Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map

Autores
Hermida, Miguel; Bouza, Carmen; Fernández, Carlos; Sciara, Andres Angel; Rodríguez Ramilo, Silvia T.; Fernández, Jesús; Martinez, Paulino
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The turbot (Scophthalmus maximus) is a species of great relevance to European aquaculture and hence might benefit from enhanced genomic resources. The aim of this study was to integrate all previous turbot mapping resources to obtain a dense consensus genetic map. A total of 514 single nucleotide polymorphisms (SNP) and microsatellite markers from reported turbot genetic maps, were used for map construction. Among these markers, 487 (LOD score > 3), including 39% expressed sequence tag (EST)-linked and 61% anonymous, were integrated in 24 linkage groups. The linkage map comprised a total length of 1414 cM (1274.4 cM framework) with 3.3 cM (3.6 cM framework) inter-marker distance, representing an estimated genomic coverage of ~ 90%. This map was used to refine quantitative trait loci (QTL) screening for sex, because of the relevance of this trait for the turbot industry, which confirmed not only the main sex-determining region at linkage group (LG) 5, but also the involvement of additional significant genetic factors at other linkage groups. Comparative mapping supported the macrosyntenic pattern previously observed when comparing turbot and model fish genomes and enabled identification of candidate genes related to sexual development and reproduction at LG5, such as DNAJC19 and Sox2. The available number of markers in the turbot map could be easily increased to 592 because of the established correspondence between linkage groups of the two main genetic maps, thus approaching 1 marker/Mb based on the turbot genome size. This map represents a useful tool for evolutionary studies and for supporting ongoing marker-assisted selection programs in this species.
Fil: Hermida, Miguel. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España
Fil: Bouza, Carmen. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España
Fil: Fernández, Carlos. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España
Fil: Sciara, Andres Angel. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Rodríguez Ramilo, Silvia T.. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Mejora Genética Animal; España
Fil: Fernández, Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Mejora Genética Animal; España
Fil: Martinez, Paulino. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España
Materia
Turbot
Genetic Map
Consensus
Qtl
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4858
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4858
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic mapHermida, MiguelBouza, CarmenFernández, CarlosSciara, Andres AngelRodríguez Ramilo, Silvia T.Fernández, JesúsMartinez, PaulinoTurbotGenetic MapConsensusQtlhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The turbot (Scophthalmus maximus) is a species of great relevance to European aquaculture and hence might benefit from enhanced genomic resources. The aim of this study was to integrate all previous turbot mapping resources to obtain a dense consensus genetic map. A total of 514 single nucleotide polymorphisms (SNP) and microsatellite markers from reported turbot genetic maps, were used for map construction. Among these markers, 487 (LOD score > 3), including 39% expressed sequence tag (EST)-linked and 61% anonymous, were integrated in 24 linkage groups. The linkage map comprised a total length of 1414 cM (1274.4 cM framework) with 3.3 cM (3.6 cM framework) inter-marker distance, representing an estimated genomic coverage of ~ 90%. This map was used to refine quantitative trait loci (QTL) screening for sex, because of the relevance of this trait for the turbot industry, which confirmed not only the main sex-determining region at linkage group (LG) 5, but also the involvement of additional significant genetic factors at other linkage groups. Comparative mapping supported the macrosyntenic pattern previously observed when comparing turbot and model fish genomes and enabled identification of candidate genes related to sexual development and reproduction at LG5, such as DNAJC19 and Sox2. The available number of markers in the turbot map could be easily increased to 592 because of the established correspondence between linkage groups of the two main genetic maps, thus approaching 1 marker/Mb based on the turbot genome size. This map represents a useful tool for evolutionary studies and for supporting ongoing marker-assisted selection programs in this species.Fil: Hermida, Miguel. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; EspañaFil: Bouza, Carmen. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; EspañaFil: Fernández, Carlos. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; EspañaFil: Sciara, Andres Angel. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Rodríguez Ramilo, Silvia T.. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Mejora Genética Animal; EspañaFil: Fernández, Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Mejora Genética Animal; EspañaFil: Martinez, Paulino. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; EspañaElsevier2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4858Hermida, Miguel; Bouza, Carmen; Fernández, Carlos; Sciara, Andres Angel; Rodríguez Ramilo, Silvia T.; et al.; Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map; Elsevier; Aquaculture; 414-415; 11-2013; 19-250044-8486enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0044848613003785info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2013.07.040info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:20Zoai:ri.conicet.gov.ar:11336/4858instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:20.825CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map
title Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map
spellingShingle Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map
Hermida, Miguel
Turbot
Genetic Map
Consensus
Qtl
title_short Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map
title_full Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map
title_fullStr Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map
title_full_unstemmed Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map
title_sort Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map
dc.creator.none.fl_str_mv Hermida, Miguel
Bouza, Carmen
Fernández, Carlos
Sciara, Andres Angel
Rodríguez Ramilo, Silvia T.
Fernández, Jesús
Martinez, Paulino
author Hermida, Miguel
author_facet Hermida, Miguel
Bouza, Carmen
Fernández, Carlos
Sciara, Andres Angel
Rodríguez Ramilo, Silvia T.
Fernández, Jesús
Martinez, Paulino
author_role author
author2 Bouza, Carmen
Fernández, Carlos
Sciara, Andres Angel
Rodríguez Ramilo, Silvia T.
Fernández, Jesús
Martinez, Paulino
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Turbot
Genetic Map
Consensus
Qtl
topic Turbot
Genetic Map
Consensus
Qtl
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The turbot (Scophthalmus maximus) is a species of great relevance to European aquaculture and hence might benefit from enhanced genomic resources. The aim of this study was to integrate all previous turbot mapping resources to obtain a dense consensus genetic map. A total of 514 single nucleotide polymorphisms (SNP) and microsatellite markers from reported turbot genetic maps, were used for map construction. Among these markers, 487 (LOD score > 3), including 39% expressed sequence tag (EST)-linked and 61% anonymous, were integrated in 24 linkage groups. The linkage map comprised a total length of 1414 cM (1274.4 cM framework) with 3.3 cM (3.6 cM framework) inter-marker distance, representing an estimated genomic coverage of ~ 90%. This map was used to refine quantitative trait loci (QTL) screening for sex, because of the relevance of this trait for the turbot industry, which confirmed not only the main sex-determining region at linkage group (LG) 5, but also the involvement of additional significant genetic factors at other linkage groups. Comparative mapping supported the macrosyntenic pattern previously observed when comparing turbot and model fish genomes and enabled identification of candidate genes related to sexual development and reproduction at LG5, such as DNAJC19 and Sox2. The available number of markers in the turbot map could be easily increased to 592 because of the established correspondence between linkage groups of the two main genetic maps, thus approaching 1 marker/Mb based on the turbot genome size. This map represents a useful tool for evolutionary studies and for supporting ongoing marker-assisted selection programs in this species.
Fil: Hermida, Miguel. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España
Fil: Bouza, Carmen. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España
Fil: Fernández, Carlos. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España
Fil: Sciara, Andres Angel. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Rodríguez Ramilo, Silvia T.. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Mejora Genética Animal; España
Fil: Fernández, Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Mejora Genética Animal; España
Fil: Martinez, Paulino. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departamento de Genética; España
description The turbot (Scophthalmus maximus) is a species of great relevance to European aquaculture and hence might benefit from enhanced genomic resources. The aim of this study was to integrate all previous turbot mapping resources to obtain a dense consensus genetic map. A total of 514 single nucleotide polymorphisms (SNP) and microsatellite markers from reported turbot genetic maps, were used for map construction. Among these markers, 487 (LOD score > 3), including 39% expressed sequence tag (EST)-linked and 61% anonymous, were integrated in 24 linkage groups. The linkage map comprised a total length of 1414 cM (1274.4 cM framework) with 3.3 cM (3.6 cM framework) inter-marker distance, representing an estimated genomic coverage of ~ 90%. This map was used to refine quantitative trait loci (QTL) screening for sex, because of the relevance of this trait for the turbot industry, which confirmed not only the main sex-determining region at linkage group (LG) 5, but also the involvement of additional significant genetic factors at other linkage groups. Comparative mapping supported the macrosyntenic pattern previously observed when comparing turbot and model fish genomes and enabled identification of candidate genes related to sexual development and reproduction at LG5, such as DNAJC19 and Sox2. The available number of markers in the turbot map could be easily increased to 592 because of the established correspondence between linkage groups of the two main genetic maps, thus approaching 1 marker/Mb based on the turbot genome size. This map represents a useful tool for evolutionary studies and for supporting ongoing marker-assisted selection programs in this species.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4858
Hermida, Miguel; Bouza, Carmen; Fernández, Carlos; Sciara, Andres Angel; Rodríguez Ramilo, Silvia T.; et al.; Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map; Elsevier; Aquaculture; 414-415; 11-2013; 19-25
0044-8486
url http://hdl.handle.net/11336/4858
identifier_str_mv Hermida, Miguel; Bouza, Carmen; Fernández, Carlos; Sciara, Andres Angel; Rodríguez Ramilo, Silvia T.; et al.; Compilation of mapping resources in turbot (Scophthalmus maximus): A new integrated consensus genetic map; Elsevier; Aquaculture; 414-415; 11-2013; 19-25
0044-8486
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0044848613003785
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2013.07.040
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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