Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda

Autores
Alvarez, Analia; Loto, Flavia del Valle; Nieto, Carlos Daniel; Pera, Licia Maria; Virla, Eduardo Gabriel; Baigori, Mario Domingo
Año de publicación
2008
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction: Bacillus thuringiensis (Bt) are the microorganisms most widely used in biological control of insect pest. These bacteria produce entomophatogenic crystals (Cry) during sporulation showing different morphologies according to Bt strains. Objectives: To determine the morphology of crystals produced by threenative Bt strains active against larvae of Spodoptera frugiperda (Sf) and to characterize the microorganisms molecularly. Materials and methods: Crystals were studied by scanning electron microscopy. Sporulated cells were removed from the agarized medium, washed and placed on a micro slide. The 16S rDNA gene was amplified from genomic DNA by PCR. Results and conclusions: Morphological observations showed that the three strains contained bipyramidal crystals of two different sizes. This feature may resemble those against lepidopteran targets. The 16S rDNA analysis identifies the isolations as B. thuringiensis subspecies. The sequences were deposited in the Gene Bank, access Nº: RT EF638795, LSM EF638796 an LQ EF638798. These results complement the knowledge on these native pathogenic strains with promissory characteristics for Sf biological control. Acknowledgments: PICTO-UNT 761, PIP 6062 and CIUNT 26/ D308.
Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Nieto, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
I Reunión Conjunta de Sociedades de Biología de la República Argentina
Huerta Grande
Argentina
Sociedad Argentina de Biología
Sociedad de Biología de Cuyo
Sociedad de Biología de Córdoba
Materia
CRYSTAL TOXIN
BACILLUS THURINGIENSIS
BIOCONTROL
SPODOPTERA FRUGIPERDA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/196152
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/196152
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperdaAlvarez, AnaliaLoto, Flavia del ValleNieto, Carlos DanielPera, Licia MariaVirla, Eduardo GabrielBaigori, Mario DomingoCRYSTAL TOXINBACILLUS THURINGIENSISBIOCONTROLSPODOPTERA FRUGIPERDAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: Bacillus thuringiensis (Bt) are the microorganisms most widely used in biological control of insect pest. These bacteria produce entomophatogenic crystals (Cry) during sporulation showing different morphologies according to Bt strains. Objectives: To determine the morphology of crystals produced by threenative Bt strains active against larvae of Spodoptera frugiperda (Sf) and to characterize the microorganisms molecularly. Materials and methods: Crystals were studied by scanning electron microscopy. Sporulated cells were removed from the agarized medium, washed and placed on a micro slide. The 16S rDNA gene was amplified from genomic DNA by PCR. Results and conclusions: Morphological observations showed that the three strains contained bipyramidal crystals of two different sizes. This feature may resemble those against lepidopteran targets. The 16S rDNA analysis identifies the isolations as B. thuringiensis subspecies. The sequences were deposited in the Gene Bank, access Nº: RT EF638795, LSM EF638796 an LQ EF638798. These results complement the knowledge on these native pathogenic strains with promissory characteristics for Sf biological control. Acknowledgments: PICTO-UNT 761, PIP 6062 and CIUNT 26/ D308.Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Nieto, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaI Reunión Conjunta de Sociedades de Biología de la República ArgentinaHuerta GrandeArgentinaSociedad Argentina de BiologíaSociedad de Biología de CuyoSociedad de Biología de CórdobaTech Science Press2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196152Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda; I Reunión Conjunta de Sociedades de Biología de la República Argentina; Huerta Grande; Argentina; 2007; 82-820327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v32nSuppl.S/37978Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:25Zoai:ri.conicet.gov.ar:11336/196152instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:26.845CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda
title Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda
spellingShingle Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda
Alvarez, Analia
CRYSTAL TOXIN
BACILLUS THURINGIENSIS
BIOCONTROL
SPODOPTERA FRUGIPERDA
title_short Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda
title_full Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda
title_fullStr Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda
title_full_unstemmed Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda
title_sort Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda
dc.creator.none.fl_str_mv Alvarez, Analia
Loto, Flavia del Valle
Nieto, Carlos Daniel
Pera, Licia Maria
Virla, Eduardo Gabriel
Baigori, Mario Domingo
author Alvarez, Analia
author_facet Alvarez, Analia
Loto, Flavia del Valle
Nieto, Carlos Daniel
Pera, Licia Maria
Virla, Eduardo Gabriel
Baigori, Mario Domingo
author_role author
author2 Loto, Flavia del Valle
Nieto, Carlos Daniel
Pera, Licia Maria
Virla, Eduardo Gabriel
Baigori, Mario Domingo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CRYSTAL TOXIN
BACILLUS THURINGIENSIS
BIOCONTROL
SPODOPTERA FRUGIPERDA
topic CRYSTAL TOXIN
BACILLUS THURINGIENSIS
BIOCONTROL
SPODOPTERA FRUGIPERDA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: Bacillus thuringiensis (Bt) are the microorganisms most widely used in biological control of insect pest. These bacteria produce entomophatogenic crystals (Cry) during sporulation showing different morphologies according to Bt strains. Objectives: To determine the morphology of crystals produced by threenative Bt strains active against larvae of Spodoptera frugiperda (Sf) and to characterize the microorganisms molecularly. Materials and methods: Crystals were studied by scanning electron microscopy. Sporulated cells were removed from the agarized medium, washed and placed on a micro slide. The 16S rDNA gene was amplified from genomic DNA by PCR. Results and conclusions: Morphological observations showed that the three strains contained bipyramidal crystals of two different sizes. This feature may resemble those against lepidopteran targets. The 16S rDNA analysis identifies the isolations as B. thuringiensis subspecies. The sequences were deposited in the Gene Bank, access Nº: RT EF638795, LSM EF638796 an LQ EF638798. These results complement the knowledge on these native pathogenic strains with promissory characteristics for Sf biological control. Acknowledgments: PICTO-UNT 761, PIP 6062 and CIUNT 26/ D308.
Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Nieto, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
I Reunión Conjunta de Sociedades de Biología de la República Argentina
Huerta Grande
Argentina
Sociedad Argentina de Biología
Sociedad de Biología de Cuyo
Sociedad de Biología de Córdoba
description Introduction: Bacillus thuringiensis (Bt) are the microorganisms most widely used in biological control of insect pest. These bacteria produce entomophatogenic crystals (Cry) during sporulation showing different morphologies according to Bt strains. Objectives: To determine the morphology of crystals produced by threenative Bt strains active against larvae of Spodoptera frugiperda (Sf) and to characterize the microorganisms molecularly. Materials and methods: Crystals were studied by scanning electron microscopy. Sporulated cells were removed from the agarized medium, washed and placed on a micro slide. The 16S rDNA gene was amplified from genomic DNA by PCR. Results and conclusions: Morphological observations showed that the three strains contained bipyramidal crystals of two different sizes. This feature may resemble those against lepidopteran targets. The 16S rDNA analysis identifies the isolations as B. thuringiensis subspecies. The sequences were deposited in the Gene Bank, access Nº: RT EF638795, LSM EF638796 an LQ EF638798. These results complement the knowledge on these native pathogenic strains with promissory characteristics for Sf biological control. Acknowledgments: PICTO-UNT 761, PIP 6062 and CIUNT 26/ D308.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/196152
Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda; I Reunión Conjunta de Sociedades de Biología de la República Argentina; Huerta Grande; Argentina; 2007; 82-82
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/196152
identifier_str_mv Molecular characterization and crystal morphology of indigenous entomophatogenic Bacillus strains against Spodoptera frugiperda; I Reunión Conjunta de Sociedades de Biología de la República Argentina; Huerta Grande; Argentina; 2007; 82-82
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v32nSuppl.S/37978
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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