33642040-3372CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2025/NR/D5NR02444Ginfo:eu-repo/semantics/altIdentifier/doi/10.1039/D5NR02444Ginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:55:11Zoai:ri.conicet.gov.ar:11336/278981instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:55:12.091CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos
title Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos
spellingShingle Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos
Asokan, Nandini
core-shell iron oxide-mesoporous silica
human serum albumin coating
zebrafish melanoma tumor cells
zebrafish
tumor targeting
drug delivery
title_short Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos
title_full Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos
title_fullStr Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos
title_full_unstemmed Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos
title_sort Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos
dc.creator.none.fl_str_mv Asokan, Nandini
Mittelheisser, Vincent
Jablonski, Arnaud
Adam, Alexandre
Bizeau, Joëlle
Harlepp, Sébastien
Hyenne, Vincent
Lefebvre, Olivier
Goetz, Jacky
Tasso, Mariana Patricia
Mertz, Damien
author Asokan, Nandini
author_facet Asokan, Nandini
Mittelheisser, Vincent
Jablonski, Arnaud
Adam, Alexandre
Bizeau, Joëlle
Harlepp, Sébastien
Hyenne, Vincent
Lefebvre, Olivier
Goetz, Jacky
Tasso, Mariana Patricia
Mertz, Damien
author_role author
author2 Mittelheisser, Vincent
Jablonski, Arnaud
Adam, Alexandre
Bizeau, Joëlle
Harlepp, Sébastien
Hyenne, Vincent
Lefebvre, Olivier
Goetz, Jacky
Tasso, Mariana Patricia
Mertz, Damien
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv core-shell iron oxide-mesoporous silica
human serum albumin coating
zebrafish melanoma tumor cells
zebrafish
tumor targeting
drug delivery
topic core-shell iron oxide-mesoporous silica
human serum albumin coating
zebrafish melanoma tumor cells
zebrafish
tumor targeting
drug delivery
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Mesoporous silica-based nanoparticles (NPs) are promising tools for developing targeted therapeutic interventions in cancer. Endowed with a large pore silica shell suitable for drug encapsulation and with a responsive magnetic core, iron oxide stellate mesoporous silica (IO STMS) NPs stand out. Yet, their impact and potential toxicity on relevant in vivo models has not been carefully tested yet. Herein, we assessed the impact of these IO STMS nanocomposites in a syngeneic metastasis assay in zebrafish. NPs were surface-modified with human serum albumin (HSA) and loaded or not with the chemotherapeutic doxorubicin (DOX). In vitro, DOX-loaded NPs were expectedly more toxic to zebrafish melanoma (Zmel) cells than no-DOX NPs. In zebrafish embryos, the NPs were rapidly distributed through blood circulation and were found to colocalize over time with the vascular endothelium and local macrophages. Suprisingly, the NPs efficiently reduced the outgrowth of Zmel tumoral masses in an experimental metastasis assay in zebrafish embryos regardless of their loading with DOX. The anti-metastatic effect of these NPs was further improved by increasing the amount of HSA coating, also resulting in higher embryo survival. Altogether, IO STMS NPs showed promising cytotoxic effects on a relevant zebrafish metastasis model, inhibiting metastatic outgrowth in vivo independently of the drug loading. This opens the door to further testing for better exploiting their targeting and drug delivery potentialities.
Fil: Asokan, Nandini. Inserm; Francia
Fil: Mittelheisser, Vincent. Inserm; Francia
Fil: Jablonski, Arnaud. Université de Strasbourg; Francia
Fil: Adam, Alexandre. Université de Strasbourg; Francia
Fil: Bizeau, Joëlle. Université de Strasbourg; Francia
Fil: Harlepp, Sébastien. Inserm; Francia
Fil: Hyenne, Vincent. Inserm; Francia
Fil: Lefebvre, Olivier. Inserm; Francia
Fil: Goetz, Jacky. Inserm; Francia
Fil: Tasso, Mariana Patricia. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mertz, Damien. Université de Strasbourg; Francia
description Mesoporous silica-based nanoparticles (NPs) are promising tools for developing targeted therapeutic interventions in cancer. Endowed with a large pore silica shell suitable for drug encapsulation and with a responsive magnetic core, iron oxide stellate mesoporous silica (IO STMS) NPs stand out. Yet, their impact and potential toxicity on relevant in vivo models has not been carefully tested yet. Herein, we assessed the impact of these IO STMS nanocomposites in a syngeneic metastasis assay in zebrafish. NPs were surface-modified with human serum albumin (HSA) and loaded or not with the chemotherapeutic doxorubicin (DOX). In vitro, DOX-loaded NPs were expectedly more toxic to zebrafish melanoma (Zmel) cells than no-DOX NPs. In zebrafish embryos, the NPs were rapidly distributed through blood circulation and were found to colocalize over time with the vascular endothelium and local macrophages. Suprisingly, the NPs efficiently reduced the outgrowth of Zmel tumoral masses in an experimental metastasis assay in zebrafish embryos regardless of their loading with DOX. The anti-metastatic effect of these NPs was further improved by increasing the amount of HSA coating, also resulting in higher embryo survival. Altogether, IO STMS NPs showed promising cytotoxic effects on a relevant zebrafish metastasis model, inhibiting metastatic outgrowth in vivo independently of the drug loading. This opens the door to further testing for better exploiting their targeting and drug delivery potentialities.
publishDate 2025
dc.date.none.fl_str_mv 2025-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278981
Asokan, Nandini; Mittelheisser, Vincent; Jablonski, Arnaud; Adam, Alexandre; Bizeau, Joëlle; et al.; Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos; Royal Society of Chemistry; Nanoscale; 17; 48; 11-2025; 27959-27974
2040-3364
2040-3372
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278981
identifier_str_mv Asokan, Nandini; Mittelheisser, Vincent; Jablonski, Arnaud; Adam, Alexandre; Bizeau, Joëlle; et al.; Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos; Royal Society of Chemistry; Nanoscale; 17; 48; 11-2025; 27959-27974
2040-3364
2040-3372
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2025/NR/D5NR02444G
info:eu-repo/semantics/altIdentifier/doi/10.1039/D5NR02444G
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.106097

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