Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate

Autores
Lombardi, Julia; Woitovich Valetti, Nadia; Picó, Guillermo Alfredo; Boeris, Valeria
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Serine proteases have wide application in leather, food, meat and soap powder industries, among others. There are a lot of methodologies to obtain them in large quantities but most of them are expensive or contaminating. We then propose an economical and environmentally friendly method in which proteases are separated from a crude fresh bovine pancreas homogenate using precipitation with polyacrylate, a commercially available negatively charged and weak polyelectrolyte. The zymogens of the serine proteases were activated prior to precipitation by the addition of trypsin. The proteases were precipitated by adding polyacrylate at pH 4.50 to the pancreas homogenate. Under these conditions, serine proteases are positively charged and form non-soluble complexes with the negatively charged polyacrylate. The purity of proteases was increased 5-fold with a recovery of 33% under the best conditions tested. The volume of the final product was decreased to 5% of the feedstock, in order to concentrate the sample up to 20 times. The proposed method removed up to 96% of the contaminant proteins.
Fil: Lombardi, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Woitovich Valetti, Nadia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Materia
Bovine Pancreas Proteases
Serine Proteases
Trypsin
Chymotrypsin
Polyelectrolyte
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7471
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7471
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylateLombardi, JuliaWoitovich Valetti, NadiaPicó, Guillermo AlfredoBoeris, ValeriaBovine Pancreas ProteasesSerine ProteasesTrypsinChymotrypsinPolyelectrolytehttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Serine proteases have wide application in leather, food, meat and soap powder industries, among others. There are a lot of methodologies to obtain them in large quantities but most of them are expensive or contaminating. We then propose an economical and environmentally friendly method in which proteases are separated from a crude fresh bovine pancreas homogenate using precipitation with polyacrylate, a commercially available negatively charged and weak polyelectrolyte. The zymogens of the serine proteases were activated prior to precipitation by the addition of trypsin. The proteases were precipitated by adding polyacrylate at pH 4.50 to the pancreas homogenate. Under these conditions, serine proteases are positively charged and form non-soluble complexes with the negatively charged polyacrylate. The purity of proteases was increased 5-fold with a recovery of 33% under the best conditions tested. The volume of the final product was decreased to 5% of the feedstock, in order to concentrate the sample up to 20 times. The proposed method removed up to 96% of the contaminant proteins.Fil: Lombardi, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Woitovich Valetti, Nadia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaElsevier2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7471Lombardi, Julia; Woitovich Valetti, Nadia; Picó, Guillermo Alfredo; Boeris, Valeria; Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate; Elsevier; Separation And Purification Technology; 116; 9-2013; 170-1741383-5866enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383586613003407info:eu-repo/semantics/altIdentifier/doi/10.1016/j.seppur.2013.05.047info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:01:06Zoai:ri.conicet.gov.ar:11336/7471instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:01:06.301CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate
title Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate
spellingShingle Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate
Lombardi, Julia
Bovine Pancreas Proteases
Serine Proteases
Trypsin
Chymotrypsin
Polyelectrolyte
title_short Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate
title_full Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate
title_fullStr Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate
title_full_unstemmed Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate
title_sort Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate
dc.creator.none.fl_str_mv Lombardi, Julia
Woitovich Valetti, Nadia
Picó, Guillermo Alfredo
Boeris, Valeria
author Lombardi, Julia
author_facet Lombardi, Julia
Woitovich Valetti, Nadia
Picó, Guillermo Alfredo
Boeris, Valeria
author_role author
author2 Woitovich Valetti, Nadia
Picó, Guillermo Alfredo
Boeris, Valeria
author2_role author
author
author
dc.subject.none.fl_str_mv Bovine Pancreas Proteases
Serine Proteases
Trypsin
Chymotrypsin
Polyelectrolyte
topic Bovine Pancreas Proteases
Serine Proteases
Trypsin
Chymotrypsin
Polyelectrolyte
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Serine proteases have wide application in leather, food, meat and soap powder industries, among others. There are a lot of methodologies to obtain them in large quantities but most of them are expensive or contaminating. We then propose an economical and environmentally friendly method in which proteases are separated from a crude fresh bovine pancreas homogenate using precipitation with polyacrylate, a commercially available negatively charged and weak polyelectrolyte. The zymogens of the serine proteases were activated prior to precipitation by the addition of trypsin. The proteases were precipitated by adding polyacrylate at pH 4.50 to the pancreas homogenate. Under these conditions, serine proteases are positively charged and form non-soluble complexes with the negatively charged polyacrylate. The purity of proteases was increased 5-fold with a recovery of 33% under the best conditions tested. The volume of the final product was decreased to 5% of the feedstock, in order to concentrate the sample up to 20 times. The proposed method removed up to 96% of the contaminant proteins.
Fil: Lombardi, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Woitovich Valetti, Nadia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
description Serine proteases have wide application in leather, food, meat and soap powder industries, among others. There are a lot of methodologies to obtain them in large quantities but most of them are expensive or contaminating. We then propose an economical and environmentally friendly method in which proteases are separated from a crude fresh bovine pancreas homogenate using precipitation with polyacrylate, a commercially available negatively charged and weak polyelectrolyte. The zymogens of the serine proteases were activated prior to precipitation by the addition of trypsin. The proteases were precipitated by adding polyacrylate at pH 4.50 to the pancreas homogenate. Under these conditions, serine proteases are positively charged and form non-soluble complexes with the negatively charged polyacrylate. The purity of proteases was increased 5-fold with a recovery of 33% under the best conditions tested. The volume of the final product was decreased to 5% of the feedstock, in order to concentrate the sample up to 20 times. The proposed method removed up to 96% of the contaminant proteins.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7471
Lombardi, Julia; Woitovich Valetti, Nadia; Picó, Guillermo Alfredo; Boeris, Valeria; Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate; Elsevier; Separation And Purification Technology; 116; 9-2013; 170-174
1383-5866
url http://hdl.handle.net/11336/7471
identifier_str_mv Lombardi, Julia; Woitovich Valetti, Nadia; Picó, Guillermo Alfredo; Boeris, Valeria; Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate; Elsevier; Separation And Purification Technology; 116; 9-2013; 170-174
1383-5866
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383586613003407
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.seppur.2013.05.047
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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