Physicochemical study of the formation of complexes between pancreatic proteases and polyanions

Autores
Lombardi, Julia; Picó, Guillermo Alfredo; Boeris, Valeria
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The formation of insoluble complexes between proteins and oppositely charged polyelectrolytes was assessed. Two pancreatic enzymes: trypsin and chymotrypsin, and two anionic synthetic polyelectrolytes: polyacrylate and polyvinylsulfonate, were used for the study at the pH range between 3.00 and 5.00. Two different titration curve shapes, representing two insoluble complexes formation mechanisms, were found. The turbidity of enzyme–polyelectrolyte mixtures is related to the increase either in the size or in the quantity of the insoluble complexes. Ionic strength destabilized insoluble complex formation. Finally, the kinetics of the process of insoluble complex formation at different conditions was studied.
Fil: Lombardi, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Materia
Pe
Polyelectrolytes
Paa
Polyacrylate
Pvs
Polyvinilsulfonate
Tryp
Trypsin
Chtrp
Chymotrypsin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13407
Acceder
id CONICETDig_df8d2e022a0f5f90261aa0f4ff6371b5
oai_identifier_str oai:ri.conicet.gov.ar:11336/13407
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Physicochemical study of the formation of complexes between pancreatic proteases and polyanionsLombardi, JuliaPicó, Guillermo AlfredoBoeris, ValeriaPePolyelectrolytesPaaPolyacrylatePvsPolyvinilsulfonateTrypTrypsinChtrpChymotrypsinhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The formation of insoluble complexes between proteins and oppositely charged polyelectrolytes was assessed. Two pancreatic enzymes: trypsin and chymotrypsin, and two anionic synthetic polyelectrolytes: polyacrylate and polyvinylsulfonate, were used for the study at the pH range between 3.00 and 5.00. Two different titration curve shapes, representing two insoluble complexes formation mechanisms, were found. The turbidity of enzyme–polyelectrolyte mixtures is related to the increase either in the size or in the quantity of the insoluble complexes. Ionic strength destabilized insoluble complex formation. Finally, the kinetics of the process of insoluble complex formation at different conditions was studied.Fil: Lombardi, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaElsevier Science2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13407Lombardi, Julia; Picó, Guillermo Alfredo; Boeris, Valeria; Physicochemical study of the formation of complexes between pancreatic proteases and polyanions; Elsevier Science; International Journal Of Biological Macromolecules; 79; 8-2015; 160-1660141-8130enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2015.04.034info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S014181301500272Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:29:33Zoai:ri.conicet.gov.ar:11336/13407instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:29:33.404CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
title Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
spellingShingle Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
Lombardi, Julia
Pe
Polyelectrolytes
Paa
Polyacrylate
Pvs
Polyvinilsulfonate
Tryp
Trypsin
Chtrp
Chymotrypsin
title_short Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
title_full Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
title_fullStr Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
title_full_unstemmed Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
title_sort Physicochemical study of the formation of complexes between pancreatic proteases and polyanions
dc.creator.none.fl_str_mv Lombardi, Julia
Picó, Guillermo Alfredo
Boeris, Valeria
author Lombardi, Julia
author_facet Lombardi, Julia
Picó, Guillermo Alfredo
Boeris, Valeria
author_role author
author2 Picó, Guillermo Alfredo
Boeris, Valeria
author2_role author
author
dc.subject.none.fl_str_mv Pe
Polyelectrolytes
Paa
Polyacrylate
Pvs
Polyvinilsulfonate
Tryp
Trypsin
Chtrp
Chymotrypsin
topic Pe
Polyelectrolytes
Paa
Polyacrylate
Pvs
Polyvinilsulfonate
Tryp
Trypsin
Chtrp
Chymotrypsin
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The formation of insoluble complexes between proteins and oppositely charged polyelectrolytes was assessed. Two pancreatic enzymes: trypsin and chymotrypsin, and two anionic synthetic polyelectrolytes: polyacrylate and polyvinylsulfonate, were used for the study at the pH range between 3.00 and 5.00. Two different titration curve shapes, representing two insoluble complexes formation mechanisms, were found. The turbidity of enzyme–polyelectrolyte mixtures is related to the increase either in the size or in the quantity of the insoluble complexes. Ionic strength destabilized insoluble complex formation. Finally, the kinetics of the process of insoluble complex formation at different conditions was studied.
Fil: Lombardi, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
description The formation of insoluble complexes between proteins and oppositely charged polyelectrolytes was assessed. Two pancreatic enzymes: trypsin and chymotrypsin, and two anionic synthetic polyelectrolytes: polyacrylate and polyvinylsulfonate, were used for the study at the pH range between 3.00 and 5.00. Two different titration curve shapes, representing two insoluble complexes formation mechanisms, were found. The turbidity of enzyme–polyelectrolyte mixtures is related to the increase either in the size or in the quantity of the insoluble complexes. Ionic strength destabilized insoluble complex formation. Finally, the kinetics of the process of insoluble complex formation at different conditions was studied.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13407
Lombardi, Julia; Picó, Guillermo Alfredo; Boeris, Valeria; Physicochemical study of the formation of complexes between pancreatic proteases and polyanions; Elsevier Science; International Journal Of Biological Macromolecules; 79; 8-2015; 160-166
0141-8130
url http://hdl.handle.net/11336/13407
identifier_str_mv Lombardi, Julia; Picó, Guillermo Alfredo; Boeris, Valeria; Physicochemical study of the formation of complexes between pancreatic proteases and polyanions; Elsevier Science; International Journal Of Biological Macromolecules; 79; 8-2015; 160-166
0141-8130
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2015.04.034
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S014181301500272X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781882002309120
score 12.982451

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