Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s

Autores
Clop, Eduardo Matias; Chattah, Ana Karina; Perillo, Maria Angelica
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present work was aimed at studying the molecular dynamics at different levels of model membranes having a simulated glycoclix, with focus on the molecular crowding conditions at the lipid–water interfacial region. Thus, binary mixtures of dipalmitoylphosphatidylcholine (dpPC) and a poly(ethylene glycol) (PEGn) derivative of dipalmitoylphosphatidylethanolamine (PE) (where n = 350, 1000, and 5000, respectively, refer to PEG molecular masses) were submitted to 1H spin–lattice relaxation time (T1) and 31P NMR spectra analysis. 1H NMR relaxation times revealed two contributing components in each proton system (PEG, phospholipids, and water), for all the mixtures studied, exhibiting values of T1 with very different orders of magnitude. This allowed identifying confined and bulk water populations as well as PEG moieties becoming more disordered and independent from the phospholipid moiety as n increased. 31P spectra showed a typical broad bilayer signal for n = 350 and 1000, and an isotropic signal characteristic of micelles for n = 5000. Surface pressure (π)–molecular area isotherms and compressional modulus measurements provided further structural information. Moreover, epifluorescence microscopy data from monolayers at π ∼ 30 mN/m, the expected equilibrium π in lipid bilayers, allowed us to postulate that both 1H populations resolved through NMR in phospholipids and lipopolymers corresponded to different phase domains.
Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Chattah, Ana Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Materia
Polymer Grafted Lipids
Pe-Peg
Water Dynamics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32261
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)sClop, Eduardo MatiasChattah, Ana KarinaPerillo, Maria AngelicaPolymer Grafted LipidsPe-PegWater Dynamicshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present work was aimed at studying the molecular dynamics at different levels of model membranes having a simulated glycoclix, with focus on the molecular crowding conditions at the lipid–water interfacial region. Thus, binary mixtures of dipalmitoylphosphatidylcholine (dpPC) and a poly(ethylene glycol) (PEGn) derivative of dipalmitoylphosphatidylethanolamine (PE) (where n = 350, 1000, and 5000, respectively, refer to PEG molecular masses) were submitted to 1H spin–lattice relaxation time (T1) and 31P NMR spectra analysis. 1H NMR relaxation times revealed two contributing components in each proton system (PEG, phospholipids, and water), for all the mixtures studied, exhibiting values of T1 with very different orders of magnitude. This allowed identifying confined and bulk water populations as well as PEG moieties becoming more disordered and independent from the phospholipid moiety as n increased. 31P spectra showed a typical broad bilayer signal for n = 350 and 1000, and an isotropic signal characteristic of micelles for n = 5000. Surface pressure (π)–molecular area isotherms and compressional modulus measurements provided further structural information. Moreover, epifluorescence microscopy data from monolayers at π ∼ 30 mN/m, the expected equilibrium π in lipid bilayers, allowed us to postulate that both 1H populations resolved through NMR in phospholipids and lipopolymers corresponded to different phase domains.Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Chattah, Ana Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaAmerican Chemical Society2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32261Perillo, Maria Angelica; Chattah, Ana Karina; Clop, Eduardo Matias; Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s; American Chemical Society; Journal of Physical Chemistry B; 118; 23; 5-2014; 6150-61581520-6106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/jp410894xinfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/jp410894xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:36Zoai:ri.conicet.gov.ar:11336/32261instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:36.473CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s
title Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s
spellingShingle Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s
Clop, Eduardo Matias
Polymer Grafted Lipids
Pe-Peg
Water Dynamics
title_short Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s
title_full Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s
title_fullStr Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s
title_full_unstemmed Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s
title_sort Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s
dc.creator.none.fl_str_mv Clop, Eduardo Matias
Chattah, Ana Karina
Perillo, Maria Angelica
author Clop, Eduardo Matias
author_facet Clop, Eduardo Matias
Chattah, Ana Karina
Perillo, Maria Angelica
author_role author
author2 Chattah, Ana Karina
Perillo, Maria Angelica
author2_role author
author
dc.subject.none.fl_str_mv Polymer Grafted Lipids
Pe-Peg
Water Dynamics
topic Polymer Grafted Lipids
Pe-Peg
Water Dynamics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present work was aimed at studying the molecular dynamics at different levels of model membranes having a simulated glycoclix, with focus on the molecular crowding conditions at the lipid–water interfacial region. Thus, binary mixtures of dipalmitoylphosphatidylcholine (dpPC) and a poly(ethylene glycol) (PEGn) derivative of dipalmitoylphosphatidylethanolamine (PE) (where n = 350, 1000, and 5000, respectively, refer to PEG molecular masses) were submitted to 1H spin–lattice relaxation time (T1) and 31P NMR spectra analysis. 1H NMR relaxation times revealed two contributing components in each proton system (PEG, phospholipids, and water), for all the mixtures studied, exhibiting values of T1 with very different orders of magnitude. This allowed identifying confined and bulk water populations as well as PEG moieties becoming more disordered and independent from the phospholipid moiety as n increased. 31P spectra showed a typical broad bilayer signal for n = 350 and 1000, and an isotropic signal characteristic of micelles for n = 5000. Surface pressure (π)–molecular area isotherms and compressional modulus measurements provided further structural information. Moreover, epifluorescence microscopy data from monolayers at π ∼ 30 mN/m, the expected equilibrium π in lipid bilayers, allowed us to postulate that both 1H populations resolved through NMR in phospholipids and lipopolymers corresponded to different phase domains.
Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Chattah, Ana Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description The present work was aimed at studying the molecular dynamics at different levels of model membranes having a simulated glycoclix, with focus on the molecular crowding conditions at the lipid–water interfacial region. Thus, binary mixtures of dipalmitoylphosphatidylcholine (dpPC) and a poly(ethylene glycol) (PEGn) derivative of dipalmitoylphosphatidylethanolamine (PE) (where n = 350, 1000, and 5000, respectively, refer to PEG molecular masses) were submitted to 1H spin–lattice relaxation time (T1) and 31P NMR spectra analysis. 1H NMR relaxation times revealed two contributing components in each proton system (PEG, phospholipids, and water), for all the mixtures studied, exhibiting values of T1 with very different orders of magnitude. This allowed identifying confined and bulk water populations as well as PEG moieties becoming more disordered and independent from the phospholipid moiety as n increased. 31P spectra showed a typical broad bilayer signal for n = 350 and 1000, and an isotropic signal characteristic of micelles for n = 5000. Surface pressure (π)–molecular area isotherms and compressional modulus measurements provided further structural information. Moreover, epifluorescence microscopy data from monolayers at π ∼ 30 mN/m, the expected equilibrium π in lipid bilayers, allowed us to postulate that both 1H populations resolved through NMR in phospholipids and lipopolymers corresponded to different phase domains.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32261
Perillo, Maria Angelica; Chattah, Ana Karina; Clop, Eduardo Matias; Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s; American Chemical Society; Journal of Physical Chemistry B; 118; 23; 5-2014; 6150-6158
1520-6106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32261
identifier_str_mv Perillo, Maria Angelica; Chattah, Ana Karina; Clop, Eduardo Matias; Water and Membrane Dynamics in Suspensions of Lipid Vesicles Functionalized with Poly(ethylene glycol)s; American Chemical Society; Journal of Physical Chemistry B; 118; 23; 5-2014; 6150-6158
1520-6106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/jp410894x
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/jp410894x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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