Development of KASP markers linked to apomixis in Eragrostis curvula
- Autores
- Gallardo, Jimena Alicia; Gallo, Cristian Andrés; Rodrigo, Juan Manuel; Echenique, Carmen Viviana; Zappacosta, Diego Carlos
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Recently, our working group has built a new linkage map of the diplosporous grass Eragrostis curvula. For this purpose, a mapping population composed of 109 individuals was generated from the cross between the tetraploids OTA (sexual) and Don Walter (facultative apomictic pollen donor). This new population was genotyped with SNPs markers obtained through the DArT-seq technology. This technique has multiple advantages over other similar sequencing techniques (eg. GBS), since DArT-seq generates thousands of markers with high efficiency, due to the specific pipeline, the high fidelity restriction enzymes used and the intrinsic system of duplicate samples. Like other techniques, multiple pooled samples can be analyzed, reducing costs and time required, and it is possible to work without a reference genome.In this new E. curvula linkage map it was possible to identify a region associated with apomeiosis (APO-locus) and three closely linked (100%) SNPs markers. Primers designed from the sequence of these SNPs allowed the development of KASP (Kompetitive allele specific PCR) markers able to differentiate between sexual and apomictic E. curvula plants (phenotyping).KASP are codominant markers and the results obtained can be observed by fluorescence at the end of a PCR in a PHERAstar Plus equipment available at the GENeTyC laboratory (CERZOS-CONICET). First, the sequence of each SNP markers linked to the APO-locus was identified (69 bp) and the most appropriate regions for primer design were chosen. The Primer3Plus program was used and 5 sets of KASP primers were designed with specific "tails'' for each one of the FAM and HEX alleles for the paternal and maternal alleles, respectively. These 5 sets of KASP markers were tested on the population parental lines and on a set of sexual and apomictic cultivars (20). Based on its efficiency one of these KASPs markers (K277) was selected and used to validate the phenotype of the offspring. The KASP marker designed, despite being codominant, demonstrated its reliability to perform the phenotypic characterization, distinguishing between sexual and apomictic individuals. Sequences corresponding to SNP markers 100% linked to apomeiosis showed homology with genes, pointing at such genes as potential candidates to be involved in the regulation of the reproductive mode of this grass.
Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Gallo, Cristian Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
IV International Congress on Apomixis Research
Rosario
Argentina
Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario - Materia
-
KASP MARKERS
PHENOTYPIC CHARACTERIZATION
APO-LOCUS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/234160
Ver los metadatos del registro completo
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Development of KASP markers linked to apomixis in Eragrostis curvulaGallardo, Jimena AliciaGallo, Cristian AndrésRodrigo, Juan ManuelEchenique, Carmen VivianaZappacosta, Diego CarlosKASP MARKERSPHENOTYPIC CHARACTERIZATIONAPO-LOCUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Recently, our working group has built a new linkage map of the diplosporous grass Eragrostis curvula. For this purpose, a mapping population composed of 109 individuals was generated from the cross between the tetraploids OTA (sexual) and Don Walter (facultative apomictic pollen donor). This new population was genotyped with SNPs markers obtained through the DArT-seq technology. This technique has multiple advantages over other similar sequencing techniques (eg. GBS), since DArT-seq generates thousands of markers with high efficiency, due to the specific pipeline, the high fidelity restriction enzymes used and the intrinsic system of duplicate samples. Like other techniques, multiple pooled samples can be analyzed, reducing costs and time required, and it is possible to work without a reference genome.In this new E. curvula linkage map it was possible to identify a region associated with apomeiosis (APO-locus) and three closely linked (100%) SNPs markers. Primers designed from the sequence of these SNPs allowed the development of KASP (Kompetitive allele specific PCR) markers able to differentiate between sexual and apomictic E. curvula plants (phenotyping).KASP are codominant markers and the results obtained can be observed by fluorescence at the end of a PCR in a PHERAstar Plus equipment available at the GENeTyC laboratory (CERZOS-CONICET). First, the sequence of each SNP markers linked to the APO-locus was identified (69 bp) and the most appropriate regions for primer design were chosen. The Primer3Plus program was used and 5 sets of KASP primers were designed with specific "tails'' for each one of the FAM and HEX alleles for the paternal and maternal alleles, respectively. These 5 sets of KASP markers were tested on the population parental lines and on a set of sexual and apomictic cultivars (20). Based on its efficiency one of these KASPs markers (K277) was selected and used to validate the phenotype of the offspring. The KASP marker designed, despite being codominant, demonstrated its reliability to perform the phenotypic characterization, distinguishing between sexual and apomictic individuals. Sequences corresponding to SNP markers 100% linked to apomeiosis showed homology with genes, pointing at such genes as potential candidates to be involved in the regulation of the reproductive mode of this grass.Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Gallo, Cristian Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaIV International Congress on Apomixis ResearchRosarioArgentinaUniversidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de RosarioUniversidad Nacional de Rosario. Facultad de Ciencias Agrarias2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/234160Development of KASP markers linked to apomixis in Eragrostis curvula; IV International Congress on Apomixis Research; Rosario; Argentina; 2023; 37-37CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://apomixis2023.com.ar/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:48Zoai:ri.conicet.gov.ar:11336/234160instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:48.754CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Development of KASP markers linked to apomixis in Eragrostis curvula |
| title |
Development of KASP markers linked to apomixis in Eragrostis curvula |
| spellingShingle |
Development of KASP markers linked to apomixis in Eragrostis curvula Gallardo, Jimena Alicia KASP MARKERS PHENOTYPIC CHARACTERIZATION APO-LOCUS |
| title_short |
Development of KASP markers linked to apomixis in Eragrostis curvula |
| title_full |
Development of KASP markers linked to apomixis in Eragrostis curvula |
| title_fullStr |
Development of KASP markers linked to apomixis in Eragrostis curvula |
| title_full_unstemmed |
Development of KASP markers linked to apomixis in Eragrostis curvula |
| title_sort |
Development of KASP markers linked to apomixis in Eragrostis curvula |
| dc.creator.none.fl_str_mv |
Gallardo, Jimena Alicia Gallo, Cristian Andrés Rodrigo, Juan Manuel Echenique, Carmen Viviana Zappacosta, Diego Carlos |
| author |
Gallardo, Jimena Alicia |
| author_facet |
Gallardo, Jimena Alicia Gallo, Cristian Andrés Rodrigo, Juan Manuel Echenique, Carmen Viviana Zappacosta, Diego Carlos |
| author_role |
author |
| author2 |
Gallo, Cristian Andrés Rodrigo, Juan Manuel Echenique, Carmen Viviana Zappacosta, Diego Carlos |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
KASP MARKERS PHENOTYPIC CHARACTERIZATION APO-LOCUS |
| topic |
KASP MARKERS PHENOTYPIC CHARACTERIZATION APO-LOCUS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Recently, our working group has built a new linkage map of the diplosporous grass Eragrostis curvula. For this purpose, a mapping population composed of 109 individuals was generated from the cross between the tetraploids OTA (sexual) and Don Walter (facultative apomictic pollen donor). This new population was genotyped with SNPs markers obtained through the DArT-seq technology. This technique has multiple advantages over other similar sequencing techniques (eg. GBS), since DArT-seq generates thousands of markers with high efficiency, due to the specific pipeline, the high fidelity restriction enzymes used and the intrinsic system of duplicate samples. Like other techniques, multiple pooled samples can be analyzed, reducing costs and time required, and it is possible to work without a reference genome.In this new E. curvula linkage map it was possible to identify a region associated with apomeiosis (APO-locus) and three closely linked (100%) SNPs markers. Primers designed from the sequence of these SNPs allowed the development of KASP (Kompetitive allele specific PCR) markers able to differentiate between sexual and apomictic E. curvula plants (phenotyping).KASP are codominant markers and the results obtained can be observed by fluorescence at the end of a PCR in a PHERAstar Plus equipment available at the GENeTyC laboratory (CERZOS-CONICET). First, the sequence of each SNP markers linked to the APO-locus was identified (69 bp) and the most appropriate regions for primer design were chosen. The Primer3Plus program was used and 5 sets of KASP primers were designed with specific "tails'' for each one of the FAM and HEX alleles for the paternal and maternal alleles, respectively. These 5 sets of KASP markers were tested on the population parental lines and on a set of sexual and apomictic cultivars (20). Based on its efficiency one of these KASPs markers (K277) was selected and used to validate the phenotype of the offspring. The KASP marker designed, despite being codominant, demonstrated its reliability to perform the phenotypic characterization, distinguishing between sexual and apomictic individuals. Sequences corresponding to SNP markers 100% linked to apomeiosis showed homology with genes, pointing at such genes as potential candidates to be involved in the regulation of the reproductive mode of this grass. Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Gallo, Cristian Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina IV International Congress on Apomixis Research Rosario Argentina Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario |
| description |
Recently, our working group has built a new linkage map of the diplosporous grass Eragrostis curvula. For this purpose, a mapping population composed of 109 individuals was generated from the cross between the tetraploids OTA (sexual) and Don Walter (facultative apomictic pollen donor). This new population was genotyped with SNPs markers obtained through the DArT-seq technology. This technique has multiple advantages over other similar sequencing techniques (eg. GBS), since DArT-seq generates thousands of markers with high efficiency, due to the specific pipeline, the high fidelity restriction enzymes used and the intrinsic system of duplicate samples. Like other techniques, multiple pooled samples can be analyzed, reducing costs and time required, and it is possible to work without a reference genome.In this new E. curvula linkage map it was possible to identify a region associated with apomeiosis (APO-locus) and three closely linked (100%) SNPs markers. Primers designed from the sequence of these SNPs allowed the development of KASP (Kompetitive allele specific PCR) markers able to differentiate between sexual and apomictic E. curvula plants (phenotyping).KASP are codominant markers and the results obtained can be observed by fluorescence at the end of a PCR in a PHERAstar Plus equipment available at the GENeTyC laboratory (CERZOS-CONICET). First, the sequence of each SNP markers linked to the APO-locus was identified (69 bp) and the most appropriate regions for primer design were chosen. The Primer3Plus program was used and 5 sets of KASP primers were designed with specific "tails'' for each one of the FAM and HEX alleles for the paternal and maternal alleles, respectively. These 5 sets of KASP markers were tested on the population parental lines and on a set of sexual and apomictic cultivars (20). Based on its efficiency one of these KASPs markers (K277) was selected and used to validate the phenotype of the offspring. The KASP marker designed, despite being codominant, demonstrated its reliability to perform the phenotypic characterization, distinguishing between sexual and apomictic individuals. Sequences corresponding to SNP markers 100% linked to apomeiosis showed homology with genes, pointing at such genes as potential candidates to be involved in the regulation of the reproductive mode of this grass. |
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2023 |
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2023 |
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http://hdl.handle.net/11336/234160 Development of KASP markers linked to apomixis in Eragrostis curvula; IV International Congress on Apomixis Research; Rosario; Argentina; 2023; 37-37 CONICET Digital CONICET |
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http://hdl.handle.net/11336/234160 |
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Development of KASP markers linked to apomixis in Eragrostis curvula; IV International Congress on Apomixis Research; Rosario; Argentina; 2023; 37-37 CONICET Digital CONICET |
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eng |
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Universidad Nacional de Rosario. Facultad de Ciencias Agrarias |
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Universidad Nacional de Rosario. Facultad de Ciencias Agrarias |
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