Construction of a consensus genetic map of Eragrostis curvula

Autores
Gallardo, Jimena Alicia; Gallo, Cristian Andrés; Sansot Puleston, Mateo; Echenique, Carmen Viviana; Zappacosta, Diego Carlos
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Eragrostis curvula is a forage grass used by our group as a model for the study of displosporous apomixis. It includes genotypes with different ploidy levels (from 2X to 8X, with X = 10) and reproductive modes (sexual and facultative or obligate apomixis). Previously, our group built the first highly saturated genetic linkage map of E. curvula using a mapping population generated from the cross between two tetraploid genotypes, OTA-S and Don Walter. At that time, the small size of the mapping population (61 individuals) did not allow the generation of a consensus map between the maternal and paternal maps. However, a map was constructed for each parental genotype and the apomeiosis-linked locus (APO-locus) was located in the paternal map. The objective of this work was to expand the mapping population using the same parental lines in order to build a high-density consensus genetic map where to position the APO-locus and to make syntenic analysis. The new mapping population consisted of 109 hybrid individuals coming from the previous population and new ones coming from the new crosses. This population was phenotyped by cytoembryology and through the use of a dominant molecular marker linked to apomeiosis, resulting in 48 sexual offprings and 61 apomictic ones. The genotyping was performed with SNPs using the DArT-Seq technology (SAGA-CIMMYT, Mexico). For the map construction single dose allele markers and biparental SNPs markers were used. Linkage maps for each parent and a consensus map were built. The consensus genetic map contains a total of 1,132 markers, of which 587 were paternal, 514 maternal and 31 biparental. The total length of the linkage map was 4,605 cM and it was made up of 20 linkage group (LG). Synteny analysis was performed using the genome assembly of E. curvula (cv. Victoria). In the paternal map, a region delimited by three markers was found that cosegregates with apomeiosis (APO-locus). The APO-locus delimited a region of 11,322,729 bp in the reference genome of cv. Victoria. The construction of this mapping population, added to the of E. curvula consensus map are valuable elements for future studies of apomixis.
Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Gallo, Cristian Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Sansot Puleston, Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
IV International Congress on Apomixis Research
Rosario
Argentina
Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario
Materia
CONSENSOUS GENETIC MAP
APO-LOCUS
MAPPING POPULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/240413
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Construction of a consensus genetic map of Eragrostis curvulaGallardo, Jimena AliciaGallo, Cristian AndrésSansot Puleston, MateoEchenique, Carmen VivianaZappacosta, Diego CarlosCONSENSOUS GENETIC MAPAPO-LOCUSMAPPING POPULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Eragrostis curvula is a forage grass used by our group as a model for the study of displosporous apomixis. It includes genotypes with different ploidy levels (from 2X to 8X, with X = 10) and reproductive modes (sexual and facultative or obligate apomixis). Previously, our group built the first highly saturated genetic linkage map of E. curvula using a mapping population generated from the cross between two tetraploid genotypes, OTA-S and Don Walter. At that time, the small size of the mapping population (61 individuals) did not allow the generation of a consensus map between the maternal and paternal maps. However, a map was constructed for each parental genotype and the apomeiosis-linked locus (APO-locus) was located in the paternal map. The objective of this work was to expand the mapping population using the same parental lines in order to build a high-density consensus genetic map where to position the APO-locus and to make syntenic analysis. The new mapping population consisted of 109 hybrid individuals coming from the previous population and new ones coming from the new crosses. This population was phenotyped by cytoembryology and through the use of a dominant molecular marker linked to apomeiosis, resulting in 48 sexual offprings and 61 apomictic ones. The genotyping was performed with SNPs using the DArT-Seq technology (SAGA-CIMMYT, Mexico). For the map construction single dose allele markers and biparental SNPs markers were used. Linkage maps for each parent and a consensus map were built. The consensus genetic map contains a total of 1,132 markers, of which 587 were paternal, 514 maternal and 31 biparental. The total length of the linkage map was 4,605 cM and it was made up of 20 linkage group (LG). Synteny analysis was performed using the genome assembly of E. curvula (cv. Victoria). In the paternal map, a region delimited by three markers was found that cosegregates with apomeiosis (APO-locus). The APO-locus delimited a region of 11,322,729 bp in the reference genome of cv. Victoria. The construction of this mapping population, added to the of E. curvula consensus map are valuable elements for future studies of apomixis.Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Gallo, Cristian Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Sansot Puleston, Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaIV International Congress on Apomixis ResearchRosarioArgentinaUniversidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de RosarioUniversidad Nacional de Rosario. Facultad de Ciencias Agrarias2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240413Construction of a consensus genetic map of Eragrostis curvula; IV International Congress on Apomixis Research; Rosario; Argentina; 2023; 33-33CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://apomixis2023.com.ar/info:eu-repo/semantics/altIdentifier/url/https://apomixis2023.com.ar/wp-content/uploads/APOMIXIS-2023-book-of-abstracts.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:47:26Zoai:ri.conicet.gov.ar:11336/240413instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:47:26.484CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Construction of a consensus genetic map of Eragrostis curvula
title Construction of a consensus genetic map of Eragrostis curvula
spellingShingle Construction of a consensus genetic map of Eragrostis curvula
Gallardo, Jimena Alicia
CONSENSOUS GENETIC MAP
APO-LOCUS
MAPPING POPULATION
title_short Construction of a consensus genetic map of Eragrostis curvula
title_full Construction of a consensus genetic map of Eragrostis curvula
title_fullStr Construction of a consensus genetic map of Eragrostis curvula
title_full_unstemmed Construction of a consensus genetic map of Eragrostis curvula
title_sort Construction of a consensus genetic map of Eragrostis curvula
dc.creator.none.fl_str_mv Gallardo, Jimena Alicia
Gallo, Cristian Andrés
Sansot Puleston, Mateo
Echenique, Carmen Viviana
Zappacosta, Diego Carlos
author Gallardo, Jimena Alicia
author_facet Gallardo, Jimena Alicia
Gallo, Cristian Andrés
Sansot Puleston, Mateo
Echenique, Carmen Viviana
Zappacosta, Diego Carlos
author_role author
author2 Gallo, Cristian Andrés
Sansot Puleston, Mateo
Echenique, Carmen Viviana
Zappacosta, Diego Carlos
author2_role author
author
author
author
dc.subject.none.fl_str_mv CONSENSOUS GENETIC MAP
APO-LOCUS
MAPPING POPULATION
topic CONSENSOUS GENETIC MAP
APO-LOCUS
MAPPING POPULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Eragrostis curvula is a forage grass used by our group as a model for the study of displosporous apomixis. It includes genotypes with different ploidy levels (from 2X to 8X, with X = 10) and reproductive modes (sexual and facultative or obligate apomixis). Previously, our group built the first highly saturated genetic linkage map of E. curvula using a mapping population generated from the cross between two tetraploid genotypes, OTA-S and Don Walter. At that time, the small size of the mapping population (61 individuals) did not allow the generation of a consensus map between the maternal and paternal maps. However, a map was constructed for each parental genotype and the apomeiosis-linked locus (APO-locus) was located in the paternal map. The objective of this work was to expand the mapping population using the same parental lines in order to build a high-density consensus genetic map where to position the APO-locus and to make syntenic analysis. The new mapping population consisted of 109 hybrid individuals coming from the previous population and new ones coming from the new crosses. This population was phenotyped by cytoembryology and through the use of a dominant molecular marker linked to apomeiosis, resulting in 48 sexual offprings and 61 apomictic ones. The genotyping was performed with SNPs using the DArT-Seq technology (SAGA-CIMMYT, Mexico). For the map construction single dose allele markers and biparental SNPs markers were used. Linkage maps for each parent and a consensus map were built. The consensus genetic map contains a total of 1,132 markers, of which 587 were paternal, 514 maternal and 31 biparental. The total length of the linkage map was 4,605 cM and it was made up of 20 linkage group (LG). Synteny analysis was performed using the genome assembly of E. curvula (cv. Victoria). In the paternal map, a region delimited by three markers was found that cosegregates with apomeiosis (APO-locus). The APO-locus delimited a region of 11,322,729 bp in the reference genome of cv. Victoria. The construction of this mapping population, added to the of E. curvula consensus map are valuable elements for future studies of apomixis.
Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Gallo, Cristian Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Sansot Puleston, Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
IV International Congress on Apomixis Research
Rosario
Argentina
Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario
description Eragrostis curvula is a forage grass used by our group as a model for the study of displosporous apomixis. It includes genotypes with different ploidy levels (from 2X to 8X, with X = 10) and reproductive modes (sexual and facultative or obligate apomixis). Previously, our group built the first highly saturated genetic linkage map of E. curvula using a mapping population generated from the cross between two tetraploid genotypes, OTA-S and Don Walter. At that time, the small size of the mapping population (61 individuals) did not allow the generation of a consensus map between the maternal and paternal maps. However, a map was constructed for each parental genotype and the apomeiosis-linked locus (APO-locus) was located in the paternal map. The objective of this work was to expand the mapping population using the same parental lines in order to build a high-density consensus genetic map where to position the APO-locus and to make syntenic analysis. The new mapping population consisted of 109 hybrid individuals coming from the previous population and new ones coming from the new crosses. This population was phenotyped by cytoembryology and through the use of a dominant molecular marker linked to apomeiosis, resulting in 48 sexual offprings and 61 apomictic ones. The genotyping was performed with SNPs using the DArT-Seq technology (SAGA-CIMMYT, Mexico). For the map construction single dose allele markers and biparental SNPs markers were used. Linkage maps for each parent and a consensus map were built. The consensus genetic map contains a total of 1,132 markers, of which 587 were paternal, 514 maternal and 31 biparental. The total length of the linkage map was 4,605 cM and it was made up of 20 linkage group (LG). Synteny analysis was performed using the genome assembly of E. curvula (cv. Victoria). In the paternal map, a region delimited by three markers was found that cosegregates with apomeiosis (APO-locus). The APO-locus delimited a region of 11,322,729 bp in the reference genome of cv. Victoria. The construction of this mapping population, added to the of E. curvula consensus map are valuable elements for future studies of apomixis.
publishDate 2024
dc.date.none.fl_str_mv 2024
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status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240413
Construction of a consensus genetic map of Eragrostis curvula; IV International Congress on Apomixis Research; Rosario; Argentina; 2023; 33-33
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240413
identifier_str_mv Construction of a consensus genetic map of Eragrostis curvula; IV International Congress on Apomixis Research; Rosario; Argentina; 2023; 33-33
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://apomixis2023.com.ar/
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad Nacional de Rosario. Facultad de Ciencias Agrarias
publisher.none.fl_str_mv Universidad Nacional de Rosario. Facultad de Ciencias Agrarias
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