Role of allopregnanolone on cerebellar granule cells neurogenesis

Autores
Keller, Elizabeth Ana; Zamparini, A.; Borodinsky, L.N.; Gravielle, Maria Clara; Fiszman, Monica Lidia
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of allopregnanolone on immature cerebellar granule cells (CGC) proliferation was studied. Allopregnanolone (0.1–1 μM) increased [3H]thymidine incorporation and cell number determined by neuronal counting and by an MTT colorimetric assay. The effect of the neurosteroid was completely prevented by preincubation with 10 mM MgCl2, 10 μM nifedipine, 10 μM picrotoxin or by 50 μM bicuculine. We conclude that ALLO affects cerebellar neurogenesis by increasing calcium influx through voltage-gated calcium channels and GABAA receptors activation.
Fil: Keller, Elizabeth. ININFA; Argentina
Fil: Zamparini, Andrea. ININFA; Argentina
Fil: Borodinski, Laura. FACULTAD; Argentina
Fil: Gravielle, Maria Clara. ININFA; Argentina
Fil: Fiszman, Monica Lidia. ININFA; Argentina
Materia
ALLOPREGNANOLONE
CALCIUM
CEREBELLUM
GABA A RECEPTOR
NEUROGENESIS
PROLIFERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94394
Acceder
id CONICETDig_9351639b0b95bebbe9bb5af4c62afb52
oai_identifier_str oai:ri.conicet.gov.ar:11336/94394
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of allopregnanolone on cerebellar granule cells neurogenesisKeller, Elizabeth AnaZamparini, A.Borodinsky, L.N.Gravielle, Maria ClaraFiszman, Monica LidiaALLOPREGNANOLONECALCIUMCEREBELLUMGABA A RECEPTORNEUROGENESISPROLIFERATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The role of allopregnanolone on immature cerebellar granule cells (CGC) proliferation was studied. Allopregnanolone (0.1–1 μM) increased [3H]thymidine incorporation and cell number determined by neuronal counting and by an MTT colorimetric assay. The effect of the neurosteroid was completely prevented by preincubation with 10 mM MgCl2, 10 μM nifedipine, 10 μM picrotoxin or by 50 μM bicuculine. We conclude that ALLO affects cerebellar neurogenesis by increasing calcium influx through voltage-gated calcium channels and GABAA receptors activation.Fil: Keller, Elizabeth. ININFA; ArgentinaFil: Zamparini, Andrea. ININFA; ArgentinaFil: Borodinski, Laura. FACULTAD; ArgentinaFil: Gravielle, Maria Clara. ININFA; ArgentinaFil: Fiszman, Monica Lidia. ININFA; ArgentinaElsevier2004-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94394Keller, Elizabeth Ana; Zamparini, A.; Borodinsky, L.N.; Gravielle, Maria Clara; Fiszman, Monica Lidia; Role of allopregnanolone on cerebellar granule cells neurogenesis; Elsevier; Developmental Brain Research; 153; 1; 10-2004; 13-170165-3806CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165380604002329info:eu-repo/semantics/altIdentifier/doi/10.1016/j.devbrainres.2004.07.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:17Zoai:ri.conicet.gov.ar:11336/94394instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:18.2CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of allopregnanolone on cerebellar granule cells neurogenesis
title Role of allopregnanolone on cerebellar granule cells neurogenesis
spellingShingle Role of allopregnanolone on cerebellar granule cells neurogenesis
Keller, Elizabeth Ana
ALLOPREGNANOLONE
CALCIUM
CEREBELLUM
GABA A RECEPTOR
NEUROGENESIS
PROLIFERATION
title_short Role of allopregnanolone on cerebellar granule cells neurogenesis
title_full Role of allopregnanolone on cerebellar granule cells neurogenesis
title_fullStr Role of allopregnanolone on cerebellar granule cells neurogenesis
title_full_unstemmed Role of allopregnanolone on cerebellar granule cells neurogenesis
title_sort Role of allopregnanolone on cerebellar granule cells neurogenesis
dc.creator.none.fl_str_mv Keller, Elizabeth Ana
Zamparini, A.
Borodinsky, L.N.
Gravielle, Maria Clara
Fiszman, Monica Lidia
author Keller, Elizabeth Ana
author_facet Keller, Elizabeth Ana
Zamparini, A.
Borodinsky, L.N.
Gravielle, Maria Clara
Fiszman, Monica Lidia
author_role author
author2 Zamparini, A.
Borodinsky, L.N.
Gravielle, Maria Clara
Fiszman, Monica Lidia
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALLOPREGNANOLONE
CALCIUM
CEREBELLUM
GABA A RECEPTOR
NEUROGENESIS
PROLIFERATION
topic ALLOPREGNANOLONE
CALCIUM
CEREBELLUM
GABA A RECEPTOR
NEUROGENESIS
PROLIFERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The role of allopregnanolone on immature cerebellar granule cells (CGC) proliferation was studied. Allopregnanolone (0.1–1 μM) increased [3H]thymidine incorporation and cell number determined by neuronal counting and by an MTT colorimetric assay. The effect of the neurosteroid was completely prevented by preincubation with 10 mM MgCl2, 10 μM nifedipine, 10 μM picrotoxin or by 50 μM bicuculine. We conclude that ALLO affects cerebellar neurogenesis by increasing calcium influx through voltage-gated calcium channels and GABAA receptors activation.
Fil: Keller, Elizabeth. ININFA; Argentina
Fil: Zamparini, Andrea. ININFA; Argentina
Fil: Borodinski, Laura. FACULTAD; Argentina
Fil: Gravielle, Maria Clara. ININFA; Argentina
Fil: Fiszman, Monica Lidia. ININFA; Argentina
description The role of allopregnanolone on immature cerebellar granule cells (CGC) proliferation was studied. Allopregnanolone (0.1–1 μM) increased [3H]thymidine incorporation and cell number determined by neuronal counting and by an MTT colorimetric assay. The effect of the neurosteroid was completely prevented by preincubation with 10 mM MgCl2, 10 μM nifedipine, 10 μM picrotoxin or by 50 μM bicuculine. We conclude that ALLO affects cerebellar neurogenesis by increasing calcium influx through voltage-gated calcium channels and GABAA receptors activation.
publishDate 2004
dc.date.none.fl_str_mv 2004-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94394
Keller, Elizabeth Ana; Zamparini, A.; Borodinsky, L.N.; Gravielle, Maria Clara; Fiszman, Monica Lidia; Role of allopregnanolone on cerebellar granule cells neurogenesis; Elsevier; Developmental Brain Research; 153; 1; 10-2004; 13-17
0165-3806
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94394
identifier_str_mv Keller, Elizabeth Ana; Zamparini, A.; Borodinsky, L.N.; Gravielle, Maria Clara; Fiszman, Monica Lidia; Role of allopregnanolone on cerebellar granule cells neurogenesis; Elsevier; Developmental Brain Research; 153; 1; 10-2004; 13-17
0165-3806
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165380604002329
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.devbrainres.2004.07.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268966029885440
score 13.13397

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