Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface

Autores
Clop, Eduardo Matias; Corvalán, Natalia Andrea; Perillo, Maria Angelica
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The molecular packing-dependent interfacial organization of polyethylene glycol grafted dipalmitoylphosphatidylethanolamine (PE-PEGs) Langmuir films was studied. The PEG chains covered a wide molecular mass range (350, 1000 and 5000 Da).In surface pressure-area (π-A), isotherms PE-PEG1000 and PE-PEG5000 showed transitions (midpoints at πm,t1 ∼ 11 mN/m, "t1"), which appeared as a long non-horizontal line region. Thus, t1 cannot be considered a first-order phase transition but may reflect a transition within the polymer, comprising its desorption from the air-water interface and compaction upon compression. This is supported by the increase in the νs(C-O-C) PM-IRRAS signal intensity and the increasing surface potentials at maximal compression, which reflect thicker polymeric layers. Furthermore, changes in hydrocarbon chain (HC) packing and tilt with respect to the surface led to reorientation in the PO2− group upon compression, indicated by the inversion of the νasym(PO2−) PM-IRRAS signal around t1. The absence of a t1 in PE-PEG350 supports the requisite of a critical polymer chain length for this transition to occur. In-situ epifluorescence microscopy revealed 2D-domain-like structures in PE-PEG1000 and PE-PEG5000 around t1, possibly associated with gelation/dehydration of the polymeric layer and appearing at decreasing π as the polymeric tail became longer.Another transition, t2, appearing in PE-PEG350 and PE-PEG1000 at πm,t2 = 29.4 and 34.8 mN/m, respectively, was associated with HC condensation and was impaired in PE-PEG5000 due to steric hindrance imposed by the large size of its polymer moiety.Two critical lengths of polymer chains were found, one of which allowed the onset of polymeric-tail gelation and the other limited HC compaction.
Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Materia
Polymer Grafted Lipids
Pe-Peg
Mixing Bahavior
Langmuir Films
Pm-Irras
Bam
Efm
Surface Aggregation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42443
Acceder
id CONICETDig_8e180427f544c165428786af5504a5da
oai_identifier_str oai:ri.conicet.gov.ar:11336/42443
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interfaceClop, Eduardo MatiasCorvalán, Natalia AndreaPerillo, Maria AngelicaPolymer Grafted LipidsPe-PegMixing BahaviorLangmuir FilmsPm-IrrasBamEfmSurface Aggregationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The molecular packing-dependent interfacial organization of polyethylene glycol grafted dipalmitoylphosphatidylethanolamine (PE-PEGs) Langmuir films was studied. The PEG chains covered a wide molecular mass range (350, 1000 and 5000 Da).In surface pressure-area (π-A), isotherms PE-PEG1000 and PE-PEG5000 showed transitions (midpoints at πm,t1 ∼ 11 mN/m, "t1"), which appeared as a long non-horizontal line region. Thus, t1 cannot be considered a first-order phase transition but may reflect a transition within the polymer, comprising its desorption from the air-water interface and compaction upon compression. This is supported by the increase in the νs(C-O-C) PM-IRRAS signal intensity and the increasing surface potentials at maximal compression, which reflect thicker polymeric layers. Furthermore, changes in hydrocarbon chain (HC) packing and tilt with respect to the surface led to reorientation in the PO2− group upon compression, indicated by the inversion of the νasym(PO2−) PM-IRRAS signal around t1. The absence of a t1 in PE-PEG350 supports the requisite of a critical polymer chain length for this transition to occur. In-situ epifluorescence microscopy revealed 2D-domain-like structures in PE-PEG1000 and PE-PEG5000 around t1, possibly associated with gelation/dehydration of the polymeric layer and appearing at decreasing π as the polymeric tail became longer.Another transition, t2, appearing in PE-PEG350 and PE-PEG1000 at πm,t2 = 29.4 and 34.8 mN/m, respectively, was associated with HC condensation and was impaired in PE-PEG5000 due to steric hindrance imposed by the large size of its polymer moiety.Two critical lengths of polymer chains were found, one of which allowed the onset of polymeric-tail gelation and the other limited HC compaction.Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; ArgentinaFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaElsevier Science2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42443Clop, Eduardo Matias; Corvalán, Natalia Andrea; Perillo, Maria Angelica; Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 148; 1-12-2016; 640-6490927-7765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776516306774info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2016.09.023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:37:53Zoai:ri.conicet.gov.ar:11336/42443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:37:54.307CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface
title Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface
spellingShingle Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface
Clop, Eduardo Matias
Polymer Grafted Lipids
Pe-Peg
Mixing Bahavior
Langmuir Films
Pm-Irras
Bam
Efm
Surface Aggregation
title_short Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface
title_full Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface
title_fullStr Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface
title_full_unstemmed Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface
title_sort Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface
dc.creator.none.fl_str_mv Clop, Eduardo Matias
Corvalán, Natalia Andrea
Perillo, Maria Angelica
author Clop, Eduardo Matias
author_facet Clop, Eduardo Matias
Corvalán, Natalia Andrea
Perillo, Maria Angelica
author_role author
author2 Corvalán, Natalia Andrea
Perillo, Maria Angelica
author2_role author
author
dc.subject.none.fl_str_mv Polymer Grafted Lipids
Pe-Peg
Mixing Bahavior
Langmuir Films
Pm-Irras
Bam
Efm
Surface Aggregation
topic Polymer Grafted Lipids
Pe-Peg
Mixing Bahavior
Langmuir Films
Pm-Irras
Bam
Efm
Surface Aggregation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The molecular packing-dependent interfacial organization of polyethylene glycol grafted dipalmitoylphosphatidylethanolamine (PE-PEGs) Langmuir films was studied. The PEG chains covered a wide molecular mass range (350, 1000 and 5000 Da).In surface pressure-area (π-A), isotherms PE-PEG1000 and PE-PEG5000 showed transitions (midpoints at πm,t1 ∼ 11 mN/m, "t1"), which appeared as a long non-horizontal line region. Thus, t1 cannot be considered a first-order phase transition but may reflect a transition within the polymer, comprising its desorption from the air-water interface and compaction upon compression. This is supported by the increase in the νs(C-O-C) PM-IRRAS signal intensity and the increasing surface potentials at maximal compression, which reflect thicker polymeric layers. Furthermore, changes in hydrocarbon chain (HC) packing and tilt with respect to the surface led to reorientation in the PO2− group upon compression, indicated by the inversion of the νasym(PO2−) PM-IRRAS signal around t1. The absence of a t1 in PE-PEG350 supports the requisite of a critical polymer chain length for this transition to occur. In-situ epifluorescence microscopy revealed 2D-domain-like structures in PE-PEG1000 and PE-PEG5000 around t1, possibly associated with gelation/dehydration of the polymeric layer and appearing at decreasing π as the polymeric tail became longer.Another transition, t2, appearing in PE-PEG350 and PE-PEG1000 at πm,t2 = 29.4 and 34.8 mN/m, respectively, was associated with HC condensation and was impaired in PE-PEG5000 due to steric hindrance imposed by the large size of its polymer moiety.Two critical lengths of polymer chains were found, one of which allowed the onset of polymeric-tail gelation and the other limited HC compaction.
Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description The molecular packing-dependent interfacial organization of polyethylene glycol grafted dipalmitoylphosphatidylethanolamine (PE-PEGs) Langmuir films was studied. The PEG chains covered a wide molecular mass range (350, 1000 and 5000 Da).In surface pressure-area (π-A), isotherms PE-PEG1000 and PE-PEG5000 showed transitions (midpoints at πm,t1 ∼ 11 mN/m, "t1"), which appeared as a long non-horizontal line region. Thus, t1 cannot be considered a first-order phase transition but may reflect a transition within the polymer, comprising its desorption from the air-water interface and compaction upon compression. This is supported by the increase in the νs(C-O-C) PM-IRRAS signal intensity and the increasing surface potentials at maximal compression, which reflect thicker polymeric layers. Furthermore, changes in hydrocarbon chain (HC) packing and tilt with respect to the surface led to reorientation in the PO2− group upon compression, indicated by the inversion of the νasym(PO2−) PM-IRRAS signal around t1. The absence of a t1 in PE-PEG350 supports the requisite of a critical polymer chain length for this transition to occur. In-situ epifluorescence microscopy revealed 2D-domain-like structures in PE-PEG1000 and PE-PEG5000 around t1, possibly associated with gelation/dehydration of the polymeric layer and appearing at decreasing π as the polymeric tail became longer.Another transition, t2, appearing in PE-PEG350 and PE-PEG1000 at πm,t2 = 29.4 and 34.8 mN/m, respectively, was associated with HC condensation and was impaired in PE-PEG5000 due to steric hindrance imposed by the large size of its polymer moiety.Two critical lengths of polymer chains were found, one of which allowed the onset of polymeric-tail gelation and the other limited HC compaction.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42443
Clop, Eduardo Matias; Corvalán, Natalia Andrea; Perillo, Maria Angelica; Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 148; 1-12-2016; 640-649
0927-7765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42443
identifier_str_mv Clop, Eduardo Matias; Corvalán, Natalia Andrea; Perillo, Maria Angelica; Langmuir films of dipalmitoyl phosphatidylethanolamine grafted poly(ethylene glycol): In-situ evidence of surface aggregation at the air-water interface; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 148; 1-12-2016; 640-649
0927-7765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776516306774
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2016.09.023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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