Neltuma ruscifolia extracts for poultry feed preservation

Autores
Gómez, Analia de Los Angeles; Sgariglia, Melina Araceli; Delgado, Osvaldo Daniel; Soberon, Jose Rodolfo; Bueno, Dante Javier; Sampietro, Diego Alejandro
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neltuma ruscifolia, also known as vinal, is an invasive tree that grows in the semi-arid andsubhumid regions of the South American Chaco. Its abundant leaves are a valuable source ofbioactive constituents, which are currently underutilized. This study examined the ethanolextract (ELE) and its alkaloid fraction (AF) from Neltuma ruscifolia leaves for their phytochemicalprofile and antioxidant activity. Their toxicological and antifungal properties were evaluated,both alone and in combination with potassium sorbate (PS). ELE, ELE+PS, or PS were also addedto a poultry feed to examine their protective impact against oxidative damage and antifungal andantiaflatoxigenic properties against A. flavus. ELE had a total content of phenolic compounds of132 mg of gallic acid equivalent/g, with a significant content of apigenin C-glycosides, mainlyisoschaftoside and schaftoside, and moderate antioxidant activity (IC₅₀ DPPH = 250 mg/L). AF,which was mainly composed of julifloridine, showed negligible antioxidant capacity. ELE and AFsuppressed the growth of Fusarium and Aspergillus strains at concentrations (750–1500 mg/Land 375–750 mg/L, respectively) lower or similar to that of PS (1500 mg/L). They also synergizedthe antifungal activity of PS. Toxicity evaluated by the brine shrimp lethality assay confirmedthat both ELE and its combination with PS are non-toxic (LC₅₀ > 1,000 mg/L). In applicationtrials in poultry feed artificially inoculated with A. flavus, ELE and ELE+PS, at minimuminhibitory concentrations, significantly reduced fungal viability, biomass, and aflatoxincontamination by 93–99%, 60–70%, and 90%, respectively, outperforming PS alone.Additionally, ELE showed a moderate protective effect against feed oxidative damage, lasting forat least one week. These findings suggest that ELE, alone or in combination with PS, is apromising phytogenic additive for poultry feeds, capable of controlling fungal and aflatoxincontamination, as well as oxidative spoilage.
Fil: Gómez, Analia de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Bueno, Dante Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Universidad Autónoma de Entre Ríos; Argentina
Fil: Sampietro, Diego Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
ALKALOIDS
AFLATOXIN
HPLC-DAD
PHENOLIC COMPUND
NELTUMA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272165
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272165
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neltuma ruscifolia extracts for poultry feed preservationGómez, Analia de Los AngelesSgariglia, Melina AraceliDelgado, Osvaldo DanielSoberon, Jose RodolfoBueno, Dante JavierSampietro, Diego AlejandroALKALOIDSAFLATOXINHPLC-DADPHENOLIC COMPUNDNELTUMAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neltuma ruscifolia, also known as vinal, is an invasive tree that grows in the semi-arid andsubhumid regions of the South American Chaco. Its abundant leaves are a valuable source ofbioactive constituents, which are currently underutilized. This study examined the ethanolextract (ELE) and its alkaloid fraction (AF) from Neltuma ruscifolia leaves for their phytochemicalprofile and antioxidant activity. Their toxicological and antifungal properties were evaluated,both alone and in combination with potassium sorbate (PS). ELE, ELE+PS, or PS were also addedto a poultry feed to examine their protective impact against oxidative damage and antifungal andantiaflatoxigenic properties against A. flavus. ELE had a total content of phenolic compounds of132 mg of gallic acid equivalent/g, with a significant content of apigenin C-glycosides, mainlyisoschaftoside and schaftoside, and moderate antioxidant activity (IC₅₀ DPPH = 250 mg/L). AF,which was mainly composed of julifloridine, showed negligible antioxidant capacity. ELE and AFsuppressed the growth of Fusarium and Aspergillus strains at concentrations (750–1500 mg/Land 375–750 mg/L, respectively) lower or similar to that of PS (1500 mg/L). They also synergizedthe antifungal activity of PS. Toxicity evaluated by the brine shrimp lethality assay confirmedthat both ELE and its combination with PS are non-toxic (LC₅₀ > 1,000 mg/L). In applicationtrials in poultry feed artificially inoculated with A. flavus, ELE and ELE+PS, at minimuminhibitory concentrations, significantly reduced fungal viability, biomass, and aflatoxincontamination by 93–99%, 60–70%, and 90%, respectively, outperforming PS alone.Additionally, ELE showed a moderate protective effect against feed oxidative damage, lasting forat least one week. These findings suggest that ELE, alone or in combination with PS, is apromising phytogenic additive for poultry feeds, capable of controlling fungal and aflatoxincontamination, as well as oxidative spoilage.Fil: Gómez, Analia de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Bueno, Dante Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Universidad Autónoma de Entre Ríos; ArgentinaFil: Sampietro, Diego Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaGerman Multidisciplinary Publishing Center2025-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272165Gómez, Analia de Los Angeles; Sgariglia, Melina Araceli; Delgado, Osvaldo Daniel; Soberon, Jose Rodolfo; Bueno, Dante Javier; et al.; Neltuma ruscifolia extracts for poultry feed preservation; German Multidisciplinary Publishing Center; German Journal of Veterinary Research; 5; 3; 8-2025; 18-302703-1322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.gmpc-akademie.de/articles/gjvr/single/282info:eu-repo/semantics/altIdentifier/doi/10.51585/gjvr.2025.3.0145info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:36:28Zoai:ri.conicet.gov.ar:11336/272165instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:36:28.35CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neltuma ruscifolia extracts for poultry feed preservation
title Neltuma ruscifolia extracts for poultry feed preservation
spellingShingle Neltuma ruscifolia extracts for poultry feed preservation
Gómez, Analia de Los Angeles
ALKALOIDS
AFLATOXIN
HPLC-DAD
PHENOLIC COMPUND
NELTUMA
title_short Neltuma ruscifolia extracts for poultry feed preservation
title_full Neltuma ruscifolia extracts for poultry feed preservation
title_fullStr Neltuma ruscifolia extracts for poultry feed preservation
title_full_unstemmed Neltuma ruscifolia extracts for poultry feed preservation
title_sort Neltuma ruscifolia extracts for poultry feed preservation
dc.creator.none.fl_str_mv Gómez, Analia de Los Angeles
Sgariglia, Melina Araceli
Delgado, Osvaldo Daniel
Soberon, Jose Rodolfo
Bueno, Dante Javier
Sampietro, Diego Alejandro
author Gómez, Analia de Los Angeles
author_facet Gómez, Analia de Los Angeles
Sgariglia, Melina Araceli
Delgado, Osvaldo Daniel
Soberon, Jose Rodolfo
Bueno, Dante Javier
Sampietro, Diego Alejandro
author_role author
author2 Sgariglia, Melina Araceli
Delgado, Osvaldo Daniel
Soberon, Jose Rodolfo
Bueno, Dante Javier
Sampietro, Diego Alejandro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALKALOIDS
AFLATOXIN
HPLC-DAD
PHENOLIC COMPUND
NELTUMA
topic ALKALOIDS
AFLATOXIN
HPLC-DAD
PHENOLIC COMPUND
NELTUMA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Neltuma ruscifolia, also known as vinal, is an invasive tree that grows in the semi-arid andsubhumid regions of the South American Chaco. Its abundant leaves are a valuable source ofbioactive constituents, which are currently underutilized. This study examined the ethanolextract (ELE) and its alkaloid fraction (AF) from Neltuma ruscifolia leaves for their phytochemicalprofile and antioxidant activity. Their toxicological and antifungal properties were evaluated,both alone and in combination with potassium sorbate (PS). ELE, ELE+PS, or PS were also addedto a poultry feed to examine their protective impact against oxidative damage and antifungal andantiaflatoxigenic properties against A. flavus. ELE had a total content of phenolic compounds of132 mg of gallic acid equivalent/g, with a significant content of apigenin C-glycosides, mainlyisoschaftoside and schaftoside, and moderate antioxidant activity (IC₅₀ DPPH = 250 mg/L). AF,which was mainly composed of julifloridine, showed negligible antioxidant capacity. ELE and AFsuppressed the growth of Fusarium and Aspergillus strains at concentrations (750–1500 mg/Land 375–750 mg/L, respectively) lower or similar to that of PS (1500 mg/L). They also synergizedthe antifungal activity of PS. Toxicity evaluated by the brine shrimp lethality assay confirmedthat both ELE and its combination with PS are non-toxic (LC₅₀ > 1,000 mg/L). In applicationtrials in poultry feed artificially inoculated with A. flavus, ELE and ELE+PS, at minimuminhibitory concentrations, significantly reduced fungal viability, biomass, and aflatoxincontamination by 93–99%, 60–70%, and 90%, respectively, outperforming PS alone.Additionally, ELE showed a moderate protective effect against feed oxidative damage, lasting forat least one week. These findings suggest that ELE, alone or in combination with PS, is apromising phytogenic additive for poultry feeds, capable of controlling fungal and aflatoxincontamination, as well as oxidative spoilage.
Fil: Gómez, Analia de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Bueno, Dante Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Universidad Autónoma de Entre Ríos; Argentina
Fil: Sampietro, Diego Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Neltuma ruscifolia, also known as vinal, is an invasive tree that grows in the semi-arid andsubhumid regions of the South American Chaco. Its abundant leaves are a valuable source ofbioactive constituents, which are currently underutilized. This study examined the ethanolextract (ELE) and its alkaloid fraction (AF) from Neltuma ruscifolia leaves for their phytochemicalprofile and antioxidant activity. Their toxicological and antifungal properties were evaluated,both alone and in combination with potassium sorbate (PS). ELE, ELE+PS, or PS were also addedto a poultry feed to examine their protective impact against oxidative damage and antifungal andantiaflatoxigenic properties against A. flavus. ELE had a total content of phenolic compounds of132 mg of gallic acid equivalent/g, with a significant content of apigenin C-glycosides, mainlyisoschaftoside and schaftoside, and moderate antioxidant activity (IC₅₀ DPPH = 250 mg/L). AF,which was mainly composed of julifloridine, showed negligible antioxidant capacity. ELE and AFsuppressed the growth of Fusarium and Aspergillus strains at concentrations (750–1500 mg/Land 375–750 mg/L, respectively) lower or similar to that of PS (1500 mg/L). They also synergizedthe antifungal activity of PS. Toxicity evaluated by the brine shrimp lethality assay confirmedthat both ELE and its combination with PS are non-toxic (LC₅₀ > 1,000 mg/L). In applicationtrials in poultry feed artificially inoculated with A. flavus, ELE and ELE+PS, at minimuminhibitory concentrations, significantly reduced fungal viability, biomass, and aflatoxincontamination by 93–99%, 60–70%, and 90%, respectively, outperforming PS alone.Additionally, ELE showed a moderate protective effect against feed oxidative damage, lasting forat least one week. These findings suggest that ELE, alone or in combination with PS, is apromising phytogenic additive for poultry feeds, capable of controlling fungal and aflatoxincontamination, as well as oxidative spoilage.
publishDate 2025
dc.date.none.fl_str_mv 2025-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272165
Gómez, Analia de Los Angeles; Sgariglia, Melina Araceli; Delgado, Osvaldo Daniel; Soberon, Jose Rodolfo; Bueno, Dante Javier; et al.; Neltuma ruscifolia extracts for poultry feed preservation; German Multidisciplinary Publishing Center; German Journal of Veterinary Research; 5; 3; 8-2025; 18-30
2703-1322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272165
identifier_str_mv Gómez, Analia de Los Angeles; Sgariglia, Melina Araceli; Delgado, Osvaldo Daniel; Soberon, Jose Rodolfo; Bueno, Dante Javier; et al.; Neltuma ruscifolia extracts for poultry feed preservation; German Multidisciplinary Publishing Center; German Journal of Veterinary Research; 5; 3; 8-2025; 18-30
2703-1322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.gmpc-akademie.de/articles/gjvr/single/282
info:eu-repo/semantics/altIdentifier/doi/10.51585/gjvr.2025.3.0145
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv German Multidisciplinary Publishing Center
publisher.none.fl_str_mv German Multidisciplinary Publishing Center
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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