Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)

Autores
Alvarez, Analia; Pera, Licia Maria; Virla, Eduardo Gabriel; Baigori, Mario Domingo
Año de publicación
2007
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction. The fall armyworm, Spodoptera frugiperda (Sf), is one of the most important corn pests in tropical and subtropical America. Some microorganisms are important candidates for its control. Bacillus thuringiensis (Bt) is an entomopathogenic sporeforming bacterium, which produces proteinaceous crystalline parasporal inclusions (Cry). Objective. Evaluation of ten “Cry” producer strains against first instar larvae of Sf. Materials and methods. Individuals comes from a laboratory colony maintained under controlled conditions (25°C, 70-75% RH and 12:12 L/D). The artificial diet was immersed in sterile water “Cry” protein suspension. Microorganisms isolated from soil and dead larvae were used. Larval mortality was determined during 7 days. Results and conclusions. One of the native strains tested, called RT3, had a strong toxicity against Sf larvae. Considering two independent assays, a mortality level from 76.6 (F=60.50; gl=1; P=0.0015) to 100% was observed after 3 and 4 days, respectively. Finally, in the first assay no larva was survived. Larval mortality using serial dilution of “Cry” suspension was also determined. According to these results, the isolated RT3 can be considered an active strain. This work were supported by grant CIUNT D308 and CONICET
Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXII Jornadas Científicas de la Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
Materia
SPODOPTERA FRUGIPERDA
BACILLUS THURINGIENSIS
ENTOMOPATHOGENS
BIOCONTROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199629
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/199629
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)Alvarez, AnaliaPera, Licia MariaVirla, Eduardo GabrielBaigori, Mario DomingoSPODOPTERA FRUGIPERDABACILLUS THURINGIENSISENTOMOPATHOGENSBIOCONTROLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction. The fall armyworm, Spodoptera frugiperda (Sf), is one of the most important corn pests in tropical and subtropical America. Some microorganisms are important candidates for its control. Bacillus thuringiensis (Bt) is an entomopathogenic sporeforming bacterium, which produces proteinaceous crystalline parasporal inclusions (Cry). Objective. Evaluation of ten “Cry” producer strains against first instar larvae of Sf. Materials and methods. Individuals comes from a laboratory colony maintained under controlled conditions (25°C, 70-75% RH and 12:12 L/D). The artificial diet was immersed in sterile water “Cry” protein suspension. Microorganisms isolated from soil and dead larvae were used. Larval mortality was determined during 7 days. Results and conclusions. One of the native strains tested, called RT3, had a strong toxicity against Sf larvae. Considering two independent assays, a mortality level from 76.6 (F=60.50; gl=1; P=0.0015) to 100% was observed after 3 and 4 days, respectively. Finally, in the first assay no larva was survived. Larval mortality using serial dilution of “Cry” suspension was also determined. According to these results, the isolated RT3 can be considered an active strain. This work were supported by grant CIUNT D308 and CONICETFil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXXII Jornadas Científicas de la Asociación de Biología de TucumánTafí del ValleArgentinaAsociación de Biología de TucumánTech Science Press2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199629Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae); XXII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2005; 200-2000327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://asobioltuc.com/uploads/archivos/1503956056_QmlvY2VsbCBYWElJIEpDIDIwMDUucGRm.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:36Zoai:ri.conicet.gov.ar:11336/199629instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:36.307CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)
title Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)
spellingShingle Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)
Alvarez, Analia
SPODOPTERA FRUGIPERDA
BACILLUS THURINGIENSIS
ENTOMOPATHOGENS
BIOCONTROL
title_short Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)
title_full Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)
title_fullStr Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)
title_full_unstemmed Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)
title_sort Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae)
dc.creator.none.fl_str_mv Alvarez, Analia
Pera, Licia Maria
Virla, Eduardo Gabriel
Baigori, Mario Domingo
author Alvarez, Analia
author_facet Alvarez, Analia
Pera, Licia Maria
Virla, Eduardo Gabriel
Baigori, Mario Domingo
author_role author
author2 Pera, Licia Maria
Virla, Eduardo Gabriel
Baigori, Mario Domingo
author2_role author
author
author
dc.subject.none.fl_str_mv SPODOPTERA FRUGIPERDA
BACILLUS THURINGIENSIS
ENTOMOPATHOGENS
BIOCONTROL
topic SPODOPTERA FRUGIPERDA
BACILLUS THURINGIENSIS
ENTOMOPATHOGENS
BIOCONTROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction. The fall armyworm, Spodoptera frugiperda (Sf), is one of the most important corn pests in tropical and subtropical America. Some microorganisms are important candidates for its control. Bacillus thuringiensis (Bt) is an entomopathogenic sporeforming bacterium, which produces proteinaceous crystalline parasporal inclusions (Cry). Objective. Evaluation of ten “Cry” producer strains against first instar larvae of Sf. Materials and methods. Individuals comes from a laboratory colony maintained under controlled conditions (25°C, 70-75% RH and 12:12 L/D). The artificial diet was immersed in sterile water “Cry” protein suspension. Microorganisms isolated from soil and dead larvae were used. Larval mortality was determined during 7 days. Results and conclusions. One of the native strains tested, called RT3, had a strong toxicity against Sf larvae. Considering two independent assays, a mortality level from 76.6 (F=60.50; gl=1; P=0.0015) to 100% was observed after 3 and 4 days, respectively. Finally, in the first assay no larva was survived. Larval mortality using serial dilution of “Cry” suspension was also determined. According to these results, the isolated RT3 can be considered an active strain. This work were supported by grant CIUNT D308 and CONICET
Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXII Jornadas Científicas de la Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
description Introduction. The fall armyworm, Spodoptera frugiperda (Sf), is one of the most important corn pests in tropical and subtropical America. Some microorganisms are important candidates for its control. Bacillus thuringiensis (Bt) is an entomopathogenic sporeforming bacterium, which produces proteinaceous crystalline parasporal inclusions (Cry). Objective. Evaluation of ten “Cry” producer strains against first instar larvae of Sf. Materials and methods. Individuals comes from a laboratory colony maintained under controlled conditions (25°C, 70-75% RH and 12:12 L/D). The artificial diet was immersed in sterile water “Cry” protein suspension. Microorganisms isolated from soil and dead larvae were used. Larval mortality was determined during 7 days. Results and conclusions. One of the native strains tested, called RT3, had a strong toxicity against Sf larvae. Considering two independent assays, a mortality level from 76.6 (F=60.50; gl=1; P=0.0015) to 100% was observed after 3 and 4 days, respectively. Finally, in the first assay no larva was survived. Larval mortality using serial dilution of “Cry” suspension was also determined. According to these results, the isolated RT3 can be considered an active strain. This work were supported by grant CIUNT D308 and CONICET
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199629
Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae); XXII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2005; 200-200
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199629
identifier_str_mv Isolation of Bacillus strain toxic to Spodoptera frugiperda (Lep.: Noctuidae); XXII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2005; 200-200
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://asobioltuc.com/uploads/archivos/1503956056_QmlvY2VsbCBYWElJIEpDIDIwMDUucGRm.pdf
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eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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