Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda

Autores
Alvarez, Analia; Pera, Licia Maria; Virla, Eduardo Gabriel; Baigori, Mario Domingo
Año de publicación
2008
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction: Bacillus thuringiensis (Bt) produces entomopathogenic Cry proteins which are activated to pathogenic form by proteases. Objective: Determination of cry genes and proteolytic activity in three native Bt strains. Material and methods: Total DNA was isolated with the CTAB technique. Cry 1 and 2 genes were amplified with general and specifics primers. Proteolytic activity was assayed by using azocasein as substrate. Results and Discussion: Specific cry genes and the biomass-bound protease activity are showed in the table. Bt RT displayed different cry specific content than the others strains. In addition to Cry protein, Bt is also an excellent source of protease activities. Bt RT has a proteolytic activity significantly different to that achieved for the reference strain Bt 4D1 (P> 0.05, Tukey test). This work was supported by grants PICTO-UNT 761 and PIP 6062 (CONICET).
Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXV Jornadas Científicas de la Asociación de Biología de Tucumán
Tafi del Valle
Argentina
Asociacion de Biologia de Tucuman
Materia
Bacillus thuringiensis
Cry
Genotipo
Accion insecticida
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195323
Acceder
id CONICETDig_e22b92983bc62cf0a4e66f806df41b1a
oai_identifier_str oai:ri.conicet.gov.ar:11336/195323
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperdaAlvarez, AnaliaPera, Licia MariaVirla, Eduardo GabrielBaigori, Mario DomingoBacillus thuringiensisCryGenotipoAccion insecticidahttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Introduction: Bacillus thuringiensis (Bt) produces entomopathogenic Cry proteins which are activated to pathogenic form by proteases. Objective: Determination of cry genes and proteolytic activity in three native Bt strains. Material and methods: Total DNA was isolated with the CTAB technique. Cry 1 and 2 genes were amplified with general and specifics primers. Proteolytic activity was assayed by using azocasein as substrate. Results and Discussion: Specific cry genes and the biomass-bound protease activity are showed in the table. Bt RT displayed different cry specific content than the others strains. In addition to Cry protein, Bt is also an excellent source of protease activities. Bt RT has a proteolytic activity significantly different to that achieved for the reference strain Bt 4D1 (P> 0.05, Tukey test). This work was supported by grants PICTO-UNT 761 and PIP 6062 (CONICET).Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXXV Jornadas Científicas de la Asociación de Biología de TucumánTafi del ValleArgentinaAsociacion de Biologia de TucumanTech Science Press2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195323Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda; XXV Jornadas Científicas de la Asociación de Biología de Tucumán; Tafi del Valle; Argentina; 2008; 151-1510327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1503955485_QmlvY2VsbCBYWFYgSkMgMjAwOC5wZGY=.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:40Zoai:ri.conicet.gov.ar:11336/195323instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:40.489CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda
title Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda
spellingShingle Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda
Alvarez, Analia
Bacillus thuringiensis
Cry
Genotipo
Accion insecticida
title_short Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda
title_full Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda
title_fullStr Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda
title_full_unstemmed Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda
title_sort Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda
dc.creator.none.fl_str_mv Alvarez, Analia
Pera, Licia Maria
Virla, Eduardo Gabriel
Baigori, Mario Domingo
author Alvarez, Analia
author_facet Alvarez, Analia
Pera, Licia Maria
Virla, Eduardo Gabriel
Baigori, Mario Domingo
author_role author
author2 Pera, Licia Maria
Virla, Eduardo Gabriel
Baigori, Mario Domingo
author2_role author
author
author
dc.subject.none.fl_str_mv Bacillus thuringiensis
Cry
Genotipo
Accion insecticida
topic Bacillus thuringiensis
Cry
Genotipo
Accion insecticida
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Introduction: Bacillus thuringiensis (Bt) produces entomopathogenic Cry proteins which are activated to pathogenic form by proteases. Objective: Determination of cry genes and proteolytic activity in three native Bt strains. Material and methods: Total DNA was isolated with the CTAB technique. Cry 1 and 2 genes were amplified with general and specifics primers. Proteolytic activity was assayed by using azocasein as substrate. Results and Discussion: Specific cry genes and the biomass-bound protease activity are showed in the table. Bt RT displayed different cry specific content than the others strains. In addition to Cry protein, Bt is also an excellent source of protease activities. Bt RT has a proteolytic activity significantly different to that achieved for the reference strain Bt 4D1 (P> 0.05, Tukey test). This work was supported by grants PICTO-UNT 761 and PIP 6062 (CONICET).
Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXV Jornadas Científicas de la Asociación de Biología de Tucumán
Tafi del Valle
Argentina
Asociacion de Biologia de Tucuman
description Introduction: Bacillus thuringiensis (Bt) produces entomopathogenic Cry proteins which are activated to pathogenic form by proteases. Objective: Determination of cry genes and proteolytic activity in three native Bt strains. Material and methods: Total DNA was isolated with the CTAB technique. Cry 1 and 2 genes were amplified with general and specifics primers. Proteolytic activity was assayed by using azocasein as substrate. Results and Discussion: Specific cry genes and the biomass-bound protease activity are showed in the table. Bt RT displayed different cry specific content than the others strains. In addition to Cry protein, Bt is also an excellent source of protease activities. Bt RT has a proteolytic activity significantly different to that achieved for the reference strain Bt 4D1 (P> 0.05, Tukey test). This work was supported by grants PICTO-UNT 761 and PIP 6062 (CONICET).
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195323
Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda; XXV Jornadas Científicas de la Asociación de Biología de Tucumán; Tafi del Valle; Argentina; 2008; 151-151
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195323
identifier_str_mv Cry genes profile and proteolytic activity of native Bacillus thuringiensis strains against Spodoptera frugiperda; XXV Jornadas Científicas de la Asociación de Biología de Tucumán; Tafi del Valle; Argentina; 2008; 151-151
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1503955485_QmlvY2VsbCBYWFYgSkMgMjAwOC5wZGY=.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269174875815936
score 13.13397

Publicaciones similares